Characterization of the gamma subunits of the 7S nerve growth factor complex.

abstract：:We prepared a series of cryptogein mutants, an elicitor from Phytophthora cryptogea, with altered abilities to bind sterols and fatty acids. The induction of the early events, i.e., synthesis of active oxygen species and pH changes, in suspension tobacco cells by these mutated proteins was proportional to their abilit...

journal_title：Biochemistry

authors： Lochman J,Kasparovsky T,Damborsky J,Osman H,Marais A,Chaloupkova R,Ponchet M,Blein JP,Mikes V

更新日期：2005-05-03 00:00:00

abstract：:A double mutant cycle has been used to evaluate interaction energies between the global stabilizer mutation asparagine 52 --> isoleucine (N52I) in iso-1-cytochrome c and mutations producing single surface histidines at positions 26, 33, 39, 54, 73, 89, and 100. These histidine mutation sites are distributed through th...

journal_title：Biochemistry

authors： Wandschneider E,Hammack BN,Bowler BE

更新日期：2003-09-16 00:00:00

abstract：:Heparin decreases the rate of inhibition of neutrophil elastase by alpha 1-proteinase inhibitor as a result of its strong binding to the enzyme. Here, we used the slow-binding kinetic approach to decide whether the enzyme-inhibitor interaction proceeds via a two-step mechanism and to identify the step that is affected...

journal_title：Biochemistry

authors： Faller B,Cadène M,Bieth JG

更新日期：1993-09-07 00:00:00

abstract：:Changes in the concentrations of high-energy phosphate metabolites were measured by 31P NMR spectroscopy of surviving rat uteri from 0-48 h following estrogen administration. Concentrations (millimoles per kilogram wet weight) of these metabolites in the untreated immature uterus, measured at 4 degrees C, were found t...

journal_title：Biochemistry

authors： Degani H,Shaer A,Victor TA,Kaye AM

更新日期：1984-06-05 00:00:00

abstract：:The far-ultraviolet circular dichroism spectra of bovine and human prothrombin, prothrombin fragment 1, prethrombin 1, prothrombin fragment 2, and prethrombin 2 (prethrombin 2des(1--13)) were determined and the method of Chen et al. [Chen, Y. H., Yang, J. T., & Martinez, H. M. (1972) Biochemistry 11, 4120--4131; Chen,...

journal_title：Biochemistry

authors： Bloom JW,Mann KG

更新日期：1979-05-15 00:00:00

abstract：:Smooth muscle cells maintain filaments of actin and myosin in the presence of ATP, although dephosphorylated myosin filaments and actin-myosin interactions are unstable under those conditions in vitro. Several proteins that stabilize myosin filaments and that stabilize actin-myosin interactions have been identified. F...

journal_title：Biochemistry

authors： Kingsbury NL,Renegar RH,Chalovich JM

更新日期：2013-10-29 00:00:00

abstract：:Preferential interaction of trans-membrane alpha-helices whose hydrophobic length matches the hydrophobic thickness of the lipid bilayer could be a mechanism of retention in the Golgi apparatus. We have used fluorescence methods to study the interaction of peptides Ac-K2-G-Lm-W-Ln-K2-A-amide (Pm+n) with bilayers of ph...

journal_title：Biochemistry

authors： Webb RJ,East JM,Sharma RP,Lee AG

更新日期：1998-01-13 00:00:00

abstract：:The distribution of one-disulfide bonds in the two-disulfide intermediates in the oxidative refolding of bovine pancreatic ribonuclease A has been characterized. These two-disulfide intermediates were formed from the fully reduced denatured protein by oxidation with dithiothreitol, then blocked with AEMTS, purified by...

journal_title：Biochemistry

authors： Volles MJ,Xu X,Scheraga HA

更新日期：1999-06-01 00:00:00

abstract：:Pili are surface-linked virulence factors that play key roles in infection establishment in a variety of pathogenic species. In Gram-positive pathogens, pilus formation requires the action of sortases, dedicated transpeptidases that covalently associate pilus building blocks. In Streptococcus pneumoniae, a major human...

journal_title：Biochemistry

authors： Manzano C,Izoré T,Job V,Di Guilmi AM,Dessen A

更新日期：2009-11-10 00:00:00

abstract：:Genetic studies have previously demonstrated that the Saccharomyces cerevisiae CDC9 gene product, which is functionally homologous to mammalian DNA ligase I, is required for DNA replication and is also involved in DNA repair and genetic recombination. In the present study we have purified the yeast enzyme. When measur...

