Abstract:
:T Follicular helper cells (TFH) are considered critical for B cell antibody response, and recent efforts have focused on promoting TFH in order to enhance vaccine efficacy. We studied the frequency and function of TFH in nasopharynx-associated lymphoid tissues (NALT) from children and adults, and its role in anti-influenza antibody response following stimulation by a live-attenuated influenza vaccine (LAIV) or an inactivated seasonal virus antigen (sH1N1). We further studied whether CpG-DNA promotes TFH and by which enhances anti-influenza response. We showed NALT from children aged 1.5-10 years contained abundant TFH, suggesting efficient priming of TFH during early childhood. Stimulation by LAIV induced a marked increase in TFH that correlated with a strong production of anti-hemagglutinin (HA) IgA/IgG/IgM antibodies in tonsillar cells. Stimulation by the inactivated sH1N1 antigen induced a small increase in TFH which was markedly enhanced by CpG-DNA, accompanied by enhanced anti-HA antibody responses. In B cell co-culture experiment, anti-HA responses were only seen in the presence of TFH, and addition of plasmacytoid dendritic cell to TFH-B cell co-culture enhanced the TFH-mediated antibody production following CpG-DNA and sH1N1 antigen stimulation. Induction of TFH differentiation from naïve T cells was also shown following the stimulation. Our results support a critical role of TFH in human mucosal anti-influenza antibody response. Use of an adjuvant such as CpG-DNA that has the capacity to promote TFH by which to enhance antigen-induced antibody responses in NALT tissue may have important implications for future vaccination strategies against respiratory pathogens.
journal_name
Antiviral Resjournal_title
Antiviral researchauthors
Aljurayyan AN,Sharma R,Upile N,Beer H,Vaughan C,Xie C,Achar P,Ahmed MS,McNamara PS,Gordon SB,Zhang Qdoi
10.1016/j.antiviral.2016.05.021subject
Has Abstractpub_date
2016-08-01 00:00:00pages
122-30eissn
0166-3542issn
1872-9096pii
S0166-3542(16)30129-2journal_volume
132pub_type
杂志文章abstract::Fifty-nine US isolates of HSV-1 and HSV-2 obtained between 1998 and 2004 were tested for sensitivity to the helicase-primase inhibitor, pritelivir (AIC316, BAY 57-1293) by plaque-reduction assay. All isolates, which were collected prior to any clinical use of primase-helicase inhibitors, were sensitive and showed mean...
journal_title:Antiviral research
pub_type: 杂志文章
doi:10.1016/j.antiviral.2013.08.024
更新日期:2013-11-01 00:00:00
abstract::Dengue virus (DENV) is the most prevalent mosquito borne viral pathogen worldwide. In this work we first evaluated the antiviral activity of natural and synthetic β-carbolines against DENV-2 multiplication in cell cultures. We determined that the natural β-carboline harmol and a synthetic harmine derivative, 9N-methyl...
journal_title:Antiviral research
pub_type: 杂志文章
doi:10.1016/j.antiviral.2016.08.018
更新日期:2016-10-01 00:00:00
abstract::(S)-1-(3-hydroxy-2-phosphonylmethoxypropyl)cytosine, HPMPC, and two HPMPC-related nucleoside analogs, (S)-9-(3-hydroxy-2-phosphonylmethoxypropyl)adenine, HPMPA, and (2-phosphonylmethoxyethyl)guanine, PMEG, were evaluated for their antiviral activities against guinea pig cytomegalovirus (GPCMV) infection in guinea pig ...
journal_title:Antiviral research
pub_type: 杂志文章
doi:10.1016/0166-3542(90)90069-j
更新日期:1990-05-01 00:00:00
abstract::The EIAV (equine infectious anemia virus) multi-species attenuated vaccine EIAV(DLV121) successfully prevented the spread of equine infectious anemia (EIA) in China in the 1970s and provided an excellent model for the study of protective immunity to lentiviruses. In this study, we compared immune responses induced by ...
