Ventromedial hypothalamic lesions induce the proliferation of gastrointestinal mucosal cells in the rat.

Abstract:

:We reported recently that ventromedial hypothalamic (VMH) lesions increased the synthesis of DNA in the gastrointestinal tract of rats by the firing of vagus nerve activity, mainly via cholinergic receptor mechanisms. In the present study, we examined whether the mitotic response is due to proliferation of a cell population--mucosal, submucosal, or muscular layer. A monoclonal antibody to proliferating cell nuclear antigen (PCNA) has previously been shown to be capable of identifying proliferating cells. Samples of formalin-fixed gastrointestinal epithelium, taken before and after VMH lesioning, were immunostained with the anti-PCNA monoclonal antibody, and the labeling index (LI) was determined. To discriminate the effect of hyperphagia in VMH lesioned rats, we utilized the method of pair-feeding. Cell proliferation was examined by the PCNA-labeling technique 0, 1, 3, and 7 days after VMH lesioning. The increase in proliferation was confined to cells in the mucosa and did not involve the muscularis and serosa. Studies in control animals showed that the LI was higher in the small intestine than in other gut segments, and higher in the large intestine than in the stomach. The mean PCNA-LI began to increase at 1 day and continued to increase for 3 days, then decreased 7 days following the lesioning. Results indicate that the gastrointestinal mucosa is in a state of hyperproliferation after VMH lesioning.

journal_name

Life Sci

journal_title

Life sciences

authors

Kiba T,Tanaka K,Hoshino M,Numata K,Okano K,Inoue S

doi

10.1016/0024-3205(95)02014-a

subject

Has Abstract

pub_date

1995-01-01 00:00:00

pages

827-32

issue

9

eissn

0024-3205

issn

1879-0631

pii

002432059502014A

journal_volume

57

pub_type

杂志文章
  • Alcohol-induced oxidative stress.

    abstract::Alcohol-induced oxidative stress is linked to the metabolism of ethanol involving both microsomal and mitochondrial systems. Ethanol metabolism is directly involved in the production of reactive oxygen species (ROS) and reactive nitrogen species (RNS). These form an environment favourable to oxidative stress. Ethanol ...

    journal_title:Life sciences

    pub_type: 杂志文章,评审

    doi:10.1016/j.lfs.2007.05.005

    authors: Das SK,Vasudevan DM

    更新日期:2007-06-27 00:00:00

  • MiR-27b suppresses epithelial-mesenchymal transition and chemoresistance in lung cancer by targeting Snail1.

    abstract:HEADING AIMS:MicroRNA-27b (miR-27b) has been shown to play a role in the progression of many different forms of cancer, but its specific relevance in the context of non-small cell lung cancer (NSCLC) remains uncertain. As such, this study sought to explore the role of miR-27b in NSCLC and the mechanisms whereby it func...

    journal_title:Life sciences

    pub_type: 杂志文章

    doi:10.1016/j.lfs.2019.117238

    authors: Zhang J,Hua X,Qi N,Han G,Yu J,Yu Y,Wei X,Li H,Chen X,Leng C,Liu Q,Lu Y,Li Y

    更新日期:2020-08-01 00:00:00

  • Brain serotonin and blood pressure regulation: studies using in vivo electrochemistry and direct tissue assay.

    abstract::Hypotensive responses to tryptophan and 5-hydroxytryptophan infusions were studied in normotensive male Sprague-Dawley rats. Results showed that 5-hydroxytryptophan but not tryptophan lowered pressure in a dose dependent way in direct relation to the production of brain serotonin and 5-HIAA. Intrinsic release of serot...

    journal_title:Life sciences

    pub_type: 杂志文章

    doi:10.1016/0024-3205(85)90220-6

    authors: Freed CR,Echizen H,Bhaskaran D

    更新日期:1985-11-11 00:00:00

  • Presence and some characterization of GDP dissociation inhibitors for a low Mr GTP-binding protein, ram p25, in rat spleen cytosol.

    abstract::Two proteinous factors, designated here as ram p25 GDP dissociation inhibitor (I) and (II) (ram-GDI (I) and (II)), were detected in the cytosolic fraction of rat spleen, which inhibited the initial dissociation of GDP from ram p25 produced by E. coli by causing characteristic lag. They had very weak effects on the rat...

    journal_title:Life sciences

    pub_type: 杂志文章

    doi:10.1016/0024-3205(92)90455-x

    authors: Nagata K,Suzuki T,Okano Y,Nozawa Y

    更新日期:1992-01-01 00:00:00

  • Age-related reduction of extrastriatal dopamine D2 receptor measured by PET.