journal_title：Biochemistry

authors： Tomkinson AE,Tappe NJ,Friedberg EC

更新日期：1992-12-01 00:00:00

abstract：:We report the use of electrospray ionization (ESI) mass spectrometry (MS) in conjunction with online rapid mixing to monitor the kinetics of acid-induced ferrihemoglobin denaturation. Under equilibrium conditions, the hemoglobin mass spectrum is dominated by the intact heterotetramer. Dimeric and monomeric species are...

journal_title：Biochemistry

authors： Simmons DA,Wilson DJ,Lajoie GA,Doherty-Kirby A,Konermann L

更新日期：2004-11-23 00:00:00

abstract：:Extracts of two human glioma cell lines (lacking O6-methylguanine DNA-methyltransferase) (i.e., A1235 and its alkylation-resistant derivative A1235-MR4) were examined for their ability to execute strand incision at different base mismatches in model (45-bp) DNA. These heteroduplex substrates were of the same sequence ...

journal_title：Biochemistry

authors： Lari SU,Al-Khodairy F,Paterson MC

更新日期：2002-07-23 00:00:00

abstract：:In order to evaluate the contributions that histidine residues might play both in the catalytic activities of endonuclease V and in binding to nontarget DNA, the technique of oligonucleotide site directed mutagenesis was used to create mutations at each of the four histidine residues in the endonuclease V gene. Althou...

journal_title：Biochemistry

authors： Augustine ML,Hamilton RW,Dodson ML,Lloyd RS

更新日期：1991-08-13 00:00:00

abstract：:The structures of the modified folates present in Pyrococcus furiosus have been determined. This was accomplished largely by the characterization of the arylamines resulting from the air oxidative cleavage of the reduced modified folates present in these cells, using both chemical and enzymatic methods. Cell extracts ...

journal_title：Biochemistry

authors： White RH

更新日期：1993-01-26 00:00:00

abstract：:Herpesvirus saimiri codes for a tyrosine kinase interacting protein (Tip) that interacts with both the SH3 domain and the kinase domain of the T-cell-specific tyrosine kinase Lck via two separate motifs. The activation of Lck by Tip is considered as a key event in the transformation of human T-lymphocytes during herpe...

journal_title：Biochemistry

authors： Schweimer K,Hoffmann S,Bauer F,Friedrich U,Kardinal C,Feller SM,Biesinger B,Sticht H

更新日期：2002-04-23 00:00:00

abstract：:Homing endonucleases are distinguished by their ability to catalyze the cleavage of double-stranded DNA with extremely high specificity. I-PpoI endonuclease, a homing endonuclease from the slime mold Physarum polycephalum, is a small enzyme (2 x 20 kDa) of known three-dimensional structure that catalyzes the cleavage ...

journal_title：Biochemistry

authors： Mannino SJ,Jenkins CL,Raines RT

更新日期：1999-12-07 00:00:00

abstract：:The purified ATPase of sarcoplasmic reticulum was covalently labeled with N-iodoacetyl-N'-(5-sulfo-1-naphthyl)ethylenediamine (1,5-IAEDANS) or with iodoacetamidofluorescein (IAF). In reconstituted vesicles containing both types of ATPase molecules fluorescence energy transfer was observed from the IAEDANS (donor) to t...

journal_title：Biochemistry

authors： Vanderkooi JM,Ierokomas A,Nakamura H,Martonosi A

更新日期：1977-04-05 00:00:00

abstract：:The conformation of two fragments derived from anti-poly(D-alanyl) antibodies, the divalent fragment (Fab')2 and the monovalent fragment Fab', was studied by small-angle X-ray scattering before and after interaction with the tetra-D-alanine amide hapten. More than 90% of the combining sites were occupied by the hapten...

journal_title：Biochemistry

authors： Pilz I,Kratky O,Licht A,Sela M

更新日期：1975-03-25 00:00:00

abstract：:The relaxin-like factor (RLF) also named insulin-like 3 (INSL3) consists of two polypeptide chains linked by two interchain and one intrachain disulfide bond. RLF binds to its receptor (LGR8 also named RXFP2) through the B chain and initiates transmembrane communication by activating the adenylate cyclase through the ...

journal_title：Biochemistry

authors： Büllesbach EE,Schwabe C

更新日期：2012-05-22 00:00:00

abstract：:Multiple intracellular receptors of the FK506 binding protein (FKBP) family of peptidylprolyl cis/trans-isomerases are potential targets for the immunosuppressive drug FK506. Inhibition of the protein phosphatase calcineurin (CaN), which has been implicated in the FK506-mediated blockade of T cell proliferation, was s...

journal_title：Biochemistry

authors： Weiwad M,Edlich F,Kilka S,Erdmann F,Jarczowski F,Dorn M,Moutty MC,Fischer G

