Abstract:
:Nalidixic acid and oxolinic acid, two antibacterial agents known to inhibit bacterial DNA gyrase, are shown to suppress the replication, as well as the cytopathic effect, of BK virus in Vero cell cultures. The inhibition of virus replication was detectable at day 4 post infection in cultures which had been continuously exposed to drugs at concentrations as low as 0.02 to 0.04 mM of nalidixic acid and 0.2 mM of oxolinic acid. These active concentrations are inferior to plasma levels attained in the course of clinical use of the drugs for antibacterial chemotherapy. Also, under these circumstances, no cytotoxicity occurred. The inhibition of development of cytopathology and of virus-induced cell death was demonstrable in cultures treated for 12 days with the drugs. Under these circumstances of prolonged action, oxolinic acid proved to be slightly cytotoxic in that virus inhibitory doses reduced the viability of normal cells. No alterations in the topological conformation of the viral genome or accumulation of end products of viral DNA replication were detected. However, accumulation of viral DNA form I at 48 h post infection suggests that the drugs act through a mechanism involving DNA topoisomerase.
journal_name
Antiviral Resjournal_title
Antiviral researchauthors
Portolani M,Pietrosemoli P,Cermelli C,Mannini-Palenzona A,Grossi MP,Paolini L,Barbanti-Brodano Gdoi
10.1016/0166-3542(88)90004-6subject
Has Abstractpub_date
1988-04-01 00:00:00pages
205-18issue
3eissn
0166-3542issn
1872-9096pii
0166-3542(88)90004-6journal_volume
9pub_type
杂志文章abstract::Lentiviral vectors have been shown to be good candidates for gene transfer protocols; however, prevention of insertional mutagenesis remains problematic. Here we report on the design of a conditionally replicating integrase (IN)-defective lentiviral-hybrid episomal vector in which the insertion of the SV40 promoter/or...
journal_title:Antiviral research
pub_type: 杂志文章
doi:10.1016/j.antiviral.2008.06.015
更新日期:2008-12-01 00:00:00
abstract::HIV-host infection systems in vitro are important in the pre-clinical assessment of anti-retroviral drug activity. The present report describes the development of a new HIV-host model comprised of an epithelial cell line of HeLa lineage (HeLa-1), transfected with expression vectors bearing tat and rev (TART) genes of ...
journal_title:Antiviral research
pub_type: 杂志文章
doi:10.1016/s0166-3542(99)00014-5
更新日期:1999-07-01 00:00:00
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journal_title:Antiviral research
pub_type: 杂志文章
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更新日期:2003-09-01 00:00:00
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journal_title:Antiviral research
pub_type: 临床试验,杂志文章,随机对照试验
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更新日期:1989-10-01 00:00:00
abstract::Conventional treatment of severe viral disease is limited by the narrow choice as well as the often-significant side effects or lack of clear efficacy of antiviral chemotherapy. At the same time, however, it is known that a reduction in viral load leads to significant clinical improvement in a number of important vira...
journal_title:Antiviral research
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doi:10.1016/j.antiviral.2010.05.007
更新日期:2010-08-01 00:00:00
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journal_title:Antiviral research
pub_type: 杂志文章
doi:10.1016/0166-3542(91)90040-x
更新日期:1991-01-01 00:00:00
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journal_title:Antiviral research
pub_type: 杂志文章
doi:10.1016/j.antiviral.2013.08.004
更新日期:2013-10-01 00:00:00
abstract:BACKGROUND:By modulating the antiviral immune response via vitamin D receptor, the active form of vitamin D (1,25-dihydroxyvitamin D, calcitriol) could play a central role in protection against respiratory virus infections. This in vitro study tested the hypothesis that respiratory viruses modulate vitamin D receptor e...
