Abstract：
:The cytochrome P450 CYP1B1 is consistently overexpressed in tumour cells as compared to their normal counterparts, but its precise role in drug resistance is yet to be defined. It has been reported that transfection of CYP1B1 results in increased resistance to docetaxel in V79 cells (McFadyen et al, 2001). In this study, we analysed changes in expression of CYP1B1 mRNA associated with pulse selection of MCF-7 cells with docetaxel. Docetaxel-selected MCF-7 cells (MCF-7 Txt), which showed increased resistance to this drug as compared to parental MCF-7 cells, showed a noteworthy increase in CYP1B1 mRNA expression, paralleled by increased ethoxyresorufin-O-deethylase (EROD) activity levels. This effect was not observed in cisplatin- or adriamycin-selected MCF-7 cells, or in docetaxel-selected colon, lung or pancreatic carcinoma cells. Short-term treatment with docetaxel induced CYP1B1 mRNA expression in MDA 453 and BT-20 breast carcinoma cells, but not in MCF-7 cells. Transfection of MCF-7 Txt cells with CYP1B1 siRNA did not significantly affect docetaxel-induced toxicity, but it decreased cell survival in the absence of drug. Preincubation of docetaxel with recombinant CYP1B1 did not affect drug toxicity in A549 cells. These results suggest that CYP1B1 does not directly inactivate docetaxel, but rather might promote cell survival in MCF-7 Txt cells, providing an explanation for its association with drug resistance.
journal_name
Br J Cancerjournal_title
British journal of cancerauthors
Martinez VG,O'Connor R,Liang Y,Clynes Mdoi
10.1038/sj.bjc.6604195subject
Has Abstractpub_date
2008-02-12 00:00:00pages
564-70issue
3eissn
0007-0920issn
1532-1827pii
6604195journal_volume
98pub_type
杂志文章abstract：:Measurement of DNA polymerase in leukaemic guinea-pig plasms reveals the presence of low levels of sedimentable and non-sedimentable enzymic activities. Since the sedimentable DNA polymerase is ribonuclease sensitive, uses poly(C).oligo(dG) as template, and bands in a sucrose density gradient at 1-17 g/ml it is though...
journal_title：British journal of cancer
pub_type： 杂志文章
doi：10.1038/bjc.1976.67
更新日期：1976-04-01 00:00:00
abstract：BACKGROUND:Gene mutations in the RAS family rule out metastatic colorectal carcinomas (mCRCs) from anti-EGFR therapies. METHODS:We report a retrospective analysis by Sequenom Massarray and fast COLD-PCR followed by Sanger sequencing on 240 mCRCs. RESULTS:By Sequenom, KRAS and NRAS exons 2-3-4 were mutated in 52.9% (1...
journal_title：British journal of cancer
pub_type： 杂志文章
doi：10.1038/bjc.2017.170
更新日期：2017-07-25 00:00:00
abstract：:The incidence of gastro-oesophageal junction (GEJ) adenocarcinoma is increasing in Western countries and prognosis is poor since metastasis is most often present at diagnosis. We examined samples from 87 resected type II GEJ adenocarcinomas, 30 of these with endoscopic diagnostic biopsy material, to evaluate transform...
journal_title：British journal of cancer
pub_type： 杂志文章
doi：10.1054/bjoc.1999.1013
更新日期：2000-02-01 00:00:00
abstract：:Trastuzumab has significantly improved the overall survival of patients with HER2+ metastatic breast cancer (MBC). However, outcomes can vary, with patients progressing within 1 year of treatment or exceptional cases of complete response to trastuzumab for ≥10 years. Identification of the underlying genomic aberration...
journal_title：British journal of cancer
pub_type： 杂志文章
doi：10.1038/s41416-020-0999-z
更新日期：2020-10-01 00:00:00
abstract：:A human prostate tumour cell line, LNCaP C4-2, when injected into athymic male nude mice, produced tumours containing: (1) only human cancer cells similar to those injected; (2) only murine stromal cells containing abnormal chromosome constitutions; or (3) both human prostate cancer cells similar to those injected and...
