Abstract:
:Oligodeoxynucleotides (ODNs) composed entirely of deoxyguanosine and thymidine, but not specifically designed to act as antisense agents, were able to significantly inhibit herpes simplex virus growth in acute infection assay systems. The guanosine/thymidine (GT) ODNs which demonstrated this antiviral activity contained either natural phosphodiester (PO) or phosphorothioate (PS) modified internucleoside linkages. In all experiments, the antiviral activity of the ODNs was enhanced when the backbone was modified to contain the PS linkages. When present during the time of virus addition, the ODNs were able to block the adsorption of virus to Vero cells. In this assay the PS-containing ODNs had ID50 values of approximately 0.020 microM for HSV-2 and of 0.3 microM for HSV-1. When these same PS-containing ODNs were used against HSV-2 in single-cycle viral yield assays, designed to minimize the effects due to external blockage of virus, the ID50 values rose to 0.2 microM. Analysis of viral DNA obtained 14 h post-HSV-2 infections in the single-cycle assay, revealed a decrease in replicated viral DNA in cells treated with PS-ODNs. Analysis of viral mRNA obtained 4 h post-HSV-2 infection revealed, in cells treated with the PS-ODNs, a decrease in measurable HSV-2 alpha- and beta-mRNAs. Although the mechanism of action of the antiviral activity (beyond adsorption blocking) is not fully understood, the toxicity of these compounds was low, giving high therapeutic indices for the GT-rich PS-ODNs. The good therapeutic index of GT-ODNs make this a class of compounds which warrant investigation as therapeutic agents to be used against herpes viruses.
journal_name
Antiviral Resjournal_title
Antiviral researchauthors
Fennewald SM,Mustain S,Ojwang J,Rando RFdoi
10.1016/0166-3542(94)00064-fsubject
Has Abstractpub_date
1995-01-01 00:00:00pages
37-54issue
1eissn
0166-3542issn
1872-9096pii
016635429400064Fjournal_volume
26pub_type
杂志文章abstract::JNJ63623872 (formerly known as VX-787) is an inhibitor of influenza A virus polymerases through interaction with the viral PB2 subunit. This interaction blocks the cap-snatching activity of the virus that is essential for virus replication. Previously published work has documented antiviral activity of JNJ63623872 in ...
journal_title:Antiviral research
pub_type: 杂志文章
doi:10.1016/j.antiviral.2016.10.009
更新日期:2016-12-01 00:00:00
abstract::Enterovirus-71 (EV71) is one of the major causative reagents for hand-foot-and-mouth disease. In particular, EV71 causes severe central nervous system infections and leads to numerous dead cases. Although several inactivated whole-virus vaccines have entered in clinical trials, no antiviral agent has been provided for...
journal_title:Antiviral research
pub_type: 杂志文章
doi:10.1016/j.antiviral.2013.12.008
更新日期:2014-03-01 00:00:00
abstract::A severe inflammatory immune response with hypercytokinemia occurs in patients hospitalized with severe influenza, such as avian influenza A(H5N1), A(H7N9), and seasonal A(H1N1)pdm09 virus infections. The role of immunomodulatory therapy is unclear as there have been limited published data based on randomized controll...
journal_title:Antiviral research
pub_type: 杂志文章,评审
doi:10.1016/j.antiviral.2018.01.002
更新日期:2018-02-01 00:00:00
abstract::Preventive intraperitoneal trehalose dimycolate (TDM) treatment of mice, inoculated with encephalomyocarditis (EMC) virus by the same route, caused restriction of virus growth in the peritoneum, which was correlated to IFN production in peritoneal fluids prior to infection. Peritoneal macrophages from TDM-treated mice...
journal_title:Antiviral research
pub_type: 杂志文章
doi:10.1016/0166-3542(95)00047-p
更新日期:1995-10-01 00:00:00
abstract::DAS181 is an inhaled bacterial sialidase which functions by removing sialic acid (Sia) from the surface of epithelial cells, preventing attachment and subsequent infection by respiratory viruses that utilize Sia as a receptor. DAS181 is typical of bacterial sialidases in cleaving Sia α2-3 and Sia α2-6 linkages, and it...