    abstract::Although the aging effect of dopamine D2 receptor in the striatum is well-documented, the effect of age on the extrastriatal dopamine D2 receptor has not been fully examined. Since the density of extrastriatal dopamine D2 receptor is very low, suitable ligands are limited. In this study, we used [11C]FLB 457 to quanti...

    journal_title:Life sciences

    pub_type: 杂志文章

    doi:10.1016/s0024-3205(01)01205-x

    authors: Inoue M,Suhara T,Sudo Y,Okubo Y,Yasuno F,Kishimoto T,Yoshikawa K,Tanada S

    更新日期:2001-07-20 00:00:00

  • Additive effects of combined valsartan and spironolactone on cardiac aldosterone escape in spontaneously hypertensive rats.

    abstract::The additive effects of combined valsartan and spironolactone on plasma and cardiac aldosterone escape were evaluated in spontaneously hypertensive rats (SHRs). Twenty-four SHRs were treated with valsartan (30 mg/kg body weight per day), spironolactone (20 mg/kg body weight per day) and a combination of both for 4 mon...

    journal_title:Life sciences

    pub_type: 杂志文章

    doi:10.1016/j.lfs.2004.04.012

    authors: Liang YH,Wang JM,Zhou Y,Jiang XJ,Jiang H,Huang CX

    更新日期:2004-08-27 00:00:00

  • A lysine-free mutant of epidermal growth factor as targeting moiety of a targeted toxin.

    abstract:AIMS:Elevated levels of epidermal growth factor (EGF) receptor are observed on several human tumors, e.g. cervical carcinoma and mamma carcinomas. The natural ligand EGF is an alternative to established antibodies and tyrosine kinase inhibitors for targeting EGF receptor-overexpressing tumor cells for therapy. Conjugat...

    journal_title:Life sciences

    pub_type: 杂志文章

    doi:10.1016/j.lfs.2010.11.012

    authors: Bachran C,Schneider S,Riese SB,Bachran D,Urban R,Schellmann N,Zahn C,Sutherland M,Fuchs H

    更新日期:2011-01-31 00:00:00

  • Ventricular hypertrophy and arterial hemodynamics following deprivation of nitric oxide in rats.

    abstract::In the present study, we elucidated the possible role of hemodynamic parameters and chemical factors in the development of ventricular hypertrophy (VH) following chronic nitric oxide (NO) deprivation with Nomega-nitro-L-arginine methyl ester (L-NAME). Impedance spectral analysis was used to obtain the arterial hemodyn...

    journal_title:Life sciences

    pub_type: 杂志文章

    doi:10.1016/j.lfs.2005.04.061

    authors: Hu CT,Chang HR,Hsu YH,Liu CJ,Chen HI

    更新日期:2005-11-26 00:00:00

  • Action of pro-opiomelanocortin products on the rat vas deferens.

    abstract::Behavioral study of pro-opiomelanocortin products indicates that beta-endorphin and corticotrophin-like peptides have antagonistic effects. However, these peptides have similar actions on the rat vas deferens. beta-endorphin, alpha-MSH and ACTH each inhibit electrically evoked contraction of the duct, but the corticot...

    journal_title:Life sciences

    pub_type: 杂志文章

    doi:10.1016/0024-3205(86)90162-1

    authors: Smock T

    更新日期:1986-11-03 00:00:00

  • Pathways and roadblocks in muscarinic receptor-mediated growth regulation.

    abstract::In some cell systems muscarinic receptor stimulation can induce proliferation or transformation. This phenomenon is subtype-specific (only m1 and m3 receptors are effective) and cell type dependent. In 1321N1 astrocytoma cells activation of m3 receptors stimulates phospholipase C, but does not induce DNA synthesis. In...

    journal_title:Life sciences

    pub_type: 杂志文章,评审

    doi:

    authors: Brown JH,Sah V,Moskowitz S,Ramirez T,Collins L,Post G,Goldstein D

    更新日期:1997-01-01 00:00:00

  • Chronic intermittent hypobaric hypoxia protects vascular endothelium by ameliorating autophagy in metabolic syndrome rats.

    abstract:AIMS:The study aimed to investigate the protective effect of chronic intermittent hypobaric hypoxia (CIHH) on endothelium function and relaxation of mesenteric artery in metabolism syndrome (MS) rats. MAIN METHODS:Male adult Sprague-Dawley rats were randomly divided into control (CON), CIHH (treated with 28-days hypob...

