Abstract:
:Dolutegravir (S/GSK1349572) is a second-generation HIV-1 integrase inhibitor (INI) in advanced clinical development. It has shown good antiviral activity in most patients with prior raltegravir failure, although changes at the integrase codon 148, particularly when combined with other mutations, confer reduced susceptibility and may impair dolutegravir activity. Mutations believed to be associated with dolutegravir resistance at positions 92, 101, 124, 148, 153, and 193 were assessed in patients either INI-naïve or experiencing failure to raltegravir-based regimens. The integrase coding region was sequenced using an in-house nested-PCR protocol. HIV-1 subtyping was carried out using the Stanford algorithm. A total of 638 plasma samples were analyzed from 535 INI-naïve and 103 raltegravir-experienced patients. Non-B subtypes were recognized in 20.8% patients. Mutations L101I and T124A were significantly more prevalent in patients with non-B subtypes (66.9% vs. 45.7% for L101I; 61.7% vs. 25.9% for T124A; and 39.1% vs. 12.7% for L101I+T124A; p<0.001 in all cases). E92Q and Q148H/R were only seen in raltegravir-experienced patients and exclusively infected with subtype B (1.9% vs. 0%, p=0.026, for E92Q and 12.6% vs. 0%, p<0.001, for Q148H/R). On the contrary, T124A was more frequent in INI-naïve than raltegravir-experienced patients (35.1% vs. 24.3%, p=0.040). S153Y/F was absent in this dataset. Polymorphic changes L101I and T124A were more frequent in HIV-1 non-B than B subtypes. T124A was more frequent in INI-naïve patients but E92Q and Q148H/R were only seen in raltegravir-experienced individuals. Thus, both HIV-1 subtype and raltegravir exposure may influence the antiviral activity of dolutegravir.
journal_name
Antiviral Resjournal_title
Antiviral researchauthors
Garrido C,Soriano V,Geretti AM,Zahonero N,Garcia S,Booth C,Gutierrez F,Viciana I,de Mendoza Cdoi
10.1016/j.antiviral.2011.03.178subject
Has Abstractpub_date
2011-06-01 00:00:00pages
164-7issue
3eissn
0166-3542issn
1872-9096pii
S0166-3542(11)00230-0journal_volume
90pub_type
杂志文章abstract::Lumpy skin disease is of significant economic impact for the cattle industry in Africa. The disease is currently spreading aggressively in the Near East, posing a threat of incursion to Europe and Asia. Due to cross-protection within the Capripoxvirus genus, sheep pox virus (SPPV) vaccines have been widely used for ca...
journal_title:Antiviral research
pub_type: 杂志文章
doi:10.1016/j.antiviral.2014.06.009
更新日期:2014-09-01 00:00:00
abstract::Preventive intraperitoneal trehalose dimycolate (TDM) treatment of mice, inoculated with encephalomyocarditis (EMC) virus by the same route, caused restriction of virus growth in the peritoneum, which was correlated to IFN production in peritoneal fluids prior to infection. Peritoneal macrophages from TDM-treated mice...
journal_title:Antiviral research
pub_type: 杂志文章
doi:10.1016/0166-3542(95)00047-p
更新日期:1995-10-01 00:00:00
abstract::Oligodeoxynucleotides (ODN) containing unmethylated CpG motifs are potent stimulators of the innate immune system, and are capable of aborting several infections in a non-specific manner. We here report studies of the capacity of such ODN to protect mice against infection with foot and mouth disease virus (FMDV). Susc...
journal_title:Antiviral research
pub_type: 杂志文章
doi:10.1016/j.antiviral.2006.03.010
更新日期:2006-10-01 00:00:00
abstract::Human adenoviruses (HAdVs) usually cause mild respiratory infections, but they can also lead to fatal outcomes for immunosuppressive patients. Unfortunately, there has been no specific anti-HAdV drug approved for medical use. A better understanding of the nature of virus-host interactions during infection is beneficia...