journal_title:Antiviral research
pub_type: 杂志文章,评审
doi:10.1016/j.antiviral.2013.04.012
更新日期:2013-06-01 00:00:00
abstract::Laninamivir, a potent neuraminidase (NA) inhibitor, is an active metabolite of laninamivir octanoate (code name: CS-8958) which is a long acting NA inhibitor and is commercially available under the brand name Inavir in Japan to complete the treatment of influenza by a single inhalation. It is supposed that the long ac...
journal_title:Antiviral research
pub_type: 杂志文章
doi:10.1016/j.antiviral.2013.08.004
更新日期:2013-10-01 00:00:00
abstract::The biological response modifier 7-thia-8-oxoguanosine was evaluated in mice against the hepatotropic Adames strain of Punta Toro virus. When administered intraperitoneally in divided doses, significant protection from death was conferred at doses of 50 and 100 mg/kg/day given 24 and 17 h pre-virus inoculation, 25-100...
journal_title:Antiviral research
pub_type: 杂志文章
doi:10.1016/0166-3542(91)90069-4
更新日期:1991-03-01 00:00:00
abstract::Tiazofurin (2-beta-D-ribofuranosylthiazole-4-carboxamide) was examined for its activity against tomato-spotted wilt virus (TSWV) in tomato plants. Solutions containing 50, 100, 200, 400 and 800 mg/l of the drug were sprayed onto the leaves. The results showed that 100 and 200 mg/l were the most efficient concentration...
journal_title:Antiviral research
pub_type: 杂志文章
doi:10.1016/0166-3542(84)90003-2
更新日期:1984-12-01 00:00:00
abstract::The heterocycle, 4,6-dibenzamidopyrazolo[3,4-d]pyrimidine (DBAPP), inhibited cytopathology induced by human, mouse, and vervet monkey cytomegaloviruses (CMV) in vitro at 0.2 to 0.5 microM, but did not inhibit cell replication at less than or equal to 30 microM. Herpes simplex viruses were unaffected by the inhibitor. ...
journal_title:Antiviral research
pub_type: 杂志文章
doi:10.1016/0166-3542(90)90040-e
更新日期:1990-01-01 00:00:00
abstract:OBJECTIVE:We sought to investigate the differences in monocyte immune responses to the dengue virus (DENV) in those who previously had either severe disease (past SD) or non-severe dengue (past NSD) following a secondary dengue infection. METHOD:Monocytes from healthy individuals who had either past SD (n = 6) or past...
journal_title:Antiviral research
pub_type: 杂志文章
doi:10.1016/j.antiviral.2019.104554
更新日期:2019-09-01 00:00:00
abstract::HIV-1 integrase (IN) is one of three essential enzymes (along with reverse transcriptase and protease) encoded by the viral pol gene. IN mediates two critical reactions during viral replication; firstly 3'-end processing (3'EP) of the double-stranded viral DNA ends and then strand transfer (STF) which joins the viral ...
journal_title:Antiviral research
pub_type: 历史文章,杂志文章,评审
doi:10.1016/j.antiviral.2009.11.004
更新日期:2010-01-01 00:00:00
abstract::Drug resistance testing has proven its use to guide treatment decisions in HIV-1 infected patients. Genotyping is the preferred technique for clinical drug resistance testing. Many factors complicate the interpretation of mutations towards therapy response, such that an interpretation system is necessary to help the c...
journal_title:Antiviral research
pub_type: 杂志文章,评审
doi:10.1016/j.antiviral.2006.05.003
更新日期:2006-09-01 00:00:00
abstract::Dengue virus (DENV) infection is one of the most important infectious diseases in tropical and subtropical regions around the world. Previously, we performed an initial phenotypic screening of 7000 compounds using DENV type 2 (DENV2)-infected BHK-21 cells to identify small molecules which could inhibit virus replicati...
