Inhibition of herpes simplex virus in culture by oligonucleotides composed entirely of deoxyguanosine and thymidine.

Abstract:

:Oligodeoxynucleotides (ODNs) composed entirely of deoxyguanosine and thymidine, but not specifically designed to act as antisense agents, were able to significantly inhibit herpes simplex virus growth in acute infection assay systems. The guanosine/thymidine (GT) ODNs which demonstrated this antiviral activity contained either natural phosphodiester (PO) or phosphorothioate (PS) modified internucleoside linkages. In all experiments, the antiviral activity of the ODNs was enhanced when the backbone was modified to contain the PS linkages. When present during the time of virus addition, the ODNs were able to block the adsorption of virus to Vero cells. In this assay the PS-containing ODNs had ID50 values of approximately 0.020 microM for HSV-2 and of 0.3 microM for HSV-1. When these same PS-containing ODNs were used against HSV-2 in single-cycle viral yield assays, designed to minimize the effects due to external blockage of virus, the ID50 values rose to 0.2 microM. Analysis of viral DNA obtained 14 h post-HSV-2 infections in the single-cycle assay, revealed a decrease in replicated viral DNA in cells treated with PS-ODNs. Analysis of viral mRNA obtained 4 h post-HSV-2 infection revealed, in cells treated with the PS-ODNs, a decrease in measurable HSV-2 alpha- and beta-mRNAs. Although the mechanism of action of the antiviral activity (beyond adsorption blocking) is not fully understood, the toxicity of these compounds was low, giving high therapeutic indices for the GT-rich PS-ODNs. The good therapeutic index of GT-ODNs make this a class of compounds which warrant investigation as therapeutic agents to be used against herpes viruses.

journal_name

Antiviral Res

journal_title

Antiviral research

authors

Fennewald SM,Mustain S,Ojwang J,Rando RF

doi

10.1016/0166-3542(94)00064-f

subject

Has Abstract

pub_date

1995-01-01 00:00:00

pages

37-54

issue

1

eissn

0166-3542

issn

1872-9096

pii

016635429400064F

journal_volume

26

pub_type

杂志文章
  • Activities of JNJ63623872 and oseltamivir against influenza A H1N1pdm and H3N2 virus infections in mice.

    abstract::JNJ63623872 (formerly known as VX-787) is an inhibitor of influenza A virus polymerases through interaction with the viral PB2 subunit. This interaction blocks the cap-snatching activity of the virus that is essential for virus replication. Previously published work has documented antiviral activity of JNJ63623872 in ...

    journal_title:Antiviral research

    pub_type: 杂志文章

    doi:10.1016/j.antiviral.2016.10.009

    authors: Smee DF,Barnard DL,Jones SM

    更新日期:2016-12-01 00:00:00

  • Suramin inhibits EV71 infection.

    abstract::Enterovirus-71 (EV71) is one of the major causative reagents for hand-foot-and-mouth disease. In particular, EV71 causes severe central nervous system infections and leads to numerous dead cases. Although several inactivated whole-virus vaccines have entered in clinical trials, no antiviral agent has been provided for...

    journal_title:Antiviral research

    pub_type: 杂志文章

    doi:10.1016/j.antiviral.2013.12.008

    authors: Wang Y,Qing J,Sun Y,Rao Z

    更新日期:2014-03-01 00:00:00

  • The role of adjuvant immunomodulatory agents for treatment of severe influenza.

    abstract::A severe inflammatory immune response with hypercytokinemia occurs in patients hospitalized with severe influenza, such as avian influenza A(H5N1), A(H7N9), and seasonal A(H1N1)pdm09 virus infections. The role of immunomodulatory therapy is unclear as there have been limited published data based on randomized controll...

    journal_title:Antiviral research

    pub_type: 杂志文章,评审

    doi:10.1016/j.antiviral.2018.01.002

    authors: Hui DS,Lee N,Chan PK,Beigel JH

    更新日期:2018-02-01 00:00:00

  • Role of trehalose dimycolate-induced interferon-alpha/beta in the restriction of encephalomyocarditis virus growth in vivo and in peritoneal macrophage cultures.

    abstract::Preventive intraperitoneal trehalose dimycolate (TDM) treatment of mice, inoculated with encephalomyocarditis (EMC) virus by the same route, caused restriction of virus growth in the peritoneum, which was correlated to IFN production in peritoneal fluids prior to infection. Peritoneal macrophages from TDM-treated mice...

    journal_title:Antiviral research

    pub_type: 杂志文章

    doi:10.1016/0166-3542(95)00047-p

    authors: Guillemard E,Geniteau-Legendre M,Kergot R,Lemaire G,Petit JF,Labarre C,Quero AM

