General peroxidase activity of G-quadruplex-hemin complexes and its application in ligand screening.

abstract：:Site-specific mutagenesis has been used to create two mutant versions of aspartate transcarbamoylase. Arg-167 and Gln-231, both previously identified as interacting with the portion of the bisubstrate analogue N-(phosphonoacetyl)-L-aspartate (PALA) that corresponds to aspartate [Krause, K. L., Voltz, K. W., & Lipscomb...

journal_title：Biochemistry

authors： Stebbins JW,Zhang Y,Kantrowitz ER

更新日期：1990-04-24 00:00:00

abstract：:Dopamine (DA) is an unstable neurotransmitter that readily oxidizes to the DA quinone and forms reactive oxygen species, such as superoxide and hydrogen peroxide. The oxidized dopamine also forms thiol conjugates with sulfhydryl groups on cysteine, glutathione, and proteins. In the present study, we determined the red...

journal_title：Biochemistry

authors： Akagawa M,Ishii Y,Ishii T,Shibata T,Yotsu-Yamashita M,Suyama K,Uchida K

更新日期：2006-12-19 00:00:00

abstract：:The bifunctional reagent p-[(bromoacetyl)-amino]phenyl arsenoxide (BrCH2CONHPhAsO) in the presence of excess reduced nicotinamide adenine dinucleotide has been shown to cause the irreversible active site directed inactivation of the lipoamide dehydrogenase (E3) component of the pyruvate dehydrogenase multienzyme (PD) ...

journal_title：Biochemistry

authors： Adamson SR,Stevenson KJ

更新日期：1981-06-09 00:00:00

abstract：:Deposition of misfolded alpha-synuclein is a critical factor in several neurodegenerative disorders. Filamentous alpha-synuclein is the major component of Lewy bodies and Lewy neurites, the intracellular inclusions in the dopaminergic neurons of the substantia nigra, which are considered the pathological hallmark of P...

journal_title：Biochemistry

authors： Munishkina LA,Henriques J,Uversky VN,Fink AL

更新日期：2004-03-23 00:00:00

abstract：:The occupancy of the central cavity of the membrane-embedded c ring of the ATP synthase of Escherichia coli was investigated with a photo-cross-linking approach. Single cysteine mutants were created at c subunit positions 4, 8, and 11, which are oriented to the inside of the ring. These cysteines were alkylated with r...

journal_title：Biochemistry

authors： Oberfeld B,Brunner J,Dimroth P

更新日期：2006-02-14 00:00:00

abstract：:Despite a number of studies, the formation of the Michaelis complexes between ferredoxin-NADP (+) reductases and NADP(H) eluded detailed investigations by rapid kinetic techniques because of their high formation rates. Moreover, the reversible nature of the reaction of hydride ion transfer between these enzymes and NA...

journal_title：Biochemistry

authors： Pennati A,Zanetti G,Aliverti A,Gadda G

更新日期：2008-03-18 00:00:00

abstract：:The EcDos protein belongs to a group of heme-based sensors that detect their ligands with a heme-binding PAS domain. Among these various heme-PAS proteins, EcDos is unique in having its heme iron coordinated at both axial positions to residues of the protein. To achieve its high affinities for ligands, one of the axia...

journal_title：Biochemistry

authors： Gonzalez G,Dioum EM,Bertolucci CM,Tomita T,Ikeda-Saito M,Cheesman MR,Watmough NJ,Gilles-Gonzalez MA

更新日期：2002-07-02 00:00:00

abstract：:The gamma subunits of the 7S nerve growth factor complex (7S NGF) display arginine esteropeptidase activity. By varying the conditions of electrophoresis in acrylamide gel, it has been demonstrated that the gamma-subunit fraction of 7S NGF contains five different proteins, in contrast to the three (gamma1, gamma2, and...

journal_title：Biochemistry

authors： Stach RW,Server AC,Pignatti PF,Piltch A,Shooter EM

更新日期：1976-04-06 00:00:00

abstract：:We have used thrombin to cleave apolipoprotein C-III-1 into two fragments constituting residues 1-40 (apoLP-C-III-A) and 41-79 (apoLP-C-III-B). The lipid binding properties of these fragments with dimyristoyl- and 1-palmitoyl-2-oleoylphosphatidylcholines have been determined using circular dichroic and intrinsic trypt...

journal_title：Biochemistry

authors： Sparrow JT,Pownall HJ,Hsu FJ,Blumenthal LD,Culwell AR,Gotto AM

更新日期：1977-12-13 00:00:00

abstract：:The technique of hydrogen-deuterium exchange coupled to mass spectrometry (HDX-MS) has been applied to a mesophilic (E. coli) dihydrofolate reductase under conditions that allow direct comparison to a thermophilic (B. stearothermophilus) ortholog, Ec-DHFR and Bs-DHFR, respectively. The analysis of hydrogen-deuterium e...