journal_title:Antiviral research
pub_type: 杂志文章
doi:10.1016/j.antiviral.2011.08.016
更新日期:2011-11-01 00:00:00
abstract:UNLABELLED:This paper presents the public health rationale for multipurpose prevention technologies (MPTs) for sexual and reproductive health (SRH) based on regional trends in demographic and SRH indicators. It then distils important lessons gleaned from the introduction of contraceptive and reproductive health product...
journal_title:Antiviral research
pub_type: 杂志文章,评审
doi:10.1016/j.antiviral.2013.09.019
更新日期:2013-12-01 00:00:00
abstract::Conventional treatment of severe viral disease is limited by the narrow choice as well as the often-significant side effects or lack of clear efficacy of antiviral chemotherapy. At the same time, however, it is known that a reduction in viral load leads to significant clinical improvement in a number of important vira...
journal_title:Antiviral research
pub_type: 杂志文章
doi:10.1016/j.antiviral.2010.05.007
更新日期:2010-08-01 00:00:00
abstract::RNase L is a cellular endoribonuclease that is activated by 2',5'-linked oligoadenylates (2-5A), which are unique and specific ligands synthesized by a family of interferon-inducible, dsRNA-activated enzymes named oligoadenylate synthetases. In the typical antiviral pathway, activated RNase L degrades viral and cellul...
journal_title:Antiviral research
pub_type: 杂志文章
doi:10.1016/j.antiviral.2014.01.021
更新日期:2014-04-01 00:00:00
abstract::Dengue virus (DENV) infection is the most prevalent mosquito-borne viral infection of which there is no licensed therapeutic drug available. Previous studies have shown that minocycline, an antibiotic, can inhibit DENV infection in vitro. However, the mechanism is not fully understood. It is known that macrophage migr...
journal_title:Antiviral research
pub_type: 杂志文章
doi:10.1016/j.antiviral.2018.05.002
更新日期:2018-07-01 00:00:00
abstract::The hepatitis B virus nucleocapsid or core antigen is extremely immunogenic during infection and after immunization. This review summarizes several features of the nucleocapsid which explain this exceptionally high immunogenicity: a unique three-dimensional folding, the presence of a region that interacts with immunog...
journal_title:Antiviral research
pub_type: 杂志文章,评审
doi:10.1016/j.antiviral.2003.08.011
更新日期:2003-10-01 00:00:00
abstract::Infection with the oncogenic γ-herpesviruses Epstein-Barr virus (EBV) and Kaposi's sarcoma-associated herpesvirus (KSHV) cause several severe malignancies in humans. Inhibition of the lytic replication of EBV and KSHV eliminates the reservoir of persistent infection and transmission, consequently preventing the occurr...
journal_title:Antiviral research
pub_type: 杂志文章
doi:10.1016/j.antiviral.2016.12.002
更新日期:2017-02-01 00:00:00
abstract::A major challenge in the development of vaccines against retroviruses is the induction of neutralizing antibodies since they prevent infection of the cells where the virus may persist. The transmembrane envelope (TM) protein contains highly conserved domains and seems to be a suitable target. To study whether vaccinat...
journal_title:Antiviral research
pub_type: 杂志文章
doi:10.1016/j.antiviral.2010.11.011
更新日期:2011-01-01 00:00:00
abstract::Ribonucleases H (RNases H) are endonucleolytic enzymes, evolutionarily related to retroviral integrases, DNA transposases, resolvases and numerous nucleases. RNases H cleave RNA in RNA/DNA hybrids and their activity plays an important role in the replication of prokaryotic and eukaryotic genomes, as well as in the rep...
journal_title:Antiviral research
pub_type: 杂志文章,评审
doi:10.1016/j.antiviral.2019.104613
更新日期:2019-11-01 00:00:00
abstract::Favipiravir (T-705) is a broad spectrum antiviral which has been approved in Japan for the treatment of severe influenza virus infections. We reported earlier that favipiravir inhibits the in vitro replication of CHIKV and protects against disease progression in CHIKV-infected immunodeficient mice. We here explored wh...
