Abstract：
:We performed a comparative analysis of biological activity of Lys-Glu peptide and its amino acid constituents. It was established that Lys-Glu stimulated proliferation of splenic cells in organotypic culture, while the mixture of glutamic acid and lysine inhibited culture growth. Using the method of molecular docking, we showed that glutamic acid, lysine, and Lys-Glu peptide can interact with different DNA sequences. The energy of interaction and the most beneficial localization of glutamic acid, lysine, and Lys-Glu peptide in DNA molecule was calculated. We demonstrated the interaction of the peptide and amino acids with DNA along the minor groove. The energy of DNA interaction with the peptide is higher than with individual amino acids. The peptide bonds increase the interaction of Lys-Glu peptide with DNA, which potentiates the biological effect on cell proliferation in organotypic culture of splenic cells.
journal_name
Bull Exp Biol Medjournal_title
Bulletin of experimental biology and medicineauthors
Khavinson VKh,Tarnovskaya SI,Lin'kova NS,Chervyakova NA,Nichik TE,Elashkina EV,Chalisova NIdoi
10.1007/s10517-015-2805-0subject
Has Abstractpub_date
2015-02-01 00:00:00pages
551-4issue
4eissn
0007-4888issn
1573-8221journal_volume
158pub_type
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