journal_title：Biochemistry

authors： Oyeyemi OA,Sours KM,Lee T,Kohen A,Resing KA,Ahn NG,Klinman JP

更新日期：2011-09-27 00:00:00

abstract：:The X-ray crystal structures of the complexes of the recombinant kringle 1 domain of human plasminogen (Klpg) with the ligands epsilon-aminocaproic acid (EACA) and trans-4-(aminomethyl)cyclohexane-1-carboxylic acid (AMCHA), which are representative of a class of in vivo antifibrinolytic agents, have been determined at...

journal_title：Biochemistry

authors： Mathews II,Vanderhoff-Hanaver P,Castellino FJ,Tulinsky A

更新日期：1996-02-27 00:00:00

abstract：:L6 is a monoclonal antitumor antibody which recognizes an epitope located in a 42-residue extracellular domain of a tumor-associated approximately 22 kDa glycoprotein antigen. The L6 mAb localizes to solid tumors in vivo and triggers complement activation and antibody-dependent cellular cytotoxicity. It has been the s...

journal_title：Biochemistry

authors： Stenzel-Johnson PR,Yelton D,Bajorath J

更新日期：1994-12-06 00:00:00

abstract：:Factor VII (proconvertin) has been purified approximately 5 x 10(5)-fold from bovine plasma with an overall yield of 30%. The isolation procedure involves barium sulfate adsorption and elution, DEAE-Sephadex batchwise adsorption and elution, benzamidine-agarose column chromatography, heparin-agarose column chromatogra...

journal_title：Biochemistry

authors： Kisiel W,Davie EW

更新日期：1975-11-04 00:00:00

abstract：:Bacterial nucleoid-associated proteins (NAPs) are critical to genome integrity and chromosome maintenance. Post-translational modifications of bacterial NAPs appear to function similarly to their better studied mammalian counterparts. The histone-like NAP HupB from Mycobacterium tuberculosis (Mtb) was previously obser...

journal_title：Biochemistry

authors： Green KD,Biswas T,Pang AH,Willby MJ,Reed MS,Stuchlik O,Pohl J,Posey JE,Tsodikov OV,Garneau-Tsodikova S

更新日期：2018-02-06 00:00:00

abstract：:FTIR-difference spectroscopy in combination with site-directed mutagenesis has been used to investigate the role of water during the photocycle of bacteriorhodopsin. At least one water molecule is detected which undergoes an increase in H-bonding during the primary bR-->K phototransition. Bands due to water appear in ...

journal_title：Biochemistry

authors： Fischer WB,Sonar S,Marti T,Khorana HG,Rothschild KJ

更新日期：1994-11-01 00:00:00

abstract：:Among hormone-inducible transcription factors, estrogen receptors (ERs) play important roles in tissue growth and differentiation, via either direct or indirect binding, in the nucleus, to specific DNA targets called estrogen responsive elements (EREs), or through nongenomic pathways. In humans, two estrogen receptor ...

journal_title：Biochemistry

authors： Le Grand A,André-Leroux G,Marteil G,Duval H,Sire O,Le Tilly V

更新日期：2015-06-30 00:00:00

abstract：:Neutral solutes were used to investigate the effects of osmotic stress both on the ability of rhodopsin to undergo its activating conformation change and on acyl chain packing in the rod outer segment (ROS) disk membrane. The equilibrium concentration of metarhodopsin II (MII), the conformation of photoactivated rhodo...

journal_title：Biochemistry

authors： Mitchell DC,Litman BJ

更新日期：1999-06-15 00:00:00

abstract：:Porcine aortic myosin is a smooth muscle contractile protein similar to its striated muscle counterpart. Electrophoresis in sodium dodecyl sulfate indicates that the molecule consists of three classes of subunits with polypeptide chain molecular weights of 192,000, 19,000, and 15,000. At 277 nm the absorption spectrum...

journal_title：Biochemistry

authors： Frederiksen DW

更新日期：1979-05-01 00:00:00

abstract：:The transporter associated with antigen processing (TAP) is a prototype of an asymmetric ATP-binding cassette (ABC) transporter, which uses ATP binding and hydrolysis to translocate peptides from the cytosol to the lumen of the endoplasmic reticulum (ER). Here, we review molecular details of peptide binding and ATP bi...

journal_title：Biochemistry

authors： Hinz A,Tampé R

更新日期：2012-06-26 00:00:00

abstract：:The direct interrogation of fleeting intermediates by rapid-mixing kinetic methods has significantly advanced our understanding of enzymes that utilize dioxygen. The gas's modest aqueous solubility (<2 mM at 1 atm) presents a technical challenge to this approach, because it limits the rate of formation and extent of a...

journal_title：Biochemistry

authors： Dassama LM,Yosca TH,Conner DA,Lee MH,Blanc B,Streit BR,Green MT,DuBois JL,Krebs C,Bollinger JM Jr

