Abstract:
:Since several different pathways are involved in cerebral ischemia/reperfusion injury, combination therapy rather than monotherapy may be required for efficient neuroprotection. In this study, we examined the protective effects of an apoptosis inhibitor Gly(14)-humanin (HNG) and a necroptosis inhibitor necrostatin-1 (Nec-1) on hypoxia/ischemia/reperfusion injury. Cultured mouse primary cortical neurons were incubated with Nec-1, HNG or both in a hypoxia chamber for 60 min. Cell viability was determined by MTS assay at 24h after oxygen-glucose deprivation (OGD) treatment. Mice underwent middle cerebral artery occlusion for 75 min followed by 24h reperfusion. Mice were administered HNG and/or Nec-1 (i.c.v.) at 4h after reperfusion. Neurological deficits were evaluated and the cerebral infarct volume was determined by TTC staining. Nec-1 or HNG alone had protective effects on OGD-induced cell death. Combined treatment with Nec-1 and HNG resulted in more neuroprotection than Nec-1 or HNG alone. Treatment with HNG or Nec-1 reduced cerebral infarct volume from 59.3 ± 2.6% to 47.0 ± 2.3% and 47.1 ± 1.5%, respectively. Combined treatment with HNG and Nec-1 improved neurological scores and decreased infarct volume to 38.6 ± 1.5%. In summary, we demonstrated that the combination treatment of HNG and Nec-1 conferred synergistic neuroprotection on hypoxia/ischemia/reperfusion injury in vitro and in vivo. These findings provide a novel therapeutic strategy for the treatment of stroke by combining anti-apoptosis and anti-necroptosis therapy.
journal_name
Brain Resjournal_title
Brain researchauthors
Xu X,Chua KW,Chua CC,Liu CF,Hamdy RC,Chua BHdoi
10.1016/j.brainres.2010.07.080subject
Has Abstractpub_date
2010-10-08 00:00:00pages
189-94eissn
0006-8993issn
1872-6240pii
S0006-8993(10)01670-7journal_volume
1355pub_type
杂志文章相关文献
BRAIN RESEARCH文献大全abstract::Local circuit neurons in the human anterior thalamus (AT) were identified on the basis of glutamic acid decarboxylase immunoreactivity (GAD-IR). GAD-IR neurons of the AT displayed small diameter somas with thin, sparsely-branching dendrites, consistent with the morphological characteristics of local circuit neurons fo...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/s0006-8993(01)03194-8
更新日期:2001-12-27 00:00:00
abstract::Antibody has been prepared to purified neurofilament protein extracted from calf brain. This antibody crossreacts with brain and nerve extracts from all mammals tested but fails to crossreact with frog, cod and chicken nerve extracts. Thus the serologic homology of these neurofilament proteins appears to be restricted...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/0006-8993(77)91074-5
更新日期:1977-10-07 00:00:00
abstract::The acute stroke phase is a critical time frame used to evaluate stroke severity, therapeutic options, and prognosis while also serving as a major tool for the development of diagnostics. To further understand stroke pathophysiology and to enhance the development of treatments, our group developed a translational pig ...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/j.brainres.2020.146778
更新日期:2020-06-01 00:00:00
abstract::Natural food sources constitute a promising source of new compounds with neuroprotective properties, once they have the ability to reach the brain. Our aim was to evaluate the brain accessibility of quercetin, epigallocatechin gallate (EGCG) and cyanidin-3-glucoside (C3G) in relation to their neuroprotective capabilit...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/j.brainres.2016.09.020
更新日期:2016-11-15 00:00:00
abstract::We investigated the effect of adenosine A1 receptors on the release of acetylcholine (ACh) and GABA, and on the intracellular calcium concentration ([Ca2+]i) response in cultured chick amacrine-like neurons, stimulated by KCl depolarization. The KCl-induced release of [3H]ACh, but not the release of [14C]GABA, was pot...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/s0006-8993(99)02155-1
更新日期:2000-01-03 00:00:00
abstract::The effects of selective dopamine (DA) depleting lesions with 6-hydroxydopamine microinjection into the SN, CPu, and NAS, as well as radiofrequency lesions of the CPu on the performance characteristics of rats trained on a single-valued 20-s peak-interval (PI) timing procedure or a double-valued 10-s and 60-s PI proce...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/j.brainres.2006.06.031
更新日期:2006-09-13 00:00:00
abstract::Microencephalic rats induced by methylazoxymethanol (MAM) were observed to have notable hyperactivity compared to control rats, as measured by several behavioral parameters in an automated field apparatus. Acute injection of the stimulant drug, methamphetamine (MAP), produced an increase in the incidence of locomotion...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/0006-8993(94)01307-4
更新日期:1995-01-23 00:00:00
