Abstract：
:We developed a multiplexed DNA microarray-based assay allowing identification of 12 causative agents of reproductive tract infections with the simultaneous detection of 47 genetic determinants of resistance to antimicrobial substances. The microarray was tested on 93 isolates of Neisseria gonorrhoeae, 32 isolates of Treponema pallidum and 29 samples of Ureaplasma spp./Mycoplasma spp. The N. gonorrhoeae isolates had multiple mutations in the penA, ponA, rpsJ, gyrA, parC, and mtrR genes; their prognostic value significantly increased when combinations of mutations were detected. In the analyzed T. pallidum isolates, single A2058G substitution in the 23S rRNA gene responsible for macrolide resistance was found. DNA sequences of Ureaplasma spp./Mycoplasma spp. were determined as wild type, which was not fully consistent with the results of analysis of their antimicrobial susceptibility.
journal_name
Bull Exp Biol Medjournal_title
Bulletin of experimental biology and medicineauthors
Leinsoo AT,Shaskol'skii BL,Dement'eva EI,Gryadunov DA,Kubanov AA,Chestkov AV,Obraztsova OA,Shpilevaya MV,Deryabin DGdoi
10.1007/s10517-017-3925-5subject
Has Abstractpub_date
2017-11-01 00:00:00pages
54-60issue
1eissn
0007-4888issn
1573-8221pii
10.1007/s10517-017-3925-5journal_volume
164pub_type
杂志文章abstract：:The effects of melatonin, aluminum oxide, and polymethylsiloxane complex on the expression of LYVE-1 (lymphatic vessel endothelial hyaluronan receptor) in the liver were studied in db/db mice with experimental obesity and type 2 diabetes mellitus. The complex or placebo was administered daily by gavage from week 8 to ...
journal_title：Bulletin of experimental biology and medicine
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doi：10.1007/s10517-016-3592-y
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abstract：:We performed a comparative study of the localization, distribution, metabolic activity, and surface properties of human bone marrow mesenchymal stromal cells after static and perfusion seeding to macroporous alginate cryogels. A simple perfusion system for mesenchymal stromal cell seeding to macroporous alginate cryog...
journal_title：Bulletin of experimental biology and medicine
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abstract：:Incomplete varying obstruction of the urinary tract was reproduced by injecting artificial stomatological material into the rat bladder. Inflammatory changes and nephrosclerosis were detected in the renal tissue on days 14 and 21 of the experiment. Urinary concentration of total protein and activity of γ-glutamylamino...
journal_title：Bulletin of experimental biology and medicine
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doi：10.1007/s10517-013-2062-z
更新日期：2013-04-01 00:00:00
abstract：:Effects of local therapy with miacalcic on the metabolism in articular connective tissue structures under conditions of experimental arthritis are shown. An effect modifying articular tissue metabolism and an antiinflammatory effect preventing the development of inflammatory destructive processes in rabbit articular t...
journal_title：Bulletin of experimental biology and medicine
pub_type： 杂志文章
doi：10.1007/s10517-007-0108-9
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abstract：:Arginine-vasopressin or arginine-vasotocin hormones introduced into the small intestine are absorbed and exert a hydrosmotic effect. The amount of absorbed antidiuretic hormone increased when it is introduced with intestinal peptidase inhibitor aprotinin. These data attest to the possibility of absorption of pituitary...
journal_title：Bulletin of experimental biology and medicine
pub_type： 杂志文章
doi：10.1023/b:bebm.0000024370.53746.ac
更新日期：2004-01-01 00:00:00
abstract：:The effects of Biopin (ointment containing pine resin) used in the treatment of burns, wounds, and pyoinflammatory processes on cell composition of the wound surface (smears) and granulation tissue were studied on rats. In vitro antibacterial activity of the drug was also evaluated. At the early stage of inflammation ...
journal_title：Bulletin of experimental biology and medicine
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abstract：:The time course of angiotensin-converting enzyme activity in the rat aorta after fractionated exposure to ionizing radiation and the effects of dihydroquercetin and fucoidin on this parameter were studied. Male Wistar rats were exposed to single or repeated (fractionated) X-ray radiation in a dose of 2.5 Gy at 200 kV....
journal_title：Bulletin of experimental biology and medicine
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更新日期：2018-07-01 00:00:00
abstract：:Time course of DNase and RNase activities in the sera of random-bred mice infected with Plasmodium malariae (chloroquine-sensitive strain H) was studied. Similarly to viral infection, infection with a protozoa eukaryotic parasite was accompanied by increased DNAse and RNAse activities, though Plasmodium malariae biolo...
journal_title：Bulletin of experimental biology and medicine
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doi：
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journal_title：Bulletin of experimental biology and medicine
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abstract：:The effect of Dicarbamin preparation on hemopoiesis suppressed with cyclophosphamide was studied in animal experiments. It was shown that Dicarbamin produced a protective effect on granulocytic hemopoietic steam. This property of the preparation is determined by both protection of immature granulocytic cells at early ...
