Abstract:
:Japanese encephalitis virus (JEV) infection is a major cause of acute viral encephalitis both in humans and animals. The domain III of virus envelope protein (EDIII) plays important roles in interacting with host cell receptors to facilitate virus entry. In this study, recombinant JEV EDIII was expressed and purified. The protein showed the ability to inhibit JEV infection in BHK-21 cells with 50% inhibition at a concentration of 25μg/ml. Based on NMR structure of JEV EDIII, we chose several loop peptides that were reported to be related to receptor binding to test their possible inhibitory activities on virus infection. Our in vitro experiments demonstrated that one of the loop peptides (loop3) can prevent JEV infection with 50% inhibition at concentration of 10μM by interfering in virus attachment to the cells. Our in vivo experiments on mice showed the loop3 was the most protective peptide when administered before virus challenge. Therefore, the loop3 peptide may be served as basis for the development of novel antiviral agents against Japanese encephalitis virus or other flaviviruses infection.
journal_name
Antiviral Resjournal_title
Antiviral researchauthors
Li C,Zhang LY,Sun MX,Li PP,Huang L,Wei JC,Yao YL,Isahg H,Chen PY,Mao Xdoi
10.1016/j.antiviral.2012.03.002subject
Has Abstractpub_date
2012-05-01 00:00:00pages
179-83issue
2eissn
0166-3542issn
1872-9096pii
S0166-3542(12)00060-5journal_volume
94pub_type
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