Abstract:
:We proposed a new indicator for evaluation of functional activity of Ca(2+) in human blood serum based on lysis of sheep erythrocytes with 10% human blood serum in the presence of 0.55 mM ethylene glycol tetraacetic acid at 37°C for 10 min. After incubation, the degree of sheep erythrocytes lysis inhibition is estimated: inhibition of complement hemolytic activity <30% is considered as high functional Са(2+) activity, inhibition by 31-70% corresponds to normal activity, and >71% indicates low activity. The comparative studies of complement activating function of heterophilic antibodies, complement system reactivity in the presence of 0.29 M NaCl, and functional activity of ionized Ca(2+) make possible estimation of the individual's immune status.
journal_name
Bull Exp Biol Medjournal_title
Bulletin of experimental biology and medicineauthors
Shoibonov BB,Grigor'eva DV,Shabalina AA,Kostyreva MV,Baronets VY,Zamolodchikova TSdoi
10.1007/s10517-015-2948-zsubject
Has Abstractpub_date
2015-07-01 00:00:00pages
305-8issue
3eissn
0007-4888issn
1573-8221journal_volume
159pub_type
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