Abstract:
:This study has been designed to verify the specific toxicity of saporin, a type 1 ribosome-inactivating protein (RIP), with the same activity as ricin A chain, targeted by a bispecific monoclonal antibody (bimAb) recognising both the CD25 antigen and the RIP. The CD25 antigen is expressed by lymphoid populations upon activation and by leukaemias and lymphomas with an activated membrane phenotype (Hodgkin's lymphoma, anaplastic large cell lymphoma, adult T cell leukaemia). The bimAb-saporin mixture was tested on CD25+ targets at different bimAb and saporin concentrations. Saporin, in the presence of a bimAb concentration of 10(-9) M, inhibited protein synthesis by CD25+ neoplastic lymphocytes (L540 and MT2 cell lines) with IC50S (concentrations giving 50% of inhibition) ranging from 8 x 10(-12) M to 3 x 10(-11) M. The saporin-bimAb mixture was also effective in blocking the phytohaemagglutinin-driven proliferation of normal lymphocytes, whereas it displayed the same level of toxicity exerted by saporin alone on an irrelevant CD25-negative cell line (EBV-infected B lymphoblastoid cell line). From these results it is possible to envisage a clinical use of this bimAb as a cytotoxic agent for CD25+ leukaemias and lymphomas, as well as an immunosuppressive agent for severe immune disorders such as graft-vs-host disease (GVHD) and transplanted organ rejection.
journal_name
Br J Cancerjournal_title
British journal of cancerauthors
Tazzari PL,Zhang S,Chen Q,Sforzini S,Bolognesi A,Stirpe F,Xie H,Moretta A,Ferrini Sdoi
10.1038/bjc.1993.233subject
Has Abstractpub_date
1993-06-01 00:00:00pages
1248-53issue
6eissn
0007-0920issn
1532-1827journal_volume
67pub_type
杂志文章abstract:BACKGROUND:New therapeutic options for metastatic pancreatic cancer are urgently needed. In pancreatic cancer, overexpression of the epidermal growth factor receptor 2 (HER2) has been reported in up to 45%. This multicentre phase II study investigated the efficacy and toxicity of the HER2 antibody trastuzumab combined ...
journal_title:British journal of cancer
pub_type: 杂志文章,多中心研究
doi:10.1038/bjc.2012.18
更新日期:2012-03-13 00:00:00
abstract::Epidemiological, animal, and cell culture studies have identified boron as a chemopreventative agent in prostate cancer. The present objective was to identify boron-induced changes in the DU-145 human prostate cancer cell line. We show that prolonged exposure to pharmacologically-relevant levels of boric acid, the nat...
journal_title:British journal of cancer
pub_type: 杂志文章
doi:10.1038/sj.bjc.6603009
更新日期:2006-03-27 00:00:00
abstract:BACKGROUND:Focal adhesion kinase (FAK) is overexpressed in a variety of cancers, such as breast, colon, prostate, ovary, and lung cancers. However, the mechanism by which extracellular matrix fibronectin stimulates lung cancer cell migration and invasion through FAK remains to be investigated. METHODS:The signalling p...
journal_title:British journal of cancer
pub_type: 杂志文章
doi:10.1038/sj.bjc.6605154
更新日期:2009-07-21 00:00:00
abstract::A chelating derivative of alpha-melanocyte stimulating hormone (MSH) has been synthesised, in which two molecules of the hormone are cross-linked by diethylenetriamine pentaacetic acid (DTPA). This compound, bisMSH-DTPA, was equipotent with MSH in an in vitro tyrosinase assay with Cloudman S91 melanoma cells. When DBA...
journal_title:British journal of cancer
pub_type: 杂志文章
doi:10.1038/bjc.1990.409
更新日期:1990-12-01 00:00:00
abstract:BACKGROUND:Combined oral contraceptive (COC) use reduces epithelial ovarian cancer (EOC) risk. However, little is known about risk with COC use before the first full-term pregnancy (FFTP). METHODS:This Canadian population-based case-control study (2001-2012) included 854 invasive cases/2139 controls aged ⩾40 years who...
journal_title:British journal of cancer
pub_type: 杂志文章
doi:10.1038/bjc.2016.400
更新日期:2017-01-17 00:00:00
abstract::Soluble and nuclear oestrogen receptor status was determined in both the central and peripheral portions of tumour for 37 cases of adenocarcinoma of the endometrium. Of these, 29 had functional receptor in the peripheral biopsy, but only 19 retained functional receptor in the centre. Six of the 10 patients whose tumou...
journal_title:British journal of cancer
pub_type: 杂志文章
doi:10.1038/bjc.1983.34
更新日期:1983-02-01 00:00:00
abstract:BACKGROUND:Cancer/testis-antigens (CTAs) are specifically expressed in human malignancies and testis tissue, but their molecular functions are poorly understood. CTAs serve as regulators of gene expression, cell cycle and spermatogenesis, as well as targets for immune-based therapies. The CTA PRAME is expressed in vari...