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journal_title：Bulletin of experimental biology and medicine
pub_type： 杂志文章,已发布勘误
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更新日期：2017-09-01 00:00:00
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journal_title：Bulletin of experimental biology and medicine
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更新日期：2002-07-01 00:00:00
abstract：:Addition of anaferon (pediatric formulation) to the therapy of chickenpox patients led to more rapid disappearance of the main symptoms and alleviated the course of the disease. The safety of the preparation is confirmed by the absence of undesirable events and stability of laboratory indexes against the background of...
journal_title：Bulletin of experimental biology and medicine
pub_type： 杂志文章,随机对照试验
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更新日期：2009-08-01 00:00:00
abstract：:Delivery of plasmid DNA and interfering RNA into adipose tissue stromal cells was carried out by the methods of lipofection, calcium phosphate method, and by electroporation. The percent of transfected cells after delivery of plasmid DNA by the calcium phosphate method and lipofection was 0 and 15%, respectively, vs. ...
journal_title：Bulletin of experimental biology and medicine
pub_type： 杂志文章
doi：10.1007/s10517-009-0546-7
更新日期：2009-04-01 00:00:00
abstract：:The appearance of autoantibodies to neuronal proteins (S100, GFAP, MBP, and NGF) in rat serum was analyzed by ELISA on days 5, 10, 17, 25, and 32 after streptozotocin injection. Simultaneously, blood glucose and insulin autoantibodies were assayed. Serum glucose level increased on the next day after streptozotocin inj...
journal_title：Bulletin of experimental biology and medicine
pub_type： 杂志文章
doi：10.1007/s10517-013-2077-5
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journal_title：Bulletin of experimental biology and medicine
pub_type： 杂志文章
doi：10.1023/a:1013174802845
更新日期：2001-09-01 00:00:00
abstract：:Angiogenic action of compound GK-2, a dimeric dipeptide mimetic of loop 4 of nerve growth factor (NGF), was studied in in vitro and in vivo experiments. Experiments on human endothelial cell culture HUVEC showed that compound GK-2 significantly (p<0.05) stimulated the initial stage of angiogenesis, and its angiogenic ...
journal_title：Bulletin of experimental biology and medicine
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doi：10.1007/s10517-016-3450-y
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abstract：:Apoptosis of blood neutrophils from healthy donors was studied under conditions of cell culturing with different concentrations of H(2)O(2), selective NO synthase inhibitor, and inductor of NO synthesis (L-arginine). In vitro incubation of neutrophilic leukocytes with 5 mM H(2)O(2) led to activation of the apoptotic p...
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abstract：:We studied the effect of allotransplantation of bone marrow mononuclear cells on postinfarction remodeling of the heart in rats. The cells were transplanted into the periinfarction zone of the heart. The transplantation was performed on day 9 after coronary occlusion. It was found that on day 45 after coronary occlusi...
journal_title：Bulletin of experimental biology and medicine
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更新日期：2008-09-01 00:00:00
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abstract：:An integrative systemic model of pharmacological reaction to a phytopreparation was created as a result of conceptual structurization of facts and formalization of causal relations. The use of the new methodological approach to the choice, primary screening, and profound studies of plants for prospective addition ther...
journal_title：Bulletin of experimental biology and medicine
pub_type： 杂志文章
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abstract：:The antiamnestic effects of tenoten (pediatric formulation) was demonstrated on the model of scopolamine-induced amnesia of passive avoidance reflex and the nootropic effect of this preparation was demonstrated on the model of incomplete conditioning and in rat pups with experimental attention deficit syndrome. The ef...
journal_title：Bulletin of experimental biology and medicine
pub_type： 杂志文章
doi：10.1007/s10517-010-0754-1
更新日期：2009-09-01 00:00:00
abstract：:Study on a model of 6-day dosed adaptation to heat in rats showed that this adaptation decreased the severity of cardiac arrhythmias during ischemic and reperfusion injury. The duration of arrhythmias decreased not only in the ischemic period, but also under conditions of reperfusion. Adaptation delayed the developmen...
journal_title：Bulletin of experimental biology and medicine
pub_type： 杂志文章
doi：10.1007/s10517-007-0003-4
更新日期：2007-01-01 00:00:00
abstract：:Local injection of 4 nmol moxonidine (unilaterally) into the rostroventrolateral medulla of spontaneously hypertensive rats (SHR-SP) decreased mean blood pressure and heart rate by 24+/-3 and 3+/-4%, respectively. Pretreatment with the I1/alpha2-receptor antagonist efaroxan abolished the moxonidine-induced decrease in...
journal_title：Bulletin of experimental biology and medicine
pub_type： 杂志文章
doi：10.1023/a:1017943901070
更新日期：2001-04-01 00:00:00
abstract：:We studied biocompatibility and bioresorption of 3D-printed polylactide and polyglycolide tissue membranes. Ultrasound microscopy and histological examination showed that membranes fabricated of a copolymer of lactic and glycolic acids in a mass ratio of 1:9 are bioresorbed and have good biocompatibility with soft tis...
journal_title：Bulletin of experimental biology and medicine
pub_type： 杂志文章
doi：10.1007/s10517-021-05066-x
更新日期：2021-01-01 00:00:00
abstract：:Plasma LDL content increased in rats with mild chronic heart failure, but decreased in animals with severe pathology. The lipid profile of β-adrenoceptor antagonists depended on their selectivity. The most selective antagonists of β-adrenoceptors had little effect on the lipid composition. Nonselective β-adrenoceptor ...
journal_title：Bulletin of experimental biology and medicine
pub_type： 杂志文章
doi：10.1007/s10517-013-2312-0
更新日期：2013-12-01 00:00:00
