Abstract：
:Initiation of apoptosis by chemotherapeutic drugs is one of the most effective approaches to the treatment of cancers. Caspases, the main enzymes of apoptosis, undergo activation to initiate cell death. Activation of initiator caspases requires their binding to special protein complexes. For elucidation of the mechanisms of apoptosis, these complexes should be isolated. However, their purification is challenging because they are formed in the cell in negligible amounts and rapidly degrade. We have developed an effective way to isolate caspase activation complexes formed in tumor cells in response to DNA damage. The method is based on combination of gel filtration with immunoprecipitation. The first stage is aimed at the separation of the high-molecular-weight caspase activation complexes and their monomeric forms, which allows increasing the efficiency of isolation of complexes at the second stage.
journal_name
Bull Exp Biol Medjournal_title
Bulletin of experimental biology and medicineauthors
Zamaraev AV,Egorshina AY,Lavrik IN,Zhivotovsky BD,Kopeina GSdoi
10.1007/s10517-019-04663-1subject
Has Abstractpub_date
2019-11-01 00:00:00pages
132-140issue
1eissn
0007-4888issn
1573-8221pii
10.1007/s10517-019-04663-1journal_volume
168pub_type
杂志文章abstract：:We studied ultrastructural reorganization of the colon transplant in delayed period after esophagoplasty. It was found that during functioning of the artificial esophagus, a complex of adaptive and pathologic processes occurs in the mucosa. Focal sclerosis of the mucosa and slight epithelial degeneration with hyperpla...
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abstract：:The second author's name should read A. Yu. Romanov. ...
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