Diagnosis of nitrosative stress by quantitative EPR-spectroscopy of epidermal cells.

Abstract:

:Quantitative assay of nitric oxide (NO) and iron (Fe) was carried out by the electron paramagnetic resonance (EPR) method in epidermal derivative (hair) of 45 liquidators of the Chernobyl accident, 30 employees of radiological institutions having contact with radiation, and 50 students of Vladimir State University, most of which were diagnosed with iron deficiency, namely iron deficiency anemia (4 persons) and latent iron deficiency (34 persons). Measurements were performed on a Radiopan EPR-spectrometer. It was found that the method of quantitative EPR-spectroscopy using diethyldithiocarbamate as a trap in vitro could be successfully used to measure NO-radical activity in such a biological substrate as hair. It was found that the intensity of NO-radical signal in the spectrogram depends on the Fe level (not only in the analyzed substrate, but also in the whole organism).

journal_name

Bull Exp Biol Med

authors

Petukhov VI,Baumane LK,Reste ED,Zvagule TY,Romanova MA,Shushkevich NI,Sushkova LT,Skavronsky SV,Shchukov AN

doi

10.1007/s10517-013-2042-3

subject

Has Abstract

pub_date

2013-04-01 00:00:00

pages

734-6

issue

6

eissn

0007-4888

issn

1573-8221

journal_volume

154

pub_type

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