Inhibition of extracellular ATP degradation in endothelial cells.


:The plasma membrane ATPase on the human umbilical vein endothelial cell line (ECV304) was demonstrated to be an ecto-enzyme. Hydrolysis of ATP was measured by monitoring the appearance of inorganic phosphorus. Hydrolysis of extracellular ATP was insensitive to oligomycin, vanadate, ouabain and N-ethylmaleimide, compounds that inhibit the intracellular ion pumping ATPases. Beta-Glycerophosphate (1-10 mM) or p-nitrophenyl phosphate (1-10 mM) did not inhibit hydrolysis of ATP, ruling out the involvement of non-specific phosphatases. Enzyme activity in buffer that had previously been incubated with cells was < 7%, showing that the enzyme activity measured did not result from release of intracellular enzymes. Consistent with this, the cell preparations used were estimated to be > 95% intact as judged by release of cytosolic enzyme lactate dehydrogenase. The enzyme activity was Ca2-/Mg2- dependent. Gramicidin S (20 microM), suramin (100-300 microM), chlorpromazine (250 microM), trifluoperazine (50-250 microM), and thioridazine (100 microM) inhibited the hydrolysis of ATP (3 mM) by 45-80%. The percentage inhibition produced by these substances was not altered in the presence of a concentration of alpha, beta-methylene ADP (10 microM) which inhibited hydrolysis of AMP (3 mM) by 90%, suggesting that these compounds inhibit ecto-ATPase and/or ecto-ADPase. Measurements of absolute amounts of ATP released from various tissues, including the heart, have been hindered because ATP is rapidly and sequentially hydrolysed to adenosine. Identification of compounds that inhibit ATP degradation would prove to be useful to overcome this problem and would lead to the development of invaluable pharmacological tools in many other aspects of purine research.


Life Sci


Life sciences


Meghji P,Burnstock G




Has Abstract


1995-01-01 00:00:00














  • Functional role of nitric oxide in guinea pig tracheal epithelium.

    abstract::Nitric oxide (NO) may play an important regulatory role in airway function. We have, thus, investigated in vitro whether epithelium derived NO may modulate cholinergic neurotransmission, via release of NO in guinea pig trachea, by using L-arginine (L-ARG), a precursor of NO synthesis, and L-N(G)-nitro-arginine-methyl-...

    journal_title:Life sciences

    pub_type: 杂志文章


    authors: Matera MG,D'agostino B,Cardone M,Calderaro V,Rossi F

    更新日期:1995-02-17 00:00:00

  • The role of charge interactions in muscarinic agonist binding, and receptor-response coupling.

    abstract::Site-directed mutagenesis has been used to evaluate the roles of the key aspartate and arginine residues in transmembrane domain three of the muscarinic receptors. The results suggest that the formation of an ionic bond between the Asp carboxylate group and the onium headgroup is essential to anchor acetylcholine in i...

    journal_title:Life sciences

    pub_type: 杂志文章


    authors: Hulme EC,Curtis CA,Page KM,Jones PG

    更新日期:1995-01-01 00:00:00

  • Prostate tumor CXC-chemokine profile correlates with cell adhesion to endothelium and extracellular matrix.

    abstract::Though chemokines of the CXC family are thought to play key roles in neoplastic transformation and tumor invasion, information about CXC chemokines in prostate cancer is sparse. To evaluate the involvement of CXC chemokines in prostate cancer, we analyzed the CXC coding mRNA of both chemokine ligands (CXCL) and chemok...

    journal_title:Life sciences

    pub_type: 杂志文章


    authors: Engl T,Relja B,Blumenberg C,Müller I,Ringel EM,Beecken WD,Jonas D,Blaheta RA

    更新日期:2006-03-13 00:00:00

  • Prostacyclin (PGI2) induces rapid depletion of cyclic AMP levels in brain nuclei of rats.

    abstract::Intravenous injection of prostacyclin (100 micrograms/kg) in rats resulted in a decrease of systolic blood pressure within 2 minutes. Concentrations of cAMP in 15 brain regions and nuclei were determined by radioimmunoassay. In lower brain stem nuclei, such as the nucleus of the solitary tract and the lateral reticula...