    journal_title:Life sciences

    pub_type: 杂志文章

    doi:10.1016/j.lfs.2018.05.008

    authors: Cui F,Guan Y,Guo J,Tian YM,Hu HF,Zhang XJ,Zhang Y

    更新日期:2018-07-15 00:00:00

  • Metformin reduces the Walker-256 tumor development in obese-MSG rats via AMPK and FOXO3a.

    abstract:AIMS:Studies have associated obesity with a wide variety of cancers. Metformin, an anti-diabetic drug, has recently received attention as a potentially useful therapeutic agent for treating cancer. Therefore, the objective of this study was to analyze the mechanisms involved in the increase in tumor development and the...

    journal_title:Life sciences

    pub_type: 杂志文章

    doi:10.1016/j.lfs.2014.11.028

    authors: de Queiroz EA,Akamine EH,de Carvalho MH,Sampaio SC,Fortes ZB

    更新日期:2015-01-15 00:00:00

  • Calcitonin inhibits accumulation of cyclic AMP in stimulated peritoneal macrophages from normal rats but not from osteopetrotic (incisors-absent) littermates.

    abstract::Macrophages and osteoclasts derive from related cell lines. In osteopetrotic mutants the function of osteoclasts is greatly reduced compared to that in normal animals or children and macrophage function is variably affected depending upon the mutation. To further explore macrophage function in osteopetrosis we examine...

    journal_title:Life sciences

    pub_type: 杂志文章

    doi:10.1016/0024-3205(89)90575-4

    authors: Popoff SN,Coderre JA,Marks SC Jr,Stock JL

    更新日期:1989-01-01 00:00:00

  • Effect of cocaine and murine AIDS on lamina propria T and B cells in normal mice.

    abstract::We developed an experimental model to study the effect of daily cocaine administration on the mucosal immune system during murine acquired immune deficiency syndrome (MAIDS). Mice were infected with LP-BM5 murine leukemia virus, a retrovirus which causes immunosuppression with development of functional murine AIDS. Mi...

    journal_title:Life sciences

    pub_type: 杂志文章

    doi:10.1016/0024-3205(94)00874-4

    authors: Lopez MC,Watson RR

    更新日期:1994-01-01 00:00:00

  • Apparent diffusion coefficient in the aging mouse brain: a magnetic resonance imaging study.

    abstract::Novel magnetic resonance imaging sequences have and still continue to play an increasing role in neuroimaging and neuroscience. Among these techniques, diffusion-weighted imaging (DWI) has revolutionized the diagnosis and management of diseases such as stroke, neoplastic disease and inflammation. However, the effects ...

    journal_title:Life sciences

    pub_type: 杂志文章

    doi:10.1016/j.lfs.2005.06.032

    authors: Rau PR,Sellner J,Heiland S,Plaschke K,Schellinger PD,Meyding-Lamadé UK,Lamadé WR

    更新日期:2006-02-09 00:00:00

  • In vitro pharmacological profile of peptide III-BTD: a novel ligand for nociceptin/orphanin FQ and opioid receptors.

    abstract::Peptide III-BTD has been recently identified as a non-selective nociceptin/orphanin FQ receptor ligand by screening of a synthetic peptide combinatorial library. In the present study we evaluated the pharmacological profile of peptide III-BTD in isolated tissues (mouse and rat vas deferens, guinea pig ileum) sensitive...

    journal_title:Life sciences

    pub_type: 杂志文章

    doi:10.1016/s0024-3205(00)00931-0

    authors: Bigoni R,Rizzi A,Rizzi D,Becker JA,Kieffer BL,Simonin F,Regoli D,Calo G

    更新日期:2000-12-01 00:00:00

  • Two pools of beta-endorphin-like immunoreactivity in blood: plasma and erythrocytes.

    abstract::While attempting to delineate the reason for the reported extreme variability of beta-endorphin-like immunoreactivity (beta-ir) in human plasma (eg., nondetectable to 1 ng/ml) by standard radioimmunoassay, we noted that a substantial portion of circulating beta-ir was associated with erythrocytes. That erythrocyte ass...

    journal_title:Life sciences

    pub_type: 杂志文章

    doi:10.1016/0024-3205(84)90677-5

    authors: Fisher A,Comly M,Do R,Tamarkin L,Ghazanfari AF,Mukherjee AB

    更新日期:1984-05-07 00:00:00

  • Effects of the cannabinoid receptor agonist CP 55,940 and the cannabinoid receptor antagonist SR 141716 on intracranial self-stimulation in Lewis rats.

