Abstract：
BACKGROUND:Metastatic clear cell renal cell carcinoma (ccRCC) patients have <9% 5-year survival rate, do not respond well to targeted therapy and eventually develop resistance. A better understanding of molecular pathways of RCC metastasis is the basis for the discovery of novel prognostic markers and targeted therapies. METHODS:We investigated the biological impact of galectin-1 (Gal-1) in RCC cell lines by migration and invasion assays. Effect of Gal-1 expression on the mitogen-activated protein kinase pathway was assessed by proteome array. RESULTS:Increased expression of Gal-1 increased cell migration while knocking down Gal-1 expression by siRNA resulted in reduced cellular migration (P<0.001) and invasion (P<0.05). Gal-1 overexpression increased phosphorylation of Akt, mTOR and p70 kinase. Upon hypoxia and increased HIF-1α, Gal-1 increased in a dose-dependent manner. We also found miR-22 overexpression resulted in decreased Gal-1 and HIF-1α. Immunohistochemistry analysis showed that high Gal-1 protein expression was associated with larger size tumor (P=0.034), grades III/IV tumors (P<0.001) and shorter disease-free survival (P=0.0013). Using the Cancer Genome Atlas data set, we found that high Gal-1 mRNA expression was associated with shorter overall survival (41 vs 78 months; P<0.01). CONCLUSIONS:Our data suggest Gal-1 mediates migration and invasion through the HIF-1α-mTOR signaling axis and is a potential prognostic marker and therapeutic target.
journal_name
Br J Cancerjournal_title
British journal of cancerauthors
White NM,Masui O,Newsted D,Scorilas A,Romaschin AD,Bjarnason GA,Siu KW,Yousef GMdoi
10.1038/bjc.2013.828subject
Has Abstractpub_date
2014-03-04 00:00:00pages
1250-9issue
5eissn
0007-0920issn
1532-1827pii
bjc2013828journal_volume
110pub_type
杂志文章abstract：:The identification of peptide vaccine candidates to date has been focused on human leukocyte antigen (HLA)-A2 and -A24 alleles. In this study, we attempted to identify cytotoxic T lymphocyte (CTL)-directed Lck-derived peptides applicable to HLA-A11(+), -A31(+), or -A33(+) cancer patients, because these HLA-A alleles s...
journal_title：British journal of cancer
pub_type： 杂志文章
doi：10.1038/sj.bjc.6604071
更新日期：2007-12-17 00:00:00
abstract：:High-mol.-wt levan injected locally inhibits the growth of Lewis lung carcinoma in C57BL mice. The inhibition is dependent on the number of tumour cells injected and on the dose of levan. The inhibition decreases tumour incidence and size as well as prolonging survival. The polysaccharide is most effective when inject...
journal_title：British journal of cancer
pub_type： 杂志文章
doi：10.1038/bjc.1979.225
更新日期：1979-10-01 00:00:00
abstract：:Adriamycin and cyclophosphamide are active agents in human and experimental tumours. Using the L1210 murine leukaemia, their effectiveness alone and in combination was studied. The combination is highly synergistic in this tumour, resulting in a greater than 50% survival rate when the agents used alone at optimal dose...
journal_title：British journal of cancer
pub_type： 杂志文章
doi：10.1038/bjc.1975.150
更新日期：1975-08-01 00:00:00
abstract：BACKGROUND:The National Comprehensive Cancer Network (NCCN) guidelines provide recommendations for staging of prostate cancer patients in the objective regarding presence of locoregional lymph node metastases (LNM) and bone metastases. We tested the performance characteristics of these recommendations in a community se...
journal_title：British journal of cancer
pub_type： 杂志文章
doi：10.1038/s41416-018-0323-3
更新日期：2018-12-01 00:00:00
abstract：:The influence of anaesthetics on the in vivo response of B16 melanoma to melphalan was studied using an in vitro cell-survival assay. Three anaesthetics were used, Saffan (Althesin) Sagatal (Nembutal) and Hypnorm. When Saffan was administered to tumour-bearing animals before melphalan there was a significant increase ...