    journal_title:Life sciences

    pub_type: 杂志文章


    authors: Schmid G,Bahner U,Palkovits M,Geiger H,Heidland A

    更新日期:1987-12-07 00:00:00

  • Inhibition of gastrointestinal transit by morphine and FK 33-824 in the rat and comparative narcotic antagonist properties of naloxone and its N-methyl quaternary analog.

    abstract::Morphine and the enkephalin-like peptide FK 33-824 given i.p. to rats potently inhibit gastrointestinal transit of a charcoal test meal, the doses inhibiting transit by 50% being respectively about 10 and 1 microgram/kg. These intestinal effects are opiate-specific, locally elicited and presumably involve the same act...

    journal_title:Life sciences

    pub_type: 杂志文章


    authors: Manara L,Bianchi G,Fiocchi R,Notarnicola A,Peracchia F,Tavani A

    更新日期:1982-09-20 00:00:00

  • Distinct subcellular localization of three isoforms of insulinoma-associated protein 2β in neuroendocrine tissues.

    abstract:AIMS:Insulinoma-associated protein 2β (IA-2β) is considered to play a significant role in regulated secretion. Recent studies have shown that the mouse brain expresses three major isoforms of IA-2β, named IA-2β60, IA-2β64, and IA-2β71. In this study, we analyzed the tissue-, cell- and organelle-specific distributions o...

    journal_title:Life sciences

    pub_type: 杂志文章


    authors: Nakajima K,Wu G,Sakudo A,Onodera T,Takeyama N

    更新日期:2011-04-25 00:00:00

  • Autoradiographic localization of [3H]-guanidinoethylmercaptosuccinic acid binding sites in rat islets of Langerhans.

    abstract::Incubation of unfixed, frozen sections of rat pancreas with tritiated guanidinoethylmercaptosuccinic acid (GEMSA) resulted in a localization of [3H]-GEMSA autoradiographic grains over the islets of Langerhans, suggesting that a carboxypeptidase H-like enzyme occurs in the islet. ...

    journal_title:Life sciences

    pub_type: 杂志文章


    authors: Powell AM,Wells MR,Timmers KI,Recant L

    更新日期:1987-06-29 00:00:00

  • Relevance of platelet serotonergic mechanisms in pregnancy induced hypertension.

    abstract::Platelet functions are becoming the useful tool for delineating the etiology of pregnancy induced hypertension. Electronmicroscopic studies, efflux and content of 5-HT in platelets and platelet aggregation responses towards various aggregating agents have been measured in 25 normotensive pregnant subjects and 31 PIH s...

    journal_title:Life sciences

    pub_type: 杂志文章


    authors: Gujrati VR,Goyal A,Gaur SP,Singh N,Shanker K,Chandravati

    更新日期:1994-01-01 00:00:00

  • Renal-thyroid interrelationship in normotensive and hypertensive rats.

    abstract::An increase in the weight of the thyroid gland, accompanied by reductions in uptake and release of 1311, serum T3 and reverse T3 concentrations and an increase in serum TSH concentration, occurs during the development of renal hypertension in rats. Renal function, as assessed by BUN, serum and urinary creatinine conce...

    journal_title:Life sciences

    pub_type: 杂志文章


    authors: Fregly MJ,Threatte RM

    更新日期:1982-02-15 00:00:00

  • Catecholamine systems of retina: a model for studying synaptic mechanisms.

    abstract::The retina contains three catecholamine neurotransmitters: dopamine (DA); norepinephrine (NE); and epinephrine (EPI). DA and EPI appear to be associated with separate amacrine neurons that directly participate in the visual process. NE, in contrast, appears to be associated primarily with the sympathetic nerves that i...

    journal_title:Life sciences

    pub_type: 杂志文章,评审


    authors: Hadjiconstantinou M,Neff NH

    更新日期:1984-09-10 00:00:00

  • Leptin changes Ca2+/calmodulin-dependent response and up-regulates the gene expression of calcineurin in rat hypothalamus.