abstract::Until now, there has been no effective therapy for chronic sensorineural hearing impairment. This study investigated the role of bone marrow cells (BMCs) in cochlear dysfunction. BALB/c mice (2 months of age), a non-presbycusis-prone mouse strain, were lethally irradiated and then transplanted with BMCs from SAMP1 mic...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/j.brainres.2006.08.074
更新日期:2006-11-20 00:00:00
abstract:BACKGROUND:Diminished dopaminergic neurotransmission contributes to decreased reward and negative eating behaviors in obesity. Bariatric surgery is the most effective therapy for obesity and rapidly reduces hunger and improves satiety through unknown mechanisms. We hypothesized that dopaminergic neurotransmission would...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/j.brainres.2010.03.064
更新日期:2010-09-02 00:00:00
abstract::The monovalent lithium ion inhibits the enzyme myo-inositol-1-phosphatase at concentrations comparable to those which are useful in the treatment of manic depressive illness. However, dialyzed cortical homogenates from rats which have been fed diets containing lithium carbonate demonstrate increased myo-inositol-1-pho...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/0006-8993(86)90245-3
更新日期:1986-08-20 00:00:00
abstract::A growth hormone (GH) injection is able to induce the phosphorylated form of the signal transducer and activator of transcription 5 (pSTAT5) in a large number of cells throughout the mouse brain. The present study had the objective to map the distribution of GH-responsive cells in the brain of rats that received an in...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/j.brainres.2020.147189
更新日期:2021-01-15 00:00:00
abstract::The expressions of mRNAs encoding G protein alpha subunits were analyzed in the cerebral cortex of amygdaloid kindled rats. A remarkable increase in Gsalpha mRNA were observed on the bilateral cerebral cortex at 24 h after the last generalized seizure and persisted 3 weeks on the unstimulated side. Gi2alpha mRNA level...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/s0006-8993(98)01309-2
更新日期:1999-02-13 00:00:00
abstract::Dural afferent neurons are implicated in primary headaches including migraine. Although a significant portion of primary afferent neurons innervating the dura are myelinated A-type neurons, previous electrophysiological studies have primarily characterized the functional properties of small-sized C-type sensory neuron...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/j.brainres.2018.06.007
更新日期:2018-10-01 00:00:00
abstract::Because the occurrence of rapid eye movement (REM) sleep is critically dependent upon an animal's thermal balance, acute pharmacological treatments may effect REM sleep through effects on thermoregulation. We describe how manipulating ambient temperature (T(a)) alters the REM sleep-suppressing effects of systemically-...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/0006-8993(94)90184-8
更新日期:1994-02-04 00:00:00
abstract::Following cholinergic denervation of the hippocampus by medial septal lesions, and unusual neuronal reorganization occurs, in which peripheral sympathetic fibers, originating from the superior cervical ganglia, grow into the hippocampus. Previously, we have found that both hippocampal sympathetic ingrowth (HSI) and ch...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/0006-8993(95)00070-7
更新日期:1995-04-10 00:00:00
abstract::The cytokine interleukin-12 (IL-12) is mainly produced in response to bacterial or parasitic infections. We examined the capacity of mouse brain microglia to release IL-12 forms upon challenge with bacterial lipopolysaccharide (LPS) and studied its modulation by sympathomimetics. LPS evoked the release of IL-12p40 whe...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/s0006-8993(01)02174-6
更新日期:2001-04-27 00:00:00
abstract::The addition of L-glutamic acid (Glu) alone, both Glu and glycine (Gly) or Glu/Gly/spermidine (SPD) was effective in potentiating [3H]5-methyl-10,11-dihydro-5H-dibenzo[a,d]cyclohepten-5,10- imine (MK-801) binding before equilibrium to an ion channel associated with the N-methyl-D-aspartate (NMDA) receptor complex in b...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/0006-8993(94)91029-4
更新日期:1994-02-14 00:00:00
abstract::Pregnenolone sulfate (PS) is an endogenous neurosteroid known to antagonize GABA(A) receptor-mediated inhibitory responses and potentiate NMDA receptor-mediated excitatory responses in vitro. To assess the actions of the steroid as a modulator of seizure susceptibility in vivo, PS (30-300 nmol) was administered intrac...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/s0006-8993(99)01287-1
更新日期:1999-06-12 00:00:00
abstract::Various cytoskeletal proteins have been implicated in the formation of neurofibrillary tangles in Alzheimer's disease. A monoclonal antibody to non-phosphorylated neurofilament protein labels a distinct subset of pyramidal cells in the normal human cortex which have a distribution very similar to that of neurofibrilla...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/0006-8993(87)90914-0