journal_title:British journal of cancer
pub_type: 杂志文章
doi:10.1038/bjc.2016.187
更新日期:2016-08-09 00:00:00
abstract::Eighteen patients with inoperable hepatocellular carcinoma (HCC) were treated with intrahepatic arterial yttrium-90 microspheres. All these patients showed a lung shunting below 15% and a tumour-to-normal ratio higher than 2 as determined by diagnostic technetium-99m macroaggregated albumin (Tc-MAA) gamma scintigraphy...
journal_title:British journal of cancer
pub_type: 临床试验,杂志文章
doi:10.1038/bjc.1994.436
更新日期:1994-11-01 00:00:00
abstract:BACKGROUND:Combined-modality therapy is a promising approach to improve the therapeutic index of radiotherapy. However, these improvements could come at the cost of increased toxicities. Clinical trials evaluating anti-tumour efficacy of bevacizumab combined with radiotherapy have encountered unexpected side effects. T...
journal_title:British journal of cancer
pub_type: 杂志文章
doi:10.1038/bjc.2012.236
更新日期:2012-07-10 00:00:00
abstract::Since 1975, 191 patients with Hodgkin's disease have been treated with a combination of chlorambucil, vinblastine, procarbazine and prednisolone (ChlVPP). Complete remission rates were 73% for previously untreated patients, 91% for patients previously treated with radiotherapy and 55% for patients previously treated w...
journal_title:British journal of cancer
pub_type: 杂志文章
doi:10.1038/bjc.1982.137
更新日期:1982-06-01 00:00:00
abstract:BACKGROUND:The accuracy of prostate-specific antigen (PSA) testing in prostate cancer detection is constrained by low sensitivity and specificity. Dysregulated expression of minichromosome maintenance (Mcm) 2-7 proteins is an early event in epithelial multistep carcinogenesis and thus MCM proteins represent powerful ca...
journal_title:British journal of cancer
pub_type: 杂志文章
doi:10.1038/sj.bjc.6605785
更新日期:2010-08-24 00:00:00
abstract:BACKGROUND:Acute lymphoblastic leukaemia (ALL) is the most common paediatric malignancy. Glucocorticoids form a critical component of chemotherapy regimens and resistance to glucocorticoid therapy is predictive of poor outcome. We have previously shown that glucocorticoid resistance is associated with upregulation of t...
journal_title:British journal of cancer
pub_type: 杂志文章
doi:10.1038/s41416-020-0824-8
更新日期:2020-06-01 00:00:00
abstract:BACKGROUND:Novel prognostic biomarkers and therapeutic strategies are urgently required for malignant melanoma. Ecto-5-prime-nucleotidase (NT5E; CD73) overexpression has been reported in several human cancers. The mechanism(s) underlying deregulated expression and the clinical consequences of changes in expression are ...
journal_title:British journal of cancer
pub_type: 杂志文章
doi:10.1038/bjc.2012.95
更新日期:2012-04-10 00:00:00
abstract::Eleven groups of workers submitted a total of 21 bronchial tumour-associated antigen preparations and 19 antisera for comparative studies. Many of the antisera proved to be polyspecific despite absorption procedures. Most of the antigen preparations contained some material reactive towards a reference antiserum to nor...
journal_title:British journal of cancer
pub_type: 杂志文章
doi:10.1038/bjc.1981.216
更新日期:1981-10-01 00:00:00
abstract::Studies in animal models of mouse mammary carcinoma have shown that ovine submaxillary mucin, which carries multiple sialyl-Tn (STn) epitopes, is effective in stimulating an immune response and inhibiting tumour growth. In similar studies using carbohydrate antigens, pretreatment with low-dose cyclophosphamide has bee...
journal_title:British journal of cancer
pub_type: 临床试验,杂志文章,随机对照试验
doi:10.1038/bjc.1996.532
更新日期:1996-10-01 00:00:00
abstract::We studied 5555 seminoma patients and 3733 patients with nonseminomatous testicular cancers diagnosed in Southeast England between 1960 and 2004. For both groups survival improved over time: 10-year relative survival increased from 78% in 1960-1969 to 99% in 1990-2004 for seminomas, and from 55 to 95% for nonseminomas...
journal_title:British journal of cancer
pub_type: 杂志文章
doi:10.1038/sj.bjc.6603589
更新日期:2007-02-12 00:00:00
abstract::In a prospective cohort study in Denmark of 8736 randomly selected people, no evidence was found among 1011 subjects who developed cancer that self-reported stressful major life events had increased their risk for cancer. ...
journal_title:British journal of cancer
pub_type: 杂志文章
doi:10.1038/sj.bjc.6603471
更新日期:2006-12-04 00:00:00
abstract::Breast cancer is the second most common cancer among women in Madras and southern India after cervix cancer. The Madras Metropolitan Tumour Registry (MMTR), a population-based cancer registry, collects data on the outcome of cancer diagnosis by both active and passive methods. A total of 2080 cases of invasive female ...