journal_title：British journal of cancer
pub_type： 杂志文章
doi：10.1038/bjc.1997.524
更新日期：1997-01-01 00:00:00
abstract：:The use of cytotoxic drug vincristine (VCR) is limited by the occurrence of peripheral neuropathy. A neurotrophic ACTH(4-9) analogue, ORG 2766, is being studied for its protective effect. Possible modulatory effects of ORG 2766 on tumour cell growth and interference with the cytotoxic efficacy of VCR were studied in t...
journal_title：British journal of cancer
pub_type： 杂志文章
doi：10.1038/bjc.1994.90
更新日期：1994-03-01 00:00:00
abstract：BACKGROUND:Gender-associated epigenetic alterations are poorly investigated in male and female familial breast cancer (fBC). MicroRNAs may contribute to the different biology in men and women particularly related to RASSF1A pathways. METHODS:Microarray technology was used to evaluate miRNA profile in 24 male and 43 fe...
journal_title：British journal of cancer
pub_type： 杂志文章
doi：10.1038/bjc.2014.535
更新日期：2014-12-09 00:00:00
abstract：:The RNASEL gene on chromosome 1q25 has been identified as a prostate cancer susceptibility gene. We screened for RNASEL germline mutations in familial prostate cancer patients, and performed a case-control study to examine the association of specific variants with prostate cancer risk in the Japanese. Three variants w...
journal_title：British journal of cancer
pub_type： 杂志文章
doi：10.1038/sj.bjc.6601075
更新日期：2003-08-18 00:00:00
abstract：:The hypothesis is advanced that abnormalities of the chromosome group 17,18 play a special role in the genesis and/or evolution of some reticuloendothelial neoplasms. Aberrations of the group 17,18 chromosomes in tumour cells exceed in variety the reported anomaliesof any other chromosome. Both the frequency of these ...
journal_title：British journal of cancer
pub_type： 杂志文章
doi：10.1038/bjc.1970.11
更新日期：1970-03-01 00:00:00
abstract：:To test the anti-tumour activity of rhizoxin in recurrent and/or metastatic squamous cell head and neck cancer, we performed a phase II study. Eligibility required histologically proven squamous cell head and neck cancer. Patients could only have received one prior chemotherapy. Patients were entered if WHO PS was < o...
journal_title：British journal of cancer
pub_type： 临床试验,杂志文章,多中心研究
doi：10.1038/bjc.1996.69
更新日期：1996-02-01 00:00:00
abstract：BACKGROUND:The role of further hormone therapy in castration-resistant prostate cancer (CRPC) remains unclear. We performed a multi-centre randomised phase III study comparing the use of Dexamethasone, Aspirin, and immediate addition of Diethylstilbestrol (DAiS) vs Dexamethasone, Aspirin, and deferred (until disease pr...
journal_title：British journal of cancer
pub_type： 杂志文章,多中心研究,随机对照试验
doi：10.1038/bjc.2011.7
更新日期：2011-02-15 00:00:00
abstract：BACKGROUND:After radical prostatectomy (RP) for prostate cancer (PC), p53 alterations predict biochemical relapse (BCR), however, recent evidence suggests that metastatic relapse (MR) not BCR is a surrogate for PC specific mortality (PCSM). This updated analysis of a previously published study investigated the associat...
journal_title：British journal of cancer
pub_type： 杂志文章
doi：10.1038/s41416-019-0549-8
更新日期：2019-10-01 00:00:00
abstract：:Three hundred and ninety women participated in the quality of life (QL) study of ACCOG1, a high-dose vs conventional adjuvant chemotherapy breast cancer trial, for patients with a high risk of relapse. Patients completed the European Organisation for Research and Treatment of Cancer QLQ-C30, questions on menopausal sy...
