Abstract:
BACKGROUND:OCT3/4 (POU5F1) is an established diagnostic immunohistochemical marker for specific histological variants of human malignant germ cell tumours (GCTs), including the seminomatous types and the stem cell component of non-seminomas, known as embryonal carcinoma. OCT3/4 is crucial for the regulation of pluripotency and the self-renewal of normal embryonic stem- and germ cells. Detection of expression of this transcription factor is complicated by the existence of multiple pseudogenes and isoforms. Various claims have been made about OCT3/4 expression in non-GCTs, possibly related to using nonspecific detection methods. False-positive findings undermine the applicability of OCT3/4 as a specific diagnostic tool in a clinical setting. In addition, false-positive findings could result in misinterpretation of pluripotency regulation in solid somatic cancers and their stem cells. Of the three identified isoforms--OCT4A, OCT4B and OCT4B1--only OCT4A proved to regulate pluripotency. Up until now, no convincing nuclear OCT4A protein expression has been shown in somatic cancers or tissues. METHODS:This study investigates expression of the various OCT3/4 isoforms in GCTs (both differentiated and undifferentiated) and somatic (non-germ cell) cancers, including representative cell lines and xenografts. RESULTS:Using specific methods, OCT4A and OCT4B1 are shown to be preferentially expressed in undifferentiated GCTs. The OCT4B variant shows no difference in expression between GCTs (either differentiated or undifferentiated) and somatic cancers. In spite of the presence of OCT4A mRNA in somatic cancer-derived cell lines, no OCT3/4 protein is detected. Significant positive correlations between all isoforms of OCT3/4 were identified in both tumours with and without a known stem cell component, possibly indicating synergistic roles of these isoforms. CONCLUSION:This study confirms that OCT4A protein only appears in seminomatous GCTs, embryonal carcinoma and representative cell lines. Furthermore, it emphasises that in order to correctly assess the presence of functional OCT3/4, both isoform specific mRNA and protein detection are required.
journal_name
Br J Cancerjournal_title
British journal of cancerauthors
Rijlaarsdam MA,van Herk HA,Gillis AJ,Stoop H,Jenster G,Martens J,van Leenders GJ,Dinjens W,Hoogland AM,Timmermans M,Looijenga LHdoi
10.1038/bjc.2011.270subject
Has Abstractpub_date
2011-09-06 00:00:00pages
854-63issue
6eissn
0007-0920issn
1532-1827pii
bjc2011270journal_volume
105pub_type
杂志文章abstract::A prospective, consecutive study of the aetiology of treatment-associated diarrhoea was conducted in 25 patients with disseminated germ cell cancer treated with intensive chemotherapy. Clostridium difficile was isolated in 45% of the diarrhoea episodes, which makes this species the most important bacterial pathogen in...
journal_title:British journal of cancer
pub_type: 杂志文章
doi:10.1038/bjc.1992.334
更新日期:1992-10-01 00:00:00
abstract::The vascular architecture of four different tumour cell lines (CaX, CaNT, SaS, HEC-1B) transplanted subcutaneously in mice was examined by means of microvascular corrosion casting in order to determine whether there is a characteristic vascular pattern for different tumour types and whether it differs significantly fr...
journal_title:British journal of cancer
pub_type: 杂志文章
doi:10.1038/sj.bjc.6690416
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abstract::The purpose of this study was to compare the growth-inhibitory effect of cisplatin-paclitaxel with that obtained with a cisplatin-docetaxel combination and to assess the type of interaction. Concomitant use of taxanes and cisplatin was studied in seven human ovarian carcinoma cell lines, using the 96-well plate clonog...
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doi:10.1038/sj.bjc.6690046
更新日期:1999-01-01 00:00:00
abstract::Nuclear polyploidization in the livers of CF-1 mice, exposed to dieldrin (0, 1, 5 and 10 ppm in the diet), was studied up to the median time of liver tumour development (ranging from 15 to 27 months) in the respective treatment groups. In untreated controls nuclear polyploidization is characterized by a linear increas...
journal_title:British journal of cancer
pub_type: 杂志文章
doi:10.1038/bjc.1988.160
更新日期:1988-07-01 00:00:00
abstract::In haemagglutination tests we have found that the monoclonal antibody B5 discriminates between erythrocytes from patients with malignant cancer (total 386; greater than 80% B5 positive) and individuals with no known tumour (total 247; less than 20% B5 positive). The B5 antigen detected on intact erythrocytes is a tigh...
journal_title:British journal of cancer
pub_type: 杂志文章
doi:10.1038/bjc.1984.53
更新日期:1984-03-01 00:00:00
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pub_type: 杂志文章
doi:10.1038/bjc.1997.472
更新日期:1997-01-01 00:00:00
abstract::Relationships between incidence of Wilms' tumour and information recorded at birth were investigated in a prospective study of the 1,489,297 children born in Norway between 1967 and 1992. A total of 119 individuals were diagnosed with Wilms' tumour in the age interval 0-14 years. A high length at birth was significant...
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pub_type: 杂志文章
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更新日期:1996-10-01 00:00:00
abstract::Geographical correlations between standardized, mortality ratios (SMRs) of cancers and consumption of different types of alcoholic beverages (saké synthetic saké, shochu, beer, wine, and whisky), of cigarettes, and urbanization were examined for all 46 prefectures in Japan. Suggestive correlations were observed betwee...