journal_title：British journal of cancer
pub_type： 杂志文章
doi：10.1038/bjc.1978.279
更新日期：1978-12-01 00:00:00
abstract：BACKGROUND:The role of processed meat in the aetiology of several cancers was explored in detail. METHODS:In the time period 1996-2004, a multisite case-control study was conducted in Montevideo, Uruguay. The study included 6 060 participants (3 528 cases and 2 532 controls) corresponding to cancers of the oral cavity...
journal_title：British journal of cancer
pub_type： 杂志文章,多中心研究
doi：10.1038/bjc.2012.433
更新日期：2012-10-23 00:00:00
abstract：:Loss of heterozygosity (LOH) on chromosome 17q21 has been detected in 30% of primary human breast tumours. The smallest common region deleted occurred in an interval between the D17S746 and D17S846 polymorphic sequences tagged sites that are located on two recombinant P1-bacteriophage clones of chromosome 17q21: 122F4...
journal_title：British journal of cancer
pub_type： 杂志文章
doi：10.1038/sj.bjc.6601848
更新日期：2004-06-14 00:00:00
abstract：:Antibody drug conjugates (ADCs) employ the exquisite specificity of tumour-specific monoclonal antibodies (mAb) for the targeted delivery of highly potent cytotoxic drugs to the tumour site. The chemistry of the linker, which connects the drug to the mAb, determines how and when the drug is released from the mAb. This...
journal_title：British journal of cancer
pub_type： 杂志文章,评审
doi：10.1038/bjc.2017.367
更新日期：2017-12-05 00:00:00
abstract：BACKGROUND:Systemic inflammation measured by the neutrophil-to-lymphocyte ratio (NLR), leucocyte-to-lymphocyte ratio (LLR), platelet-to-lymphocyte ratio (PLR), monocyte-to-lymphocyte ratio (MLR) and CRP/albumin ratio (CRP/Alb) was shown to impact the survival prognosis in patients with extracranial solid cancer. METHO...
journal_title：British journal of cancer
pub_type： 杂志文章
doi：10.1038/s41416-020-01254-0
更新日期：2021-01-21 00:00:00
abstract：:Leukaemia L 1210 cells preincubated in vitro with neuraminidase preparations derived either from Vibrio cholerae (VCN) or Clostridium perfringens (CPN) lost their i.p. transplantability for normal DBA/2 mice. This loss of transplantability could not be observed when the enzyme treated cells were implanted in mice whos...
journal_title：British journal of cancer
pub_type： 杂志文章
doi：10.1038/bjc.1973.14
更新日期：1973-02-01 00:00:00
abstract：:We determined the prevalence of type-specific hrHPV infections in the Netherlands on cervical scrapes of 45 362 women aged 18-65 years. The overall hrHPV prevalence peaked at the age of 22 with peak prevalence of 24%. Each of the 14 hrHPV types decreased significantly with age (P-values between 0.0009 and 0.03). The p...
journal_title：British journal of cancer
pub_type： 杂志文章
doi：10.1038/sj.bjc.6604162
更新日期：2008-02-12 00:00:00
abstract：:With the recent approval of the combinations of axitinib with the immune checkpoint inhibitor (ICI) pembrolizumab or avelumab for first-line treatment of advanced renal cell carcinoma, guidance on how to distinguish between immune-related adverse events (AEs) caused by ICI versus axitinib-related AEs is necessary to o...
journal_title：British journal of cancer
pub_type： 杂志文章,评审
doi：10.1038/s41416-020-0949-9
更新日期：2020-09-01 00:00:00
abstract：:The serum CA125 level was determined by a one-step immunoradiometric assay method in patients with lung cancer. Increased serum CA125 levels were observed in 37.8% of patients with squamous cell cancer, in 30.0% of those with adenocarcinoma and in 60.0% of those with small call cancer. Most patients with increased ser...
