Abstract:
:Photodynamic therapy (PDT) is a form of cancer treatment based on the destruction of cells by the interaction of light, oxygen and a photosensitizer. Aminolaevulinic acid (ALA) is the prodrug of the photosensitizer protoporphyrin IX (PpIX). ALA-induced PDT depends on the rate of cellular synthesis of PpIX, which may vary with cell cycle phase. This study has investigated the relationship between cell cycle phase, PpIX generation and phototoxicity in synchronized and unsynchronized bladder cancer cells (HT1197). In unsynchronized cells, relative PpIX fluorescence values (arbitrary units) were significantly different between cell cycle phases after a 1-h ALA incubation (G1 24.8 +/- 0.7; S-phase, 32.7 +/- 0.8, P < 0.05; G2 35.4 +/- 0.8, P < 0.05). In synchronized cells after a 1-h ALA incubation, cells in G1 produced less PpIX than those in S-phase or G2 [6.65 +/- 1.1 ng per 10(5) cells compared with 15.5 +/- 2.1 (P < 0.05), and 8.1 +/- 1.8 ng per 10(5) cells (not significant) respectively] and were significantly less sensitive to ALA-induced PDT (% survival, G1 76.2 +/- 8.3; S-phase 49.7 +/- 4.6, P < 0.05; G2 44.2 +/- 2.4, P < 0.05). This differential response in tumour cells may have implications for clinical PDT, resulting in treatment resistance and possible failure in complete tumour response.
journal_name
Br J Cancerjournal_title
British journal of cancerauthors
Wyld L,Smith O,Lawry J,Reed MW,Brown NJdoi
10.1038/bjc.1998.441subject
Has Abstractpub_date
1998-07-01 00:00:00pages
50-5issue
1eissn
0007-0920issn
1532-1827journal_volume
78pub_type
杂志文章abstract:BACKGROUND:Bortezomib is a proteasome inhibitor with minimal clinical activity as a monotherapy in solid tumours, but its combination with other targeted therapies is being actively investigated as a way to increase its anticarcinogenic properties. Here, we evaluate the therapeutic potential of co-treatment with bortez...
journal_title:British journal of cancer
pub_type: 杂志文章
doi:10.1038/bjc.2011.546
更新日期:2012-01-17 00:00:00
abstract:BACKGROUND:The pathogenesis of lymph node metastases in preinvasive breast cancer – ductal carcinoma in situ (DCIS) – remains controversial. The one-step nucleic acid amplification (OSNA) assay is a novel molecular method that can assess a whole node and detect clinically relevant metastases. In this retrospective coho...
journal_title:British journal of cancer
pub_type: 杂志文章
doi:10.1038/bjc.2012.168
更新日期:2012-05-08 00:00:00
abstract::This study has been designed to verify the specific toxicity of saporin, a type 1 ribosome-inactivating protein (RIP), with the same activity as ricin A chain, targeted by a bispecific monoclonal antibody (bimAb) recognising both the CD25 antigen and the RIP. The CD25 antigen is expressed by lymphoid populations upon ...
journal_title:British journal of cancer
pub_type: 杂志文章
doi:10.1038/bjc.1993.233
更新日期:1993-06-01 00:00:00
abstract::Molecular and immunohistochemical studies of genetic events on chromosome 17p were prospectively compared with conventional clinical and pathological parameters and disease behaviour at a minimum of 72 months follow-up. In a series of 91 patients with primary operable breast cancer, 37 out of 91 (41%) patients had dis...
journal_title:British journal of cancer
pub_type: 杂志文章
doi:10.1038/bjc.1998.129
更新日期:1998-03-01 00:00:00
abstract::Peripheral blood mononuclear cells (PBMCs) were obtained from patients receiving radioactive murine monoclonal antibody (MAb) therapy for malignant epithelial tumours, as well as normal controls, and were tested for the ability of T cells to proliferate in vitro in the presence of the MAb administered for therapy (HMF...