journal_title:Antiviral research
pub_type: 杂志文章
doi:10.1016/j.antiviral.2018.01.014
更新日期:2018-04-01 00:00:00
abstract::We studied susceptibility to herpes labialis by exposing 20 volunteers to experimental ultraviolet radiation (UVR) on three occasions at 3- to 4-month intervals. The number of patients who developed lesions after each session was 9/20 (45%), 9/20 (45%) and 14/20 (70%). Herpes simplex virus (HSV) was isolated from 21/2...
journal_title:Antiviral research
pub_type: 杂志文章
doi:10.1016/0166-3542(95)00038-n
更新日期:1995-09-01 00:00:00
abstract::The effects of HIV-1 protease inhibitors on proteolytic processing and infectivity of virions produced from lymphocytes chronically infected with the virus were studied. Protease inhibition was detected by the accumulation of the polyprotein precursors Pr55gag and Pr160gag-pol and their cleavage intermediates. Immunob...
journal_title:Antiviral research
pub_type: 杂志文章
doi:10.1016/s0166-3542(99)00074-1
更新日期:2000-01-01 00:00:00
abstract::Resveratrol (RV), a polyphenolic natural product present in many plants and fruits, exhibits anti-inflammatory, cardio-protective and anti-proliferative properties. Moreover, RV affects a wide variety of viruses including members of the Herpesviridae family, retroviruses, influenza A virus and polyomavirus by altering...
journal_title:Antiviral research
pub_type: 杂志文章
doi:10.1016/j.antiviral.2012.09.003
更新日期:2012-11-01 00:00:00
abstract::The antiviral effect of Hochu-ekki-to (TJ-41), a Japanese herbal medicine, was investigated using mice infected with influenza virus. TJ-41 was found to increase the survival rate, prolong the mean survival days, suppress viral growth in bronchoalveolar labage fluid (BALF) and inhibit the lung index (lung consolidatio...
journal_title:Antiviral research
pub_type: 杂志文章
doi:10.1016/s0166-3542(99)00048-0
更新日期:1999-12-15 00:00:00
abstract::Ribonucleases H (RNases H) are endonucleolytic enzymes, evolutionarily related to retroviral integrases, DNA transposases, resolvases and numerous nucleases. RNases H cleave RNA in RNA/DNA hybrids and their activity plays an important role in the replication of prokaryotic and eukaryotic genomes, as well as in the rep...
journal_title:Antiviral research
pub_type: 杂志文章,评审
doi:10.1016/j.antiviral.2019.104613
更新日期:2019-11-01 00:00:00
abstract::While the combination of zidovudine and didanosine is used in HIV-infected patients with more advanced disease, the possibility of a pharmacokinetic interaction between these two drugs remains controversial. Zidovudine doses of 50, 100, and 200 mg, combined with 67, 167, and 250 mg of didanosine were evaluated in 11 a...
journal_title:Antiviral research
pub_type: 临床试验,杂志文章,随机对照试验
doi:10.1016/0166-3542(95)00032-h
更新日期:1995-08-01 00:00:00
abstract::Childhood exanthema caused by different serotypes of coxsackievirus (CV-A) and enterovirus A71 (EV-A71) has become a serious global health problem; it is commonly known as hand, foot, and mouth disease (HFMD). Current EV-A71 vaccine clinical trials have demonstrated that human antibody responses generated by EV-A71 va...
journal_title:Antiviral research
pub_type: 杂志文章
doi:10.1016/j.antiviral.2016.02.008
更新日期:2016-05-01 00:00:00
abstract::In March and April 2009, an entirely novel influenza A (H1N1) virus (NIAV) emerged in Mexico and the USA. During the subsequent months, the virus rapidly spread all over the world by person-to-person transmission. In this report, RNA interference (RNAi) was used as an antiviral agent to inhibit NIAV replication in A54...
journal_title:Antiviral research
pub_type: 杂志文章
doi:10.1016/j.antiviral.2009.12.010
更新日期:2010-03-01 00:00:00
abstract::Parvovirus B19 (B19V), a single-stranded DNA virus in the family Parvoviridae, is a human pathogenic virus responsible for a wide range of clinical manifestations. Currently there is no approved antiviral therapy for parvovirus infection. The acyclic nucleoside phosphonate cidofovir (CDV) has been demonstrated to inhi...