journal_title：British journal of cancer
pub_type： 杂志文章
doi：10.1038/sj.bjc.6603454
更新日期：2006-12-18 00:00:00
abstract：:Perforin (PFP) is a cytotoxic protein released from killer cells. PFP immunoreactivity in human peripheral blood lymphocytes (PBL) and tumour infiltrating lymphocytes (TIL) was investigated immunocytochemically with the aid of an anti-PFP monoclonal antibody. PFP was detected in the cytoplasm of 10% of PBL. We perform...
journal_title：British journal of cancer
pub_type： 杂志文章
doi：10.1038/bjc.1991.283
更新日期：1991-08-01 00:00:00
abstract：:Angiotensin II, a main effector peptide in the renin-angiotensin system, acts as a growth-promoting and angiogenic factor via type 1 angiotensin II receptors (AT(1)R). We have recently demonstrated that angiotensin II enhanced tumour cell invasion and vascular endothelial growth factor (VEGF) secretion via AT1R in ova...
journal_title：British journal of cancer
pub_type： 杂志文章
doi：10.1038/sj.bjc.6602961
更新日期：2006-02-27 00:00:00
abstract：:Molecular and immunohistochemical studies of genetic events on chromosome 17p were prospectively compared with conventional clinical and pathological parameters and disease behaviour at a minimum of 72 months follow-up. In a series of 91 patients with primary operable breast cancer, 37 out of 91 (41%) patients had dis...
journal_title：British journal of cancer
pub_type： 杂志文章
doi：10.1038/bjc.1998.129
更新日期：1998-03-01 00:00:00
abstract：:The association between soya foods and breast cancer risk was investigated in a prospective study of 34759 women in Hiroshima and Nagasaki, Japan. Women completed dietary questionnaires in 1969-1970 and/or in 1979-1981 and were followed for incident breast cancer until 1993. The analysis involved 427 cases of primary ...
journal_title：British journal of cancer
pub_type： 杂志文章
doi：10.1038/sj.bjc.6690837
更新日期：1999-12-01 00:00:00
abstract：:Over the past several years, our group has provided considerable evidence that the expression of sigma-2 (sigma2) receptors may serve as a biomarker of tumour cell proliferation. In these in vitro studies, sigma2 receptors were expressed 8-10 times more in proliferative (P) tumour cells than in quiescent (Q) tumour ce...
journal_title：British journal of cancer
pub_type： 杂志文章
doi：10.1054/bjoc.1999.1067
更新日期：2000-03-01 00:00:00
abstract：:We examined the levels of expression of the multidrug resistance-associated protein (MRP) gene quantified by Northern blot analysis in comparison with those of the MDR1 gene determined by reverse transcription-polymerase chain reaction (RT-PCR) in 104 non-small-cell lung cancer (NSCLC) specimens [59 adenocarcinoma (Ad...
journal_title：British journal of cancer
pub_type： 杂志文章
doi：10.1038/bjc.1995.372
更新日期：1995-09-01 00:00:00
abstract：:Previous studies have demonstrated that BR-931, a hepatic peroxisome proliferator, can induce liver tumours in mice and rats. Since alterations in gene expression may play a critical role in multistage hepatocarcinogenesis, the present studies examined the expression of the c-myc, c-H-ras, epidermal growth factor (EGF...
journal_title：British journal of cancer
pub_type： 杂志文章
doi：10.1038/bjc.1991.406
更新日期：1991-11-01 00:00:00
abstract：:In this study, the role of parity in conferring protection of the mammary gland against chemical carcinogenesis induced by 7,12-dimethylbenz(a)anthracene (DMBA) was investigated. Experiments were also carried out to determine if an 'interrupted' pregnancy was capable of reducing the incidence of mammary tumour inducti...
journal_title：British journal of cancer
pub_type： 杂志文章
doi：10.1038/bjc.1988.88
更新日期：1988-04-01 00:00:00
abstract：:The normal-lymphocyte-transfer test has been advocated as a method of assessing the immuno-competence of lymphocytes from patients with breast and large-bowel cancer. Evidence is presented in this paper that the methodology is subject to many uncontrollable errors, that the reaction is the result of multiple factors, ...
