Abstract：
:We have purified epithelial cells from human colonic tumours by velocity sedimentation in an isokinetic density gradient of Ficoll in tissue culture medium. In frozen sections of colonic carcinoma, histochemically demonstrable N-acetyl-beta-D-glucosaminidase (HDAG) was observed primarily in epithelial cells. We used this enzyme as a histochemical marker of epithelial cells. Initial suspensions of cells from colonic tumours suspended with 0-25% trypsin contained an average of 24% of the nucleated cells with HDAG. In the purest fraction obtained from gradient centrifugations, an average of 74% of the nucleated cells contained HDAG. After centrifugation, the quarter of the density gradient which contained the most rapidly sedimenting cells was purified 2-4-fold over that in the initial suspension. Cells in this zone of the gradient also gave rise to colonies in soft agar. Cells from initial suspension resulted in 15-25% as many colonies of 7 or more cells in cultures inoculated with the same number of nucleated cells. For the most part, cells obtained from the other zones of the gradient did not give rise to colonies in soft agar.
journal_name
Br J Cancerjournal_title
British journal of cancerauthors
Brattain MG,Kimball PM,Pretlow TG 2nd,Pitts AMdoi
10.1038/bjc.1977.128subject
Has Abstractpub_date
1977-06-01 00:00:00pages
850-7issue
6eissn
0007-0920issn
1532-1827journal_volume
35pub_type
杂志文章abstract：:Trastuzumab is the only HER2/neu-directed therapy to have received Food and Drug Administration approval for the treatment of patients with metastatic breast cancer. The efficacy of trastuzumab depends on the HER2/neu status of the tumour and the patient's prior treatment, but even when patients are selected on the ba...
journal_title：British journal of cancer
pub_type： 杂志文章
doi：10.1038/sj.bjc.6602926
更新日期：2006-01-30 00:00:00
abstract：BACKGROUND:Period analysis is increasingly used to compute long-term cancer survival, as it provides better prediction of survival of newly diagnosed patients than traditional cohort analysis. However, the patient population to which period survival estimates best pertain to and which should be described in a study is ...
journal_title：British journal of cancer
pub_type： 杂志文章
doi：10.1038/bjc.2013.14
更新日期：2013-02-19 00:00:00
abstract：:Tamoxifen is the current therapy of choice for patients with oestrogen receptor positive breast cancer, and it is currently under evaluation as a prophylactic for women at high risk of developing the disease. However, tamoxifen is also known to induce proliferative changes in the endometrium increasing the risk of dev...
journal_title：British journal of cancer
pub_type： 杂志文章
doi：10.1038/sj.bjc.6600157
更新日期：2002-03-04 00:00:00
abstract：BACKGROUND:Selecting patients with 'sufficient life expectancy' for Phase I oncology trials remains challenging. The Royal Marsden Hospital Score (RMS) previously identified high-risk patients as those with ≥ 2 of the following: albumin <35 g l(-1); LDH > upper limit of normal; >2 metastatic sites. This study developed...
journal_title：British journal of cancer
pub_type： 杂志文章
doi：10.1038/bjc.2012.371
更新日期：2012-09-25 00:00:00
abstract：:Imatinib mesilate (Glivec), Novartis Pharmaceuticals) is a novel therapy for the treatment of chronic myeloid leukaemia (CML). We evaluated the cost-effectiveness of imatinib (600 mg daily) when used for the treatment of patients in advanced stages of CML (accelerated phase and blast crisis) against conventional thera...
journal_title：British journal of cancer
pub_type： 杂志文章
doi：10.1038/sj.bjc.6601151
更新日期：2003-08-18 00:00:00
abstract：BACKGROUND:Administration of interleukin-2 (IL-2) has shown some effects on malignant pleural mesothelioma (MPM) tumour regression. The purpose of this study was to investigate the ability of IL-2 to modify immunological effector cells and angiogenesis in MPM patients and their prognostic value. METHODS:Tumour-infiltr...
journal_title：British journal of cancer
pub_type： 杂志文章
doi：10.1038/sj.bjc.6605438
更新日期：2009-12-01 00:00:00
abstract：:The prognostic value of several clinical and histopathological characteristics has been evaluated in patients with Dukes' B and C carcinoma of the rectum and the rectosigmoid. Data on 260 Dukes' B and 208 Dukes' C tumours entered into a prospective, randomized clinical trial of post-operative radiotherapy (50 Gy given...
journal_title：British journal of cancer
pub_type： 杂志文章
doi：10.1038/bjc.1988.192
更新日期：1988-08-01 00:00:00
abstract：:A case-control study of lung cancer involving interviews with 965 female patients and 959 controls in Shenyang and Harbin, two industrial cities which have among the highest rates of lung cancer in China, revealed that cigarette smoking is the main causal factor and accounted for about 35% of the tumours among women. ...
journal_title：British journal of cancer
pub_type： 杂志文章
doi：10.1038/bjc.1990.421
更新日期：1990-12-01 00:00:00
abstract：:In mice given a single dose of diethylnitrosamine, a hepatonecrotic dose of carbon tetrachloride, 5 weeks after dosing with DEN and repeated 6 times at 4-weekly intervals, augmented the tumour yield in the livers. A single hepatonecrotic dose of CCl4 24 h before a single dose of DEN also increased the number of tumour...