    更新日期:1995-10-01 00:00:00

  • The use of sialidase therapy for respiratory viral infections.

    abstract::DAS181 is an inhaled bacterial sialidase which functions by removing sialic acid (Sia) from the surface of epithelial cells, preventing attachment and subsequent infection by respiratory viruses that utilize Sia as a receptor. DAS181 is typical of bacterial sialidases in cleaving Sia α2-3 and Sia α2-6 linkages, and it...

    journal_title:Antiviral research

    pub_type: 杂志文章,评审

    doi:10.1016/j.antiviral.2013.04.012

    authors: Nicholls JM,Moss RB,Haslam SM

    更新日期:2013-06-01 00:00:00

  • Efficacy of a single intravenous administration of laninamivir (an active metabolite of laninamivir octanoate) in an influenza virus infection mouse model.

    abstract::Laninamivir, a potent neuraminidase (NA) inhibitor, is an active metabolite of laninamivir octanoate (code name: CS-8958) which is a long acting NA inhibitor and is commercially available under the brand name Inavir in Japan to complete the treatment of influenza by a single inhalation. It is supposed that the long ac...

    journal_title:Antiviral research

    pub_type: 杂志文章

    doi:10.1016/j.antiviral.2013.08.004

    authors: Kakuta M,Kubo S,Tanaka M,Tobiume S,Tomozawa T,Yamashita M

    更新日期:2013-10-01 00:00:00

  • Prophylactic and therapeutic activities of 7-thia-8-oxoguanosine against Punta Toro virus infections in mice.

    abstract::The biological response modifier 7-thia-8-oxoguanosine was evaluated in mice against the hepatotropic Adames strain of Punta Toro virus. When administered intraperitoneally in divided doses, significant protection from death was conferred at doses of 50 and 100 mg/kg/day given 24 and 17 h pre-virus inoculation, 25-100...

    journal_title:Antiviral research

    pub_type: 杂志文章

    doi:10.1016/0166-3542(91)90069-4

    authors: Smee DF,Huffman JH,Gessaman AC,Huggins JW,Sidwell RW

    更新日期:1991-03-01 00:00:00

  • Effect of tiazofurin on tomato plants infected with tomato spotted wilt virus.

    abstract::Tiazofurin (2-beta-D-ribofuranosylthiazole-4-carboxamide) was examined for its activity against tomato-spotted wilt virus (TSWV) in tomato plants. Solutions containing 50, 100, 200, 400 and 800 mg/l of the drug were sprayed onto the leaves. The results showed that 100 and 200 mg/l were the most efficient concentration...

    journal_title:Antiviral research

    pub_type: 杂志文章

    doi:10.1016/0166-3542(84)90003-2

    authors: Caner J,Amélia M,Alexandre V,Vicente M

    更新日期:1984-12-01 00:00:00

  • 4,6-dibenzamidopyrazolo[3,4-d]pyrimidine is a highly selective inhibitor of cytomegalovirus adsorption to cells.

    abstract::The heterocycle, 4,6-dibenzamidopyrazolo[3,4-d]pyrimidine (DBAPP), inhibited cytopathology induced by human, mouse, and vervet monkey cytomegaloviruses (CMV) in vitro at 0.2 to 0.5 microM, but did not inhibit cell replication at less than or equal to 30 microM. Herpes simplex viruses were unaffected by the inhibitor. ...

    journal_title:Antiviral research

    pub_type: 杂志文章

    doi:10.1016/0166-3542(90)90040-e

    authors: Smee DF,Bartlett ML,Alaghamandan HA,Jones MM,Revankar GR,Robins RK

    更新日期:1990-01-01 00:00:00

  • Altered monocyte response to the dengue virus in those with varying severity of past dengue infection.

    abstract:OBJECTIVE:We sought to investigate the differences in monocyte immune responses to the dengue virus (DENV) in those who previously had either severe disease (past SD) or non-severe dengue (past NSD) following a secondary dengue infection. METHOD:Monocytes from healthy individuals who had either past SD (n = 6) or past...

    journal_title:Antiviral research

    pub_type: 杂志文章

    doi:10.1016/j.antiviral.2019.104554

    authors: Kamaladasa A,Gomes L,Wijesinghe A,Jeewandara C,Toh YX,Jayathilaka D,Ogg GS,Fink K,Malavige GN

    更新日期:2019-09-01 00:00:00

  • Strand transfer inhibitors of HIV-1 integrase: bringing IN a new era of antiretroviral therapy.

