Abstract：
:Lactoferrin (LF), a member of the transferrin family, is a bi-globular protein secreted in milk, saliva, tears, seminal fluid, endocervix and vaginal secretions. LF is an important player in the defence against pathogenic microorganisms and has also been shown to have activity against several viruses including herpesvirus, adenovirus, rotavirus and poliovirus. The antiviral activity of LF is directed against the early steps of viral infection and the LF antiviral effect against herpesvirus is mediated through LF binding to the herpesvirus receptor heparan sulfate. Human papillomavirus (HPV) causes genital warts and is a prerequisite for cervical cancer. HPV can also use heparan sulfate on the cell surface as a receptor. We studied the inhibition by LF on HPV entry by incubating HaCaT cells and HPV-16 virus-like particles (VLPs) with either human (HLF) or bovine lactoferrin (BLF). LF inhibited internalization of HPV-16 particles using CFDA-SE-labelled VLPs that only fluoresce after internalisation. By using a western blot assay we also found dose-dependent LF inhibition of HPV-16 VLP binding to the HaCaT cell surface. BLF was a more potent inhibitor of HPV entry than human LF. It was also clear that LF acted early in the HPV uptake process.
journal_name
Antiviral Resjournal_title
Antiviral researchauthors
Drobni P,Näslund J,Evander Mdoi
10.1016/j.antiviral.2004.05.005subject
Has Abstractpub_date
2004-10-01 00:00:00pages
63-8issue
1eissn
0166-3542issn
1872-9096pii
S0166-3542(04)00123-8journal_volume
64pub_type
杂志文章abstract：:Epidermal growth factor receptor (EGFr) has been shown to be induced and activated in cells infected with HPV, suggesting that it may play a physiological role in viral replication or in the formation or maintenance of warts. To investigate this possibility, human foreskin tissue was infected with HPV11 and transplant...
journal_title：Antiviral research
pub_type： 杂志文章
doi：10.1016/j.antiviral.2006.12.004
更新日期：2007-04-01 00:00:00
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journal_title：Antiviral research
pub_type： 杂志文章
doi：10.1016/s0166-3542(01)00205-4
更新日期：2002-02-01 00:00:00
abstract：:The ability to rescue an infectious, recombinant, negative-stranded, RNA virus from a complementary DNA (cDNA) clone, has led to new opportunities for measuring viral replication from a viral expressed reporter gene. In this study, the enhanced green fluorescent protein (EGFP) gene was inserted into the human parainfl...
journal_title：Antiviral research
pub_type： 杂志文章
doi：10.1016/j.antiviral.2009.01.001
更新日期：2009-04-01 00:00:00
abstract：:Trimming of the N-glycans attached to the envelope proteins of hepatitis B virus (HBV) is required in different steps of the viral life cycle. Inhibition of the host enzymes alpha-glucosidases, involved in the endoplasmic reticulum (ER)-associated processing of the N-linked glycans, results in misfolding of the HBV en...
journal_title：Antiviral research
pub_type： 杂志文章
doi：10.1016/j.antiviral.2007.04.004
更新日期：2007-10-01 00:00:00
abstract：:Hepatitis B virus (HBV) infection remains a global health problem, causing nearly one million deaths annually. Forkhead box O (FoxO) transcription factors play important roles in modulating diverse physiological processes. Recent studies show that FoxOs are involved in antiviral responses. In present investigation, we...
journal_title：Antiviral research
pub_type： 杂志文章
doi：10.1016/j.antiviral.2019.104568
更新日期：2019-10-01 00:00:00
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journal_title：Antiviral research
pub_type： 杂志文章,评审
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更新日期：2013-06-01 00:00:00
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journal_title：Antiviral research
pub_type： 杂志文章
doi：10.1016/j.antiviral.2020.104867
更新日期：2020-11-01 00:00:00
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journal_title：Antiviral research
pub_type： 杂志文章,评审
doi：10.1016/j.antiviral.2009.02.203
更新日期：2009-06-01 00:00:00
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journal_title：Antiviral research
pub_type： 杂志文章,评审
doi：10.1016/j.antiviral.2013.08.023
更新日期：2013-11-01 00:00:00
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journal_title：Antiviral research
pub_type： 杂志文章
doi：10.1016/j.antiviral.2014.11.003
更新日期：2015-01-01 00:00:00
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journal_title：Antiviral research
pub_type： 杂志文章
doi：10.1016/0166-3542(87)90002-7
更新日期：1987-03-01 00:00:00
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journal_title：Antiviral research
pub_type： 杂志文章
doi：10.1016/0166-3542(95)00038-n
更新日期：1995-09-01 00:00:00
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journal_title：Antiviral research
pub_type： 杂志文章
doi：10.1016/s0166-3542(00)00132-7
更新日期：2001-01-01 00:00:00
abstract：BACKGROUND:Prophylaxis is widely adopted the best choice against Hemorrhagic fever with renal syndrome (HFRS) caused by Hantavirus. However, loss of memory immune response maintenance remains as major shortcoming in current HFRS vaccine. A recombinant DNA vaccine, pVAX-LAMP/Gn was previously proved efficient, requiring...
journal_title：Antiviral research
pub_type： 杂志文章
doi：10.1016/j.antiviral.2016.12.001
更新日期：2017-02-01 00:00:00
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journal_title：Antiviral research
pub_type： 杂志文章
doi：10.1016/j.antiviral.2014.09.002
更新日期：2014-11-01 00:00:00
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journal_title：Antiviral research
pub_type： 杂志文章
doi：10.1016/0166-3542(95)00088-4
更新日期：1995-05-01 00:00:00
abstract：:As part of our studies on the molecular basis for the antiherpes activity of 5'-AdThd (5'-amino-5'-deoxythymidine), a study of the HSV-1 DNA synthesized in infected Vero cells exposed to 5'-AdThd was undertaken. Unlike many other antiviral nucleoside analogs, 5'-AdThd did not inhibit HSV-1 DNA synthesis. Analysis of t...
