Mesenchymal stem cells from human bone marrow and adipose tissue: isolation, characterization, and differentiation potentialities.

Abstract:

:Comparative study of cultured human bone marrow and adipose tissue (lipoaspirate) mesenchymal stem cells was carried out. The main morphological parameters, proliferative activity, expression of surface and intracellular markers of these cells were characterized. Flow cytofluorometry and histological staining showed that both cell types exhibited similar expression of CD105, CD54, CD106, HLA-I markers, were positively stained for vimentin, ASMA, collagen-1, and fibronectin, but not HLA-DR, CD117, and hemopoietic cell markers. The cells underwent differentiation into adipocytes and osteoblasts under appropriate conditions of culturing. Incubation under neuroinductive conditions led to the appearance of a cell population positively stained for type III beta-tubulin (neuronal differentiation marker).

journal_name

Bull Exp Biol Med

authors

Romanov YA,Darevskaya AN,Merzlikina NV,Buravkova LB

doi

10.1007/s10517-005-0430-z

subject

Has Abstract

pub_date

2005-07-01 00:00:00

pages

138-43

issue

1

eissn

0007-4888

issn

1573-8221

pii

doi

journal_volume

140

pub_type

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