Abstract：
:Chinese hamster V79-171B spheroids implanted in the peritoneal cavity of C3H mice were characterized as a model for evaluating the toxicity of drugs requiring metabolic activation in vivo. After 24 hours in the peritoneal cavity, spheroid cellularity and plating efficiency were not significantly decreased, and host cell infiltration was estimated between 5 and 10%. The oxygenation of spheroids in the peritoneal cavity was assessed using their response to ionizing radiation. Spheroids were recovered after irradiation, incubated for 20 minutes in vitro with the slowly penetrating fluorescent dye, Hoechst 33342, and reduced to single cells with trypsin. Cells were analysed for clonogenicity as a function of position within the spheroid by using fluorescence-activated cell sorting in conjunction with the Hoechst diffusion gradient. When spheroids were first placed in the peritoneal cavity, the hypoxic fraction was close to 100%, but after 24 hours, cell oxygenation increased, probably due to a decrease in cell respiration rate. However, the location of a spheroid within the peritoneal cavity did not influence the radiosensitivity of individual spheroids or the amount of Hoechst 33342 delivered to the spheroid when Hoechst was given intravenously; individual spheroids recovered from mice given an intravenous injection of Hoechst showed no greater heterogeneity in binding than that observed when spheroids were incubated with Hoechst in vitro. Mice implanted with spheroids were also exposed to cyclophosphamide; the external cells of 0.6 mm diameter spheroids were about 30% more sensitive than the internal cells to the toxic effects of both cyclophosphamide and X-rays, and the combination of the two agents was additive at all depths within the spheroid.
journal_name
Br J Cancerjournal_title
British journal of cancerauthors
Olive PLdoi
10.1038/bjc.1987.196subject
Has Abstractpub_date
1987-09-01 00:00:00pages
321-4issue
3eissn
0007-0920issn
1532-1827journal_volume
56pub_type
杂志文章abstract：:Since hyperinsulinaemia may promote obesity-linked cancers, we compared type 2 diabetes prevalence among oesophageal adenocarcinoma (OAC) patients and population controls. Diabetes increased the risk of OAC (adjusted odds ratio 1.59, 95% confidence interval (CI) 1.04-2.43), although the risk was attenuated after furth...
journal_title：British journal of cancer
pub_type： 杂志文章
doi：10.1038/sj.bjc.6604908
更新日期：2009-03-10 00:00:00
abstract：:Two hundred and twenty-two patients with advanced breast cancer were randomised in two separate trials of similar design to either concomitant combination treatment or sequential use of the same drugs given as single agents changed only at disease progression. Both trials used cyclophosphamide, methotrexate, 5-fluorou...
journal_title：British journal of cancer
pub_type： 临床试验,杂志文章,随机对照试验
doi：10.1038/bjc.1989.46
更新日期：1989-02-01 00:00:00
abstract：:In the BUPA Study, a prospective study of 22,000 men attending a screening centre in London, serum samples were collected and stored. The concentration of beta-carotene was measured in the stored serum samples from 271 men who were subsequently notified as having cancer and from 533 unaffected controls, matched for ag...
journal_title：British journal of cancer
pub_type： 杂志文章
doi：10.1038/bjc.1988.97
更新日期：1988-04-01 00:00:00
abstract：:To investigate the efficacy and safety of combining weekly oxaliplatin with weekly 24-h infusion of high-dose 5-fluorouracil (5-FU) and folinic acid (FA) in treatment of patients with advanced gastric cancer. Patients with histologically confirmed, locally advanced or recurrent/metastatic gastric cancer were studied. ...
journal_title：British journal of cancer
pub_type： 临床试验,杂志文章,多中心研究
doi：10.1038/sj.bjc.6601985
更新日期：2004-08-02 00:00:00
abstract：:High response rates have been reported in the treatment of advanced gastric cancer with epirubicin, cisplatin and continuous infusion 5-fluorouracil (ECF), including instances of unresectable disease being rendered operable by chemotherapy. We report our experience with ECF as neoadjuvant treatment in gastric and lowe...
