Acetylation by Eis and Deacetylation by Rv1151c of Mycobacterium tuberculosis HupB: Biochemical and Structural Insight.

abstract：:The nuclei and chromatin of rat liver contain three major proteins reacting with diisopropyl fluorophosphate (DFP). The molecular weights of the three proteins determined by gel filtration in the presence of sodium dodecyl sulfate and sodium dodecyl sulfate-polyacrylamide gel electrophoresis are 70000, 60000, and 250...

journal_title：Biochemistry

authors： Carter DB,Efird PH,Chae CB

更新日期：1976-06-15 00:00:00

abstract：:By limited proteolysis of a type kappa immunoglobulin light chain (Oku) with clostripain, both the VL and CL fragments were obtained with a high yield. The unfolding and refolding by guanidine hydrochloride of light chain Oku and its VL and CL fragments were studied at pH 7.5 and 25 degrees C with circular dichroism a...

journal_title：Biochemistry

authors： Tsunenaga M,Goto Y,Kawata Y,Hamaguchi K

更新日期：1987-09-22 00:00:00

abstract：:In this communication, we present data which describe optimum conditions for reverse transcription of large ribonucleic acid (RNA) templates into deoxyribonucleic acid (DNA) transcripts by the avian retrovirus reverse transcriptase in vitro. In contrast to previous studies, we have optimized all of the reaction compon...

journal_title：Biochemistry

authors： Retzel EF,Collett MS,Faras AJ

更新日期：1980-02-05 00:00:00

abstract：:We consider the problem of color regulation in visual pigments for both bovine rhodopsin (lambda max = 500 nm) and octopus rhodopsin (lambda max = 475 nm). Both pigments have 11-cis-retinal (lambda max = 379 nm, in ethanol) as their chromophore. These rhodopsins were bleached in their native membranes, and the opsins ...

journal_title：Biochemistry

authors： Koutalos Y,Ebrey TG,Tsuda M,Odashima K,Lien T,Park MH,Shimizu N,Derguini F,Nakanishi K,Gilson HR

更新日期：1989-03-21 00:00:00

abstract：:p-Cresol methylhydroxylase (PCMH) isolated from Pseudomonas putida is an alpha 2 beta 2 tetramer of approximate subunit Mr 49,000 and 9,000. It is a flavocytochrome c containing covalently bound FAD in the larger subunit and covalently bound heme in the smaller. Crystals in space group P2(1)2(1)2(1) with unit-cell par...

journal_title：Biochemistry

authors： Mathews FS,Chen ZW,Bellamy HD,McIntire WS

更新日期：1991-01-08 00:00:00

abstract：:In the ATP-dependent carboxylation of biotin that is catalyzed by most biotin-dependent carboxylases, a fundamental mechanistic question is whether the ATP activates bicarbonate (via the formation of carboxyphosphate as an intermediate) or whether the ATP activates biotin (via the formation of O-phosphobiotin). We hav...

journal_title：Biochemistry

authors： Ogita T,Knowles JR

更新日期：1988-10-18 00:00:00

abstract：:Biochemical properties of a detergent-solubilized tetrodotoxin binding component from Electrophorus electricus have been examined and compared with those found for the membrane-bound protein. The toxin binding component was solubilized with high efficiency by a variety of nonionic detergents and with lower efficiency ...

journal_title：Biochemistry

authors： Moore AC,Agnew WS,Raftery MA

更新日期：1982-11-23 00:00:00

abstract：:Polyisoprenyl diphosphates play diverse and vital roles in cell function in health and disease. The counter-regulatory lipid signaling molecule, presqualene diphosphate (PSDP), is rapidly converted to its monophosphate form (PSMP) upon cell activation [Levy, B. D., Petasis, N. A., and Serhan, C. N. (1997) Nature 389, ...

journal_title：Biochemistry

authors： Carlo T,Petasis NA,Levy BD

更新日期：2009-04-07 00:00:00

abstract：:Guanidine hydrochloride (GdnHCl) and thermally induced unfolding measurements on the oxidized form of Escherichia coli thioredoxin at pH 7 were combined for the purpose of assessing the functional dependence of unfolding free energy changes on denaturant concentration over an extended GdnHCl concentration range. Conve...

journal_title：Biochemistry

authors： Santoro MM,Bolen DW

更新日期：1992-05-26 00:00:00

abstract：:The fidelity of the poliovirus RNA-dependent RNA polymerase (3D(pol)) plays a direct role in the genomic evolution and pathogenesis of the virus. A single site mutation (Gly64Ser) that is remote from the catalytic center results in a higher fidelity polymerase. NMR studies with [methyl-(13)C]methionine-labeled protein...