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pub_type: 杂志文章
doi:10.1038/bjc.1979.200
更新日期:1979-09-01 00:00:00
abstract::The aim of the study was to evaluate efficacy and tolerance of epirubicin and gemcitabine as first-line chemotherapy in patients with advanced non-small-cell lung cancer. A phase I study was performed with the combination of escalating doses of epirubicin intravenously on day 1 and a fixed dose of gemcitabine on days ...
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pub_type: 临床试验,杂志文章,多中心研究
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更新日期:2000-02-01 00:00:00
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journal_title:British journal of cancer
pub_type: 杂志文章
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journal_title:British journal of cancer
pub_type: 杂志文章
doi:10.1038/bjc.1991.56
更新日期:1991-02-01 00:00:00
abstract:BACKGROUND:Tallness has consistently been associated with an increased risk of breast cancer. We investigated the association further by decomposing height into leg length and sitting height. METHODS:From the prospective Danish cohort 'Diet, Cancer and Health', 23 864 postmenopausal women enrolled during 1993-1997 wer...
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pub_type: 杂志文章
doi:10.1038/bjc.2012.244
更新日期:2012-06-26 00:00:00
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journal_title:British journal of cancer
pub_type: 杂志文章
doi:10.1038/bjc.1992.7
更新日期:1992-01-01 00:00:00
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更新日期:2019-04-01 00:00:00
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journal_title:British journal of cancer
pub_type: 杂志文章
doi:10.1038/bjc.1983.205
更新日期:1983-09-01 00:00:00
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pub_type: 杂志文章
doi:10.1038/sj.bjc.6603278
更新日期:2006-08-07 00:00:00
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journal_title:British journal of cancer
pub_type: 杂志文章,评审
doi:10.1038/sj.bjc.6604213
更新日期:2008-03-11 00:00:00
abstract::We have purified epithelial cells from human colonic tumours by velocity sedimentation in an isokinetic density gradient of Ficoll in tissue culture medium. In frozen sections of colonic carcinoma, histochemically demonstrable N-acetyl-beta-D-glucosaminidase (HDAG) was observed primarily in epithelial cells. We used t...
journal_title:British journal of cancer
pub_type: 杂志文章
doi:10.1038/bjc.1977.128
更新日期:1977-06-01 00:00:00
abstract::It is known that interferon-gamma (IFN-gamma) is produced by activated T and NK lymphoid cells, mononuclear cells, and macrophage and dendritic cells. Our previous studies have shown that IFN-gamma-like immunoreactivity also appears in human adrenal cortical tumour and phaeochromocytoma. To investigate whether human t...
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pub_type: 杂志文章
doi:10.1038/sj.bjc.6603870
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pub_type: 杂志文章,评审
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pub_type: 杂志文章,评审
doi:10.1038/sj.bjc.6605901
更新日期:2010-10-12 00:00:00
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journal_title:British journal of cancer
pub_type: 杂志文章
doi:10.1038/bjc.1974.126
更新日期:1974-08-01 00:00:00
abstract::Secretary component (SC) is expressed basolaterally as a transmembrane protein (pIg receptor) on secretory epithelial cells. As pIg receptor it plays a central role in humoral immunity by mediating the external translocation of dimeric IgA and pentameric IgM. A few case reports have suggested that reduced or absent SC...
journal_title:British journal of cancer
pub_type: 杂志文章
doi:10.1038/bjc.1996.284
更新日期:1996-06-01 00:00:00
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journal_title:British journal of cancer
pub_type: 杂志文章
doi:10.1038/bjc.1994.317
更新日期:1994-09-01 00:00:00
abstract::Seventy-one patients suffering from acute myeloid leukaemia (AML) who were already in complete remission and had already received one further course of cytotoxic drugs as consolidation therapy were randomised to receive maintenance chemotherapy alone or the same maintenance chemotherapy plus immunotherapy with BCG and...
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pub_type: 临床试验,杂志文章,随机对照试验
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更新日期:1978-01-01 00:00:00
abstract::Photodynamic therapy (PDT) is a form of cancer treatment based on the destruction of cells by the interaction of light, oxygen and a photosensitizer. Aminolaevulinic acid (ALA) is the prodrug of the photosensitizer protoporphyrin IX (PpIX). ALA-induced PDT depends on the rate of cellular synthesis of PpIX, which may v...
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pub_type: 杂志文章
doi:10.1038/bjc.1993.376
更新日期:1993-09-01 00:00:00
abstract::The aim of this study was to assess the cost-effectiveness of combination chemotherapy with paclitaxel/cisplatin, compared with standard etoposide/cisplatin in patients with advanced non-small cell lung cancer (NSCLC). We obtained the primary survival and resource utilization data from a large three-arm randomized tri...
journal_title:British journal of cancer
pub_type: 临床试验,杂志文章,随机对照试验
doi:10.1038/sj.bjc.6690426
更新日期:1999-05-01 00:00:00
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pub_type: 杂志文章
doi:10.1038/bjc.1995.393
更新日期:1995-09-01 00:00:00