journal_title:British journal of cancer
pub_type: 杂志文章
doi:10.1038/bjc.1991.337
更新日期:1991-09-01 00:00:00
abstract:BACKGROUND:Cisplatin-radiotherapy is a preferred standard for locally advanced, head and neck squamous cell carcinoma (HNSCC). However, the cisplatin-attributable survival benefit is small and toxicity substantial. A biomarker of cisplatin resistance could guide treatment selection and spare morbidity. The ERCC1-XPF nu...
journal_title:British journal of cancer
pub_type: 杂志文章,随机对照试验
doi:10.1038/bjc.2013.576
更新日期:2013-10-15 00:00:00
abstract:BACKGROUND:A frequent mechanism of acquired multidrug resistance in human cancers is overexpression of ATP-binding cassette transporters such as the Multi-Drug Resistance Protein 1 (MDR-1). Nutlin-3, an MDM2-p53 antagonist, has previously been reported to be a competitive MDR-1 inhibitor. METHODS:This study assessed w...
journal_title:British journal of cancer
pub_type: 杂志文章
doi:10.1038/bjc.2014.325
更新日期:2014-08-12 00:00:00
abstract::Sera from patients with nasopharyngeal carcinoma (NPC), a disease associated with Epstein-Barr virus (EBV), were found to be cytotoxic at 15% degrees C in the presence of complement for a panel of human lymphocytes, with a higher frequency than those of matched controls. The cold lymphocytotoxic antibodies (LTA) respo...
journal_title:British journal of cancer
pub_type: 杂志文章
doi:10.1038/bjc.1977.64
更新日期:1977-04-01 00:00:00
abstract:BACKGROUND:In metastatic colorectal cancer (mCRC), KRAS is the only validated biomarker used to select patients for administration of epidermal growth factor receptor (EGFR)-targeted therapies. To identify additional predictive markers, we investigated the importance of HER2, the primary EGFR dimerisation partner, in t...
journal_title:British journal of cancer
pub_type: 杂志文章
doi:10.1038/bjc.2013.4
更新日期:2013-02-19 00:00:00
abstract::The cytochrome P450 CYP1B1 is consistently overexpressed in tumour cells as compared to their normal counterparts, but its precise role in drug resistance is yet to be defined. It has been reported that transfection of CYP1B1 results in increased resistance to docetaxel in V79 cells (McFadyen et al, 2001). In this stu...
journal_title:British journal of cancer
pub_type: 杂志文章
doi:10.1038/sj.bjc.6604195
更新日期:2008-02-12 00:00:00
abstract::We identified 2384 patients in the Danish Cancer Register in whom cancer had been diagnosed in 1960-1996 before they reached the age of 20 and compared them with 53 143 sex- and age-matched controls identified from the Register of Population Statistics. Complete education records and demographic and socioeconomic info...
journal_title:British journal of cancer
pub_type: 杂志文章
doi:10.1038/sj.bjc.6602085
更新日期:2004-08-31 00:00:00
abstract::Growth factor-induced migration is a rate-limiting step in tumour invasiveness. The molecules that regulate this cellular behaviour would represent novel targets for limiting tumour cell progression. Epidermal growth factor (EGF) receptor (EGFR)-mediated motility, present in both autocrine and paracrine modes in prost...
journal_title:British journal of cancer
pub_type: 杂志文章
doi:10.1038/sj.bjc.6603234
更新日期:2006-07-17 00:00:00
abstract:BACKGROUND:Ezrin, which is known as a cytoskeleton linker protein, is closely linked with the metastatic progression of cancer and is frequently abnormally expressed in aggressive cancer types. However, the possible involvement of Ezrin in metastasis and angiogenesis in breast cancer remains unclear. METHODS:Immunohis...
journal_title:British journal of cancer
pub_type: 杂志文章
doi:10.1038/s41416-019-0383-z
更新日期:2019-04-01 00:00:00
abstract::Natural human interferon beta (beta-IFN) was tested during the early phase of in vitro infection with HTLV-I virus of human cord blood mononuclear cells (CBL), to evaluate whether its antiviral and immunomodulating effects might prevent spreading of infection in the host. beta-IFN was found to reduce HTLV-I transmissi...
