Abstract:
:In the scope of the search for new anti-HIV agents interacting with a new target, we developed a high-throughput screening system to detect the interactions between Nef and Hck. Nef is an accessory protein of HIV, which is involved in the pathogenicity of the acquired immunodeficiency syndrome (AIDS). Nef is also a signaling molecule because it binds to many host molecules. It has especially high affinity to Hck, a member of src family tyrosine kinase. Using a mammalian two-hybrid system, the interaction between Nef and the SH3 domain of Hck induced luciferase activity with high sensitivity and a Nef-PXXP peptide inhibited this interaction; and so did the anticancer drug adriamycin. We also developed another assay system by coexpression of full-length Hck and Nef, and found that Hck kinase was activated depending on the dose of Nef plasmid. Using the second system, we found that adriamycin interfered with the Nef-Hck interaction by reducing the amount of the Hck protein. The mammalian two-hybrid system may show utility in screening inhibitors of Nef-Hck interaction.
journal_name
Antiviral Resjournal_title
Antiviral researchauthors
Murakami Y,Fukazawa H,Kobatake T,Yamagoe S,Takebe Y,Tobiume M,Matsuda M,Uehara Ydoi
10.1016/s0166-3542(02)00017-7subject
Has Abstractpub_date
2002-07-01 00:00:00pages
161-8issue
1eissn
0166-3542issn
1872-9096pii
S0166354202000177journal_volume
55pub_type
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pub_type: 临床试验,杂志文章
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pub_type: 杂志文章
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更新日期:2011-01-01 00:00:00
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pub_type: 杂志文章
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