Abstract:
:Comparative study of cultured human bone marrow and adipose tissue (lipoaspirate) mesenchymal stem cells was carried out. The main morphological parameters, proliferative activity, expression of surface and intracellular markers of these cells were characterized. Flow cytofluorometry and histological staining showed that both cell types exhibited similar expression of CD105, CD54, CD106, HLA-I markers, were positively stained for vimentin, ASMA, collagen-1, and fibronectin, but not HLA-DR, CD117, and hemopoietic cell markers. The cells underwent differentiation into adipocytes and osteoblasts under appropriate conditions of culturing. Incubation under neuroinductive conditions led to the appearance of a cell population positively stained for type III beta-tubulin (neuronal differentiation marker).
journal_name
Bull Exp Biol Medjournal_title
Bulletin of experimental biology and medicineauthors
Romanov YA,Darevskaya AN,Merzlikina NV,Buravkova LBdoi
10.1007/s10517-005-0430-zsubject
Has Abstractpub_date
2005-07-01 00:00:00pages
138-43issue
1eissn
0007-4888issn
1573-8221pii
doijournal_volume
140pub_type
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