Abstract:
:Several novel γ-carboline derivatives were identified as selective inhibitors of bovine viral diarrhea virus (BVDV) replication in cell cultures. Among them, 3,4,5-trimethyl-γ-carboline (SK3M4M5M) was the most active against BVDV (Nose strain) in MDBK cells, with a 50% effective concentration of 0.017±0.005μM and a selectivity index of 435. The compound inhibited viral RNA synthesis in a dose-dependent fashion. In a time of drug-addition experiment during a single viral replication cycle, SK3M4M5M lost its antiviral activity when first added at 8h or later after infection, which coincides with the onset of viral RNA synthesis. When selected γ-carboline derivatives, including SK3M4M5M, were examined for their inhibitory effect on the mutant strains resistant to some classes of nonnucleoside BVDV RNA-dependent RNA polymerase inhibitors, all of which target the top of the finger domain of the polymerase, the strains displayed cross-resistance to the γ-carboline derivatives. These results indicate that the γ-carboline derivatives may possibly target a hot spot of the RNA-dependent RNA polymerase. Although SK3M4M5M was highly active against BVDV, the compound proved inactive against hepatitis C virus (HCV) in HCV RNA replicon cells.
journal_name
Antiviral Resjournal_title
Antiviral researchauthors
Salim MT,Goto Y,Hamasaki T,Okamoto M,Aoyama H,Hashimoto Y,Musiu S,Paeshuyse J,Neyts J,Froeyen M,Herdewijn P,Baba Mdoi
10.1016/j.antiviral.2010.09.013subject
Has Abstractpub_date
2010-12-01 00:00:00pages
263-8issue
3eissn
0166-3542issn
1872-9096pii
S0166-3542(10)00735-7journal_volume
88pub_type
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