    abstract::HIV-1 integrase (IN) is one of three essential enzymes (along with reverse transcriptase and protease) encoded by the viral pol gene. IN mediates two critical reactions during viral replication; firstly 3'-end processing (3'EP) of the double-stranded viral DNA ends and then strand transfer (STF) which joins the viral ...

    journal_title:Antiviral research

    pub_type: 历史文章,杂志文章,评审

    doi:10.1016/j.antiviral.2009.11.004

    authors: McColl DJ,Chen X

    更新日期:2010-01-01 00:00:00

  • Algorithms for the interpretation of HIV-1 genotypic drug resistance information.

    abstract::Drug resistance testing has proven its use to guide treatment decisions in HIV-1 infected patients. Genotyping is the preferred technique for clinical drug resistance testing. Many factors complicate the interpretation of mutations towards therapy response, such that an interpretation system is necessary to help the c...

    journal_title:Antiviral research

    pub_type: 杂志文章,评审

    doi:10.1016/j.antiviral.2006.05.003

    authors: Vercauteren J,Vandamme AM

    更新日期:2006-09-01 00:00:00

  • Identification of quinolone derivatives as effective anti-Dengue virus agents.

    abstract::Dengue virus (DENV) infection is one of the most important infectious diseases in tropical and subtropical regions around the world. Previously, we performed an initial phenotypic screening of 7000 compounds using DENV type 2 (DENV2)-infected BHK-21 cells to identify small molecules which could inhibit virus replicati...

    journal_title:Antiviral research

    pub_type: 杂志文章

    doi:10.1016/j.antiviral.2020.104969

    authors: Nobori H,Uemura K,Toba S,Sanaki T,Shishido T,Hall WW,Orba Y,Sawa H,Sato A

    更新日期:2020-12-01 00:00:00

  • Disparate response of encephalomyocarditis virus and MM virus to interferon in JLS-V9R cells.

    abstract::JLS-V9R cells, a Balb/c mouse bone marrow cell line chronically infected with Rauscher leukemia virus, were treated with mouse interferon and inoculated with several different lytic viruses. Relatively low interferon concentrations protected the cells against Sindbis virus, vesicular stomatitis virus and MM virus. In ...

    journal_title:Antiviral research

    pub_type: 杂志文章

    doi:10.1016/0166-3542(84)90006-8

    authors: Czarniecki CW,Allen PT

    更新日期:1984-12-01 00:00:00

  • Chinese herbal extract Su-duxing had potent inhibitory effects on both wild-type and entecavir-resistant hepatitis B virus (HBV) in vitro and effectively suppressed HBV replication in mouse model.

    abstract::This study aimed to investigate anti-HBV effect and major active compounds of Su-duxing, a medicine extracted from Chinese herbs. HBV-replicating cell lines HepG2.2.15 (wild-type) and HepG2.A64 (entecavir-resistant) were used for in vitro test. C57BL/6 mice infected by adeno-associated virus carrying 1.3 mer wild-type...

    journal_title:Antiviral research

    pub_type: 杂志文章

    doi:10.1016/j.antiviral.2018.04.017

    authors: Liu Y,Yao W,Si L,Hou J,Wang J,Xu Z,Li W,Chen J,Li R,Li P,Bo L,Xiao X,Lan J,Xu D

    更新日期:2018-07-01 00:00:00

  • Antiviral drug discovery and development: where chemistry meets with biomedicine.

    abstract::The successful development of antiviral drugs is highly dependent on a close interaction and collaboration between the chemist and the biologist (biomedic). This is illustrated by a number of representative examples: S-adenosylhomocysteine (SAH) hydrolase inhibitors which display broad-spectrum antiviral activity, bro...

    journal_title:Antiviral research

    pub_type: 杂志文章,评审

    doi:10.1016/j.antiviral.2005.05.001

    authors: De Clercq E

    更新日期:2005-08-01 00:00:00

  • The non-pathogenic Henipavirus Cedar paramyxovirus phosphoprotein has a compromised ability to target STAT1 and STAT2.

    abstract::Immune evasion by the lethal henipaviruses, Hendra (HeV) and Nipah virus, is mediated by its interferon (IFN) antagonist P gene products, phosphoprotein (P), and the related V and W proteins, which can target the signal transducer and activator of transcription 1 (STAT1) and STAT2 proteins to inhibit IFN/STAT signalin...

    journal_title:Antiviral research

    pub_type: 杂志文章

    doi:10.1016/j.antiviral.2015.09.017

    authors: Lieu KG,Marsh GA,Wang LF,Netter HJ

    更新日期:2015-12-01 00:00:00

  • Antiviral agents alter ability of HSV-2 to disrupt gap junctional intercellular communication between mammalian cells in vitro.

