Abstract:
:The effects of HIV-1 protease inhibitors on proteolytic processing and infectivity of virions produced from lymphocytes chronically infected with the virus were studied. Protease inhibition was detected by the accumulation of the polyprotein precursors Pr55gag and Pr160gag-pol and their cleavage intermediates. Immunoblot analysis demonstrated that while the processing of Pr55gag was largely irreversible, cleavage of Pr160gag-pol proceeded once the inhibitor was removed, although it was not completed during 96 h of subsequent observation. Virions produced during exposure of cells to protease inhibitors regained some degree of infectivity post-withdrawal of the inhibitor, suggesting that the processing of Pr160gag-pol following drug withdrawal resulted in the production of those enzymes necessary to enable at least limited viral replication. When cells were exposed to a protease inhibitor for 72 h then the inhibitor withdrawn, a lag phase of up to 24 h occurred before these cells produced virions with equivalent infectivity to virus produced from cells not exposed to drug. These observations may reflect a clinical situation likely to occur as trough plasma concentrations of protease inhibitors fall below the IC100 for HIV, highlighting the need for adherence to drug regimens containing these inhibitors.
journal_name
Antiviral Resjournal_title
Antiviral researchauthors
Jardine DK,Tyssen DP,Birch CJdoi
10.1016/s0166-3542(99)00074-1subject
Has Abstractpub_date
2000-01-01 00:00:00pages
59-68issue
1eissn
0166-3542issn
1872-9096pii
S0166-3542(99)00074-1journal_volume
45pub_type
杂志文章abstract::Combinations of anti-HIV agents including one or two reverse transcriptase inhibitors with a protease inhibitor are potent and effective. However, toxicities, costs and the emergence of drug-resistant organisms have compromised their long-term efficacy in people. A next, likely, target for anti-HIV therapy is HIV-1 in...
journal_title:Antiviral research
pub_type: 杂志文章
doi:10.1016/s0166-3542(00)00083-8
更新日期:2000-06-01 00:00:00
abstract::Various anthraquinones substituted with hydroxyl, amino, halogen, carboxylic acid, substituted aromatic group, and sulfonate were tested to determine their activity against human immunodeficiency virus type 1 (HIV-1) in primary human lymphocytes. Among the compounds tested, polyphenolic and/or polysulfonate substitute...
journal_title:Antiviral research
pub_type: 杂志文章
doi:10.1016/0166-3542(90)90071-e
更新日期:1990-05-01 00:00:00
abstract::Several nucleoside reverse transcriptase inhibitors are associated with mitochondrial toxicity resulting from inhibition of DNA polymerase-gamma. This study compared the effects on mitochondrial DNA of apricitabine (previously referred to as AVX754 or SPD754), a novel cytidine analogue under development for the treatm...
journal_title:Antiviral research
pub_type: 杂志文章
doi:10.1016/j.antiviral.2007.05.004
更新日期:2007-10-01 00:00:00
abstract::Salvage therapy with ritonavir (RTV) and saquinavir (SQV) failed to achieve virological and immunological improvement in 24 HIV-infected patients who discontinued triple therapy with RTV or indinavir (IDV) because of failure or intolerance to treatment. Changes in the HIV-1 protease gene sequence were analyzed prospec...
journal_title:Antiviral research
pub_type: 临床试验,杂志文章
doi:10.1016/s0166-3542(00)00110-8
更新日期:2000-09-01 00:00:00
abstract::Cathelicidin antimicrobial peptides (human LL-37 and mouse CRAMP) are mainly virucidal to enveloped virus. However, the effects and relative mechanisms of LL-37 and CRAMP on non-enveloped virus are elusive. We herein found that CRAMP expression was significantly up-regulated post non-enveloped Enterovirus 71 (EV71) in...
