Abstract：
:Delivery of plasmid DNA and interfering RNA into adipose tissue stromal cells was carried out by the methods of lipofection, calcium phosphate method, and by electroporation. The percent of transfected cells after delivery of plasmid DNA by the calcium phosphate method and lipofection was 0 and 15%, respectively, vs. more than 50% after electroporation. Similar results were obtained for delivery of short-strand RNA into cells. These data indicate that electroporation is the most effective method of nonviral transfection of adipose tissue stromal cells.
journal_name
Bull Exp Biol Medjournal_title
Bulletin of experimental biology and medicineauthors
Lopatina TV,Kalinina NI,Parfyonova EVdoi
10.1007/s10517-009-0546-7subject
Has Abstractpub_date
2009-04-01 00:00:00pages
509-12issue
4eissn
0007-4888issn
1573-8221journal_volume
147pub_type
杂志文章abstract：:Digital simulation of disorders in the p53 and Mdm2 protein system, involved in repair of DNA defects and in many vital processes, including tumor formation, neurodegeneration, and aging, was carried out on the base of functional nonlinear octoparametric mathematical model. Stress situations were studied, associated w...
journal_title：Bulletin of experimental biology and medicine
pub_type： 杂志文章
doi：10.1007/s10517-014-2608-8
更新日期：2014-08-01 00:00:00
abstract：:Rat liver was examined by transmission electron microscopy after a single intravenous injection of nanosized magnetite suspension (0.1 g (Fe(3)O(4))/kg body weight). Magnetite particles were found in Kupffer's cells and hepatocytes. Accumulation of the particles by these two cell types was different. Morphometry of ma...
journal_title：Bulletin of experimental biology and medicine
pub_type： 杂志文章
doi：10.1007/s10517-012-1756-y
更新日期：2012-08-01 00:00:00
abstract：:The development of experimental type II diabetes mellitus in rats was accompanied by dysfunction of inhibitory and stimulatory heterotrimeric G-proteins, components of hormone-sensitive adenylate cyclase signal system. The function of inhibitory G-proteins decreased most significantly under these conditions, which is ...
journal_title：Bulletin of experimental biology and medicine
pub_type： 杂志文章
doi：10.1007/s10517-006-0451-2
更新日期：2006-12-01 00:00:00
abstract：:We studied the role of insulin-like growth factors 1 and 2 (IGF-1 and IGF-2) in functional differentiation of HC11 mouse mammary gland cells. It was found that both IGF-1 and IGF-2 activate the expression of milk protein β-casein in the presence of prolactin and hydrocortisone. It was found that β-casein expression is...
journal_title：Bulletin of experimental biology and medicine
pub_type： 杂志文章
doi：10.1007/s10517-011-1488-4
更新日期：2011-12-01 00:00:00
abstract：:We studied the formation and cytophysiological characteristics of polynuclear macrophages in primary cultures of peritoneal cells from C57Bl/6 mice. Production of reactive oxygen species and phagocytic activity in polynuclear macrophages were higher than in mononuclear macrophages. The formation of polynuclear macroph...
journal_title：Bulletin of experimental biology and medicine
pub_type： 杂志文章
doi：10.1007/s10517-009-0405-6
更新日期：2008-12-01 00:00:00
abstract：:The rate and strength of heart contractions decreased after 10-min perfusion of rat myocardium with Krebs-Henseleit solution containing a selective cannabinoid receptor agonist HU-210 in a final concentration of 10 nM. HU-210 completely blocked the positive inotropic and chronotropic effect of beta-adrenoceptor agonis...
journal_title：Bulletin of experimental biology and medicine
pub_type： 杂志文章
doi：10.1007/s10517-005-0123-7
更新日期：2004-12-01 00:00:00
abstract：:We studied etiologic structure of bacterial intestinal infections in monkeys of Adler nursery. A total of 533 monkeys with diarrhea syndrome and monkeys dead from intestinal infections, as well as clinically healthy monkeys and animals dead from other pathologies were examined by bacteriological and molecular-genetic ...
journal_title：Bulletin of experimental biology and medicine
pub_type： 杂志文章
doi：10.1007/s10517-011-1428-3
更新日期：2011-10-01 00:00:00
abstract：:Effect of the dipeptidyl peptidase-4 inhibitor linagliptin on structural manifestations of diabetic nephropathy was studied in BKS.Cg-Dock7m+/+Leprdb/J mice (experimental model of type 2 diabetes mellitus). Linagliptin (10 mg/kg per day) or vehicle was administered by gavage over 8 weeks. Mesangial expansion, thickeni...
journal_title：Bulletin of experimental biology and medicine
pub_type： 杂志文章
doi：10.1007/s10517-016-3447-6
更新日期：2016-08-01 00:00:00
abstract：:Calmodulin inhibitor W-7 did not cause changes in the quantal content of postsynaptic end-plate potentials (EPP) in newly formed synapses, but prevented facilitation of acetylcholine secretion induced by L-type Ca(2+)channels blocker nitrendipine. CaMKII inhibitor KN-62 produced similar effect and suppressed the incre...