journal_title：British journal of cancer
pub_type： 临床试验,杂志文章
doi：10.1038/bjc.1996.604
更新日期：1996-11-01 00:00:00
abstract：:Changes in the buccal mucosa of 296 Indian and Malay betel-nut chewers in Perak, West Malaysia, were studied clinically. 167 out of 212 Indian subjects incorporated tobacco in their quids, while 45 out of 84 Malay subjects incorporated. "Gambir". The Indians appeared to show a higher proportion of mucosal changes, par...
journal_title：British journal of cancer
pub_type： 杂志文章
doi：10.1038/bjc.1970.50
更新日期：1970-09-01 00:00:00
abstract：BACKGROUND:Locally advanced oesophageal cancer (LAEC) is associated with poor survival and more effective treatments are needed. The aim of this phase I trial was to assess the maximum tolerated dose (MTD) of a novel weekly docetaxel and cisplatin regimen concurrent with radical radiotherapy. METHODS:Patients with unr...
journal_title：British journal of cancer
pub_type： 杂志文章,多中心研究
doi：10.1038/sj.bjc.6606051
更新日期：2011-01-18 00:00:00
abstract：:The incidence of B cell leukaemia in 186 consecutive untreated patients with histologically defined B cell neoplasms is described. The lymphomas were classified by the Kiel convention. B cell leukaemia in the context of this paper refers to the situation where a neoplastic clone of B cells in the blood greatly outnumb...
journal_title：British journal of cancer
pub_type： 杂志文章
doi：10.1038/bjc.1983.174
更新日期：1983-08-01 00:00:00
abstract：BACKGROUND:Several recent studies have demonstrated that microRNAs (miRNAs) are stably detectable in the plasma/serum. We hypothesised that miR-18a in the plasma is a potential biomarker in patients with pancreatic cancer. METHODS:miR-18a is located in the miR-17-92 cluster and reported to be highly expressed in pancr...
journal_title：British journal of cancer
pub_type： 杂志文章
doi：10.1038/bjc.2011.453
更新日期：2011-11-22 00:00:00
abstract：:O6-benzylguanine (O6-BG) and 3-aminobenzamide (3-AB) inhibit the DNA repair proteins O6-alkylguanine-DNA alkyltransferase (AGT) and poly(ADP-ribose) polymerase (PARP) respectively. The effect of O6-BG and/or 3-AB on temozolomide and 1,3-bis(2-chloroethyl)-nitrosourea (BCNU) cytotoxicity, was assessed in seven human tu...
journal_title：British journal of cancer
pub_type： 杂志文章
doi：10.1038/bjc.1996.485
更新日期：1996-10-01 00:00:00
abstract：:Although patients with superficial bladder cancer (Ta, T1) have a generally good prognosis, those patients who develop muscle-invasive tumours or metastatic disease at recurrence do poorly clinically. In the current study 69 patients undergoing complete transurethral resection for superficial transitional cell cancer ...
journal_title：British journal of cancer
pub_type： 杂志文章
doi：10.1038/bjc.1995.41
更新日期：1995-01-01 00:00:00
abstract：:The blood-brain barrier is a major obstacle for the chemotherapeutic drugs to effectively reach primary or secondary brain tumours. Stealth liposomal drugs are highly accumulated in tumoural tissues. In the present study we investigated the relative accumulation of(99m)Tc-DTPA radiolabelled stealth liposomal doxorubic...
journal_title：British journal of cancer
pub_type： 杂志文章
doi：10.1054/bjoc.2000.1459
更新日期：2000-11-01 00:00:00
abstract：:A brief review is presented of the evidence linking the development of certain types of neoplasms, and of the malignant lymphomas in particular, to chronic immunosuppression in animals and man and to the naturally occurring human immunologic deficiency states. The discussion then focuses on Hodgkin's disease and consi...