journal_title：Biochemistry

authors： Yang X,Welch JL,Arnold JJ,Boehr DD

更新日期：2010-11-02 00:00:00

abstract：:Homology modeling was used to identify two particular residues, Glu175 and Ala176, in Pseudomonas stutzeri phosphite dehydrogenase (PTDH) as the principal determinants of nicotinamide cofactor (NAD(+) and NADP(+)) specificity. Replacement of these two residues by site-directed mutagenesis with Ala175 and Arg176 both s...

journal_title：Biochemistry

authors： Woodyer R,van der Donk WA,Zhao H

更新日期：2003-10-14 00:00:00

abstract：:The amide hydrogens that are exposed to solvent in the high-resolution X-ray structures of ubiquitin, FK506-binding protein, chymotrypsin inhibitor 2, and rubredoxin span a billion-fold range in hydroxide-catalyzed exchange rates which are predictable by continuum dielectric methods. To facilitate analysis of transien...

journal_title：Biochemistry

authors： LeMaster DM,Anderson JS,Hernández G

更新日期：2009-10-06 00:00:00

abstract：:Database searches indicated that the genome of Bacillus subtilis contains three different genes encoding RNase H homologues. The ypdQ gene encodes an RNase HI homologue with 132 amino acid residues, whereas the rnh and ysgB genes encode RNase HII homologues with 255 and 313 amino acid residues, respectively. RNases HI...

journal_title：Biochemistry

authors： Ohtani N,Haruki M,Morikawa M,Crouch RJ,Itaya M,Kanaya S

更新日期：1999-01-12 00:00:00

abstract：:A procedure for the preparation of coupling factor 1 (F1) from Escherichia coli lacking subunits delta and epsilon is described. Using chloroform and dimethyl sulfoxide, we can isolate F1 containing only subunits alpha, beta, and gamma [F1(alpha beta gamma)] directly from membrane vesicles in 10-mg quantities. Pure an...

journal_title：Biochemistry

authors： Tuttas-Dörschug R,Hanstein WG

更新日期：1989-06-13 00:00:00

abstract：:CRISPR-Cas9 technology has been at the forefront of the field of biology. The Streptococcus pyogenes (SpyCas9) protein forms a complex with guide RNA and can recognize and cleave double-stranded DNA through hybridization based on 20 base pairings. SpyCas9 has two nuclease domains, HNH and RuvC, each of which cuts each...

journal_title：Biochemistry

authors： Furuhata Y,Kato Y

更新日期：2021-01-26 00:00:00

abstract：:Light chain amyloidoses arise from the overproduction and abnormal deposition of the immunoglobulin light chain in various organs. LEN is the variable domain of an immunoglobulin light chain originally isolated from the urine of a patient suffering from multiple myeloma, with no sign of renal dysfunction or amyloidosi...

journal_title：Biochemistry

authors： Hu D,Qin Z,Xue B,Fink AL,Uversky VN

更新日期：2008-08-19 00:00:00

abstract：:The mechanisms that control N protein dependent antitermination in phage lambda have counterparts in many eukaryotic systems, including specific regulatory interactions of the antitermination protein with the nascent RNA transcript. Here we describe the specific and nonspecific RNA binding modes of antitermination pro...

journal_title：Biochemistry

authors： Van Gilst MR,Rees WA,Das A,von Hippel PH

更新日期：1997-02-11 00:00:00

abstract：:Complement components C5b-6 and C7 assemble to form C5b-7, which then interacts with membranes and commits the membrane attack complex to a target site. This protein-membrane association event was investigated to determine possible structural features that could contribute to a selective membrane attack. This system m...

journal_title：Biochemistry

authors： Silversmith RE,Nelsestuen GL

更新日期：1986-11-18 00:00:00

abstract：:Among the major obstacles to pharmacological and structural studies of integral membrane proteins (MPs) are their natural scarcity and the difficulty in overproducing them in their native form. MPs can be overexpressed in the non-native state as inclusion bodies, but inducing them to achieve their functional three-dim...

journal_title：Biochemistry

authors： Pocanschi CL,Dahmane T,Gohon Y,Rappaport F,Apell HJ,Kleinschmidt JH,Popot JL

更新日期：2006-11-28 00:00:00

abstract：:The ubiquitin signaling pathway consists of hundreds of enzymes that are tightly regulated for the maintenance of cell homeostasis. Parkin is an E3 ubiquitin ligase responsible for conjugating ubiquitin onto a substrate protein, which itself can be ubiquitinated. Ataxin-3 performs the opposing function as a deubiquiti...