journal_title：British journal of cancer
pub_type： 杂志文章
doi：10.1038/bjc.1978.89
更新日期：1978-04-01 00:00:00
abstract：:Patients who have no residual invasive cancer following neoadjuvant chemotherapy for breast carcinoma have a better overall survival than those with residual disease. Many classification systems assessing pathological response to neoadjuvant chemotherapy include residual ductal carcinoma in situ (DCIS) only in the def...
journal_title：British journal of cancer
pub_type： 杂志文章
doi：10.1038/sj.bjc.6602950
更新日期：2006-02-13 00:00:00
abstract：:The biguanide drug metformin profoundly affects cell metabolism, causing an impairment of the cell energy balance and triggering a plethora of pleiotropic effects that vary depending on the cellular or environmental context. Interestingly, a decade ago, it was observed that metformin-treated diabetic patients have a s...
journal_title：British journal of cancer
pub_type： 杂志文章,评审
doi：10.1038/bjc.2016.385
更新日期：2016-12-06 00:00:00
abstract：:A bispecific monoclonal antibody recognising both carcinoembryonic antigen (CEA) and ricin toxin A chain (RTA) was tested for its ability to target recombinant RTA (r-RTA) to CEA-expressing tumour cells, alone and in combination with ricin B chain (RTB). The antibody, 636 (Robins et al., 1990), induced significant RTA...
journal_title：British journal of cancer
pub_type： 杂志文章
doi：10.1038/bjc.1991.153
更新日期：1991-05-01 00:00:00
abstract：BACKGROUND:Diet modulates inflammation and inflammatory markers have been associated with cancer outcomes. In the Women's Health Initiative, we investigated associations between a dietary inflammatory index (DII) and invasive breast cancer incidence and death. METHODS:The DII was calculated from a baseline food freque...
journal_title：British journal of cancer
pub_type： 杂志文章
doi：10.1038/bjc.2016.98
更新日期：2016-05-24 00:00:00
abstract：:Flavone acetic acid (FAA) was administered at a dose of 4.8 g m-2 over 1 h to patients with advanced malignant disease in combination with Interleukin II. A new high performance liquid chromatography method is described to determine both the parent compound and eight drug-related products, and the conditions required ...
journal_title：British journal of cancer
pub_type： 杂志文章
doi：10.1038/bjc.1993.250
更新日期：1993-06-01 00:00:00
abstract：:Ninety-nine patients with advanced breast cancer were randomized to receive either cyclophosphamide continuously or a combination of cyclophosphamide, methotrexate, 5-fluorouracil and vinblastine given intermittently. The number and duration of objective responses were greater in patients receiving the combination but...
journal_title：British journal of cancer
pub_type： 临床试验,杂志文章,随机对照试验
doi：10.1038/bjc.1975.284
更新日期：1975-12-01 00:00:00
abstract：BACKGROUND:Multiple early gastric cancers (EGCs) may develop in 6-14% of patients even after achieving curative endoscopic submucosal dissection (ESD); however, a useful biomarker for predicting recurrence is not available. The present study investigated whether the expression of CD44 variant 9 (CD44v9), a functional c...
journal_title：British journal of cancer
pub_type： 杂志文章
doi：10.1038/bjc.2013.314
更新日期：2013-07-23 00:00:00
abstract：:Chinese hamster V79-171B spheroids implanted in the peritoneal cavity of C3H mice were characterized as a model for evaluating the toxicity of drugs requiring metabolic activation in vivo. After 24 hours in the peritoneal cavity, spheroid cellularity and plating efficiency were not significantly decreased, and host ce...
journal_title：British journal of cancer
pub_type： 杂志文章
doi：10.1038/bjc.1987.196
更新日期：1987-09-01 00:00:00
abstract：BACKGROUND:Pancreatic stellate cells (PSCs, which produce the stroma of pancreatic cancer (PC)) interact with cancer cells to facilitate PC growth. A candidate growth factor pathway that may mediate this interaction is the HGF-c-MET pathway. METHODS:Effects of HGF inhibition (using a neutralising antibody AMG102) alon...
journal_title：British journal of cancer
pub_type： 杂志文章
doi：10.1038/bjc.2015.478
更新日期：2016-02-02 00:00:00
abstract：BACKGROUND:The host immune reaction is represented by immune/inflammatory cell infiltrates. Here we systematically analysed tumour-infiltrating immune/inflammatory cells in pancreatic ductal carcinoma (PDC) and evaluated their clinicopathological impact. METHODS:Using immunohistochemistry, we examined tumour-infiltrat...
journal_title：British journal of cancer
pub_type： 杂志文章
doi：10.1038/bjc.2013.32
更新日期：2013-03-05 00:00:00
abstract：:The aetiologic fractions due to smoking and chewing tobacco have been quantified for the first time, for cancers of the oral cavity, oropharynx, hypopharynx, larynx and oesophagus. The overall aetiologic fractions due to smoking and/or chewing tobacco have been found to be 70% for cancer of the oral cavity, 84% for th...