journal_title:Antiviral research
pub_type: 杂志文章
doi:10.1016/j.antiviral.2020.104969
更新日期:2020-12-01 00:00:00
abstract::JLS-V9R cells, a Balb/c mouse bone marrow cell line chronically infected with Rauscher leukemia virus, were treated with mouse interferon and inoculated with several different lytic viruses. Relatively low interferon concentrations protected the cells against Sindbis virus, vesicular stomatitis virus and MM virus. In ...
journal_title:Antiviral research
pub_type: 杂志文章
doi:10.1016/0166-3542(84)90006-8
更新日期:1984-12-01 00:00:00
abstract::This study aimed to investigate anti-HBV effect and major active compounds of Su-duxing, a medicine extracted from Chinese herbs. HBV-replicating cell lines HepG2.2.15 (wild-type) and HepG2.A64 (entecavir-resistant) were used for in vitro test. C57BL/6 mice infected by adeno-associated virus carrying 1.3 mer wild-type...
journal_title:Antiviral research
pub_type: 杂志文章
doi:10.1016/j.antiviral.2018.04.017
更新日期:2018-07-01 00:00:00
abstract::The successful development of antiviral drugs is highly dependent on a close interaction and collaboration between the chemist and the biologist (biomedic). This is illustrated by a number of representative examples: S-adenosylhomocysteine (SAH) hydrolase inhibitors which display broad-spectrum antiviral activity, bro...
journal_title:Antiviral research
pub_type: 杂志文章,评审
doi:10.1016/j.antiviral.2005.05.001
更新日期:2005-08-01 00:00:00
abstract::Immune evasion by the lethal henipaviruses, Hendra (HeV) and Nipah virus, is mediated by its interferon (IFN) antagonist P gene products, phosphoprotein (P), and the related V and W proteins, which can target the signal transducer and activator of transcription 1 (STAT1) and STAT2 proteins to inhibit IFN/STAT signalin...
journal_title:Antiviral research
pub_type: 杂志文章
doi:10.1016/j.antiviral.2015.09.017
更新日期:2015-12-01 00:00:00
abstract::In cultured mammalian cells (Vero), different antiviral agents change to differing degrees the ability of HSV2 to down-regulate gap junctions, each agent having a specific effect. Measured by intracellular electrodes, control cell populations showed 49-51% coupling, uninfected populations treated with acyclovir or SDS...
journal_title:Antiviral research
pub_type: 杂志文章
doi:10.1016/s0166-3542(02)00106-7
更新日期:2002-11-01 00:00:00
abstract::A recombinant baculovirus (vAc-gC1) was constructed that expresses the glycoprotein C (gC) gene of herpes simplex virus type 1 (HSV-1). When Sf9 cells were infected with this recombinant, a protein that was smaller in size than authentic HSV-1 gC was detected by Western blotting using anti-gC polyclonal antibody. The ...
journal_title:Antiviral research
pub_type: 杂志文章
doi:10.1016/0166-3542(92)90062-a
更新日期:1992-06-01 00:00:00
abstract::Brivudin [(E)-5-(2-bromovinyl)-2'-deoxyuridine] is a nucleoside analogue with a high and selective antiviral activity against varicella-zoster virus (VZV) and herpes simplex virus type 1 (HSV-1). The double-blind, randomized study presented here compared efficacy and safety of oral brivudin 1 x 125 mg and acyclovir 5 ...
journal_title:Antiviral research
pub_type: 临床试验,杂志文章,多中心研究,随机对照试验
doi:10.1016/s0166-3542(03)00065-2
更新日期:2003-06-01 00:00:00
abstract::We studied the effects of two antiviral agents, human interferon-alpha (IFN-alpha) and ribavirin, on subacute sclerosing panencephalitis (SSPE) virus infections in hamsters. By intracranial administration, IFN-alpha alone improved the survival of infected hamsters by 20% at a dose of 6 x 10(4) IU/kg every other day fo...
journal_title:Antiviral research
pub_type: 杂志文章
doi:10.1016/s0166-3542(97)00055-7
更新日期:1998-01-01 00:00:00
abstract::Almost all cases of human rabies result from dog bites, making the elimination of canine rabies a global priority. During recent decades, many countries in the Western Hemisphere have carried out large-scale dog vaccination campaigns, controlled their free-ranging dog populations and enforced legislation for responsib...