    abstract::Lewis rats were trained to self-stimulate the medial forebrain bundle (MFB) using a rate-frequency paradigm. They were then tested for the effects of the cannabinoid receptor agonist CP 55,940, the selective cannabinoid receptor antagonist SR 141716 and the dopamine D1 receptor antagonist SCH 23390. CP 55,940 (0, 10, ...

    journal_title:Life sciences

    pub_type: 杂志文章

    doi:10.1016/s0024-3205(01)01366-2

    authors: Arnold JC,Hunt GE,McGregor IS

    更新日期:2001-11-21 00:00:00

  • Prostaglandin involvement in hyperthermia induced by sleep deprivation: a pharmacological and autoradiographic study.

    abstract:AIMS:Hyperthermia is a characteristic functional effect of sleep deprivation (SD). We hypothesize here that prostaglandin E2 (PGE2) could be involved in hyperthermia induced by sleep deprivation. MAIN METHODS:To address this issue we examined the effects of a selective cyclo-oxygenase-2 inhibitor (COX-2) agent on hype...

    journal_title:Life sciences

    pub_type: 杂志文章

    doi:10.1016/j.lfs.2008.12.009

    authors: Palma BD,Nobrega JN,Gomes VL,Esumi LA,Seabra ML,Tufik S,Hipolide DC

    更新日期:2009-02-27 00:00:00

  • Ubiquitination in rheumatoid arthritis.

    abstract::Rheumatoid arthritis is a chronic, inflammatory joint disease leading to inflammation of synovial membrane that lines the joints. This inflammation further progresses and results in destruction of joints and surrounding cartilages. The underlying factors can be oxidative stress, pro-inflammatory mediators, imbalance a...

    journal_title:Life sciences

    pub_type: 杂志文章,评审

    doi:10.1016/j.lfs.2020.118459

    authors: Behl T,Chadha S,Sachdeva M,Kumar A,Hafeez A,Mehta V,Bungau S

    更新日期:2020-11-15 00:00:00

  • Subcellular distribution of choline acetyltransferase by immunogold electron microscopy in non-neuronal cells: placenta, airways and murine embryonic stem cells.

    abstract:AIMS:Acetylcholine is synthesized in more or less all mammalian cells. However, little is known about the subcellular location of acetylcholine synthesis. Therefore, in the present experiments the subcellular location of the synthesizing enzyme choline acetyltransferase (ChAT) was investigated by anti-ChAT immunogold e...

    journal_title:Life sciences

    pub_type: 杂志文章

    doi:10.1016/j.lfs.2012.05.012

    authors: Wessler I,Michel-Schmidt R,Brochhausen C,Kirkpatrick CJ

    更新日期:2012-11-27 00:00:00

  • In vivo therapy of myocardial infarction with mesenchymal stem cells modified with prostaglandin I synthase gene improves cardiac performance in mice.

    abstract:AIM:Intra-myocardial injection of adult bone marrow-derived stem cells (MSC) has recently been proposed as a therapy to repair damaged cardiomyocytes after acute myocardial infarction (AMI). PGI(2) has vasodilatation effects; however, the effects of combining both MSC and PGI(2) therapy on AMI have never been evaluated...

    journal_title:Life sciences

    pub_type: 杂志文章

    doi:10.1016/j.lfs.2010.12.020

    authors: Lian WS,Cheng WT,Cheng CC,Hsiao FS,Chen JJ,Cheng CF,Wu SC

    更新日期:2011-02-28 00:00:00

  • Study on the metabolism of lyso-platelet activating factor (lyso-PAF) in human paranasal sinus mucosa. The cultured ciliated epithelium can convert lyso-PAF to PAF.

    abstract::The conversion of lyso-platelet activating factor (lyso-PAF) to PAF in cultured paranasal sinus mucosa obtained from normal human subjects was studied. The PAF concentration in the medium was determined after addition of lyso-PAF. PAF became detectable at 10 minutes after the addition of 10(-8)M lyso-PAF, and reached ...

    journal_title:Life sciences

    pub_type: 杂志文章

    doi:10.1016/0024-3205(93)90483-j

    authors: Inoue H,Ganbo T,Hisamatsu K,Ishida H

    更新日期:1993-01-01 00:00:00

  • Protein kinases C isozymes are differentially expressed in human breast carcinomas.

