当前位置： SCI文献检索 > ANTIVIRAL RESEARCH期刊下所有文献 > Transient inhibition of foot-and-mouth disease virus infection of BHK-21 cells by antisense oligonucleotides directed against the second functional initiator AUG.

Transient inhibition of foot-and-mouth disease virus infection of BHK-21 cells by antisense oligonucleotides directed against the second functional initiator AUG.

Abstract：

:The antiviral activity of antisense oligonucleotides corresponding to different regions of foot-and-mouth disease virus (FMDV) genome has been assessed in BHK-21 cells. The locations of the oligonucleotides used were: (i) two regions within the internal ribosome entry site (IRES), involved in the regulation of the translation initiation of the viral polyprotein; (ii) each of the two functional initiator AUGs; (iii) an internal sequence of P2A gene; and (iv) a region at the 3' end non-coding region. Cytoplasmic microinjection of oligodeoxyribonucleotides and oligoribonucleotides complementary to the second AUG resulted in a transient inhibition of viral VP1 expression in infected cells. Significant inhibitions, ranging from 35 to 52%, were obtained at 5 h post-infection using oligonucleotide concentrations of 125 microM and higher. The extent and duration of this inhibition seemed to be mediated by both a rapid transport to the nucleus and the short half-life of the oligonucleotide. This inhibition of FMDV protein synthesis was correlated with a reduction of virus yield of about 50%, as observed after the addition to the cell culture of an oligodeoxyribonucleotide phosphorothioate complementary to the second AUG.

journal_name

Antiviral Res

journal_title

Antiviral research

authors

Gutiérrez A,Rodríguez A,Pintado B,Sobrino F

doi

10.1016/0166-3542(93)90082-t

subject

Has Abstract

pub_date

1993-09-01 00:00:00

pages

1-13

issue

1

eissn

0166-3542

issn

1872-9096

pii

0166-3542(93)90082-T

journal_volume

22

pub_type

杂志文章

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