Abstract:
:The transporter associated with antigen processing (TAP) is a prototype of an asymmetric ATP-binding cassette (ABC) transporter, which uses ATP binding and hydrolysis to translocate peptides from the cytosol to the lumen of the endoplasmic reticulum (ER). Here, we review molecular details of peptide binding and ATP binding and hydrolysis as well as the resulting allosteric cross-talk between the nucleotide-binding domains and the transmembrane domains that drive translocation of the solute across the ER membrane. We also discuss the general molecular architecture of ABC transporters and demonstrate the importance of structural and functional studies for a better understanding of the role of the noncanonical site of asymmetric ABC transporters. Several aspects of peptide binding and specificity illustrate details of peptide translocation by TAP. Furthermore, this ABC transporter forms the central part of the major histocompatibility complex class I (MHC I) peptide-loading machinery. Hence, TAP is confronted with a number of viral factors, which prevent antigen translocation and MHC I loading in virally infected cells. We review how these viral factors have been used as molecular tools to decipher mechanistic aspects of solute translocation and discuss how they can help in the structural analysis of TAP.
journal_name
Biochemistryjournal_title
Biochemistryauthors
Hinz A,Tampé Rdoi
10.1021/bi300128fsubject
Has Abstractpub_date
2012-06-26 00:00:00pages
4981-9issue
25eissn
0006-2960issn
1520-4995journal_volume
51pub_type
杂志文章,评审相关文献
BIOCHEMISTRY文献大全abstract::Cytochrome c6 from Monoraphidium braunii, an 89-amino acid electron transfer protein, has been investigated by NMR in solution, in its oxidized form, at pH 7 and 300 K. By using a combination of COSY, TOCSY, and NOESY experiments, 84% of the proton resonances have been assigned. A total of 1668 experimental NOE constr...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi972765y
更新日期:1998-04-07 00:00:00
abstract::An IscA homologue within the nif regulon of Azotobacter vinelandii, designated (Nif)IscA, was expressed in Escherichia coli and purified to homogeneity. Purified (Nif)IscA was found to be a homodimer of 11-kDa subunits that contained no metal centers or other prosthetic groups in its as-isolated form. Possible roles f...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi015656z
更新日期:2001-11-20 00:00:00
abstract::Cryptophycin-52 (LY355703) is currently undergoing clinical evaluation for cancer chemotherapy. It is a potent suppressor of microtubule dynamics in vitro, and low picomolar concentrations appear to inhibit cancer cell proliferation at mitosis by stabilizing spindle microtubules. In the present study, using [(3)H]cryp...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi0010827
更新日期:2000-11-21 00:00:00
abstract::In two recent papers, we reported the effects of several point mutations on the thermodynamics of the thermal unfolding of the lysozyme of phage T4 as determined by differential scanning calorimetry. The mutants studied were R96H [Kitamura, S., & Sturtevant, J.M. (1989) Biochemistry 28, 3788-3792] and T157 replaced by...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00121a009
更新日期:1992-02-18 00:00:00
abstract::The influenza M2 protein is the target of the amantadine family of antiviral drugs, and its transmembrane (TM) domain structure and dynamics have been extensively studied. However, little is known about the structure of the highly conserved N-terminal ectodomain, which contains epitopes targeted by influenza vaccines....