    abstract::To understand the leptin's action on hypothalamic arcuate nucleus (ARC), Ca2+/calmodulin (CaM)-dependent response in leptin-treated rat ARC was investigated. The in vitro phosphorylation of ARC extracts from leptin-treated rats did not respond to CaM. Although CaM-dependent protein kinase II activity was not affected ...

    journal_title:Life sciences

    pub_type: 杂志文章


    authors: Morishita T,Hidaka T,Sugahara K,Noguchi T

    更新日期:1998-01-01 00:00:00

  • Characterization of homogeneous atrial granule serine proteinase, a candidate processing enzyme of pro-atrial natriuretic factor.

    abstract::We previously reported the discovery and partial characterization of bovine atrial granule serine proteinase, a candidate processing enzyme of pro-atrial natriuretic factor, which is associated with atrial granule membranes. We now report the physicochemical properties of electrophoretically homogeneous enzyme purifie...

    journal_title:Life sciences

    pub_type: 杂志文章


    authors: Wypij DM,Harris RB

    更新日期:1992-01-01 00:00:00

  • Roles of chymase in stenosis occurring after polytetrafluoroethylene graft implantations.

    abstract::Chymase is an important enzyme for the generation of angiotensin (Ang) II and in the activation of transforming growth factor (TGF)-beta1. Therefore, chymase may be involved in the hemodialysis access dysfunction, which is caused by intimal hyperplasia that occurs after polytetrafluoroethylene (PTFE) graft implantatio...

    journal_title:Life sciences

    pub_type: 杂志文章


    authors: Jin D,Ueda H,Takai S,Muramatsu M,Furubayashi K,Ibaraki T,Kishi K,Katsuoka Y,Miyazaki M

    更新日期:2007-09-29 00:00:00

  • Desensitization by cyclic AMP-dependent protein kinase of GABAB receptor expressed in Xenopus oocytes.

    abstract::Involvement of cyclic AMP-dependent protein kinase in the desensitization of GABAB receptor expressed in Xenopus oocytes was studied. GABA produced an outward current in the presence of bicuculline, under voltage-clamp conditions. Baclofen mimicked the effect of GABA, thereby indicating that the response to GABA in th...

    journal_title:Life sciences

    pub_type: 杂志文章


    authors: Yoshimura M,Yoshida S,Taniyama K

    更新日期:1995-11-17 00:00:00

  • Dipeptidyl peptidase IV is a target for covalent adduct formation with the acyl glucuronide metabolite of the anti-inflammatory drug zomepirac.

    abstract::The nonsteroidal anti-inflammatory drug zomepirac (ZP) is metabolised to a chemically reactive acyl glucuronide conjugate (ZAG) which can form covalent adducts with proteins. In vivo, such adducts could initiate immune or toxic responses. In rats given ZP, the major band detected in liver homogenates by immunoblotting...

    journal_title:Life sciences

    pub_type: 杂志文章


    authors: Wang M,Gorrell MD,McGaughan GW,Dickinson RG

    更新日期:2001-01-05 00:00:00

  • Nerve growth factor enhances antigen-specific antibody production in ascorbate-stimulated murine splenocytes.

    abstract::In murine splenocytes, a primary antigen-specific antibody response is stimulated by 2-O-alpha-D-glucopyranosyl-L-ascorbic acid (AA-2G), a stable form of ascorbate, as described in our previous paper. We examined here the effect of nerve growth factor (NGF) on the antigen-specific antibody production in vitro augmente...

    journal_title:Life sciences

    pub_type: 杂志文章


    authors: Yanagida M,Gohda E,Yamamoto I

    更新日期:1996-01-01 00:00:00

  • The neuronal transport mechanism for noradrenaline is not influenced by the ATP analogue ATP gamma S.

    abstract::Noradrenaline is taken up in noradrenergic neurones via a desipramine-sensitive transport system (uptake1). There are reports which describe the activity of uptake1 being increased by extracellular ATP and ATP gamma S. This was attributed to a protein kinase-mediated mechanism. We reevaluated this phenomenon since a m...