journal_title：British journal of cancer
pub_type： 杂志文章
doi：10.1038/bjc.1977.31
更新日期：1977-02-01 00:00:00
abstract：BACKGROUND:We used bleomycin, etoposide, cisplatin (BEP), the most effective regimen in the treatment of germ cell tumours (GCTs) and increased dose-density by using pegfilgrastim to shorten cycle length. Our aim was to assess safety and tolerability. METHODS:Sixteen male patients with intermediate or poor prognosis m...
journal_title：British journal of cancer
pub_type： 杂志文章
doi：10.1038/bjc.2011.309
更新日期：2011-09-06 00:00:00
abstract：:Telomerase is a specialized ribonucleoprotein polymerase that directs the synthesis of telomerase repeats at chromosome ends. Accumulating evidence has indicated that telomerase is stringently repressed in normal human somatic tissues but reactivated in cancers and immortal cells, suggesting that activation of telomer...
journal_title：British journal of cancer
pub_type： 杂志文章
doi：10.1038/bjc.1998.363
更新日期：1998-06-01 00:00:00
abstract：:Solid tumour cells were shown to express VLA-beta and Thy-1 antigens. For identification of these molecules two monoclonal antibodies, K-20 and ICO-10, characterised in detail previously, were used. Four groups of solid tumours have been identified according to their immunophenotype: VLA-beta+ and Thy-1-; VLA-beta+ an...
journal_title：British journal of cancer
pub_type： 杂志文章
doi：10.1038/bjc.1990.39
更新日期：1990-02-01 00:00:00
abstract：BACKGROUND:Proviral integration Moloney virus (PIM) kinases (PIM1, 2 and 3) are overexpressed in several tumour types and contribute to oncogenesis. AZD1208 is a potent ATP-competitive PIM kinase inhibitor investigated in patients with recurrent or refractory acute myeloid leukaemia (AML) or advanced solid tumours. ME...
journal_title：British journal of cancer
pub_type： 杂志文章,多中心研究
doi：10.1038/s41416-018-0082-1
更新日期：2018-05-01 00:00:00
abstract：BACKGROUND:Heat shock protein 90 (HSP90) has a key role in the maintenance of the cellular proteostasis. However, HSP90 is also involved in stabilisation of oncogenic client proteins and facilitates oncogene addiction and cancer cell survival. The development of HSP90 inhibitors for cancer treatment is an area of growi...
journal_title：British journal of cancer
pub_type： 杂志文章
doi：10.1038/bjc.2013.710
更新日期：2014-01-07 00:00:00
abstract：BACKGROUND:Recent studies have indicated the prognostic value of tumour subtype and pathological complete response (pCR) after neoadjuvant chemotherapy (NAC). However these results were reported after a short follow-up and using a standard Cox model which could be unsatisfactory for time-dependent factors. In the prese...
journal_title：British journal of cancer
pub_type： 杂志文章
doi：10.1038/bjc.2015.174
更新日期：2015-06-30 00:00:00
abstract：BACKGROUND:Circulating total cytokeratin 18 (tCK18) and/or caspase cleaved cytokeratin 18 (cCK18) (measured by M65 and M30 enzyme-linked immunosorbent assays (ELISAs), respectively) are used as pharmacodynamic (PD) biomarkers of epithelial cell death in clinical trials. Having validated these ELISAs, we assessed their ...
journal_title：British journal of cancer
pub_type： 杂志文章
doi：10.1038/bjc.2012.416
更新日期：2012-10-23 00:00:00
abstract：:Changes in the buccal mucosa of 296 Indian and Malay betel-nut chewers in Perak, West Malaysia, were studied clinically. 167 out of 212 Indian subjects incorporated tobacco in their quids, while 45 out of 84 Malay subjects incorporated. "Gambir". The Indians appeared to show a higher proportion of mucosal changes, par...
journal_title：British journal of cancer
pub_type： 杂志文章
doi：10.1038/bjc.1970.50
更新日期：1970-09-01 00:00:00
abstract：BACKGROUND:OCT3/4 (POU5F1) is an established diagnostic immunohistochemical marker for specific histological variants of human malignant germ cell tumours (GCTs), including the seminomatous types and the stem cell component of non-seminomas, known as embryonal carcinoma. OCT3/4 is crucial for the regulation of pluripot...
journal_title：British journal of cancer
pub_type： 杂志文章
doi：10.1038/bjc.2011.270
更新日期：2011-09-06 00:00:00
abstract：:Previous studies have suggested that infant vaccinations may reduce the risk of subsequent childhood leukaemia. Vaccination histories were compared in 439 children (ages 0-14) diagnosed with acute lymphoblastic leukaemia (ALL) in nine Midwestern and Mid-Atlantic states (USA) between 1 January 1989 and 30 June 1993 and...
journal_title：British journal of cancer
pub_type： 杂志文章
doi：10.1038/sj.bjc.6690668
更新日期：1999-09-01 00:00:00