journal_title:Antiviral research
pub_type: 杂志文章
doi:10.1016/j.antiviral.2017.09.016
更新日期:2018-01-01 00:00:00
abstract::Three water-soluble derivatives of amphotericin B were tested for inhibition of HIV infection in vitro. The compounds amphotericin B methyl ester (AME) and N-(N'-(2-(4'-methylmorpholinio)ethyl)N"-cyclohexyl guanyl) amphotericin B methyl ester (MCG) inhibited HIV infection by 50% at 1 microgram/ml; N-(N'-(3-dimethylami...
journal_title:Antiviral research
pub_type: 杂志文章
doi:10.1016/0166-3542(90)90031-2
更新日期:1990-09-01 00:00:00
abstract::Several polyanionic compounds with potential for use as topically applied microbicides to prevent HIV-1 sexual transmission, such as PRO 2000, are currently in phase III clinical efficacy trials. Microbicidal formulations may well comprise combinations of inhibitors to increase potency, reduce dose and minimize proble...
journal_title:Antiviral research
pub_type: 杂志文章
doi:10.1016/j.antiviral.2007.03.004
更新日期:2007-09-01 00:00:00
abstract::In the event of a bioterror attack with variola virus (smallpox), exposure may only be identified following onset of fever. To determine if antiviral therapy with brincidofovir (BCV; CMX001) initiated at, or following, onset of fever could prevent severe illness and death, a lethal rabbitpox model was used. BCV is in ...
journal_title:Antiviral research
pub_type: 杂志文章
doi:10.1016/j.antiviral.2017.04.002
更新日期:2017-07-01 00:00:00
abstract:BACKGROUND:Cases of sustained-virological response (SVR or cure) after an ultra-short duration (≤27 days) of direct-acting antiviral (DAA)-based therapy, despite HCV being detected at end of treatment (EOT), have been reported. Established HCV mathematical models that predict the treatment duration required to achieve ...
journal_title:Antiviral research
pub_type: 杂志文章
doi:10.1016/j.antiviral.2017.06.019
更新日期:2017-08-01 00:00:00
abstract::The aminoadamantanes, amantadine and rimantadine, have been used to prevent and treat influenza A virus infections for many years. Several reports have shown an increased level of resistance to these drugs, particularly among influenza A(H3N2) subtype viruses, during recent years. We observed an increase in amantadine...
journal_title:Antiviral research
pub_type: 杂志文章
doi:10.1016/j.antiviral.2010.08.013
更新日期:2010-11-01 00:00:00
abstract::The 1-(2-hydroxyethoxymethyl)-6-(phenylthio)thymine (HEPT)-like compounds with homocyclic moieties at the N-1 of the pyrimidinedione, including the highly potent lead compound IQP-0410, inhibit HIV-1 at sub-nanomolar concentrations primarily through a typical non-nucleoside mechanism involving allosteric inhibition at...
journal_title:Antiviral research
pub_type: 杂志文章
doi:10.1016/j.antiviral.2013.09.005
更新日期:2013-11-01 00:00:00
abstract::The family Flaviviridae comprises several major human pathogens including hepatitis C virus (genus hepacivirus), yellow fever virus, West Nile virus and dengue virus (genus flavivirus). Flaviviridae genomes comprise a single-stranded RNA segment encoding a single polyprotein that is subsequently processed into 10 matu...
journal_title:Antiviral research
pub_type: 杂志文章,评审
doi:10.1016/j.antiviral.2014.02.006
更新日期:2014-05-01 00:00:00
abstract::Cell-to-cell contact is usually essential for syncytium formation by HTLV-I-infected cell lines. The present study was undertaken to determine the inhibitory effect of polyanionic compounds, dextran sulfate and heparin, on HTLV-I-induced syncytium formation, as demonstrated by the fusion of HTLV-I-infected cells with ...