journal_title:Antiviral research
pub_type: 杂志文章
doi:10.1016/j.antiviral.2021.105021
更新日期:2021-01-25 00:00:00
abstract::Cytomegalovirus (CMV) is the most common cause of congenital infection in the developed world and can lead to a life-threatening disease. We therefore developed an animal model to evaluate candidate anti-CMV drugs and to further define the pathogenesis of CMV infections. Newborn guinea pigs were infected by intraperit...
journal_title:Antiviral research
pub_type: 杂志文章
doi:10.1016/s0166-3542(03)00151-7
更新日期:2003-09-01 00:00:00
abstract::Efavirenz (EFV), a first generation non-nucleoside analogue reverse transcriptase inhibitor widely employed in combination antiretroviral therapy regimens over the last 20 years, has been associated with a wide range of neuropsychiatric effects and has also been linked with HIV-associated neurocognitive disorder (HAND...
journal_title:Antiviral research
pub_type: 杂志文章
doi:10.1016/j.antiviral.2019.04.015
更新日期:2019-08-01 00:00:00
abstract::Varicella-zoster virus (VZV) replicates in quiescent T cells, neurons, and skin cells. In cultured fibroblasts (HFFs), VZV induces host cyclin expression and cyclin-dependent kinase (CDK) activity without causing cell cycle progression. CDK1/cyclin B1 phosphorylates the major viral transactivator, and the CDK inhibito...
journal_title:Antiviral research
pub_type: 杂志文章
doi:10.1016/j.antiviral.2010.03.007
更新日期:2010-06-01 00:00:00
abstract::The Cytomegalovirus Retinitis Retreatment Trial was a multicenter clinical trial designed to evaluate three treatments for the treatment of relapsed CMV retinitis; (1) foscarnet; (2) 'high-dose' ganciclovir, and (3) combination foscarnet and ganciclovir. Two hundred seventy-nine patients were enrolled and randomly ass...
journal_title:Antiviral research
pub_type: 临床试验,杂志文章,随机对照试验
doi:10.1016/0166-3542(95)00921-3
更新日期:1996-01-01 00:00:00
abstract::Lactoferrin (LF), a member of the transferrin family, is a bi-globular protein secreted in milk, saliva, tears, seminal fluid, endocervix and vaginal secretions. LF is an important player in the defence against pathogenic microorganisms and has also been shown to have activity against several viruses including herpesv...
journal_title:Antiviral research
pub_type: 杂志文章
doi:10.1016/j.antiviral.2004.05.005
更新日期:2004-10-01 00:00:00
abstract::An immunogenic component was isolated from both the Illinois (propagated in young swine) and the Miller (cell culture-adapted) strains of porcine transmissible gastroenteritis (TGE) virus. The viral subunit was released from the virion by sonication and was separated from intact virus and other viral components by iso...
journal_title:Antiviral research
pub_type: 杂志文章
doi:10.1016/0166-3542(83)90027-x
更新日期:1983-09-01 00:00:00
abstract::In an ongoing effort to identify an orally bioavailable compound for the treatment of rhino- and enteroviral infections, a series of vinylacetylene benzimidazoles was recently examined. Previous studies demonstrated the potential for these compounds to possess both good in vitro antiviral activity as well as acceptabl...
journal_title:Antiviral research
pub_type: 杂志文章
doi:10.1016/s0166-3542(99)00013-3
更新日期:1999-05-01 00:00:00
abstract::Sequences from the gag, pol and rev regions of the RF strain of HIV-1 (HIV-1RF) were chosen as targets for antisense phosphorothioate oligodeoxynucleotides (S-oligos). These sequences were the p18/p24 junction in gag, the active site of HIV protease in pol; a sequence from the first exon of the rev gene and S-oligodeo...
journal_title:Antiviral research
pub_type: 杂志文章
doi:10.1016/0166-3542(92)90090-r
更新日期:1992-01-01 00:00:00
abstract::Foot and mouth disease (FMD) is a highly contagious viral disease of cloven-hoofed animals. Previously, we found that the epitope peptide EP141-160 displayed on virus-like particles (VLP) for use as a vaccine showed high immunoreactivity and conferred partially effective protection to animals. In this study, we first ...