journal_title:Antiviral research
pub_type: 杂志文章
doi:10.1016/j.antiviral.2016.11.004
更新日期:2017-01-01 00:00:00
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journal_title:Antiviral research
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更新日期:2010-12-01 00:00:00
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journal_title:Antiviral research
pub_type: 杂志文章
doi:10.1016/s0166-3542(00)00102-9
更新日期:2000-08-01 00:00:00
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journal_title:Antiviral research
pub_type: 临床试验,杂志文章,多中心研究
doi:10.1016/j.antiviral.2011.07.003
更新日期:2011-10-01 00:00:00
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journal_title:Antiviral research
pub_type: 杂志文章
doi:10.1016/j.antiviral.2007.02.004
更新日期:2007-08-01 00:00:00
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journal_title:Antiviral research
pub_type: 杂志文章
doi:10.1016/0166-3542(82)90028-6
更新日期:1982-05-01 00:00:00
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journal_title:Antiviral research
pub_type: 杂志文章,评审
doi:10.1016/j.antiviral.2003.08.011
更新日期:2003-10-01 00:00:00
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journal_title:Antiviral research
pub_type: 杂志文章
doi:10.1016/j.antiviral.2004.12.007
更新日期:2005-04-01 00:00:00
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journal_title:Antiviral research
pub_type: 杂志文章
doi:10.1016/0166-3542(93)90093-x
更新日期:1993-10-01 00:00:00
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journal_title:Antiviral research
pub_type: 杂志文章
doi:10.1016/j.antiviral.2008.08.008
更新日期:2009-01-01 00:00:00
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journal_title:Antiviral research
pub_type: 历史文章,杂志文章,评审
doi:10.1016/j.antiviral.2015.12.003
更新日期:2016-02-01 00:00:00
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pub_type: 杂志文章
doi:10.1016/j.antiviral.2005.06.004
更新日期:2005-10-01 00:00:00
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更新日期:2021-01-25 00:00:00
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journal_title:Antiviral research
pub_type: 杂志文章
doi:10.1016/j.antiviral.2017.11.003
更新日期:2018-01-01 00:00:00
abstract::Prevention of HIV infection and unintended pregnancies are public health priorities. In sub-Saharan Africa, where HIV prevalence is highest, depot medroxyprogesterone acetate (DMPA) is widely used as contraception. Therefore, understanding potential interactions between DMPA and antiretrovirals is critical. Here, we u...
journal_title:Antiviral research
pub_type: 杂志文章
doi:10.1016/j.antiviral.2020.105001
更新日期:2020-12-29 00:00:00
abstract::The era of antiviral chemotherapy started more than 50 years with the findings by Domagk and his colleagues that thiosemicarbazones showed activity against vaccinia virus. One of the derivatives, methisazone, was even investigated in the prophylaxis of smallpox. With the successful implementation of the smallpox vacci...
journal_title:Antiviral research
pub_type: 历史文章,杂志文章,评审
doi:10.1016/s0166-3542(02)00197-3
更新日期:2003-01-01 00:00:00
abstract::Itraconazole (ITZ) is a well-known, FDA-approved antifungal drug that is also in clinical trials for its anticancer activity. ITZ exerts its anticancer activity through several disparate targets and pathways. ITZ inhibits angiogenesis by hampering the functioning of the vascular endothelial growth receptor 2 (VEGFR2) ...
journal_title:Antiviral research
pub_type: 杂志文章
doi:10.1016/j.antiviral.2018.05.010
更新日期:2018-08-01 00:00:00
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journal_title:Antiviral research
pub_type: 杂志文章
doi:10.1016/j.antiviral.2015.05.006
更新日期:2015-08-01 00:00:00
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journal_title:Antiviral research
pub_type: 杂志文章
doi:10.1016/j.antiviral.2013.05.013
更新日期:2013-08-01 00:00:00
abstract::HIV-associated neurocognitive disorders (HAND) remain prevalent even with widespread use of combination antiretroviral therapy (ART), suggesting a potential role for co-morbidities in neurologic decline. Indeed, it is well established that ART drugs, particularly HIV protease inhibitors, can induce hyperlipidemia, lip...
journal_title:Antiviral research
pub_type: 杂志文章
doi:10.1016/j.antiviral.2012.04.010
更新日期:2012-07-01 00:00:00
abstract::Drug resistance resulting from reverse transcriptase (RT) mutations is one of the main obstacles to successful hepatitis B virus (HBV) therapy. Indeed, HBV treatment guidelines recommend HBV genotypic resistance testing for patients receiving nucleos(t)ide RT inhibitors (N(t)RTIs) who develop virological failure. N(t)...
journal_title:Antiviral research
pub_type: 杂志文章
doi:10.1016/j.antiviral.2010.09.012
更新日期:2010-12-01 00:00:00
abstract::Enterovirus 71 (EV71) and Coxsackievirus A16 (CVA16) are the two major causative agents of hand, foot and mouth disease (HFMD), for which there are currently no licenced treatments. Here, the acquisition of resistance towards two novel capsid-binding compounds, NLD and ALD, was studied and compared to the analogous co...
journal_title:Antiviral research
pub_type: 杂志文章
doi:10.1016/j.antiviral.2015.10.006
更新日期:2015-12-01 00:00:00
abstract::Avian influenza A(H6N1) virus is one of the most common viruses isolated from migrating birds and domestic poultry in many countries. The first and only known case of human infection by H6N1 virus in the world was reported in Taiwan in 2013. This led to concern that H6N1 virus may cause a threat to public health. In t...
journal_title:Antiviral research
pub_type: 杂志文章
doi:10.1016/j.antiviral.2015.10.019
更新日期:2016-02-01 00:00:00