Abstract：
:The acyclic analogue of guanosine acyclovir (ACV) constitutes the first-line drug for the treatment of herpes simplex virus (HSV) infections. ACV activation requires primophosphorylation by virus-encoded HSV thymidine kinase (TK). In 95% of cases, HSV resistance to ACV is associated with mutations located in TK. The aim of this work was to address the question of the potential involvement of novel HSV-1 and HSV-2 TK mutations in reduced susceptibility to ACV using a novel nonradioactive method, based on luminescent quantitation of ADP, for the evaluation of in vitro phosphorylation activity of TK. All recombinant TKs tested exhibited significantly lower ACV phosphorylation activities in comparison with those of reference KOS or gHSV-2 TKs (p<0.015), therefore indicating that amino acid changes Y53D, L170P, R176W, A207P (HSV-1) and S66P, A72S, I101S, M183I (HSV-2) were likely to be involved in HSV resistance to ACV.
journal_name
Antiviral Resjournal_title
Antiviral researchauthors
Burrel S,Bonnafous P,Hubacek P,Agut H,Boutolleau Ddoi
10.1016/j.antiviral.2012.09.016subject
Has Abstractpub_date
2012-12-01 00:00:00pages
386-90issue
3eissn
0166-3542issn
1872-9096pii
S0166-3542(12)00223-9journal_volume
96pub_type
杂志文章abstract：:Maraviroc, the only CCR5 antagonist HIV inhibitor currently approved, has potent antiviral activity in treatment-experienced individuals infected with CCR5-using HIV-1 (R5 HIV-1). However, recent data from the MOTIVATE trials indicate that R5 HIV-1 can develop resistance to Maraviroc, underscoring the need for additio...
journal_title：Antiviral research
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更新日期：2009-07-01 00:00:00
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pub_type： 杂志文章
doi：10.1016/0166-3542(89)90036-3
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journal_title：Antiviral research
pub_type： 杂志文章
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更新日期：2011-03-01 00:00:00
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journal_title：Antiviral research
pub_type： 杂志文章
doi：10.1016/s0166-3542(01)00150-4
更新日期：2001-09-01 00:00:00
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journal_title：Antiviral research
pub_type： 杂志文章
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更新日期：2006-02-01 00:00:00
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journal_title：Antiviral research
pub_type： 杂志文章
doi：10.1016/0166-3542(94)00064-f
更新日期：1995-01-01 00:00:00
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journal_title：Antiviral research
pub_type： 杂志文章
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更新日期：2020-01-01 00:00:00
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journal_title：Antiviral research
pub_type： 杂志文章
doi：10.1016/j.antiviral.2015.03.009
更新日期：2015-06-01 00:00:00
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journal_title：Antiviral research
pub_type： 杂志文章
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更新日期：2002-07-01 00:00:00
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journal_title：Antiviral research
pub_type： 杂志文章
doi：10.1016/j.antiviral.2020.104996
更新日期：2021-01-01 00:00:00
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journal_title：Antiviral research
pub_type： 杂志文章
doi：10.1016/j.antiviral.2006.12.005
更新日期：2007-04-01 00:00:00
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journal_title：Antiviral research
pub_type： 杂志文章
doi：10.1016/j.antiviral.2012.02.001
更新日期：2012-04-01 00:00:00
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journal_title：Antiviral research
pub_type： 杂志文章
doi：10.1016/j.antiviral.2006.02.003
更新日期：2006-07-01 00:00:00
abstract：:Epidermal growth factor receptor (EGFr) has been shown to be induced and activated in cells infected with HPV, suggesting that it may play a physiological role in viral replication or in the formation or maintenance of warts. To investigate this possibility, human foreskin tissue was infected with HPV11 and transplant...
journal_title：Antiviral research
pub_type： 杂志文章
doi：10.1016/j.antiviral.2006.12.004
更新日期：2007-04-01 00:00:00
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journal_title：Antiviral research
pub_type： 杂志文章
doi：10.1016/s0166-3542(02)00017-7
更新日期：2002-07-01 00:00:00
abstract：:T Follicular helper cells (TFH) are considered critical for B cell antibody response, and recent efforts have focused on promoting TFH in order to enhance vaccine efficacy. We studied the frequency and function of TFH in nasopharynx-associated lymphoid tissues (NALT) from children and adults, and its role in anti-infl...
journal_title：Antiviral research
pub_type： 杂志文章
doi：10.1016/j.antiviral.2016.05.021
更新日期：2016-08-01 00:00:00
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journal_title：Antiviral research
pub_type： 杂志文章,评审
doi：10.1016/s0166-3542(03)00008-1
更新日期：2003-04-01 00:00:00
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journal_title：Antiviral research
pub_type： 杂志文章
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更新日期：2020-05-01 00:00:00
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journal_title：Antiviral research
pub_type： 杂志文章
doi：10.1016/0166-3542(94)90018-3
更新日期：1994-04-01 00:00:00
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journal_title：Antiviral research
pub_type： 杂志文章
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更新日期：2018-09-01 00:00:00
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journal_title：Antiviral research
pub_type： 临床试验,杂志文章,随机对照试验
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更新日期：1995-08-01 00:00:00
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journal_title：Antiviral research
pub_type： 杂志文章
doi：10.1016/j.antiviral.2014.02.015
更新日期：2014-05-01 00:00:00
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journal_title：Antiviral research
pub_type： 杂志文章
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更新日期：2012-09-01 00:00:00
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journal_title：Antiviral research
pub_type： 杂志文章
doi：10.1016/j.antiviral.2016.01.010
更新日期：2016-05-01 00:00:00
abstract：:Carbovir (CBV) is a guanine nucleoside analog with potent in vitro anti-HIV activity. A prodrug of CBV is currently being evaluated in clinical trials as a potential agent for the treatment of AIDS. The ability of CBV to inhibit mitochondrial DNA synthesis in intact CEM cells was evaluated in the present study, becaus...
journal_title：Antiviral research
pub_type： 杂志文章
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更新日期：1997-05-01 00:00:00
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journal_title：Antiviral research
pub_type： 杂志文章
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更新日期：2021-01-01 00:00:00
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journal_title：Antiviral research
pub_type： 杂志文章
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更新日期：2019-08-01 00:00:00
abstract：:Although canine rabies has been eliminated from industrialized countries, infected dogs remain the primary source of human and livestock exposures in Asia, Africa and much of South America. Human deaths are the most important direct economic impact of canine rabies, followed by livestock losses and the cost of PEP, wh...
journal_title：Antiviral research
pub_type： 杂志文章,评审
doi：10.1016/j.antiviral.2013.03.004
更新日期：2013-05-01 00:00:00
abstract：:Nucleoside analogues have been the most successful antiviral compounds. Likewise, they are the most intriguing antiviral compounds, because of their structural relationship to natural nucleosides. This is also the reason why the design process of a potential selective antiviral nucleoside is so difficult. Too many nat...
journal_title：Antiviral research
pub_type： 杂志文章,评审
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更新日期：2006-09-01 00:00:00
abstract：:We investigated the development of anti-pre-S(2) antibodies by enzyme immunoassay and by Western blot analysis in a vaccination study in haemodialysis patients; both the Pasteur plasma vaccine retaining the pre-S(2) epitopes and the Merck, Sharp and Dohme (MSD) plasma vaccine containing only HBsAg were used. By enzyme...
journal_title：Antiviral research
pub_type： 杂志文章
doi：10.1016/0166-3542(89)90071-5
更新日期：1989-09-01 00:00:00