journal_title:Antiviral research
pub_type: 杂志文章
doi:10.1016/j.antiviral.2018.12.003
更新日期:2019-02-01 00:00:00
abstract::Human CMV causes a number of diseases that cause considerable morbidity and that can be life-threatening in immunocompromised patients, particularly those with AIDS. Ganciclovir (GCV) and Foscarnet (PFA) are currently the drugs of choice for management of CMV disease. Both are not without side effects and have a relat...
journal_title:Antiviral research
pub_type: 杂志文章
doi:10.1016/0166-3542(94)90048-5
更新日期:1994-05-01 00:00:00
abstract::Interferon alpha-2b (IFN) alone or in combination with Ribavirin is approved in the United States for the treatment of chronic hepatitis C virus (HCV) infection. We have previously reported that the glucosidase inhibitor, n-butyl deoxynojirimycin (nB-DNJ) inhibits the production of infectious bovine diarrhea virus (BV...
journal_title:Antiviral research
pub_type: 杂志文章
doi:10.1016/s0166-3542(02)00075-x
更新日期:2002-09-01 00:00:00
abstract::Dengue virus (DENV) is the most prevalent mosquito borne viral pathogen worldwide. In this work we first evaluated the antiviral activity of natural and synthetic β-carbolines against DENV-2 multiplication in cell cultures. We determined that the natural β-carboline harmol and a synthetic harmine derivative, 9N-methyl...
journal_title:Antiviral research
pub_type: 杂志文章
doi:10.1016/j.antiviral.2016.08.018
更新日期:2016-10-01 00:00:00
abstract::In the absence of antiviral treatment, chronic hepatitis C virus (HCV) infection is a liver disease characterized by the development of necroinflammatory changes and progressive liver fibrosis, leading to cirrhosis, end-stage liver disease and hepatocellular carcinoma (HCC). The approval of ribavirin in combination th...
journal_title:Antiviral research
pub_type: 杂志文章,评审
doi:10.1016/j.antiviral.2003.08.009
更新日期:2003-10-01 00:00:00
abstract::Global eradication of hepatitis C virus (HCV) infection will require an efficacious vaccine capable of eliciting protective immunity against genetically diverse HCV strains. Natural spontaneous resolution of HCV infection is associated with production of broadly-neutralizing antibodies targeting the HCV glycoproteins ...
journal_title:Antiviral research
pub_type: 杂志文章
doi:10.1016/j.antiviral.2018.09.005
更新日期:2018-12-01 00:00:00
abstract::Cytomegalovirus (CMV) is one of the most common opportunistic infections after transplantation. To prevent CMV infections, universal prophylaxis and pre-emptive therapy with ganciclovir or its prodrug valganciclovir is applied. However, prolonged antiviral therapy may result in drug-resistance emergence. We describe a...
journal_title:Antiviral research
pub_type: 杂志文章
doi:10.1016/j.antiviral.2019.06.004
更新日期:2019-08-01 00:00:00
abstract::This study aimed to investigate anti-HBV effect and major active compounds of Su-duxing, a medicine extracted from Chinese herbs. HBV-replicating cell lines HepG2.2.15 (wild-type) and HepG2.A64 (entecavir-resistant) were used for in vitro test. C57BL/6 mice infected by adeno-associated virus carrying 1.3 mer wild-type...
journal_title:Antiviral research
pub_type: 杂志文章
doi:10.1016/j.antiviral.2018.04.017
更新日期:2018-07-01 00:00:00
abstract::In the event of an influenza pandemic, the use of oseltamivir (OTV) will undoubtedly increase and therefore it is more likely that OTV-resistant influenza strains will also arise. OTV-resistance genotyping using sequence-based testing on viruses isolated in cell culture is time consuming and less likely to detect the ...