    journal_title:Life sciences

    pub_type: 杂志文章


    authors: Gliese M,Babin-Ebell J,Schömig E,Russ H

    更新日期:1994-01-01 00:00:00

  • Effects of asiaticoside on levels of podocyte cytoskeletal proteins and renal slit diaphragm proteins in adriamycin-induced rat nephropathy.

    abstract:AIMS:Centella asiatica has been used to treat kidney diseases in Chinese traditional medicine. Asiaticoside (an extraction of C. asiatica) exerts a variety of pharmacological effects including immunomodulatory and anti-inflammatory functions. However, the mechanism of asiaticoside in the treatment of renal diseases rem...

    journal_title:Life sciences

    pub_type: 杂志文章


    authors: Wang Z,Liu J,Sun W

    更新日期:2013-09-03 00:00:00

  • Changes in responsiveness of the aorta to vasorelaxant agents in genetically diabetic rats: a study in WBN/Kob rats.

    abstract::The effects of various vasorelaxant agents on aortas from control and genetically diabetic rats were examined. The concentration-response curves for the isoproterenol (ISO)-induced relaxation of both aortic strips with and without endothelium are shifted to the right in diabetic rats. The relaxation responses of diabe...

    journal_title:Life sciences

    pub_type: 杂志文章


    authors: Miyata N,Yamaura H,Tsuchida K,Otomo S,Kamata K,Kasuya Y

    更新日期:1992-01-01 00:00:00

  • Calcium-dependence of serotonin-mediated aldosterone secretion and differential effects of calcium antagonists.

    abstract::The requirement for calcium in the serotonin-mediated aldosterone secretion was investigated using rat adrenal capsular cells. In the calcium-free medium both basal as well as serotonin-stimulated aldosterone secretion (at concentrations of 10(-7) M and 10(-8) M of serotonin) were significantly impaired. The effects o...

    journal_title:Life sciences

    pub_type: 杂志文章


    authors: Ganguly A,Hampton T

    更新日期:1985-04-15 00:00:00

  • Intestinal transport of pyridoxine in experimental renal failure.

    abstract::Renal failure (RF) has been shown to alter intestinal transport of a number of nutrients. We studied jejunal absorption of pyridoxine (B6) in rats rendered azotemic by subtotal nephrectomy (RF group) and compared the results with those obtained in normal rats subjected to sham operation (controls) and animals pair-fed...

    journal_title:Life sciences

    pub_type: 杂志文章


    authors: Barbari A,Vaziri ND,Benavides I,Chen YT,Said H,Pahl MV

    更新日期:1989-01-01 00:00:00

  • Chromatographic approaches to signal and selectivity enhancement for determination of biogenic amines by liquid chromatography with amperometric detection.

    abstract::A variety of chromatographic approaches are detailed for enhancement of signal and selectivity for determination of biogenic amines by HPLC with amperometric detection. Microbore HPLC columns offer significant signal enhancement due to the reduced elution volume. Selection of novel surfactants such as N-methyl oleoyl ...

    journal_title:Life sciences

    pub_type: 杂志文章


    authors: Mefford IN

    更新日期:1987-08-17 00:00:00

  • Motilin acts within the CNS to inhibit urinary bladder contractions.

    abstract::Although peripheral actions have been shown for the brain-gut peptide, motilin, its localization in the CNS of mammals suggests some physiological role at this site. In the present experiments intracerebroventricular or intrathecal, but not peripheral, administrations of motilin produced a dose-related and naloxone re...

    journal_title:Life sciences

    pub_type: 杂志文章


    authors: Porreca F,Dray A

    更新日期:1984-06-25 00:00:00

  • Autoradiographic localization of angiotensin II receptors in the rat brainstem.

    abstract::The microscopic localization of angiotensin II receptors in the rat brainstem has been accomplished utilizing in vitro receptor autoradiographic techniques. These receptors are highly localized to discrete nuclear regions of the brainstem. Significant densities of autoradiographic grains, indicating the presence of sp...

    journal_title:Life sciences

    pub_type: 杂志文章


    authors: Gehlert DR,Speth RC,Healy DP,Wamsley JK

    更新日期:1984-04-16 00:00:00

  • [3H]A-69024: a non-benzazepine ligand for in vitro and in vivo studies of dopamine D1 receptors.