journal_title:Antiviral research
pub_type: 杂志文章
doi:10.1016/0166-3542(94)90041-8
更新日期:1994-02-01 00:00:00
abstract::Acute lower respiratory tract infections (ALRI) caused by respiratory syncytial virus (RSV) are associated with a severe disease burden among infants and elderly patients. Treatment options are limited. While numerous drug candidates with different viral targets are under development, the utility of RSV entry inhibito...
journal_title:Antiviral research
pub_type: 杂志文章
doi:10.1016/j.antiviral.2020.104774
更新日期:2020-05-01 00:00:00
abstract::Itraconazole (ITZ) is a well-known, FDA-approved antifungal drug that is also in clinical trials for its anticancer activity. ITZ exerts its anticancer activity through several disparate targets and pathways. ITZ inhibits angiogenesis by hampering the functioning of the vascular endothelial growth receptor 2 (VEGFR2) ...
journal_title:Antiviral research
pub_type: 杂志文章
doi:10.1016/j.antiviral.2018.05.010
更新日期:2018-08-01 00:00:00
abstract::Human CMV causes a number of diseases that cause considerable morbidity and that can be life-threatening in immunocompromised patients, particularly those with AIDS. Ganciclovir (GCV) and Foscarnet (PFA) are currently the drugs of choice for management of CMV disease. Both are not without side effects and have a relat...
journal_title:Antiviral research
pub_type: 杂志文章
doi:10.1016/0166-3542(94)90048-5
更新日期:1994-05-01 00:00:00
abstract::Sulfated derivatives of paramylon, a water-insoluble (1-3)-beta-D-glucan from Euglena gracilis, significantly inhibited the cytopathic effect of human immunodeficiency virus (HIV-1, HIV-2) and the expression of HIV antigen in cultured MT-4, MOLT-4 cells and human peripheral blood mononuclear cells. Native paramylon, N...
journal_title:Antiviral research
pub_type: 杂志文章
doi:10.1016/0166-3542(93)90063-o
更新日期:1993-05-01 00:00:00
abstract::Infection with Junín virus (JUNV) is currently being effectively managed in the endemic region using a combination of targeted vaccination and plasma therapy. However, the long-term sustainability of plasma therapy is unclear and similar resources are not available for other New World arenaviruses. As a result, there ...
journal_title:Antiviral research
pub_type: 杂志文章
doi:10.1016/j.antiviral.2018.07.009
更新日期:2018-09-01 00:00:00
abstract::Innate, non-specific, resistance mechanisms are important barriers to pathogens, particularly delaying virus multiplication at the onset of infections. These innate defense mechanisms include a series of mechanical barriers, pre-existing inhibitory molecules, and cellular responses with antimicrobial activity. The ant...
journal_title:Antiviral research
pub_type: 杂志文章,评审
doi:10.1016/s0166-3542(00)00126-1
更新日期:2000-11-01 00:00:00
abstract::Many aspects of the biology and epidemiology of influenza B viruses are far less studied than for influenza A viruses, and one of these aspects is efficacy and resistance to the clinically available antiviral drugs, the neuraminidase (NA) inhibitors (NAIs). Acute respiratory infections are one of the leading causes of...
journal_title:Antiviral research
pub_type: 杂志文章,评审
doi:10.1016/j.antiviral.2013.08.023
更新日期:2013-11-01 00:00:00
abstract::Rotavirus infection has emerged as an important cause of complications in organ transplantation recipients. Immunosuppressants used to prevent alloreactivity can also interfere with virus infection, but the direct effects of the specific type of immunosuppressants on rotavirus infection are still unclear. Here we prof...
journal_title:Antiviral research
pub_type: 杂志文章
doi:10.1016/j.antiviral.2016.07.017
更新日期:2016-09-01 00:00:00
abstract::For the routine manufacture of live virus vaccines, virus is diluted into a formulation buffer to stabilize it for long-term storage, and to facilitate vaccine administration. The characteristics of this buffer are dependent on the storage temperature of the vaccine, as well as the desired characteristics of the produ...
journal_title:Antiviral research
pub_type: 杂志文章
doi:10.1016/j.antiviral.2005.11.001
更新日期:2006-02-01 00:00:00