journal_title:British journal of cancer
pub_type: 杂志文章
doi:10.1038/bjc.1988.111
更新日期:1988-05-01 00:00:00
abstract:BACKGROUND:This study was aimed to develop a new method for personalising chemotherapeutic and granulocyte colony-stimulating factor (G-CSF) combined schedules, and use it for suggesting efficacious chemotherapy with reduced neutropenia. METHODS:Clinical data from 38 docetaxel (Doc)-treated metastatic breast cancer pa...
journal_title:British journal of cancer
pub_type: 杂志文章
doi:10.1038/bjc.2012.316
更新日期:2012-08-21 00:00:00
abstract::An economic sub-study was run alongside a large multi-centre randomised trial (MRC-CR06) comparing three chemotherapy regimens; de Gramont, Lokich and raltitrexed in patients with metastatic colorectal cancer. Patients in six of 45 centres in the main trial were approached to take part in the sub-study. Chemotherapy d...
journal_title:British journal of cancer
pub_type: 临床试验,杂志文章,多中心研究,随机对照试验
doi:10.1038/sj.bjc.6600273
更新日期:2002-06-05 00:00:00
abstract::Vascular endothelial growth factor (VEGF) is recognized to play a predominant role in breast cancer prognosis. The action of VEGF is mediated by two high-affinity receptors with ligand-stimulated tyrosine kinase activity: VEGFR-1/flt-1 and VEGFR-2/flk-1, which are expressed mainly in vascular endothelial cells. To the...
journal_title:British journal of cancer
pub_type: 杂志文章
doi:10.1038/sj.bjc.6692206
更新日期:1999-12-01 00:00:00
abstract:BACKGROUND:We evaluated the efficacy of aprepitant plus granisetron and an increased dose of dexamethasone in selected patients undergoing moderately emetogenic chemotherapy (MEC). METHODS:Nondrinking women <70 years undergoing MEC were randomly assigned to aprepitant (day 1, 125 mg; days 2 and 3, 80 mg) or placebo. D...
journal_title:British journal of cancer
pub_type: 杂志文章,随机对照试验
doi:10.1038/bjc.2013.400
更新日期:2013-08-20 00:00:00
abstract::This paper describes the results of a study set up to investigate factors associated with the high proportions of 'death certificate only' registrations (DCOs) for all cancers registered in south-east England between 1987 and 1989 and to identify those which might be subject to registry intervention. DCOs as a proport...
journal_title:British journal of cancer
pub_type: 杂志文章
doi:10.1038/bjc.1995.125
更新日期:1995-03-01 00:00:00
abstract:BACKGROUND:defective DNA repair has a causal role in hereditary colorectal cancer (CRC). Defects in the base excision repair gene MUTYH are responsible for MUTYH-associated polyposis and CRC predisposition as an autosomal recessive trait. Numerous reports have suggested MUTYH mono-allelic variants to be low penetrance ...
journal_title:British journal of cancer
pub_type: 杂志文章,meta分析
doi:10.1038/sj.bjc.6605966
更新日期:2010-12-07 00:00:00
abstract::Magnetic resonance spectroscopy was used to study intracellular pH and compounds which contain phosphorus in normal human brain and primary brain tumours non-invasively. In normal subjects (n = 7) intracellular pH (pHi) of the brain was 7.03 +/- 0.02 (mean +/- s.e.m.). The pHi did not vary between superficial (2 cm, m...
journal_title:British journal of cancer
pub_type: 杂志文章
doi:10.1038/bjc.1989.300
更新日期:1989-09-01 00:00:00
abstract:BACKGROUND:Though p53 mutations are rare in ES, there is a strong indication that p53 mutant tumours form a particularly bad prognostic group. As such, novel treatment strategies are warranted that would specifically target and eradicate tumour cells containing mutant p53 in this subset of ES patients. METHODS:PRIMA-1...