更新日期：2012-02-28 00:00:00

abstract：:The association between the papillomavirus E1 and E2 proteins is an important regulatory interaction, imparting coordinated control of viral transcription and replication. Using fluorescence polarization, we have characterized the interactions between HPV-11 E1, HPV-11 E2, and DNA in solution at equilibrium. For these...

journal_title：Biochemistry

authors： Chao SF,Rocque WJ,Daniel S,Czyzyk LE,Phelps WC,Alexander KA

更新日期：1999-04-06 00:00:00

abstract：:Two major neutral glyceroglucolipids (A and B) have been isolated from lipid extract of human gastric content by the procedure involving column fractionation on DEAE-Sephadex, silicic acid, and thin-layer chromatography. Both glycolipids contained glucose, glyceryl ethers, and fatty acids. The structures of these glyc...

journal_title：Biochemistry

authors： Slomiany BL,Slomiany A,Glass GB

更新日期：1977-09-06 00:00:00

abstract：:The behavior of fluorescent and spin-label probes is examined in several fluid and gel phospholipid phases, with particular focus on the Ca2+-induced gel phase in phosphatidylserine (PS). These probes have behavior characteristic of the type of probe and of the type of lipid environment. Anthroyloxy- and doxyl-labeled...

journal_title：Biochemistry

authors： Florine KI,Feigenson GW

更新日期：1987-03-24 00:00:00

abstract：:To elucidate a unique mechanism for the quinol oxidation in the Escherichia coli cytochrome bo, we applied pulse radiolysis technique to the wild-type enzyme with or without a single bound ubiquinone-8 at the high-affinity quinone binding site (Q(H)), using N-methylnicotinamide (NMA) as an electron mediator. With the ...

journal_title：Biochemistry

authors： Kobayashi K,Tagawa S,Mogi T

更新日期：2000-12-19 00:00:00

abstract：:There is increasing evidence that a class of cell membrane glycolipids, gangliosides, can mediate the fibrillogenesis and toxicity of Alzheimer's disease amyloid-beta peptide (Abeta). Using lipid monolayers and vesicles as model membranes, we measured the insertion of Abeta into 1,2-dipalmitoyl-sn-glycero-3-phosphocho...

journal_title：Biochemistry

authors： Chi EY,Frey SL,Lee KY

更新日期：2007-02-20 00:00:00

abstract：:Urea-induced unfolding of Na,K-ATPase from pig kidney and from shark salt gland was studied by electron paramagnetic resonance (EPR) spectroscopy of a nitroxyl derivative of maleimide covalently attached to sulfhydryl groups which are essential for activity. Urea-induced structural changes lead to the inhibition of Na...

journal_title：Biochemistry

authors： Babavali M,Esmann M,Fedosova NU,Marsh D

更新日期：2009-09-29 00:00:00

abstract：:Apolipoprotein B (apoB) is a nonexchangeable apolipoprotein that dictates the synthesis of chylomicrons and very low density lipoproteins. ApoB is the major protein in low density lipoprotein, also known as the "bad cholesterol" that is directly implicated in atherosclerosis. It has been suggested that the N-terminal ...

journal_title：Biochemistry

authors： Jiang ZG,Gantz D,Bullitt E,McKnight CJ

更新日期：2006-10-03 00:00:00

abstract：:Phosphotriesterase homology protein (PHP) is a member of a recently discovered family of proteins related to phosphotriesterase, a hydrolytic, bacterial enzyme with an unusual substrate specificity for synthetic organophosphate triesters and phosphorofluoridates, which are common constituents of chemical warfare agent...

journal_title：Biochemistry

authors： Buchbinder JL,Stephenson RC,Dresser MJ,Pitera JW,Scanlan TS,Fletterick RJ

更新日期：1998-04-14 00:00:00

abstract：:Wheat germ ribosomes combine with the AUG codon at positions 30-32 from the 5' terminus of in vitro radioiodinated satellite tobacco necrosis virus (STNV) ribonucleic acid (RNA) to form initiation complexes that protect specific regions of the RNA from attack by ribonucleases. Wheat gern 80S ribosomes convey partial p...

journal_title：Biochemistry

authors： Browning KS,Leung DW,Clark JM Jr

更新日期：1980-05-13 00:00:00

abstract：:Murine class alpha glutathione S-transferase subunit types A2 (mGSTA2-2) and A1 (mGSTA1-1) have high catalytic efficiency for glutathione (GSH) conjugation of the ultimate carcinogenic metabolite of benzo[a]pyrene, (+)-anti-7,8-dihydroxy-9,10-oxy-7,8,9,10-tetrahydrobenzo[a]pyrene, [(+)-anti-BPDE]. Only 10 residues dif...

journal_title：Biochemistry

authors： Gu Y,Xiao B,Wargo HL,Bucher MH,Singh SV,Ji X

更新日期：2003-02-04 00:00:00