journal_title:Antiviral research
pub_type: 历史文章,杂志文章,评审
doi:10.1016/j.antiviral.2017.03.023
更新日期:2017-07-01 00:00:00
abstract::Despite an urgent medical need, a broadly effective anti-viral therapy for the treatment of infections with hepatitis C viruses (HCVs) has yet to be developed. One of the approaches to anti-HCV drug discovery is the design and development of specific small molecule drugs to inhibit the proteolytic processing of the HC...
journal_title:Antiviral research
pub_type: 杂志文章,评审
doi:
更新日期:1999-02-01 00:00:00
abstract::Stilbenoids including resveratrol contain the basic structural unit of 1,2-diphenylethylene. Naturally occurring stilbenoids have broad structural features due to oligomerization and modifications and some have demonstrated potent biological activities. In an effort to identify bioactive stilbenoids, we screened a gro...
journal_title:Antiviral research
pub_type: 杂志文章
doi:10.1016/j.antiviral.2012.05.001
更新日期:2012-07-01 00:00:00
abstract::This review describes the procedures for the use of fluorochrome labeled monoclonal antibodies and flow cytometry for the detection and quantification of virus infected cells. The application of this technology for (1) identifying virus infected cells in clinical specimens obtained from human cytomegalovirus (HCMV) an...
journal_title:Antiviral research
pub_type: 杂志文章,评审
doi:10.1016/s0166-3542(99)00039-x
更新日期:1999-08-01 00:00:00
abstract::Filoviruses, which include Ebola virus (EBOV) and Marburg virus, are negative-sense RNA viruses associated with sporadic outbreaks of severe viral hemorrhagic fever characterized by uncontrolled virus replication. The extreme virulence and emerging nature of these zoonotic pathogens make them a significant threat to h...
journal_title:Antiviral research
pub_type: 杂志文章,评审
doi:10.1016/j.antiviral.2018.12.006
更新日期:2019-02-01 00:00:00
abstract:BACKGROUND:Prophylaxis is widely adopted the best choice against Hemorrhagic fever with renal syndrome (HFRS) caused by Hantavirus. However, loss of memory immune response maintenance remains as major shortcoming in current HFRS vaccine. A recombinant DNA vaccine, pVAX-LAMP/Gn was previously proved efficient, requiring...
journal_title:Antiviral research
pub_type: 杂志文章
doi:10.1016/j.antiviral.2016.12.001
更新日期:2017-02-01 00:00:00
abstract::Zika virus (ZIKV) is a mosquito-borne flavivirus that mostly causes asymptomatic infections or mild disease characterized by low-grade fever, rash, conjunctivitis, and malaise. However, the recent massive ZIKV epidemics in the Americas have also linked ZIKV infection to fetal malformations like microcephaly and Guilla...
journal_title:Antiviral research
pub_type: 杂志文章
doi:10.1016/j.antiviral.2017.04.016
更新日期:2017-07-01 00:00:00
abstract::Citrusinine-I, a new acridone alkaloid isolated from the root bark of the citrus plant (Rutaceae), exhibited potent activity against herpes simplex virus (HSV) type 1 and type 2 at low concentrations relative to their cytotoxicity; 50% effective concentrations (ED50) of citrusinine-I were 0.56 micrograms/ml and 0.74 m...
journal_title:Antiviral research
pub_type: 杂志文章
doi:10.1016/0166-3542(89)90065-x
更新日期:1989-08-01 00:00:00
abstract::Enterovirus 71 (EV71) and Coxsackievirus A16 (CVA16) are the two major causative agents of hand, foot and mouth disease (HFMD), for which there are currently no licenced treatments. Here, the acquisition of resistance towards two novel capsid-binding compounds, NLD and ALD, was studied and compared to the analogous co...
journal_title:Antiviral research
pub_type: 杂志文章
doi:10.1016/j.antiviral.2015.10.006
更新日期:2015-12-01 00:00:00