journal_title:Antiviral research
pub_type: 杂志文章
doi:10.1016/j.antiviral.2009.09.010
更新日期:2009-12-01 00:00:00
abstract::Japanese encephalitis virus (JEV) infection is a major cause of acute viral encephalitis both in humans and animals. The domain III of virus envelope protein (EDIII) plays important roles in interacting with host cell receptors to facilitate virus entry. In this study, recombinant JEV EDIII was expressed and purified....
journal_title:Antiviral research
pub_type: 杂志文章
doi:10.1016/j.antiviral.2012.03.002
更新日期:2012-05-01 00:00:00
abstract::This mini-review describes the structure-activity relationships of several series of new nucleoside analogues. It also points to the possibilities of finding new molecular modifications which could lead to antivirals with activity both against human immunodeficiency viruses (HIV) and herpesviruses. ...
journal_title:Antiviral research
pub_type: 杂志文章,评审
doi:10.1016/0166-3542(92)90052-7
更新日期:1992-07-01 00:00:00
abstract::Approximately 170 million people worldwide are chronic carriers of Hepatitis C virus (HCV). To date, there is no prophylactic vaccine available against HCV. The standard-of-care therapy for HCV infection involves a combination of pegylated interferon-α and ribavirin. This therapy, which is commonly associated with sid...
journal_title:Antiviral research
pub_type: 杂志文章,评审
doi:10.1016/j.antiviral.2012.07.006
更新日期:2012-10-01 00:00:00
abstract::Chikungunya virus (CHIKV) is an arbovirus transmitted to humans by mosquito bite. A decade ago, the virus caused a major outbreak in the islands of the Indian Ocean, then reached India and Southeast Asia. More recently, CHIKV has emerged in the Americas, first reaching the Caribbean and now extending to Central, South...
journal_title:Antiviral research
pub_type: 杂志文章,评审
doi:10.1016/j.antiviral.2015.07.002
更新日期:2015-09-01 00:00:00
abstract::While combination antiretroviral therapy (cART) has successfully converted HIV to a chronic but manageable infection in many parts of the world, HIV continues to persist within latent cellular reservoirs, which can become reactivated at any time to produce infectious virus. New therapies are therefore needed not only ...
journal_title:Antiviral research
pub_type: 杂志文章,评审
doi:10.1016/j.antiviral.2018.07.016
更新日期:2018-10-01 00:00:00
abstract::Herpesviruses encode a protease that is essential for virus replication. The protease undergoes cleavage to a processed form during capsid maturation. A recombinant 75 kDa form of the protease from human cytomegalovirus was purified and compared with the recombinant 29 kDa processed form. Modification with an active s...
journal_title:Antiviral research
pub_type: 杂志文章
doi:10.1016/s0166-3542(02)00051-7
更新日期:2002-08-01 00:00:00
abstract::The identification of more effective and less toxic foscarnet (PFA) analogs for antiviral therapy would be useful. We recently synthesized 1-O-octadecyl-sn-glycero-3-phosphonoformic acid (ODG-PFA) and noted a 93-fold increase in its anti-HCMV activity relative to PFA. In addition, the antiviral activity of ODG-PFA in ...
journal_title:Antiviral research
pub_type: 杂志文章
doi:10.1016/s0166-3542(97)00039-9
更新日期:1997-09-01 00:00:00
abstract::Hepatitis C virus (HCV) translation is mediated by a highly conserved internal ribosome entry site (IRES), mainly located at the 5'untranslatable region (5'UTR) of the viral genome. Viral protein synthesis clearly differs from that used by most cellular mRNAs, rendering the IRES an attractive target for novel antivira...
journal_title:Antiviral research
pub_type: 杂志文章
doi:10.1016/j.antiviral.2012.02.015
更新日期:2012-05-01 00:00:00
abstract::Almost 30 years ago it was proposed that the selection for antiviral drug resistance should be used as an indicator of antiviral drug activity. In addition to discriminating between cellular toxicity and specific activity directed against a viral target, drug resistant mutants have been used to confirm the mechanism o...
journal_title:Antiviral research
pub_type: 杂志文章,评审
doi:10.1016/j.antiviral.2006.03.004
更新日期:2006-09-01 00:00:00