Abstract：
:The structures of the modified folates present in Pyrococcus furiosus have been determined. This was accomplished largely by the characterization of the arylamines resulting from the air oxidative cleavage of the reduced modified folates present in these cells, using both chemical and enzymatic methods. Cell extracts separated on DEAE-Sephadex columns showed one major peak containing the arylamines derived from the modified folates. These arylamines were not retained on the DEAE-Sephadex columns, indicating that they contained no net negative charge. Purification of the azo dye derivatives of these arylamines on a Bio-Gel P-6 column showed the presence of three different compounds (compounds 1, 2, and 3) in an average amount of 4.1, 7.6, and 22 nmol/g dry weight of cells, respectively. Each of these compounds readily underwent mild acid hydrolysis (0.1 M HCl, 110 degrees C, 1 min) to produce the azo dye derivative of 5-(p-aminophenyl)-1,2,3,4-tetrahydroxypentane (pAPT). The structure and stereochemistry (ribo) of the pAPT was the same as the pAPT present in methanopterin. In addition, compounds 1, 2, and 3 were each shown to contain 1 mol equiv of ribose and 1, 2, and 3 mol equiv of N-acetylglucosamine (gluNAc), respectively, and were designated as the azo dye derivatives of pAPT-ribose-gluNAc, pAPT-ribose-(gluNAc)2, and pAPT-ribose-(gluNAc)3. Each of these compounds was readily cleaved to the azo dye derivative of pAPT-ribose by the enzymatic action of beta-N-acetylglucosaminidase, indicating that all the gluNAc residues were beta-linked.(ABSTRACT TRUNCATED AT 250 WORDS)
journal_name
Biochemistryjournal_title
Biochemistryauthors
White RHdoi
10.1021/bi00054a003subject
Has Abstractpub_date
1993-01-26 00:00:00pages
745-53issue
3eissn
0006-2960issn
1520-4995journal_volume
32pub_type
杂志文章相关文献
BIOCHEMISTRY文献大全abstract：:Propeptide processing occurs in specific compartments of the secretory pathway, but how these processing-competent organelles are generated from their processing-incompetent precursor compartments is unknown. To dissect the process biochemically, we have developed a novel cell-free system reconstituting the production...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi0112762
更新日期：2001-10-30 00:00:00
abstract：:MRP1 (or ABCC1) is an ABC membrane protein that transports a wide range of natural products as well as glutathione (GSH)-, glucuronate-, and sulfate-conjugated metabolites. In addition, free GSH is required for MRP1 to transport several chemotherapeutic drugs. However, the mechanisms regulating the influence of GSH on...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi0268807
更新日期：2003-03-25 00:00:00
abstract：:The promoter fragment -1550/+129 of the human hepatic triglyceride lipase (HTGL) gene drives the expression of the CAT gene in HepG2 cells, albeit at very low levels. Transient transfections in HepG2 and HeLa cells of 5' deletion constructs indicated that the regulatory elements that control this expression are locate...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00088a018
更新日期：1993-09-21 00:00:00
abstract：:Halogen bonding is a weak chemical force that has so far mostly found applications in crystal engineering. Despite its potential for use in drug discovery, as a new molecular tool in the direction of molecular recognition events, it has rarely been assessed in biopolymers. Motivated by this fact, we have developed a p...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/acs.biochem.7b00429
更新日期：2017-06-27 00:00:00
abstract：:Neuroglobin (Ngb) is a recently discovered vertebrate heme protein that is expressed in the brain and can reversibly bind oxygen. Mammalian Ngb is involved in neuroprotection under oxidative stress conditions, such as ischemia and reperfusion. We previously demonstrated that human ferric Ngb binds to the alpha subunit...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi800286m
更新日期：2008-05-13 00:00:00
abstract：:Cytochrome bo from Escherichia coli is a ubiquinol oxidase which is a member of the superfamily of heme-copper respiratory oxidases. This superfamily, which includes the eukaryotic cytochrome c oxidases, has in common a bimetallic center consisting of a high-spin heme component and a copper atom (CuB) which is the sit...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00092a029
更新日期：1993-10-19 00:00:00
abstract：:The transformation of DMPC vesicles containing myristic acid to larger structures was examined by nuclear magnetic resonance techniques. The process was found to be characterized by a rate which is approximately first order in vesicle concentration. Rates, however, show a dramatic increase with increasing myristic aci...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00610a027
更新日期：1978-08-22 00:00:00