    abstract::In cultured mammalian cells (Vero), different antiviral agents change to differing degrees the ability of HSV2 to down-regulate gap junctions, each agent having a specific effect. Measured by intracellular electrodes, control cell populations showed 49-51% coupling, uninfected populations treated with acyclovir or SDS...

    journal_title:Antiviral research

    pub_type: 杂志文章

    doi:10.1016/s0166-3542(02)00106-7

    authors: Musée J,Mbuy GN,Woodruff RI

    更新日期:2002-11-01 00:00:00

  • Baculovirus expressed herpes simplex virus type 1 glycoprotein C protects mice from lethal HSV-1 infection.

    abstract::A recombinant baculovirus (vAc-gC1) was constructed that expresses the glycoprotein C (gC) gene of herpes simplex virus type 1 (HSV-1). When Sf9 cells were infected with this recombinant, a protein that was smaller in size than authentic HSV-1 gC was detected by Western blotting using anti-gC polyclonal antibody. The ...

    journal_title:Antiviral research

    pub_type: 杂志文章

    doi:10.1016/0166-3542(92)90062-a

    authors: Ghiasi H,Kaiwar R,Nesburn AB,Wechsler SL

    更新日期:1992-06-01 00:00:00

  • Oral brivudin in comparison with acyclovir for improved therapy of herpes zoster in immunocompetent patients: results of a randomized, double-blind, multicentered study.

    abstract::Brivudin [(E)-5-(2-bromovinyl)-2'-deoxyuridine] is a nucleoside analogue with a high and selective antiviral activity against varicella-zoster virus (VZV) and herpes simplex virus type 1 (HSV-1). The double-blind, randomized study presented here compared efficacy and safety of oral brivudin 1 x 125 mg and acyclovir 5 ...

    journal_title:Antiviral research

    pub_type: 临床试验,杂志文章,多中心研究,随机对照试验

    doi:10.1016/s0166-3542(03)00065-2

    authors: Wassilew SW,Wutzler P,Brivddin Herpes Zoster Study Group.

    更新日期:2003-06-01 00:00:00

  • The cooperative effect of interferon-alpha and ribavirin on subacute sclerosing panencephalitis (SSPE) virus infections, in vitro and in vivo.

    abstract::We studied the effects of two antiviral agents, human interferon-alpha (IFN-alpha) and ribavirin, on subacute sclerosing panencephalitis (SSPE) virus infections in hamsters. By intracranial administration, IFN-alpha alone improved the survival of infected hamsters by 20% at a dose of 6 x 10(4) IU/kg every other day fo...

    journal_title:Antiviral research

    pub_type: 杂志文章

    doi:10.1016/s0166-3542(97)00055-7

    authors: Takahashi T,Hosoya M,Kimura K,Ohno K,Mori S,Takahashi K,Shigeta S

    更新日期:1998-01-01 00:00:00

  • Successful strategies implemented towards the elimination of canine rabies in the Western Hemisphere.

    abstract::Almost all cases of human rabies result from dog bites, making the elimination of canine rabies a global priority. During recent decades, many countries in the Western Hemisphere have carried out large-scale dog vaccination campaigns, controlled their free-ranging dog populations and enforced legislation for responsib...

    journal_title:Antiviral research

    pub_type: 历史文章,杂志文章,评审

    doi:10.1016/j.antiviral.2017.03.023

    authors: Velasco-Villa A,Escobar LE,Sanchez A,Shi M,Streicker DG,Gallardo-Romero NF,Vargas-Pino F,Gutierrez-Cedillo V,Damon I,Emerson G

    更新日期:2017-07-01 00:00:00

  • Hepatitis C virus NS3/4A protease.

    abstract::Despite an urgent medical need, a broadly effective anti-viral therapy for the treatment of infections with hepatitis C viruses (HCVs) has yet to be developed. One of the approaches to anti-HCV drug discovery is the design and development of specific small molecule drugs to inhibit the proteolytic processing of the HC...

    journal_title:Antiviral research

    pub_type: 杂志文章,评审

    doi:

    authors: Kwong AD,Kim JL,Rao G,Lipovsek D,Raybuck SA

    更新日期:1999-02-01 00:00:00

  • Inhibition of herpes simplex virus infection by oligomeric stilbenoids through ROS generation.

    abstract::Stilbenoids including resveratrol contain the basic structural unit of 1,2-diphenylethylene. Naturally occurring stilbenoids have broad structural features due to oligomerization and modifications and some have demonstrated potent biological activities. In an effort to identify bioactive stilbenoids, we screened a gro...