journal_title：British journal of cancer
pub_type： 杂志文章,评审
doi：10.1038/bjc.1971.78
更新日期：1971-12-01 00:00:00
abstract：:Identification of a biomarker of prognosis and response to therapy that can be assessed preoperatively would significantly improve overall outcomes for patients with pancreatic cancer. In this study, patients whose tumours exhibited high LMO4 expression had a significant survival advantage following operative resectio...
journal_title：British journal of cancer
pub_type： 杂志文章
doi：10.1038/sj.bjc.6604177
更新日期：2008-02-12 00:00:00
abstract：:beta2-Microglobulin (beta2M), a component of MHC class I molecules, is believed to be associated with tumour status in various cancers. In this study, we examined the expression of beta2M at different malignant stages of oral cavity squamous cell carcinoma (OCSCC). To determine the possible correlation between beta2M ...
journal_title：British journal of cancer
pub_type： 杂志文章
doi：10.1038/sj.bjc.6604698
更新日期：2008-11-04 00:00:00
abstract：:Combinations of dacarbazine (DTIC) and other cytotoxic agents or alpha-interferon were given to 136 patients in five different regimens. The total response rate was 32% (95% confidence interval 24-40%); 13% had a complete remission. Female patients had a significantly higher chance of response than male patients: 46% ...
journal_title：British journal of cancer
pub_type： 临床试验,杂志文章
doi：10.1038/bjc.1994.372
更新日期：1994-10-01 00:00:00
abstract：:Loss of the short arm of chromosome 1 is a hallmark of oligodendroglial tumours (OTs). Deletion mapping studies in OTs have revealed multiple commonly deleted regions on chromosome 1p, suggesting that there are more than one tumour suppressor gene. To map critical deletion regions on 1p, a series of 25 OTs were examin...
journal_title：British journal of cancer
pub_type： 杂志文章
doi：10.1038/sj.bjc.6602093
更新日期：2004-09-13 00:00:00
abstract：BACKGROUND:New therapeutic options for metastatic pancreatic cancer are urgently needed. In pancreatic cancer, overexpression of the epidermal growth factor receptor 2 (HER2) has been reported in up to 45%. This multicentre phase II study investigated the efficacy and toxicity of the HER2 antibody trastuzumab combined ...
journal_title：British journal of cancer
pub_type： 杂志文章,多中心研究
doi：10.1038/bjc.2012.18
更新日期：2012-03-13 00:00:00
abstract：:Germline anomalies of the INK4a-ARF and Cdk4 genes were sought in a series of 89 patients suspected of having a genetic predisposition to melanoma. Patients were selected based on the following criteria: (a) familial melanoma (23 cases), (b) multiple primary melanoma (MPM; 18 cases), (c) melanoma and additional unrela...
journal_title：British journal of cancer
pub_type： 杂志文章
doi：10.1038/sj.bjc.6601503
更新日期：2004-01-26 00:00:00
abstract：:Gain of 1q is one of the most common alterations in cancer and has been associated with adverse clinical behaviour in ependymoma. The aim of this study was to investigate this region to gain insight into the role of 1q genes in intracranial paediatric ependymoma. To address this issue we generated profiles of eleven e...
journal_title：British journal of cancer
pub_type： 杂志文章
doi：10.1038/sj.bjc.6604651
更新日期：2008-10-07 00:00:00
abstract：:Human peripheral-blood mononuclear cells, separated by Isopaque-Ficoll flotation and E-rosette formation, were tested by the fluorescein fluorescence polarization method of Cercek & Cercek (the SCM test). The response to stimulation with PHA or cancer tissue leads to a decreased polarization value TP). The responding ...