journal_title：Biochemistry

authors： Bai JJ,Safadi SS,Mercier P,Barber KR,Shaw GS

更新日期：2013-10-22 00:00:00

abstract：:The structures of the modified folates present in Pyrococcus furiosus have been determined. This was accomplished largely by the characterization of the arylamines resulting from the air oxidative cleavage of the reduced modified folates present in these cells, using both chemical and enzymatic methods. Cell extracts ...

journal_title：Biochemistry

authors： White RH

更新日期：1993-01-26 00:00:00

abstract：:Casein kinase II is a widely distributed protein serine/threonine kinase. The holoenzyme appears to be a tetramer, containing two alpha or alpha' subunits (or one of each) and two beta subunits. Complementary DNA clones encoding the subunits of casein kinase II were isolated from a human T-cell lambda gt10 library usi...

journal_title：Biochemistry

authors： Lozeman FJ,Litchfield DW,Piening C,Takio K,Walsh KA,Krebs EG

更新日期：1990-09-11 00:00:00

abstract：:The Type I homodimeric photosynthetic reaction center found in anaerobic gram-positive bacteria of the genus Heliobacteriaceae incorporates FA- and FB-like iron-sulfur clusters similar to those found in Photosystem I as terminal electron acceptors. We recently isolated the PshB protein that harbors the iron-sulfur clu...

journal_title：Biochemistry

authors： Heinnickel M,Shen G,Golbeck JH

更新日期：2007-03-06 00:00:00

abstract：:Despite the high degree of sequence similarity in their basic-helix-loop-helix (BHLH) domains, MASH-1 and MyoD are involved in different biological processes. In order to define possible differences between the DNA binding specificities of these two proteins, we investigated the DNA binding properties of MASH-1 by cir...

journal_title：Biochemistry

authors： Meierhan D,el-Ariss C,Neuenschwander M,Sieber M,Stackhouse JF,Allemann RK

更新日期：1995-09-05 00:00:00

abstract：:In an effort to further probe metal binding to metallo-beta-lactamase L1 (mbetal L1), Cu- (Cu-L1) and Ni-substituted (Ni-L1) L1 were prepared and characterized by kinetic and spectroscopic studies. Cu-L1 bound 1.7 equiv of Cu and small amounts of Zn(II) and Fe. The EPR spectrum of Cu-L1 exhibited two overlapping, axia...

journal_title：Biochemistry

authors： Hu Z,Spadafora LJ,Hajdin CE,Bennett B,Crowder MW

更新日期：2009-04-07 00:00:00

abstract：:The ATP hydrolysis and protein-binding and release cycle of the molecular chaperone DnaK is regulated by the accessory proteins GrpE and DnaJ. Here we describe a study of the formation of complexes between the molecular chaperone DnaK, its nucleotide exchange factor GrpE, and the fluorescent ADP analog N8-[4-[(N'-meth...

journal_title：Biochemistry

authors： Packschies L,Theyssen H,Buchberger A,Bukau B,Goody RS,Reinstein J

更新日期：1997-03-25 00:00:00

abstract：:The interaction of linker histone H1 with both linear and superhelical double-stranded DNA has been investigated at low ionic strengths. Gel mobility retardation experiments demonstrate strikingly different behavior for the two forms of DNA. First, the experiments strongly suggest that linker histone binds to superhel...

journal_title：Biochemistry

authors： Ellen TP,van Holde KE

更新日期：2004-06-22 00:00:00

abstract：:The contributions to thermostability of interactions within the beta-sheet region of rubredoxins (Rds) were investigated by examining proteins in which beta-strand sequences of Rds from the hyperthermophilic archaeon Pyrococcus furiosus (Pf) and the mesophilic bacterium Clostridium pasteurianum (Cp) were interchanged....

journal_title：Biochemistry

authors： Eidsness MK,Richie KA,Burden AE,Kurtz DM Jr,Scott RA

更新日期：1997-08-26 00:00:00

abstract：:The thermodynamic binding parameters and crystal structure for streptavidin-peptide complexes where the peptide sequences were obtained by random screening methods are reported. The affinities between streptavidin and two heptapeptides were determined by titrating calorimetric methods [Phe-Ser-His-Pro-Gln-Asn-Thr, Ka ...

journal_title：Biochemistry

authors： Weber PC,Pantoliano MW,Thompson LD

更新日期：1992-10-06 00:00:00

abstract：:The product of transesterification of phospholipid acyl chains and unesterified cholesterol (UC) by the enzyme lecithin:cholesterol acyltransferase (LCAT) is cholesteryl ester (CE). Activation of LCAT by apolipoprotein (apo) A-I on nascent (discoidal) high-density lipoproteins (HDL) is essential for formation of matur...

journal_title：Biochemistry

authors： Jones MK,Catte A,Li L,Segrest JP

更新日期：2009-12-01 00:00:00