journal_title:British journal of cancer
pub_type: 杂志文章
doi:10.1038/bjc.1997.137
更新日期:1997-01-01 00:00:00
abstract::The risk of ovarian epithelial neoplasia following use of hormonal contraceptives (HC) was examined in data from the Norwegian-Swedish Women's Lifestyle and Health cohort including 103551 women aged 30-49 years in 1991-92. Follow-up through 2000 produced 214 incident cases of histologically confirmed epithelial ovaria...
journal_title:British journal of cancer
pub_type: 杂志文章
doi:10.1038/sj.bjc.6601715
更新日期:2004-04-05 00:00:00
abstract::Many theories mention hypersensitive, promiscuous, outlaw or bypass signalling pathways to explain the acquisition of hormone independence in prostate cancer. Hormonal escape of prostate tumours is marked by many biological changes, including mucinous and neuroendocrine differentiation. Since expression of several muc...
journal_title:British journal of cancer
pub_type: 杂志文章
doi:10.1038/sj.bjc.6601570
更新日期:2004-02-09 00:00:00
abstract::Although the presence of axillary node metastases in breast cancer is a key prognostic indicator and may influence treatment decisions, a significant proportion of patients diagnosed as axillary node negative (ANN) using standard histopathological techniques may have occult nodal metastases (OMs). A combination of lim...
journal_title:British journal of cancer
pub_type: 杂志文章
doi:10.1038/bjc.1996.16
更新日期:1996-01-01 00:00:00
abstract::To evaluate whether green tea consumption provides protection against stomach cancer death, relative risks were calculated using Cox proportional hazards regression analysis in the Japan Collaborative Study for Evaluation of Cancer Risk, sponsored by the Ministry of Health and Welfare (JACC Study). The study was based...
journal_title:British journal of cancer
pub_type: 杂志文章
doi:10.1038/sj.bjc.6600487
更新日期:2002-07-29 00:00:00
abstract::The objective of the study was to review the natural history of patients with a malignant pleural effusion but without obvious evidence of a primary, to assess the value of investigations used to look for a primary and to assess the response to palliative chemotherapy. This was done by a retrospective study of patient...
journal_title:British journal of cancer
pub_type: 杂志文章
doi:10.1038/bjc.1996.444
更新日期:1996-09-01 00:00:00
abstract:BACKGROUND:Ovarian cancer is frequently advanced at presentation when treatment is rarely curative. Response to first-line platinum-based chemotherapy significantly influences survival, but clinical response is unpredictable and is frequently limited by the development of drug-resistant disease. METHODS:We used qRT-PC...
journal_title:British journal of cancer
pub_type: 杂志文章
doi:10.1038/bjc.2012.410
更新日期:2012-10-09 00:00:00
abstract::A death certificate-based case-control study was conducted on 207 women aged 25-44 who died of bladder cancer in England and Wales in the period 1971-89 and 411 controls matched on sex, year of death and age at death. An odds ratio of 12.0 (95% CI 1.5-99.7) was found for women with a history of paraplegia. Four of the...
journal_title:British journal of cancer
pub_type: 杂志文章
doi:10.1038/bjc.1994.269
更新日期:1994-07-01 00:00:00
abstract:BACKGROUND:Health-related quality of life (HRQoL) comprises different domains of physical, mental, and social well-being. In this analysis, we focus on sexual quality of life in Hodgkin Lymphoma (HL) patients. METHODS:Four-thousand one-hundred and sixty patients enroled in the HD10-HD12 trials underwent HRQoL assessme...
journal_title:British journal of cancer
pub_type: 杂志文章
doi:10.1038/bjc.2012.550
更新日期:2013-01-15 00:00:00
abstract:BACKGROUND:Bevacizumab has broad anti-tumour activity, but substantial risk of hypertension. No reliable markers are available for predicting bevacizumab-induced hypertension. METHODS:A genome-wide association study (GWAS) was performed in the phase III bevacizumab-based adjuvant breast cancer trial, ECOG-5103, to eva...
journal_title:British journal of cancer
pub_type: 杂志文章,随机对照试验
doi:10.1038/bjc.2014.430
更新日期:2014-09-09 00:00:00
abstract::When assessing the value of a particular treatment, it is important to consider the impact it may have on the quality of life of those being treated. This is particularly so for cancer patients, whose life expectancy may be short. Patients with advanced colorectal cancer who participated in two international comparati...
journal_title:British journal of cancer
pub_type: 临床试验,杂志文章,多中心研究,随机对照试验
doi:10.1038/bjc.1998.420
更新日期:1998-01-01 00:00:00
abstract::Pancreatic cancer is characterised by a hallmark desmoplastic response that includes upregulated expression of the extracellular matrix, and type I collagen in particular. Recent studies indicate that pancreatic cancer cells stimulate type I collagen synthesis in adjacent stellate cells, and that this upregulated type...
journal_title:British journal of cancer
pub_type: 杂志文章
doi:10.1038/sj.bjc.6603088
更新日期:2006-05-08 00:00:00
abstract::This corrects the article DOI: 10.1038/bjc.2017.327. ...
journal_title:British journal of cancer
pub_type: 杂志文章,已发布勘误
doi:10.1038/bjc.2017.438
更新日期:2018-01-01 00:00:00