journal_title：British journal of cancer
pub_type： 杂志文章
doi：10.1038/bjc.1990.355
更新日期：1990-10-01 00:00:00
abstract：:The effects of polymorphisms in genes coding for key folate metabolism enzymes such as thymidylate synthetase (TS) on colorectal neoplasia risk are likely to be influenced by gene-gene and gene-nutrient interactions. We investigated the combined effects of three polymorphisms in the TS gene region, TSER, TS 3R G>C, an...
journal_title：British journal of cancer
pub_type： 杂志文章
doi：10.1038/sj.bjc.6604056
更新日期：2007-11-19 00:00:00
abstract：:We screened 145 HIV-infected non-pregnant women at a tertiary care centre in Lusaka, Zambia. Liquid-based cytology and human papillomavirus (HPV) genotyping with PGMY09/11 biotinylated primers (Roche Linear Array HPV genotyping test) maximised sensitivity of cytology and HPV assessments. Among high-risk (HR) types, HP...
journal_title：British journal of cancer
pub_type： 杂志文章
doi：10.1038/sj.bjc.6603737
更新日期：2007-05-07 00:00:00
abstract：:Nineteen benign human breast tumours, from 19 premenopausal patients, were processed into slices and each tumour was individually cultured for 2 days in Medium 199. The effects of bovine insulin (5.0 microgram/ml) human placental lactogen (10.0 microgram/ml) and human growth hormone (10.0 microgram/ml) on 3H-thymidine...
journal_title：British journal of cancer
pub_type： 杂志文章
doi：10.1038/bjc.1978.196
更新日期：1978-08-01 00:00:00
abstract：:Chordomas are radio- and chemo-resistant tumours and metastasise in as many as 40% of patients. The aim of this study was to identify potential molecular targets for the treatment of chordoma. In view of the reported association of chordoma and tuberous sclerosis complex syndrome, and the available therapeutic agents ...
journal_title：British journal of cancer
pub_type： 杂志文章
doi：10.1038/sj.bjc.6605019
更新日期：2009-05-05 00:00:00
abstract：:In addition to the direct targeting effects on HER2-positive cells, trastuzumab may have a therapeutic role modulating the activity of the cellular immune system in patients with breast cancer. To investigate this further, the balance of T-regulatory (T(reg)), Th17, natural killer (NK) and NK T (NKT) cells before, dur...
journal_title：British journal of cancer
pub_type： 杂志文章
doi：10.1038/sj.bjc.6604963
更新日期：2009-04-07 00:00:00
abstract：:Previously, we reported that Bcl-2 was frequently expressed in renal cell carcinoma (RCC) specimens, but p53 mutation was a rare event. However, it was unclear whether Bcl-2 positivity was associated with the clinicopathological characteristics and prognosis in RCC. Therefore, we investigated the expression of Bcl-2 p...
journal_title：British journal of cancer
pub_type： 杂志文章
doi：10.1038/sj.bjc.6601454
更新日期：2004-01-12 00:00:00
abstract：:In some clinical situations the endogenous production of glutamine may be insufficient to maintain optimal tissue structure and function such that glutamine becomes a conditionally essential amino acid. Studies in laboratory animals have demonstrated that glutamine supplementation can reduce the incidence and severity...
journal_title：British journal of cancer
pub_type： 临床试验,杂志文章,随机对照试验
doi：10.1038/bjc.1994.385
更新日期：1994-10-01 00:00:00
abstract：:Photodynamic activation of macrophage-like cells contributes to an effective outcome of photodynamic therapy (PDT) treatment. The possibility for an enhancement of macrophage activity by photosensitization with meta-tetra(hydroxyphenyl)chlorin (mTHPC) (1 microg ml(-1)) was studied in U937, monocyte cell line different...
journal_title：British journal of cancer
pub_type： 杂志文章
doi：10.1038/sj.bjc.6690648
更新日期：1999-09-01 00:00:00
abstract：:A number of rare human inherited syndromes are associated with apparent defects in DNA repair and a greatly increased frequency of cancer. Cell lines derived from such individuals phenotypically resemble certain bacterial mutant strains that have increased sensitivity to radiation or chemical agents and well character...