abstract：:The nucleotide binding site of the uncoupling protein (UCP) from brown adipose tissue was mapped by photoaffinity labeling with 2-azidoadenosine 5'-triphosphate (2-azido-ATP) and by affinity labeling with 3'-O-(5-fluoro-2,4-dinitrophenyl)adenosine 5'-triphosphate (FDNP-ATP). Both analogs bind with high affinity and sp...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00158a017
更新日期：1992-11-03 00:00:00
abstract：:Fluorescence correlation spectroscopy (FCS) allows the study of interactions of fluorescently labeled ligand with receptors in living cells at single-molecule detection sensitivity. From the autocorrelation functions of fluorescence intensity fluctuations, the diffusion time of molecules through the confocal volume is...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi010514q
更新日期：2001-09-11 00:00:00
abstract：:SH2 domains are protein modules which interact with specific tyrosine phosphorylated sequences in target proteins. The SH2 domain of the Src kinase binds with high affinity to a tyrosine phosphorylated peptide containing the amino acids Glu, Glu, and Ile (EEI) at the positions +1, +2, and +3 C-terminal to the phosphot...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi982974y
更新日期：1999-04-20 00:00:00
abstract：:The oxygen transfer to p-hydroxybenzoate catalyzed by p-hydroxybenzoate hydroxylase (PHBH) has been shown to occur via a C4a-hydroperoxide of the flavin. Two factors are likely to be important in facilitating the transfer of oxygen from the C4a-hydroperoxide to the substrate. (a) The positive electrostatic potential o...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi010892v
更新日期：2001-07-31 00:00:00
abstract：:Much interest is currently focused on understanding the detailed contribution that particular amino acid residues make in protein structure and function. Although the use of site-directed mutagenesis has greatly contributed to this goal, the approach is limited to the standard repertoire of twenty amino acids. Fluorin...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi9617973
更新日期：1997-03-18 00:00:00
abstract：:Tropomyosins from bovine aorta and pulmonary artery exhibit identical electrophoretic patterns in sodium dodecyl sulfate but differ from tropomyosins of either chicken gizzard or rabbit skeletal muscle. Each of the four tropomyosins binds readily to skeletal muscle F-actin as indicated by their sedimentation with acti...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00299a029
更新日期：1984-02-14 00:00:00
abstract：:Diethyl pyrocarbonate (DEP) is an electrophilic reagent that is used to modify reversibly the histidine residues of proteins. Unfortunately, the lability of the acylated histidine adduct usually does not permit the isolation and identification of the modified histidine. By use of 500-MHz proton NMR spectroscopy, it ha...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00445a004
更新日期：1989-09-19 00:00:00
abstract：:The interaction between cytochrome c oxidase complex and adenosine triphosphate synthase (F1F0) complex in the purified, dispersed state and embedded in phospholipid vesicles was studied by differential scanning calorimetry and by spin-label electron paramagnetic resonance. The detergent-dispersed cytochrome oxidase a...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00127a036
更新日期：1992-03-31 00:00:00
abstract：:Two-dimensional mapping of the tryptic phosphopeptides generated following in vitro protein kinase C phosphorylation of the myosin heavy chain isolated from human platelets and chicken intestinal epithelial cells shows a single radioactive peptide. These peptides were found to comigrate, suggesting that they were iden...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00218a012
更新日期：1991-01-29 00:00:00
abstract：:When the single-stranded RNA genome of HIV-1 is copied into double-stranded DNA, the viral enzyme reverse transcriptase (RT) catalyzes the addition of approximately 20,000 nucleotides; however, the precise mechanism of nucleotide addition is unknown. In this study, we attempt to integrate the genetic data and biochemi...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00016a006
更新日期：1995-04-25 00:00:00
abstract：:Calmodulin, the Ca(2+)-dependent activator of many cellular processes, contains two well-defined structural domains, each of which binds two Ca(2+) ions. In its Ca(2+)-free (apo) form, it provides an attractive model for studying mechanisms of protein unfolding, exhibiting two separable, reversible processes, indicati...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi012187s
更新日期：2002-05-28 00:00:00
abstract：:We have found that the atomic force microscope (AFM) can be used to image the "beads-on-a-string" chromatin structure in a normal air environment following adsorption onto a cover glass substrate. Individual nucleosome cores and linker DNA could be resolved clearly along chromatin fibers that were reconstituted using ...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00084a002