    abstract::[3H]A-69024 has been prepared as a radioligand for studying the dopamine D1 receptor. [3H]A-69024 binds to rat striatal membranes with a KD = 14.3 +/- 3.2 nM (mean +/- SEM; n = 3) and Bmax = 63.5 +/- 12.8 fmol/mg wet tissue (1.8 +/- 0.3 pmol/mg protein). This ligand binds to only one site with a Hill coefficient close...

    journal_title:Life sciences

    pub_type: 杂志文章


    authors: Kassiou M,Scheffel UA,Musachio JL,Stathis M,Dannals RF

    更新日期:1995-01-01 00:00:00

  • An acute dose of nicotine does not inhibit MAO B in baboon brain in vivo.

    abstract::Tobacco smoke exposure has been shown to inhibit brain and platelet MAO B in animals and in humans. Though the mechanism(s) responsible for MAO B inhibition are not known, studies in rodents have shown that nicotine administration does not inhibit brain MAO B. In this study we investigated whether brain MAO B is also ...

    journal_title:Life sciences

    pub_type: 杂志文章


    authors: Fowler JS,Volkow ND,Logan J,Pappas N,King P,MacGregor R,Shea C,Garza V,Gatley SJ

    更新日期:1998-01-01 00:00:00

  • Pitavastatin at low dose activates endothelial nitric oxide synthase through PI3K-AKT pathway in endothelial cells.

    abstract::Pitavastatin is a 3-hydroxy-3-methylglutaryl coenzyme A reductase inhibitor that is used for suppressing cholesterol biosynthesis. Previously, we have reported that pitavastatin induces the activation of endothelial nitric oxide synthase (eNOS) and increases nitric oxide (NO) production in vascular endothelial cells (...

    journal_title:Life sciences

    pub_type: 杂志文章


    authors: Wang J,Tokoro T,Matsui K,Higa S,Kitajima I

    更新日期:2005-03-25 00:00:00

  • LEA-1/ICAM-1 interaction is essentially involved in the pathogenesis of delayed-type hypersensitivity-induced liver injury to picryl chloride.

    abstract::The kinetics of lymphocyte function associated antigen 1 (LFA-1) expression on spleen cells (SPC) and liver non-parenchymal cells (NPC), and intercellular adhesion molecule 1 (ICAM-1) expression on hepatocytes (HC) was examined in acute liver injury mice induced by a DTH reaction to picryl chloride (PCl). The peak exp...

    journal_title:Life sciences

    pub_type: 杂志文章


    authors: Xu Q,Jiang J,Cao J,Wu F,Fujii H,Saiki I

    更新日期:1998-01-01 00:00:00

  • Role of alpha 1-adrenoceptor subtypes which mediate positive chronotropy in neonatal rat cardiac myocytes.

    abstract::We investigated the involvement of alpha 1-adrenoceptor subtypes in the positive chronotropic response to norepinephrine (NE) in neonatal rat cardiac myocytes at day 3 of culture. The cardiac myocytes at day 3 of culture exhibited a dose-dependent positive chronotropic response to NE in the presence of propranolol, a ...

    journal_title:Life sciences

    pub_type: 杂志文章


    authors: Kimura H,Kawana S,Kanaya N,Sakano S,Miyamoto A,Ohshika H

    更新日期:1994-01-01 00:00:00

  • Specificity and sensitivity of S100B levels in amniotic fluid for Down syndrome diagnosis.

    abstract::Down syndrome (DS) is the most common chromosomal abnormality and is associated with an extra copy of the chromosome 21. Although several markers are commonly used during pregnancy for the screening of DS, the definitive diagnosis is based on karyotype after amniocentesis, which is an expensive and laborious analysis....

    journal_title:Life sciences

    pub_type: 杂志文章


    authors: Tort AB,Portela LV,da Purificação Tavares M,Gonçalves CA,Netto C,Giugliani R,Souza DO

    更新日期:2004-12-10 00:00:00