journal_title:Antiviral research
pub_type: 杂志文章
doi:10.1016/j.antiviral.2016.08.020
更新日期:2016-10-01 00:00:00
abstract::Prostratin, a non-tumor-promoting phorbol ester, inhibited human immunodeficiency virus (HIV)-induced cell killing and viral replication in a variety of acutely-infected cell systems. The potency and degree of cytoprotection was dependent on both viral strain and host cell type. Prostratin activated viral expression i...
journal_title:Antiviral research
pub_type: 杂志文章
doi:10.1016/s0166-3542(96)01004-2
更新日期:1997-01-01 00:00:00
abstract::The antiviral activity of antisense oligonucleotides corresponding to different regions of foot-and-mouth disease virus (FMDV) genome has been assessed in BHK-21 cells. The locations of the oligonucleotides used were: (i) two regions within the internal ribosome entry site (IRES), involved in the regulation of the tra...
journal_title:Antiviral research
pub_type: 杂志文章
doi:10.1016/0166-3542(93)90082-t
更新日期:1993-09-01 00:00:00
abstract::Ebola virus (EBOV), a negative-sense RNA virus in the family Filoviridae, is known to cause severe hemorrhagic fever in humans and other primates. Infection with EBOV causes a high mortality rate and currently there is no FDA-licensed vaccine or therapeutic treatment available. Recently, heat-shock protein 90 (Hsp90),...
journal_title:Antiviral research
pub_type: 杂志文章
doi:10.1016/j.antiviral.2010.04.015
更新日期:2010-08-01 00:00:00
abstract::Nalidixic acid and oxolinic acid, two antibacterial agents known to inhibit bacterial DNA gyrase, are shown to suppress the replication, as well as the cytopathic effect, of BK virus in Vero cell cultures. The inhibition of virus replication was detectable at day 4 post infection in cultures which had been continuousl...
journal_title:Antiviral research
pub_type: 杂志文章
doi:10.1016/0166-3542(88)90004-6
更新日期:1988-04-01 00:00:00
abstract::Based on genome-scale loss-of-function screens we discovered that Topoisomerase III-β (TOP3B), a human topoisomerase that acts on DNA and RNA, is required for yellow fever virus and dengue virus-2 replication. Remarkably, we found that TOP3B is required for efficient replication of all positive-sense-single stranded R...
journal_title:Antiviral research
pub_type: 杂志文章
doi:10.1016/j.antiviral.2020.104874
更新日期:2020-10-01 00:00:00
abstract::AIDS therapies employing HIV protease inhibitors (PIs) are associated with changes in fat metabolism. However, the cellular mechanisms affected by PIs are not clear. Thus, the affects of PIs on adipocyte differentiation were examined in vitro using C3H10T1/2 stem cells. In these cells the PIs, nelfinavir, saquinavir, ...
journal_title:Antiviral research
pub_type: 杂志文章
doi:10.1016/s0166-3542(00)00102-9
更新日期:2000-08-01 00:00:00
abstract::Infection by one of the 4 distinct serotypes of dengue virus (DENV) threatens >40% of the world's population, with no efficacious vaccine or antiviral agent currently available. DENV replication through the virus-encoded nonstructural protein (NS) 5 protein occurs in the infected cell cytoplasm, but NS5 from DENV2 has...
journal_title:Antiviral research
pub_type: 杂志文章
doi:10.1016/j.antiviral.2013.06.002
更新日期:2013-09-01 00:00:00
abstract::Yellow fever virus (YFV), a member of the genus Flavivirus, is a mosquito-borne virus found in tropical regions of Africa and South America that causes severe hepatic disease and death in humans. Despite the availability of effective vaccines, YFV is responsible for an estimated 200,000 cases and 30,000 deaths annuall...