    abstract:AIMS:The protein kinase C (PKC) family of enzymes has been implicated in cellular proliferation, differentiation, and apoptosis. However, the distribution of specific PKC isoforms with varying functions in normal and malignant human tissues remains to be determined. The objective of this study was to investigate the ex...

    journal_title:Life sciences

    pub_type: 杂志文章

    doi:10.1016/j.lfs.2009.03.007

    authors: Ali S,Al-Sukhun S,El-Rayes BF,Sarkar FH,Heilbrun LK,Philip PA

    更新日期:2009-05-22 00:00:00

  • A comparison of the central and peripheral antimuscarinic effects of atropine and methylatropine injected systemically and into the cerebral ventricles.

    abstract::We compared the relative abilities of atropine sulfate and methylatropine, injected i.v. and into the cerebral ventricles (icv), to block pharmacological responses mediated through central and peripheral muscarinic receptors. The hypotensive response to i.v. injection of acetylcholine (peripheral muscarinic receptors)...

    journal_title:Life sciences

    pub_type: 杂志文章

    doi:10.1016/0024-3205(88)90389-x

    authors: Brezenoff HE,Xiao YF,Vargas H

    更新日期:1988-01-01 00:00:00

  • The distribution of copper in neonatal mottled mutant mice after exposure to copper and penicillamine.

    abstract::Tissue copper levels of brindled (Mobr) mice and normal litter-mates after single and repeated dosing with CuCl2 and/or D-penicillamine are examined, together with a study of the cytosol distribution of copper after CuCl2 treatment. The results confirm that the mutant mouse kidney is capable of extensive copper accumu...

    journal_title:Life sciences

    pub_type: 杂志文章

    doi:10.1016/0024-3205(82)90326-5

    authors: Hunt DM,Port AE

    更新日期:1982-08-02 00:00:00

  • Dopamine receptor mRNAs in the rat lymphocytes.

    abstract::It has been suggested that dopamine might play a role in the regulation of the immune system. In this study, reverse transcriptase-polymerase chain reaction (RT-PCR) was used to investigate the expression of mRNA for the different subtypes of dopamine receptors in the rat lymphocytes. D1, D3 and D5 receptor mRNAs were...

    journal_title:Life sciences

    pub_type: 杂志文章

    doi:10.1016/s0024-3205(98)00160-x

    authors: Caronti B,Calderaro C,Passarelli F,Palladini G,Pontieri FE

    更新日期:1998-01-01 00:00:00

  • Induction of antioxidant enzymes by dexamethasone in the adult rat lung.

    abstract::Catalase, glutathione peroxidase and superoxide dismutase enzymes were determined after administering dexamethasone. Catalase increased its activity over six times (0.388 U/mg DNA) the normal rate, while glutathione peroxidase caused 3 times an increase one hour after dexamethasone injection. Superoxide dismutase incr...

    journal_title:Life sciences

    pub_type: 杂志文章

    doi:10.1016/s0024-3205(97)00193-8

    authors: José HJ,Berenice SG,Cecilia VR

    更新日期:1997-01-01 00:00:00

  • Mechanisms involved in the possible protective effect of chrysin against sodium arsenite-induced liver toxicity in rats.

    abstract::Arsenic as a one of the most important toxic metals could induce hepatotoxicity. Previous reports revealed the significance of oxidative stress in promoting of arsenic-induced liver toxicity. The aim of the present investigation is to evaluate the effect of chrysin (CHR), a natural flavonoid with potent antioxidant ac...

    journal_title:Life sciences

    pub_type: 杂志文章

    doi:10.1016/j.lfs.2020.118965

    authors: Fatemi I,Khalili H,Mehrzadi S,Basir Z,Malayeri A,Goudarzi M

    更新日期:2021-02-15 00:00:00

  • Discriminative and aversive properties of beta-carboline-3-carboxylic acid ethyl ester, a benzodiazepine receptor inverse agonist, in rhesus monkeys.

    abstract::Rhesus monkeys were trained to discriminate injections of saline from those of beta-carboline-3-carboxylic acid ethyl ester (beta-CCE), a compound that binds to the benzodiazepine receptor, but often has actions opposite to those of the benzodiazepines. A benzodiazepine agonist midazolam and low doses of a specific be...

    journal_title:Life sciences

    pub_type: 杂志文章

    doi:10.1016/0024-3205(86)90240-7

    authors: Takada K,Winger G,Cook J,Larscheid P,Woods JH

    更新日期:1986-03-17 00:00:00