    journal_title:Antiviral research

    pub_type: 杂志文章

    doi:10.1016/j.antiviral.2012.05.001

    authors: Chen X,Qiao H,Liu T,Yang Z,Xu L,Xu Y,Ge HM,Tan RX,Li E

    更新日期:2012-07-01 00:00:00

  • Antiviral drug susceptibility assays: going with the flow.

    abstract::This review describes the procedures for the use of fluorochrome labeled monoclonal antibodies and flow cytometry for the detection and quantification of virus infected cells. The application of this technology for (1) identifying virus infected cells in clinical specimens obtained from human cytomegalovirus (HCMV) an...

    journal_title:Antiviral research

    pub_type: 杂志文章,评审

    doi:10.1016/s0166-3542(99)00039-x

    authors: McSharry JJ

    更新日期:1999-08-01 00:00:00

  • Virus and host interactions critical for filoviral RNA synthesis as therapeutic targets.

    abstract::Filoviruses, which include Ebola virus (EBOV) and Marburg virus, are negative-sense RNA viruses associated with sporadic outbreaks of severe viral hemorrhagic fever characterized by uncontrolled virus replication. The extreme virulence and emerging nature of these zoonotic pathogens make them a significant threat to h...

    journal_title:Antiviral research

    pub_type: 杂志文章,评审

    doi:10.1016/j.antiviral.2018.12.006

    authors: Basler CF,Krogan NJ,Leung DW,Amarasinghe GK

    更新日期:2019-02-01 00:00:00

  • Recombinant DNA vaccine of Hantavirus Gn and LAMP1 induced long-term immune protection in mice.

    abstract:BACKGROUND:Prophylaxis is widely adopted the best choice against Hemorrhagic fever with renal syndrome (HFRS) caused by Hantavirus. However, loss of memory immune response maintenance remains as major shortcoming in current HFRS vaccine. A recombinant DNA vaccine, pVAX-LAMP/Gn was previously proved efficient, requiring...

    journal_title:Antiviral research

    pub_type: 杂志文章

    doi:10.1016/j.antiviral.2016.12.001

    authors: Jiang DB,Sun LJ,Cheng LF,Zhang JP,Xiao SB,Sun YJ,Yang SY,Wang J,Zhang FL,Yang K

    更新日期:2017-02-01 00:00:00

  • Suramin inhibits Zika virus replication by interfering with virus attachment and release of infectious particles.

    abstract::Zika virus (ZIKV) is a mosquito-borne flavivirus that mostly causes asymptomatic infections or mild disease characterized by low-grade fever, rash, conjunctivitis, and malaise. However, the recent massive ZIKV epidemics in the Americas have also linked ZIKV infection to fetal malformations like microcephaly and Guilla...

    journal_title:Antiviral research

    pub_type: 杂志文章

    doi:10.1016/j.antiviral.2017.04.016

    authors: Albulescu IC,Kovacikova K,Tas A,Snijder EJ,van Hemert MJ

    更新日期:2017-07-01 00:00:00

  • Anti-herpesvirus activity of citrusinine-I, a new acridone alkaloid, and related compounds.

    abstract::Citrusinine-I, a new acridone alkaloid isolated from the root bark of the citrus plant (Rutaceae), exhibited potent activity against herpes simplex virus (HSV) type 1 and type 2 at low concentrations relative to their cytotoxicity; 50% effective concentrations (ED50) of citrusinine-I were 0.56 micrograms/ml and 0.74 m...

    journal_title:Antiviral research

    pub_type: 杂志文章

    doi:10.1016/0166-3542(89)90065-x

    authors: Yamamoto N,Furukawa H,Ito Y,Yoshida S,Maeno K,Nishiyama Y

    更新日期:1989-08-01 00:00:00

  • Potent antiviral agents fail to elicit genetically-stable resistance mutations in either enterovirus 71 or Coxsackievirus A16.

    abstract::Enterovirus 71 (EV71) and Coxsackievirus A16 (CVA16) are the two major causative agents of hand, foot and mouth disease (HFMD), for which there are currently no licenced treatments. Here, the acquisition of resistance towards two novel capsid-binding compounds, NLD and ALD, was studied and compared to the analogous co...

    journal_title:Antiviral research

    pub_type: 杂志文章

    doi:10.1016/j.antiviral.2015.10.006

    authors: Kelly JT,De Colibus L,Elliott L,Fry EE,Stuart DI,Rowlands DJ,Stonehouse NJ

    更新日期:2015-12-01 00:00:00