更新日期：1993-08-24 00:00:00
abstract：:Recently, a new clathrin assembly protein (AP180) has been purified from coated vesicles of bovine brain (Ahle & Ungewickell, 1986). This protein has been shown to promote polymerization of clathrin into a homogeneous population of baskets under conditions where pure clathrin does not polymerize by itself. We have pur...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00416a040
更新日期：1988-08-09 00:00:00
abstract：:The binding of the R17 coat protein to synthetic RNAs containing one or two coat protein binding sites was characterized by using nitrocellulose filter and gel-retention assays. RNAs with two available sites bound coat protein in a cooperative manner, resulting in a higher affinity and reduced sensitivity to pH, ionic...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00502a006
更新日期：1990-12-18 00:00:00
abstract：:Ferrochelatase is the terminal enzyme in the heme biosynthetic pathway. It catalyzes the insertion of ferrous iron into protoporphyrin IX to produce protoheme IX. The crystal structures of ferrochelatase from Saccharomyces cerevisiae in free form, in complex with Co(II), a substrate metal ion, and in complex with two ...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi0260785
更新日期：2002-11-19 00:00:00
abstract：:Rabbit muscle lactate dehydrogenase (LDH) was coupled to Sepharose in such a way that each molecule is expected to be attached via only one subunit. Dissociation of the bound active enzyme by several methods all yielded immobilized subunit derivatives which were inactive. These derivatives were capable of regenerating...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00664a013
更新日期：1976-09-21 00:00:00
abstract：:Cyclic AMP receptor protein is involved in the regulation of more than 20 genes. A step in the mechanism of activation of transcription is to induce a significant bending of the DNA upon complex formation between specific DNA and the protein. The induced DNA bending and a structure of the protein-DNA complex were stud...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00137a011
更新日期：1992-06-09 00:00:00
abstract：:Type I repeat sequences are evolutionarily conserved sequence elements found in the replication origin and transcriptional promoter region of the rRNA genes (rDNA) in Tetrahymena thermophila. An abundant single-stranded DNA binding protein, ssA-TIBF, specifically interacts with the A-rich strand of the Type I repeat s...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00014a011
更新日期：1995-04-11 00:00:00
abstract：:N1-(5'-Phosphoribosyl)adenosine-5'-monophosphate cyclohydrolase (HisI, PR-AMP cyclohydrolase) is a central enzyme in histidine biosynthesis catalyzing the hydrolysis of the N1-C6 bond of the purine substrate, a reaction unique to this pathway. A source of the recombinant monofunctional Methanococcus vannielii PR-AMP c...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi982475x
更新日期：1999-02-02 00:00:00
abstract：:The mechanism of binding of reduced 1-(2-carboxyphenylamino)-1-deoxyribulose 5-phosphate (rCdRP) to two different binding sites on the bifunctional enzyme is determined by kinetic studies, using temperature-jump and stopped-flow equipment with fluorescence detection. Two rapid binding processes and a comparatively slo...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00593a030
更新日期：1979-12-25 00:00:00
abstract：:Prolyl endopeptidase (Prep) is a member of the prolyl peptidase family and is of interest because of its unique biochemistry and connections to cognitive function. Using an unbiased mass spectrometry (MS)-based peptidomics platform, we identified Prep-regulated peptides in the central nervous system (CNS) of mice by m...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi901637c
更新日期：2009-12-22 00:00:00
abstract：:The Sir2 enzyme family is responsible for a newly classified chemical reaction, NAD(+)-dependent protein deacetylation. New peptide substrates, the reaction mechanism, and the products of the acetyl transfer to NAD(+) are described for SIR2. The final products of SIR2 reactions are the deacetylated peptide and the 2' ...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi011858j
更新日期：2001-12-25 00:00:00
abstract：:The reaction of Pseudomonas fluorescens kynureninase with L-kynurenine and L-alanine has been examined using rapid-scanning stopped-flow spectrophotometry. Mixing kynureninase with 0.5 mM L-kynurenine results in formation of a quinonoid intermediate, with lambdamax = 494 nm, within the dead time (ca. 2 ms) of the stop...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi980066v
更新日期：1998-06-16 00:00:00