journal_title：Bulletin of experimental biology and medicine
pub_type： 杂志文章
doi：10.1007/s10517-013-1940-8
更新日期：2013-01-01 00:00:00
abstract：:We studied the effects of dipeptidyl peptidase 4 (DPP4) inhibitor linagliptin on the expression of apoptosis regulator proteins Bcl-2 and Bad in the liver of db/db mice with genetically determined obesity and type 2 diabetes mellitus. The mice received daily linagliptin or saline (placebo) by gavage from week 10 to we...
journal_title：Bulletin of experimental biology and medicine
pub_type： 杂志文章
doi：10.1007/s10517-019-04493-1
更新日期：2019-06-01 00:00:00
abstract：:Antiaggregant activity of a new tricyclic benzimidazole derivative, RU-891 compound, was studied on the model of ADP-induced platelet aggregation in vitro and intravascular platelet aggregation in vivo. We evaluated the effect of this substance on blood coagulation potential. Antiaggregant agent acetylsalicylic acid w...
journal_title：Bulletin of experimental biology and medicine
pub_type： 杂志文章
doi：10.1007/s10517-014-2453-9
更新日期：2014-04-01 00:00:00
abstract：:The effect of epithalamin on circadian rhythms of thymic serum factor titers and melatonin concentrations in the blood of elderly people was studied. Course treatment with epithalamin modulated the rhythm of the thymic endocrine function. The increase in the titer of thymic serum factor at night coincided with the inc...
journal_title：Bulletin of experimental biology and medicine
pub_type： 杂志文章
doi：10.1023/b:bebm.0000038165.09563.b5
更新日期：2004-05-01 00:00:00
abstract：:We studied erythropoiesis in newborn infants delivered by mothers with normal pregnancy and gestosis. The effects of placental extracts on hemopoiesis in JCR mice were also evaluated. Our results suggest that the placenta is involved in the regulation of fetal erythropoiesis. The placenta activates fetal erythropoiesi...
journal_title：Bulletin of experimental biology and medicine
pub_type： 杂志文章
doi：10.1023/a:1017908322856
更新日期：2001-11-01 00:00:00
abstract：:The morphology of neural and glial cells in different cerebral areas and in various experimental models was quantitatively studied by methods of computer morphometry combined with video image analysis systems. The following parameters were obtained: density of neurons and glial cells per 1 mm(2), number of satellite p...
journal_title：Bulletin of experimental biology and medicine
pub_type： 杂志文章
doi：10.1007/s10517-010-0886-3
更新日期：2010-07-01 00:00:00
abstract：:Time course of DNase and RNase activities in the sera of random-bred mice infected with Plasmodium malariae (chloroquine-sensitive strain H) was studied. Similarly to viral infection, infection with a protozoa eukaryotic parasite was accompanied by increased DNAse and RNAse activities, though Plasmodium malariae biolo...
journal_title：Bulletin of experimental biology and medicine
pub_type： 杂志文章
doi：
更新日期：2000-09-01 00:00:00
abstract：:New methods of restriction fragment length polymorphism analysis were developed to distinguish polymorphic variants C190T, G191A, G857A, and 859Del of the NAT2 gene located close to each other. ...
journal_title：Bulletin of experimental biology and medicine
pub_type： 杂志文章
doi：10.1007/s10517-009-0309-5
更新日期：2008-10-01 00:00:00
abstract：:Initiation of apoptosis by chemotherapeutic drugs is one of the most effective approaches to the treatment of cancers. Caspases, the main enzymes of apoptosis, undergo activation to initiate cell death. Activation of initiator caspases requires their binding to special protein complexes. For elucidation of the mechani...
journal_title：Bulletin of experimental biology and medicine
pub_type： 杂志文章
doi：10.1007/s10517-019-04663-1
更新日期：2019-11-01 00:00:00
abstract：:The mechanisms underlying cardioprotective activity of compound ALM-802 were studied in experiments on rats with chronic post-infarction heart failure. Real-time PCR showed that compound ALM-802 (daily intraperitoneal injections in a dose of 2 mg/kg for 28 days starting from day 91 after myocardial infarction modeling...
journal_title：Bulletin of experimental biology and medicine
pub_type： 杂志文章
doi：10.1007/s10517-021-05058-x
更新日期：2021-01-01 00:00:00
abstract：:We studied the mechanisms of combined action of clonidine and local anesthetics amethocaine and imidazo-benzimidazole derivative RU-1117. In contrast to amethocaine, RU-1117 in therapeutic concentrations binds to imidazoline receptors and, to a lesser extent, to alpha(2A)-adrenoceptors on human platelets. Clonidine an...
journal_title：Bulletin of experimental biology and medicine
pub_type： 杂志文章
doi：10.1023/a:1026088813307
更新日期：2003-07-01 00:00:00
abstract：:Non-clonal and clonal abnormal cells (hypodiploid, polyploid, and with chromosome aberrations) were detected by the G-method in the bone marrow and blood of the majority of patients with non-Hodgkin lymphomas before therapy. The manifestation of these abnormalities changed individually after treatment. The most incide...