journal_title：British journal of cancer
pub_type： 杂志文章,评审
doi：10.1038/bjc.1987.163
更新日期：1987-08-01 00:00:00
abstract：BACKGROUND:The precise timing of the angiogenic switch and the role of angiogenesis in the development of breast malignancy is currently unknown. METHODS:Therefore, the expression of CD31 (pan endothelial cells (ECs)), endoglin (actively proliferating ECs), hypoxia-inducible factor-1 (HIF-1alpha), vascular endothelial...
journal_title：British journal of cancer
pub_type： 杂志文章
doi：10.1038/sj.bjc.6605196
更新日期：2009-08-18 00:00:00
abstract：:In a longitudinal study of the evolution of serum beta 2-microglobulin (beta 2-m) levels in 37 patients with myelomatosis, those patients with a level of < 4 mg/l at first presentation had a median survival of 46 months, whereas those with an initial level of > mg/l had a median survival of 15 months. THe beta 2-m app...
journal_title：British journal of cancer
pub_type： 杂志文章
doi：10.1038/bjc.1980.273
更新日期：1980-10-01 00:00:00
abstract：:Previous studies have reported the clinical usefulness of reverse transcription-polymerase chain reaction (RT-PCR) detection of thyroglobulin (TG) mRNA in the peripheral blood of patients with differentiated thyroid carcinoma. To evaluate this usefulness, we measured TG mRNA in the peripheral blood of patients diagnos...
journal_title：British journal of cancer
pub_type： 杂志文章
doi：10.1054/bjoc.2001.1904
更新日期：2001-07-06 00:00:00
abstract：BACKGROUND:Mechanisms that increase resistance to apoptosis help promote cellular transformation. Cancer cells have deregulated apoptotic pathways, where increased expression and stability of anti-apoptotic proteins Mcl-1 and Bcl-2 increases resistance to apoptosis. Pathways that increase the stability of proteins in c...
journal_title：British journal of cancer
pub_type： 杂志文章
doi：10.1038/bjc.2011.242
更新日期：2011-07-26 00:00:00
abstract：BACKGROUND:Most studies use hospital data to calculate postoperative complication rates (PCRs). We report on improving PCR estimates through use of patient-reporting. METHODS:A prospective cohort study of major surgery performed at 10 UK gynaecological cancer centres was undertaken. Hospitals entered the data contempo...
journal_title：British journal of cancer
pub_type： 杂志文章,多中心研究
doi：10.1038/bjc.2013.366
更新日期：2013-08-06 00:00:00
abstract：:Cellular adhesion molecules have been implicated in tumour progression and metastasis. This study examines for the first time the serum concentrations of circulating VCAM-1 and E-selectin in a consecutive series of 110 cancer patients seen in a general medical oncology clinic, and confirms and extends previous studies...
journal_title：British journal of cancer
pub_type： 杂志文章
doi：10.1038/bjc.1993.298
更新日期：1993-07-01 00:00:00
abstract：:Chlamydia pneumoniae, Chlamydia trachomatis and Chlamydia psittaci were detected at low frequencies (<20%) among 69 pulmonary mucosa-associated lymphoid tissue (MALT) lymphomas, 30 other lymphoproliferative disorders (LPD) and 44 non-LPD. The incidence of individual Chlamydiae was generally higher in MALT lymphoma tha...
journal_title：British journal of cancer
pub_type： 杂志文章
doi：10.1038/sj.bjc.6603981
更新日期：2007-10-08 00:00:00
abstract：:The formation of cyclic AMP was studied in normal liver, subcutaneous hepatomas derived from MH1C1 cells, and premalignant liver and primary hepatomas induced by the carcinogens 2-acetylaminofluorene (AAF) and 4-dimethylamino-azobenzene (DAB). While only very slight effects of prostaglandins (PG) were seen in slices o...
journal_title：British journal of cancer
pub_type： 杂志文章
doi：10.1038/bjc.1978.281
更新日期：1978-12-01 00:00:00