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/acs.biochem.6b00727
更新日期:2016-09-27 00:00:00
abstract::Propeptide processing occurs in specific compartments of the secretory pathway, but how these processing-competent organelles are generated from their processing-incompetent precursor compartments is unknown. To dissect the process biochemically, we have developed a novel cell-free system reconstituting the production...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi0112762
更新日期:2001-10-30 00:00:00
abstract::The first β-lactone synthetase enzyme is reported, creating an unexpected link between the biosynthesis of olefinic hydrocarbons and highly functionalized natural products. The enzyme OleC, involved in the microbial biosynthesis of long-chain olefinic hydrocarbons, reacts with syn- and anti-β-hydroxy acid substrates t...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/acs.biochem.6b01199
更新日期:2017-01-17 00:00:00
abstract::Muscarinic cholinergic receptors can appear to be more numerous when labeled by [(3)H]quinuclidinylbenzilate (QNB) than by N-[(3)H]methylscopolamine (NMS). The nature of the implied heterogeneity has been studied with M(2) receptors in detergent-solubilized extracts of porcine atria. The relative capacity for [(3)H]NM...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi011746s
更新日期:2002-04-30 00:00:00
abstract::Kinetic analysis and modeling studies of HIV-1 and HIV-2 proteinases were carried out using the oligopeptide substrate [formula: see text] and its analogs containing single amino acid substitutions in P3-P3' positions. The two proteinases acted similarly on the substrates except those having certain hydrophobic amino ...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00135a008
更新日期:1992-05-26 00:00:00
abstract::Development of potent and isoform selective nitric oxide synthase (NOS) inhibitors is challenging because of the structural similarity in the heme active sites. One amino acid difference between NOS isoforms, Asp597 in rat neuronal NOS (nNOS) versus Asn368 in bovine endothelial NOS (eNOS), has been identified as the s...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/acs.biochem.6b00261
更新日期:2016-07-05 00:00:00
abstract::The effects of an inhibitory monoclonal antibody (mAb) raised against the beta subunit of the Escherichia coli RNA polymerase were determined on the kinetics and structural interactions during formation of the open promoter complex (RPo). Analysis of the kinetics of abortive initiation on linear and supercoiled templa...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00409a058
更新日期:1988-05-03 00:00:00
abstract::Alginate is a heteropolysaccharide that consists of β-D-mannuronate (M) and α-L-guluronate (G). The Gram-negative bacterium Sphingomonas sp. A1 directly incorporates alginate into the cytoplasm through the periplasmic solute-binding protein (AlgQ1 and AlgQ2)-dependent ABC transporter (AlgM1-AlgM2/AlgS-AlgS). Two bindi...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi300194f
更新日期:2012-05-01 00:00:00
abstract::The second-order electron transfer reaction between cytochrome b5 and cytochrome c has been studied by cyclic voltammetry utilizing a gold electrode modified with beta-mercaptopropionate. When cyclic voltammetry is performed on a solution containing a mixture of cytochrome b5, cytochrome c and polylysine, cytochrome b...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi9529723
更新日期:1996-09-24 00:00:00
abstract::Phthalate dioxygenase from Pseudomonas cepacia contains a mononuclear ferrous center that is strictly required for catalytic oxygen activation. The spectroscopic characterization of this iron site and its ligand interactions has been complicated in the past by interference from a Rieske-type binuclear (2Fe-2S) cluster...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00069a017
更新日期:1993-05-11 00:00:00
abstract::G-protein-coupled receptors (GPCRs) represent the largest class of drug targets, accounting for more than 40% of marketed drugs; however, discovery efforts for many GPCRs have failed to provide viable drug candidates. Historically, drug discovery efforts have focused on developing ligands that act at the orthosteric s...
journal_title:Biochemistry
pub_type: 杂志文章,评审
doi:10.1021/bi200129s
更新日期:2011-04-05 00:00:00
abstract::The cellular site of formation, Galpha-coupling preference, and agonist regulation of mu-delta opioid receptor (OR) heterooligomers were studied. Bioluminescence resonance energy transfer (BRET) showed that mu-deltaOR heterooligomers, composed of preformed mu and delta homooligomers, interacted constitutively in the e...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi701436w
更新日期:2007-11-13 00:00:00
abstract::The crystal structures of metyrapone- and 1-, 2-, and 4-phenylimidazole-inhibited complexes of cytochrome P-450cam have been refined to a nominal resolution of 2.1 A and compared with the 1.63-A camphor-bound structure. With the exception of 2-phenylimidazole, each of the inhibitors forms an N-Fe bond with the heme ir...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00399a022
更新日期:1987-12-15 00:00:00
