Abstract:
BACKGROUND:Circulating total cytokeratin 18 (tCK18) and/or caspase cleaved cytokeratin 18 (cCK18) (measured by M65 and M30 enzyme-linked immunosorbent assays (ELISAs), respectively) are used as pharmacodynamic (PD) biomarkers of epithelial cell death in clinical trials. Having validated these ELISAs, we assessed their utility in colorectal cancer (CRC). METHODS:We applied the assays in several settings: 53 controls; 97 patients undergoing surgery and 74 patients with metastatic CRC undergoing chemotherapy (55 first line; 56 patients with repeated sampling through chemotherapy). Prognostic significance was evaluated using Kaplan-Meier life tables and Cox models; PD utility was assessed by analysis of repeated measures. RESULTS:Median cCK18 and tCK18 levels were elevated in patients with cancer (both P=0.0001), and among cancer patients, there were increasing trends from early to advanced stages (both P(trends)=0.0001). Increasing tCK18 predicted for reduced survival after surgery with curative intent (adjusted hazard ratio (HR) for doubling in concentration 1.77, 95% CI: 1.04, 3.01) and after first-line chemotherapy in metastatic disease (adjusted HR per doubling in concentration=1.78, 95% CI: 1.37, 2.30). In patients with progressive disease during chemotherapy, repeated sampling revealed profiles with high baselines and progressive upwardly increases after cycle 1. CONCLUSION:This study provides evidence for cytokeratin 18 (CK18) as a prognostic and PD biomarker in patients with CRC and supports continued deployment of circulating CK18 in biomarker-enhanced trials.
journal_name
Br J Cancerjournal_title
British journal of cancerauthors
Greystoke A,Dean E,Saunders MP,Cummings J,Hughes A,Ranson M,Dive C,Renehan AGdoi
10.1038/bjc.2012.416subject
Has Abstractpub_date
2012-10-23 00:00:00pages
1518-24issue
9eissn
0007-0920issn
1532-1827pii
bjc2012416journal_volume
107pub_type
杂志文章abstract::NRH:Quinone Oxidoreductase 2 (NQO2) has been described as having no enzymatic activity with nicotinamide adenine dinucleotide (NADH) or NADPH as electron donating cosubstrates. Mitomycin C (MMC) is both a substrate for and a mechanistic inhibitor of the NQO2 homologue NQO1. NRH:quinone oxidoreductase 2 catalysed the r...
journal_title:British journal of cancer
pub_type: 杂志文章
doi:10.1038/sj.bjc.6603414
更新日期:2006-11-06 00:00:00
abstract::NAD(P)H:quinone oxidoreductase (NQO1, EC 1.6.99.2) is an obligate two-electron reductase that can either bioactivate or detoxify quinones and has been proposed to play an important role in chemoprevention. We have previously characterized a homozygous point mutation in the BE human colon carcinoma cell line that leads...
journal_title:British journal of cancer
pub_type: 杂志文章
doi:10.1038/bjc.1997.11
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pub_type: 杂志文章
doi:10.1038/bjc.2016.423
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abstract:BACKGROUND:Vascular endothelial growth factor (VEGF)-mediated angiogenesis mediates tumour growth and metastasis. Meningiomas are primarily benign, slow-growing, highly vascularised tumours. Aside from VEGF, there is little data on the function of major angiogenic proteins in meningiomas. METHODS:The VEGFA, platelet-d...
journal_title:British journal of cancer
pub_type: 杂志文章
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更新日期:2012-11-06 00:00:00
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更新日期:2014-07-29 00:00:00
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journal_title:British journal of cancer
pub_type: 临床试验,杂志文章,多中心研究,随机对照试验
doi:10.1038/bjc.1998.420
更新日期:1998-01-01 00:00:00
abstract::Immunoreactive and bioassayable plasma fibronectin (opsonic α(2) surface-binding (SB) glycoprotein) was measured during experimental Sarcoma-180 tumour growth in mice. Male C57BL/6 mice were challenged s.c. with 2 × 10(6) viable Sarcoma-180 tumour cells and evaluated sequentially in parallel with saline-injected contr...
journal_title:British journal of cancer
pub_type: 杂志文章
doi:10.1038/bjc.1980.174
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journal_title:British journal of cancer
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journal_title:British journal of cancer
pub_type: 杂志文章,多中心研究
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更新日期:2004-11-29 00:00:00
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journal_title:British journal of cancer
pub_type: 杂志文章
doi:10.1038/bjc.1996.377
更新日期:1996-08-01 00:00:00
abstract::Darbepoetin alfa is a novel erythropoiesis stimulating protein (NESP), which stimulates erythropoiesis by the same mechanism as recombinant human erythropoietin (rHuEPO). NESP has been shown to be safe and efficacious in patients with chronic renal failure. NESP is biochemically distinct from rHuEPO, due to its increa...
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pub_type: 临床试验,杂志文章,多中心研究
doi:10.1054/bjoc.2001.1748
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abstract::Eleven patients with measurable subcutaneous or pulmonary metastases were selected for a study of the effectiveness of the radiosensitizer misonidazole (MIS). Evaluable data were obtained in 6 patients and radiosensitization demonstrated in 5. Patients were irradiated either before or after MIS, and each patient acted...
journal_title:British journal of cancer
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abstract::In this study we investigated the tumorigenicity, growth pattern and spontaneous metastatic ability of a series of nine human colorectal carcinoma cell lines after subcutaneous and intracaecal xenografting in nude mice. CaCo2 cells were found to be poorly tumorigenic to non-tumorigenic in either site; the other cell l...
journal_title:British journal of cancer
pub_type: 杂志文章
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更新日期:1995-02-01 00:00:00
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更新日期:2014-11-11 00:00:00
abstract::The role of the anti-apoptotic protein Bcl-2 in lung cancer remains controversial. In order to clarify its impact on survival in small and non-small cell lung cancer (NSCLC), we performed a systematic review of the literature. Trials were selected for further analysis if they provided an independent assessment of Bcl-...
journal_title:British journal of cancer
pub_type: 杂志文章,meta分析,评审
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abstract::In Vaud, Switzerland, the incidence of carcinoids based on 218 malignant and 215 benign cases rose from 19.6/10(6)in 1974-85 to 28. 2/10(6)in 1986-97, more so among males and malignant neoplasms. Lung was the commonest site for malignant and large intestine for benign carcinoids. Sixty-eight (16%) carcinoids had anoth...
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doi:10.1054/bjoc.2000.1394
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更新日期:2009-01-13 00:00:00
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journal_title:British journal of cancer
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更新日期:1973-11-01 00:00:00
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pub_type: 杂志文章,多中心研究,实务指引
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abstract::Comfrey is a rat liver toxin and carcinogen that has been used as a vegetable and herbal remedy by humans. In order to evaluate the mechanisms underlying its carcinogenicity, we examined the mutagenicity of comfrey in the transgenic Big Blue rat model. Our results indicate that comfrey is mutagenic in rat liver and th...
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