更新日期：2006-12-26 00:00:00

abstract：:The use of derivatives of alpha-thrombin obtained by limited proteolysis, that have only a single peptide bond cleaved, allowed the unequivocal correlation between the change in covalent structure and alteration of the enzymatic properties. beta T-Thrombin contains a single cleavage in the surface loop corresponding t...

journal_title：Biochemistry

authors： Hofsteenge J,Braun PJ,Stone SR

更新日期：1988-03-22 00:00:00

abstract：:Inducible dimers are powerful tools for controlling biological processes through colocalizing signaling molecules. To be effective, an inducible system should have a dissociation constant in the "off" state that is greater (i.e., weaker affinity) than the concentrations of the molecules that are being controlled, and ...

journal_title：Biochemistry

authors： Zimmerman SP,Hallett RA,Bourke AM,Bear JE,Kennedy MJ,Kuhlman B

更新日期：2016-09-20 00:00:00

abstract：:A neutral protease has been extracted from the media of cultured metastatic tumor cells and purified approximately 1000 times after sequential ammonium sulfate fractionization, concanavalin A column chromatography, and molecular sieve chromatography. The protease has an apparent molecular weight of 70 000--80 000, is ...

journal_title：Biochemistry

authors： Liotta LA,Tryggvason K,Garbisa S,Robey PG,Abe S

更新日期：1981-01-06 00:00:00

abstract：:The binding site of the dopamine D2 receptor, like that of other homologous G protein-coupled receptors, is contained within a water-accessible crevice formed among its seven membrane-spanning segments. Using the substituted-cysteine accessibility method, we previously mapped the residues in the third, fifth, and seve...

journal_title：Biochemistry

authors： Javitch JA,Ballesteros JA,Weinstein H,Chen J

更新日期：1998-01-27 00:00:00

abstract：:Thermal and chemical modification of protein structures is known to affect their interfacial activity. We have looked in detail at the effect of heating on the structure and subsequently the surface properties of LTP1, a nonspecific lipid transfer protein from barley, both in the presence and in the absence of its lip...

journal_title：Biochemistry

authors： Clare Mills EN,Gao C,Wilde PJ,Rigby NM,Wijesinha-Bettoni R,Johnson VE,Smith LJ,Mackie AR

更新日期：2009-12-29 00:00:00

abstract：:Ner repressors of the transposable phages Mu and D108 play a central role in regulating the expression of the early (transposase) operon and in ensuring that phage growth proceeds along a lytic pathway. The latter function is analogous to that performed by the Cro protein of phage lambda. Unlike lambda Cro, however, t...

journal_title：Biochemistry

authors： Benevides JM,Kukolj G,Autexier C,Aubrey KL,DuBow MS,Thomas GJ Jr

更新日期：1994-09-06 00:00:00

abstract：:A novel single polypeptide endopeptidase of 24 kDa (24k-endopeptidase) was purified with a yield of 300-400 microg/L from conditioned medium of a bacterial strain which was identified as a new species in the genus Chryseobacterium Sp. on the basis of its 16S rDNA sequence and DNA:DNA hybridizations. The NH(2)-terminal...

journal_title：Biochemistry

authors： Lijnen HR,Van Hoef B,Ugwu F,Collen D,Roelants I

更新日期：2000-01-18 00:00:00

abstract：:One of the most intriguing questions confronting the bone morphogenetic protein family is the mechanism of ligand recognition, because there are more ligands than receptors. Crystal structures of two type II receptors, ActR-II and BMPR-II, are essentially identical, and a loop structure (A-loop) has been suggested to ...

journal_title：Biochemistry

authors： Yeh LC,Falcon WE,Garces A,Lee JC,Lee JC

更新日期：2012-09-04 00:00:00

abstract：:We have developed a homogeneous time-resolved fluorescence resonance energy transfer (FRET)-based assay that detects the formation of HIV-1 integrase (IN) dimers. The assay utilizes IN monomers that express two different epitope tags that are recognized by their respective antibodies, coupled to distinct fluorophores....

journal_title：Biochemistry

authors： Tsiang M,Jones GS,Hung M,Samuel D,Novikov N,Mukund S,Brendza KM,Niedziela-Majka A,Jin D,Liu X,Mitchell M,Sakowicz R,Geleziunas R

更新日期：2011-03-15 00:00:00

abstract：:The N-terminus of the major polypeptide component of the light-harvesting complex (LHC) from the brown alga Laminaria saccharina is blocked. Two partial sequences, one near the N-terminus and the other near the C-terminus, have been obtained by chemical cleavage with acetic acid and N-chlorosuccinimide. Four peptides ...

journal_title：Biochemistry

authors： Douady D,Rousseau B,Caron L

更新日期：1994-03-22 00:00:00