journal_title：British journal of cancer
pub_type： 杂志文章
doi：10.1038/bjc.1978.219
更新日期：1978-09-01 00:00:00
abstract：:Cell fusion has been used to analyse the genetic determinants of metastasis at the cellular level. Highly metastatic mouse melanoma cells were fused with diploid mouse lymphocytes and a range of hybrid clones isolated and tested for tumorigenicity and metastatic potential by s.c. injection into newborn, histocompatibl...
journal_title：British journal of cancer
pub_type： 杂志文章
doi：10.1038/bjc.1983.60
更新日期：1983-03-01 00:00:00
abstract：:The relationship between hepatic arterial albumin microsphere distribution and hepatic arterial blood flow and the effects of regional angiotensin II were studied in a rat liver metastases model. Hooded-Lister rats were inoculated subcapsularly with 2 x 10(6) HSN sarcoma cells. At 20 days, hepatic arterial blood flow ...
journal_title：British journal of cancer
pub_type： 杂志文章
doi：10.1038/bjc.1992.258
更新日期：1992-08-01 00:00:00
abstract：:The topical carcinogenicity to mouse skin of smoke condensates obtained from a tobacco substitute (NSM), alone or in combination with tobacco, has been compared with condensate from tobacco and with acetone, the solvent used. Sixteen different types of cigarette were used to make the condensates, and the age-standardi...
journal_title：British journal of cancer
pub_type： 杂志文章
doi：10.1038/bjc.1977.47
更新日期：1977-03-01 00:00:00
abstract：:To our knowledge, very few data about the role of Topoisomerase IIalpha (TOPO-IIalpha), an enzyme involved in critical steps of tumour cell proliferation and chemoresistance are currently available in ovarian cancer patients. The aim of this study was to investigate the prognostic value of TOPO-IIalpha expression in a...
journal_title：British journal of cancer
pub_type： 杂志文章
doi：10.1038/sj.bjc.6604410
更新日期：2008-06-17 00:00:00
abstract：:Follicular-phase (Day 11) plasma prolactin, and plasma and urinary oestrogen levels of 70 nulliparous nuns were compared with those of 80 of their sisters, of whom 62 were parous. The nuns and their nulliparous sisters did not differ significantly in their prolactin and oestrogen levels. No differences in plasma oestr...
journal_title：British journal of cancer
pub_type： 杂志文章
doi：10.1038/bjc.1981.121
更新日期：1981-06-01 00:00:00
abstract：:BW12C (5-[2-formyl-3-hydroxypenoxyl] pentanoic acid) stabilizes oxyhaemoglobin, causing a reversible left-shift of the oxygen saturation curve (OSC) and tissue hypoxia. The activity of mitomycin C (MMC) is enhanced by hypoxia. In this phase II study, 17 patients with metastatic colorectal cancer resistant to 5-fluorou...
journal_title：British journal of cancer
pub_type： 临床试验,杂志文章,随机对照试验
doi：10.1054/bjoc.2000.1138
更新日期：2000-06-01 00:00:00
abstract：BACKGROUND:Metastatic clear cell renal cell carcinoma (ccRCC) patients have <9% 5-year survival rate, do not respond well to targeted therapy and eventually develop resistance. A better understanding of molecular pathways of RCC metastasis is the basis for the discovery of novel prognostic markers and targeted therapie...
journal_title：British journal of cancer
pub_type： 杂志文章
doi：10.1038/bjc.2013.828
更新日期：2014-03-04 00:00:00
abstract：BACKGROUND:This Phase Ib dose-escalating study investigated safety, maximum tolerated dose (MTD), dose-limiting toxicity (DLT), pharmacokinetics (PK) and clinical antitumour activity of tosedostat (CHR-2797), an orally bioavailable aminopeptidase inhibitor, in combination with paclitaxel. METHODS:A total of 22 patient...
journal_title：British journal of cancer
pub_type： 杂志文章
doi：10.1038/sj.bjc.6605917
更新日期：2010-10-26 00:00:00