更新日期:1987-07-28 00:00:00
abstract::The impact of hyperglycemia after traumatic brain injury (TBI), and even the administration of glucose-containing solutions to head injured patients, remains controversial. In the current study adult male Sprague-Dawley rats were tested on behavioral tasks and then underwent surgery to induce sham injury or unilateral...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/j.brainres.2015.04.022
更新日期:2015-07-21 00:00:00
abstract::In adult cats, we have investigated how nerve cross-union between slow and fast muscle affects twitch contraction and half-relaxation times in the presence and absence of motor disuse, produced by complete decerebellation. Cerebellectomy abolished the slowing of cross-innervated fast muscle and diminished the amount o...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/0006-8993(82)90480-2
更新日期:1982-02-18 00:00:00
abstract::The human neostriatum was found to contain large neurons (maximum diameter: 30-40 microns) that stain intensely for acetylcholinesterase (AChE). These neurons are few in number, representing less than 5% of the total striatal neuronal population, and appear uniformly scattered throughout the caudate nucleus and putame...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/0006-8993(84)90663-2
更新日期:1984-01-16 00:00:00
abstract::Albino rats received 10 s of sub-seizure electrical stimulation applied to the dentate gyrus granule cells immediately after acquisition of information on trial 1 of a 2-trial radial maze spatial memory task. Granule cell stimulation selectively reduced the probability of accessing information held in declarative memo...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/0006-8993(84)90166-5
更新日期:1984-09-24 00:00:00
abstract::17β-Estradiol is a multi-active steroid that imparts neuroprotection via diverse mechanisms of action. However, its role as a neuroprotective agent after spinal cord injury (SCI), or the involvement of the estrogen receptor-alpha (ER-α) in locomotor recovery, is still a subject of much debate. In this study, we evalua...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/j.brainres.2014.03.002
更新日期:2014-05-02 00:00:00
abstract::In chloralose-urethane anaesthetized cats and in one unanesthetized decerebrate cat, the possibility of a baroreceptor pathway exerting an inhibitory effect directly on the sympathetic preganglionic neurone (SPN) was examined. Stimulation of excitatory points in the brain stem and spinal cord evoked responses in T3 wh...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/0006-8993(81)90212-2
更新日期:1981-09-07 00:00:00
abstract::A histo-enzymatic technique for visualizing and quantifying endogenous NAD(H) in brain tissue was developed, based on coupled enzymatic cycling reactions that reduce nitrotetrazolium blue chloride to produce formazan. Conditions were used where the endogenous level of nicotinamide adenine dinucleotides (NAD(H)) was th...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/j.brainres.2009.12.042
更新日期:2010-02-26 00:00:00
abstract::The effects of VIP and of peptides of the VIP family: secretin, glucagon, the porcine histidine isoleucine containing peptide (PHI) and the rat hypothalamic growth hormone-releasing hormone (rhGRF) on the cyclic AMP and inositol phosphate contents of isolated rat superior cervical ganglia were investigated. We demonst...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/0006-8993(86)90200-3
更新日期:1986-06-25 00:00:00
abstract::In the laboratory rat, inhalation (30 s) of high (> 70%) CO2 concentrations resulted in short-term (1-3 min) anesthesia, followed by a prolonged (up to 60 min) mild antinociception. Exposure to 100% CO2 resulted in significant thermal (hot-plate, 52 degrees, and tail-flick) and mechanical (tail-pinch, 886 g force) ant...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/0006-8993(94)91888-0
更新日期:1994-03-21 00:00:00
abstract::Recently, a novel method for detection of DNA synthesis has been developed based on the incorporation of 5-ethynyl-2'-deoxyuridine (EdU), a thymidine analogue, into cellular DNA and the subsequent reaction of EdU with a fluorescent azide in a copper-catalyzed [3+2] cycloaddition ("Click" reaction). In the present stud...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/j.brainres.2009.12.092
更新日期:2010-03-10 00:00:00
abstract::In anaesthetized cats, medial gastrocnemius motor units (MUs) were stimulated with random sequences (mean rates between 6 and 12 pps) of electrical pulses delivered to their axons in small ventral root filaments. Muscle tension was recorded under isometric conditions, and spike trains of muscle spindle afferents were ...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/0006-8993(86)91656-2
更新日期:1986-02-19 00:00:00