journal_title:British journal of cancer
pub_type: 杂志文章
doi:10.1038/bjc.2013.635
更新日期:2013-11-12 00:00:00
abstract::We evaluated the therapeutic usefulness of adjuvant chemotherapy in patients with completely resected non-small cell lung cancer (NSCLC). We also examined the relation between DNA ploidy pattern and the response to chemotherapy. A total of 267 patients with NSCLC (pathologically documented stage I, II, or IIIA) underw...
journal_title:British journal of cancer
pub_type: 杂志文章,多中心研究,随机对照试验
doi:10.1038/sj.bjc.6603336
更新日期:2006-10-09 00:00:00
abstract::The mature epidermal growth factor receptor (EGFR) neither associates with nor requires the molecular chaperone heat-shock protein 90 (Hsp90). Mutations in EGFR exons 18, 19, and 21 confer Hsp90 chaperone dependence. In non-small cell lung cancer (NSCLC), these mutations are associated with enhanced sensitivity to EGF...
journal_title:British journal of cancer
pub_type: 杂志文章
doi:10.1038/sj.bjc.6603950
更新日期:2007-09-17 00:00:00
abstract::In small-cell lung cancer cell lines resistance to growth inhibition by transforming growth factor (TGF)-beta 1, was previously shown to correlate with lack of TGF-beta receptor I (RI) and II (RII) proteins. To further investigate the role of these receptors, the expression of mRNA for RI, RII and beta-glycan (RIII) w...
journal_title:British journal of cancer
pub_type: 杂志文章
doi:10.1038/bjc.1996.201
更新日期:1996-05-01 00:00:00
abstract::A retrospective analysis of 321 gastric cancer patients was made to assess the prognostic value of TNM classification, tumour differentiation, Laurén classification, proliferative rate, inflammatory reaction and tumour invasion in vascular or neural structures of the gastric wall. The TNM classification showed the str...
journal_title:British journal of cancer
pub_type: 杂志文章
doi:10.1038/bjc.1996.434
更新日期:1996-09-01 00:00:00
abstract::Clinical data were reviewed in 325 patients with prostatic adenocarcinoma followed up for a mean of 13 years. Paraffin-embedded tumour biopsy specimens from the primary tumours were available for flow cytometry (FCM) in 273 cases. Intra-tumour heterogeneity in DNA index (DI) was found in 4% of the tumours (54 cases we...
journal_title:British journal of cancer
pub_type: 杂志文章
doi:10.1038/bjc.1994.298
更新日期:1994-08-01 00:00:00
abstract::The pathophysiology of Raynaud's phenomenon after Cisplatin-Bleomycin-Vinblastine combination chemotherapy, its relationship to polyneuropathy, and response to transcutaneous nerve stimulation (TNS), was studied in eight patients previously treated for testicular cancer. Peripheral circulation in the index finger was ...
journal_title:British journal of cancer
pub_type: 杂志文章
doi:10.1038/bjc.1991.129
更新日期:1991-04-01 00:00:00
abstract::This study sought to use a microdialysis technique to relate clinical and biochemical responses to the time course of melphalan concentrations in the subcutaneous interstitial space and in tumour tissue (melanoma, malignant fibrous histiocytoma, Merkel cell tumour and osteosarcoma) in patients undergoing regional chem...
journal_title:British journal of cancer
pub_type: 临床试验,杂志文章
doi:10.1054/bjoc.2001.1902
更新日期:2001-07-20 00:00:00
abstract::Mechanisms of resistance to Tomudex include increased thymidylate synthase activity, as well as reduced intracellular drug uptake and polyglutamation. However, little is known about other mechanisms of resistance, such as a possible protection against Tomudex-induced apoptosis mediated by bcl-2. We transfected the MDA...
journal_title:British journal of cancer
pub_type: 杂志文章
doi:10.1038/sj.bjc.6690685
更新日期:1999-09-01 00:00:00