abstract::Phosphorylation by ATP of E.*Ca2 (sarcoplasmic reticulum vesicles (SRV) with bound 45Ca2+) during 5-10 ms leads to the occlusion of 2 *Ca2+/EPtot [quench by ethylene glycol bis(beta-aminoethyl ether)-N,N,N',N'-tetraacetic acid (EGTA) alone] in both "empty" (10 microM free Ca2+in) or "loaded" SRV (20-40 mM free Ca2+in)...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00408a041
更新日期:1988-04-19 00:00:00
abstract::The lipase from Staphylococcus hyicus (SHL) displays a high phospholipase activity whereas the homologous S. aureus lipase (SAL) is not active or hardly active on phospholipid substrates. Previously, it has been shown that elements within the region comprising residues 254-358 are essential for the recognition of phos...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi990096d
更新日期:1999-07-20 00:00:00
abstract::The catalytic utilization of dimethylallyl, geranyl, farnesyl, and geranylgeranyl diphosphates in the reaction catalyzed by recombinant human farnesyl:protein transferase (hFPTase) has been examined in the presence of three different protein substrates, Ras-CVLS, Ras-CVIM, and Ras-CAIL. hFPTase catalyzed both farnesyl...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00083a038
更新日期:1993-08-17 00:00:00
abstract::The complete amino acid sequence of the major component myoglobin from the dwarf sperm whale, Kogia simus, was determined by specific cleavage of the protein to obtain large peptides which are readily degraded by the automatic sequenator. Three easily separable peptides were obtained by cleaving the protein at its two...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00624a010
更新日期:1977-03-08 00:00:00
abstract::A series of cysteine-bearing hydrophobic polypeptides analogous to a light-harvesting one betapolypeptide (LH1beta) from the LH1 complex from the purple photosynthetic bacterium, Rhodobacter sphaeroides, was synthesized using an Escherichia coli expression system. The cysteine was placed in the C- or N-terminal region...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi0478493
更新日期:2005-04-05 00:00:00
abstract::The structure of octylcarbamoyl-alpha-chymotrypsin to a resolution of 3.0 A is described. The n-octyl side chain of the active site directed irreversible inactivator octyl isocyanate is bound exclusively in the hydrophobic substrate binding pocket. The n-octyl isocyanate forms a planar urethane bond with the Ser-195 O...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00694a008
更新日期:1975-11-18 00:00:00
abstract::Diethyl pyrocarbonate (DEP) is an electrophilic reagent that is used to modify reversibly the histidine residues of proteins. Unfortunately, the lability of the acylated histidine adduct usually does not permit the isolation and identification of the modified histidine. By use of 500-MHz proton NMR spectroscopy, it ha...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00445a004
更新日期:1989-09-19 00:00:00
abstract::The 19-transmembrane multisubunit γ-secretase complex generates the amyloid β-peptide (Aβ) of Alzheimer's disease (AD) by intramembrane proteolysis of the β-amyloid precursor protein (APP). Despite substantial advances in elucidating how this protein complex functions, the effect of the local membrane lipid microenvir...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi300303g
更新日期:2012-05-01 00:00:00
abstract::Cytochrome b(561) in adrenal chromaffin vesicle membranes conveys electron equivalents from extravesicular ascorbate to the intravesicular monodehydroascorbate radical. We conducted a stopped-flow study on the reaction of ascorbate with purified cytochrome b(561) in the detergent-solubilized state for the first time. ...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi0267588
更新日期:2003-07-15 00:00:00
abstract::The calcium-dependent binding of two synthetic 18-residue peptides derived from the calmodulin binding region of skeletal myosin light chain kinase to wild-type Drosophila melanogaster calmodulin and four calcium binding site calmodulin mutants has been investigated using optical spectroscopy. The WFF peptide (with W4...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00007a001
更新日期:1995-02-21 00:00:00
abstract::Human liver aldehyde dehydrogenase isozymes E1 and E2 (EC 1.2.1.3) are both completely and irreversibly inactivated by bromoacetophenone (2-bromo-1-phenylethanone). Steady-state kinetics with both acetophenone and chloroacetophenone indicated interaction with the same enzyme form as the aldehyde substrate. Saturation ...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00366a030
更新日期:1986-09-09 00:00:00
abstract::Fluorescence studies of the binding of IKe gene 5 protein to various polynucleotides were performed to obtain insight into the question as to what extent the binding characteristics of the gene 5 proteins of the IKe and M13 phages resemble and/or differ from each other. The fluorescence of IKe gene 5 protein is quench...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00381a037
更新日期:1987-04-07 00:00:00
abstract::We report the use of electrospray ionization (ESI) mass spectrometry (MS) in conjunction with online rapid mixing to monitor the kinetics of acid-induced ferrihemoglobin denaturation. Under equilibrium conditions, the hemoglobin mass spectrum is dominated by the intact heterotetramer. Dimeric and monomeric species are...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi048501a
更新日期:2004-11-23 00:00:00