journal_title：Antiviral research
pub_type： 杂志文章
doi：10.1016/0166-3542(84)90032-9
更新日期：1984-10-01 00:00:00
abstract：:Foot-and-mouth disease virus (FMDV) causes a highly contagious disease of cloven-hoofed animals. We have reported (Cubillos et al., 2008) that a synthetic dendrimeric peptide consisting of four copies of a B-cell epitope [VP1(136-154)] linked through thioether bonds to a T-cell epitope [3A(21-35)] of FMDV [B4T(thi)] e...
journal_title：Antiviral research
pub_type： 杂志文章
doi：10.1016/j.antiviral.2016.03.005
更新日期：2016-05-01 00:00:00
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journal_title：Antiviral research
pub_type： 杂志文章,评审
doi：10.1016/j.antiviral.2015.03.007
更新日期：2015-06-01 00:00:00
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journal_title：Antiviral research
pub_type： 杂志文章,评审
doi：10.1016/j.antiviral.2013.01.007
更新日期：2013-04-01 00:00:00
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journal_title：Antiviral research
pub_type： 杂志文章
doi：10.1016/j.antiviral.2013.05.013
更新日期：2013-08-01 00:00:00
abstract：:The successful development of antiviral drugs is highly dependent on a close interaction and collaboration between the chemist and the biologist (biomedic). This is illustrated by a number of representative examples: S-adenosylhomocysteine (SAH) hydrolase inhibitors which display broad-spectrum antiviral activity, bro...
journal_title：Antiviral research
pub_type： 杂志文章,评审
doi：10.1016/j.antiviral.2005.05.001
更新日期：2005-08-01 00:00:00
abstract：BACKGROUND:By modulating the antiviral immune response via vitamin D receptor, the active form of vitamin D (1,25-dihydroxyvitamin D, calcitriol) could play a central role in protection against respiratory virus infections. This in vitro study tested the hypothesis that respiratory viruses modulate vitamin D receptor e...
journal_title：Antiviral research
pub_type： 杂志文章
doi：10.1016/j.antiviral.2016.11.004
更新日期：2017-01-01 00:00:00
abstract：:SAMHD1 is a triphosphohydrolase that restricts HIV-1 by limiting the intracellular dNTP pool required for reverse transcription. Although SAMHD1 is expressed and active/unphosphorylated in most cell lines, its restriction activity is thought to be relevant only in non-cycling cells. However, an in depth evaluation of ...
journal_title：Antiviral research
pub_type： 杂志文章
doi：10.1016/j.antiviral.2017.03.019
更新日期：2017-06-01 00:00:00
abstract：:Favipiravir (T-705) is a broad spectrum antiviral which has been approved in Japan for the treatment of severe influenza virus infections. We reported earlier that favipiravir inhibits the in vitro replication of CHIKV and protects against disease progression in CHIKV-infected immunodeficient mice. We here explored wh...
journal_title：Antiviral research
pub_type： 杂志文章
doi：10.1016/j.antiviral.2017.09.016
更新日期：2018-01-01 00:00:00
abstract：:T Follicular helper cells (TFH) are considered critical for B cell antibody response, and recent efforts have focused on promoting TFH in order to enhance vaccine efficacy. We studied the frequency and function of TFH in nasopharynx-associated lymphoid tissues (NALT) from children and adults, and its role in anti-infl...
journal_title：Antiviral research
pub_type： 杂志文章
doi：10.1016/j.antiviral.2016.05.021
更新日期：2016-08-01 00:00:00
abstract：:Cell-to-cell contact is usually essential for syncytium formation by HTLV-I-infected cell lines. The present study was undertaken to determine the inhibitory effect of polyanionic compounds, dextran sulfate and heparin, on HTLV-I-induced syncytium formation, as demonstrated by the fusion of HTLV-I-infected cells with ...
journal_title：Antiviral research
pub_type： 杂志文章
doi：10.1016/0166-3542(94)90041-8
更新日期：1994-02-01 00:00:00
abstract：:Statins are 3-hydroxy-3-methylglutaryl coenzyme A (HMG-CoA) reductase inhibitors that are widely used for the treatment of hypercholesterolemia. Besides their cholesterol-lowering effect, statins have been reported to have antiviral activity against a variety of viruses, including hepatitis C virus (HCV). Several stat...
journal_title：Antiviral research
pub_type： 杂志文章
doi：10.1016/j.antiviral.2014.02.020
更新日期：2014-05-01 00:00:00
abstract：:Parvovirus B19 (B19V), a single-stranded DNA virus in the family Parvoviridae, is a human pathogenic virus responsible for a wide range of clinical manifestations. Currently there is no approved antiviral therapy for parvovirus infection. The acyclic nucleoside phosphonate cidofovir (CDV) has been demonstrated to inhi...
journal_title：Antiviral research
pub_type： 杂志文章
doi：10.1016/j.antiviral.2018.12.003
更新日期：2019-02-01 00:00:00
abstract：:Tiazofurin (2-beta-D-ribofuranosylthiazole-4-carboxamide) was examined for its activity against tomato-spotted wilt virus (TSWV) in tomato plants. Solutions containing 50, 100, 200, 400 and 800 mg/l of the drug were sprayed onto the leaves. The results showed that 100 and 200 mg/l were the most efficient concentration...
journal_title：Antiviral research
pub_type： 杂志文章
doi：10.1016/0166-3542(84)90003-2
更新日期：1984-12-01 00:00:00