journal_title：British journal of cancer
pub_type： 杂志文章
doi：10.1038/bjc.1979.228
更新日期：1979-10-01 00:00:00
abstract：:Some cancer cells depend on the function of specific molecules for their growth, survival, and metastatic potential. Targeting of these critical molecules has arguably been the best therapy for cancer as demonstrated by the success of tamoxifen and trastuzumab in breast cancer. This review will evaluate the type I IGF...
journal_title：British journal of cancer
pub_type： 杂志文章,评审
doi：10.1038/sj.bjc.6602963
更新日期：2006-02-27 00:00:00
abstract：:Ultrafiltrates from spleen inhibited both DNA synthesis and the proliferation of normal lymphocytes stimulated inculture from both mouse and man without apparent cytotoxicity. However, the same doses of this spleen ultrafiltrate will kill up to two-thirds of the leukaemic lymphoblasts from both mouse and man after 24 ...
journal_title：British journal of cancer
pub_type： 杂志文章
doi：10.1038/bjc.1975.280
更新日期：1975-12-01 00:00:00
abstract：BACKGROUND:Period analysis is increasingly used to compute long-term cancer survival, as it provides better prediction of survival of newly diagnosed patients than traditional cohort analysis. However, the patient population to which period survival estimates best pertain to and which should be described in a study is ...
journal_title：British journal of cancer
pub_type： 杂志文章
doi：10.1038/bjc.2013.14
更新日期：2013-02-19 00:00:00
abstract：:Immunofluorescent assessment of hormone receptors in melanocytic tumours is quite feasible without loss of diagnostic material, in contrast to the impracticability of quantitative biochemical assays. Using this method, oestrogen receptors were demonstrated in 4/6, and progesterone receptors in 3/5 patients with metast...
journal_title：British journal of cancer
pub_type： 杂志文章
doi：10.1038/bjc.1981.95
更新日期：1981-05-01 00:00:00
abstract：:Growth of the transplantable EMR-86 rat mammary carcinoma depends on elevated prolactin levels which are induced by oestrogenic stimulation of the pituitary. We investigated histological and cell kinetic changes during tumour regression after removal of implanted oestrogen pellets (EP), and we especially focused on th...
journal_title：British journal of cancer
pub_type： 杂志文章
doi：10.1038/bjc.1996.232
更新日期：1996-05-01 00:00:00
abstract：:In a previous study we found that aromatic amines, particularly 4-aminobiphenyl, formed haemoglobin adducts at higher concentrations in the blood of smokers compared to non-smokers. We re-analyse here data on haemoglobin adducts of 14 aromatic amines in order to ascertain if the inter-individual variability left unexp...
journal_title：British journal of cancer
pub_type： 杂志文章
doi：10.1038/bjc.1990.120
更新日期：1990-04-01 00:00:00
abstract：BACKGROUND:The urokinase plasminogen activator (uPA) system is one of the best-investigated protease systems, both under physiological and pathological conditions, including various types of cancer. However, effects of co-expression of members of the uPA system in soft-tissue sarcoma (STS) patients at the protein level...
journal_title：British journal of cancer
pub_type： 杂志文章
doi：10.1038/sj.bjc.6605520
更新日期：2010-02-16 00:00:00
abstract：:The factors affecting long-term survival following oesophagectomy for oesophageal cancer are poorly understood. We examined the significance of microscopic tumour involvement at the circumferential resection margin (CRM) on postoperative survival following oesophagectomy. The case notes of 329 patients who underwent a...
journal_title：British journal of cancer
pub_type： 杂志文章
doi：10.1038/sj.bjc.6600931
更新日期：2003-05-19 00:00:00
abstract：:Raised risks of several cancers have been found in patients with type II diabetes, but there are few data on cancer risk in type I diabetes. We conducted a cohort study of 28 900 UK patients with insulin-treated diabetes followed for 520 517 person-years, and compared their cancer incidence and mortality with national...
journal_title：British journal of cancer
pub_type： 杂志文章
doi：10.1038/sj.bjc.6602611
更新日期：2005-06-06 00:00:00