journal_title:Antiviral research
pub_type: 杂志文章
doi:10.1016/j.antiviral.2012.02.001
更新日期:2012-04-01 00:00:00
abstract::Human T-cell lymphotropic virus type 1 (HTLV-1)-associated adult T-cell leukemia/lymphoma (ATLL) has a poor prognosis owing to its intrinsic resistance to chemotherapy. Although zidovudine (AZT) and alpha interferon (IFN-alpha) give rise to some response and improve the prognosis of ATLL, alternative therapies are nee...
journal_title:Antiviral research
pub_type: 杂志文章
doi:10.1016/j.antiviral.2006.02.003
更新日期:2006-07-01 00:00:00
abstract::The rapid spread of Zika virus (ZIKV) in recent years has highlighted the severe diseases associated with ZIKV infection, such as Guillain-Barré syndrome in adults and microcephaly in newborns; yet no vaccines or antivirals currently exist to prevent or treat ZIKV infection. We and others have previously identified N-...
journal_title:Antiviral research
pub_type: 杂志文章
doi:10.1016/j.antiviral.2017.10.014
更新日期:2017-11-01 00:00:00
abstract::In cultured mammalian cells (Vero), different antiviral agents change to differing degrees the ability of HSV2 to down-regulate gap junctions, each agent having a specific effect. Measured by intracellular electrodes, control cell populations showed 49-51% coupling, uninfected populations treated with acyclovir or SDS...
journal_title:Antiviral research
pub_type: 杂志文章
doi:10.1016/s0166-3542(02)00106-7
更新日期:2002-11-01 00:00:00
abstract::A screening for inhibitors of human immunodeficiency virus type 1 (HIV-1) among various types of isopolyoxomolybdates and heteropolyoxomolybdates was carried out by using an in vitro assay system measuring the cytopathogenicity of HIV-1 in CD4+ human MT-4 cells. A novel heteropolyoxomolybdate named PM-104 with the che...
journal_title:Antiviral research
pub_type: 杂志文章
doi:10.1016/0166-3542(93)90075-t
更新日期:1993-04-01 00:00:00
abstract::Cellular immune responses (T cell responses) during hepatitis C virus (HCV) infection are significant factors for determining the outcome of infection. HCV adapts to host immune responses by inducing mutations in its genome at specific sites that are important for HLA processing/presentation. Moreover, HCV also adapts...
journal_title:Antiviral research
pub_type: 杂志文章
doi:10.1016/j.antiviral.2016.10.013
更新日期:2017-01-01 00:00:00
abstract::SAMHD1 is a triphosphohydrolase that restricts HIV-1 by limiting the intracellular dNTP pool required for reverse transcription. Although SAMHD1 is expressed and active/unphosphorylated in most cell lines, its restriction activity is thought to be relevant only in non-cycling cells. However, an in depth evaluation of ...
journal_title:Antiviral research
pub_type: 杂志文章
doi:10.1016/j.antiviral.2017.03.019
更新日期:2017-06-01 00:00:00
abstract:BACKGROUND/AIMS:Traditional Chinese herbal therapies are widely used for the treatment of chronic hepatitis C (CHC) in Asia. The aim of this study was to perform a meta-analysis of randomised controlled trials (RCTs) comparing interferon therapies with Chinese herbal therapies and/or interferon plus Chinese herb therap...
journal_title:Antiviral research
pub_type: 杂志文章,meta分析
doi:10.1016/j.antiviral.2010.12.003
更新日期:2011-02-01 00:00:00
abstract::One of the plant extracts, glycyrrhizin (GL) was investigated for its antiviral action on varicella-zoster virus (VZV) in vitro. When human embryonic fibroblast (HEF) cells were treated with GL after inoculation of virus (post-treatment), the average 50%-inhibitory dose (ID50) for five VZV strains was 0.71 mM, and the...
journal_title:Antiviral research
pub_type: 杂志文章
doi:10.1016/0166-3542(87)90025-8
更新日期:1987-02-01 00:00:00