journal_title：Bulletin of experimental biology and medicine
pub_type： 杂志文章
doi：10.1007/s10517-007-0059-1
更新日期：2007-02-01 00:00:00
abstract：:The relative content of myocyte-active factors and endogenous muscarinic receptor blocker in the blood increased, while the concentration of endogenous beta-adrenoceptor sensitizer decreased in coronary patients with a history of acute myocardial infarction. Physical training produced a therapeutic effect, normalized ...
journal_title：Bulletin of experimental biology and medicine
pub_type： 杂志文章
doi：10.1023/a:1026068208764
更新日期：2003-07-01 00:00:00
abstract：:Complex clinical, dental, and morphological investigation, and ELISA of levels of MMP-2, 8, 9, and TIMP-1 and 2 in the saliva was performed during primary examination of patients with premalignant lesions of maxillofacial area and practically healthy volunteers. Levels of all study MMP in the saliva significantly diff...
journal_title：Bulletin of experimental biology and medicine
pub_type： 杂志文章
doi：10.1007/s10517-017-3744-8
更新日期：2017-05-01 00:00:00
abstract：:Therapy of rats with CCl4 hepatitis with Stellaria media L. water-soluble polysaccharide fraction in a dose of 100 mg/kg reduces serum activities of transaminases (ALT and AST), alkaline phosphatase, bilirubin, and the thymol test values. In the liver, the density of inflammatory infiltration of the organ parenchyma, ...
journal_title：Bulletin of experimental biology and medicine
pub_type： 杂志文章
doi：10.1007/s10517-013-2021-8
更新日期：2013-03-01 00:00:00
abstract：:The relationship between dromotropic and chronotropic components of various cardiac reflexes was studied in cats. Intravenous infusion of blood was mainly accompanied by unidirectional negative chronotropic and dromotropic effects, but the dynamics of these effects was different. Clumping of the carotid arteries in mo...
journal_title：Bulletin of experimental biology and medicine
pub_type： 杂志文章
doi：10.1007/s10517-008-0041-6
更新日期：2008-02-01 00:00:00
abstract：:High doses of phenylephrine and diazepam (1 and 10 mg/kg, respectively) suppressed the development of generalized tonic-clonic pentylenetetrazole-induced convulsions in 86-100% rats, but did not prevent local clonic pentylenetetrazole-induced convulsions. Diazepam in the specified dose produced strong sedation, while ...
journal_title：Bulletin of experimental biology and medicine
pub_type： 杂志文章
doi：10.1007/s10517-014-2730-7
更新日期：2014-12-01 00:00:00
abstract：:The effect of p-tyrosol on the spontaneous level of DNA damage in the cells of the bone marrow, liver, kidney, and rectum of mice (series I) and on the genotoxic effects of cytostatic drugs with different mechanisms of action in rat testicular cells (series II) was studied by DNA comet assay on C57BL/6 mice. p-Tyrosol...
journal_title：Bulletin of experimental biology and medicine
pub_type： 杂志文章
doi：10.1007/s10517-020-04857-y
更新日期：2020-06-01 00:00:00
abstract：:Inductors of the monooxygenase system benzonal, halonal, and halodif prevented the development of intrahepatic cholestasis induced by a-naphthylisothiocyanate and stimulated detoxifying function of the liver in rats. These agents increased the content of microsomal protein and cytochrome P-450 and accelerated metaboli...
journal_title：Bulletin of experimental biology and medicine
pub_type： 杂志文章
doi：10.1023/a:1021563703378
更新日期：2002-09-01 00:00:00
abstract：:Crystal aggregations of oral fluid from normal subjects and patients with type 2 diabetes mellitus were examined. Morphological signs characterizing crystal aggregations of salivary pools from patients with type 2 diabetes mellitus are described and classified. ...
journal_title：Bulletin of experimental biology and medicine
pub_type： 杂志文章
doi：10.1007/s10517-005-0027-6
更新日期：2004-09-01 00:00:00
abstract：:T cells were most sensitive to cyclophosphamide in DBA/2 mice, while in C57Bl/6 mice both T and B cells were sensitive. The formation of antibody-producing cells and the production of specific antibodies were delayed in DBA/2 mice immunized after pretreatment with antitumor drug. Accumulation of antibody-producing cel...
journal_title：Bulletin of experimental biology and medicine
pub_type： 杂志文章
doi：10.1023/a:1024664815450
更新日期：2003-04-01 00:00:00
abstract：:We studied the effect of audiovisual stimulation training course on physical development, functional state of the cardiovascular system, blood biochemical parameters, and hormonal status of athletes. The training course led to improvement of physical performance and adaptive capacities of the circulatory system, incre...
journal_title：Bulletin of experimental biology and medicine
pub_type： 杂志文章
doi：10.1007/s10517-016-3474-3
更新日期：2016-09-01 00:00:00
