Calorimetric examination of high-affinity Src SH2 domain-tyrosyl phosphopeptide binding: dissection of the phosphopeptide sequence specificity and coupling energetics.

abstract：:The orphan receptor short heterodimer partner (SHP) is a common partner for a great number of nuclear receptors, and it plays an important role in many diverse physiological events. In a previous study, we described SHP as a strong repressor of the androgen receptor (AR). Herein, we addressed the mechanism of action o...

journal_title：Biochemistry

authors： Gobinet J,Carascossa S,Cavaillès V,Vignon F,Nicolas JC,Jalaguier S

更新日期：2005-04-26 00:00:00

abstract：:Among hormone-inducible transcription factors, estrogen receptors (ERs) play important roles in tissue growth and differentiation, via either direct or indirect binding, in the nucleus, to specific DNA targets called estrogen responsive elements (EREs), or through nongenomic pathways. In humans, two estrogen receptor ...

journal_title：Biochemistry

authors： Le Grand A,André-Leroux G,Marteil G,Duval H,Sire O,Le Tilly V

更新日期：2015-06-30 00:00:00

abstract：:Factor VIII (FVIII) is the nonproteolytic cofactor for FIXa in the tenase complex of blood coagulation. FVIII is proteolytically activated by thrombin and FXa in vitro to form a heterotrimer with full procoagulant activity. Activated protein C inactivates thrombin-activated FVIII through cleavage adjacent to position ...

journal_title：Biochemistry

authors： O'Brien DP,Johnson D,Byfield P,Tuddenham EG

更新日期：1992-03-17 00:00:00

abstract：:Flavohemoglobins (flavoHbs) are enzymes that operate primarily as nitric oxide dioxygenases and shuttle thereby electrons among NAD(P)H, FAD, heme, and a ligated redox-active substrate such as O(2). They function in the bacterial defense against nitrosative stress and are therefore considered as targets for new antibi...

journal_title：Biochemistry

authors： El Hammi E,Warkentin E,Demmer U,Limam F,Marzouki NM,Ermler U,Baciou L

更新日期：2011-02-22 00:00:00

abstract：:Pepstatin is a slow and tight-binding inhibitor of pepsin. Preincubating enzyme and inhibitor in H2O and in D2O in the absence of substrate generates an inverse solvent isotope effect of Dk = 0.69 +/- 0.06 on the apparent first-order rate constant for the decay in enzymatic activity. Proton inventory analysis of the i...

journal_title：Biochemistry

authors： Cho YK,Rebholz KL,Northrop DB

更新日期：1994-08-16 00:00:00

abstract：:The Iowa point mutation in apolipoprotein A-I (G26R) leads to a systemic amyloidosis condition, and the Milano mutation (R173C) is associated with hypoalphalipoproteinemia, a reduced plasma level of high-density lipoprotein. To probe the structural effects that lead to these outcomes, we used amide hydrogen-deuterium ...

journal_title：Biochemistry

authors： Chetty PS,Ohshiro M,Saito H,Dhanasekaran P,Lund-Katz S,Mayne L,Englander W,Phillips MC

更新日期：2012-11-06 00:00:00

abstract：:The mature form of subtilisin is an unusual example of a monomeric protein with a high kinetic barrier to folding and unfolding. Using site-directed mutagenesis of subtilisin BPN', we are attempting to determine the physical and energetic nature of the kinetic barrier. The high-affinity calcium-binding site A has been...

journal_title：Biochemistry

authors： Strausberg S,Alexander P,Wang L,Gallagher T,Gilliland G,Bryan P

更新日期：1993-10-05 00:00:00

abstract：:The existence of temperature optima in enzyme catalysis that occur before protein melting sets in can be described by different types of kinetic models. Such optima cause distinctly curved Arrhenius plots and have, for example, been observed in several cold-adapted enzymes from psychrophilic species. The two main expl...

journal_title：Biochemistry

authors： Åqvist J,Sočan J,Purg M

更新日期：2020-10-13 00:00:00

abstract：:The photoaddition of 4'-(hydroxymethyl)-4,5',8-trimethylpsoralen (HMT) to different conformational states of RNA was studied. Poly(U), poly(A,U) (random copolymer), poly(A-U) (alternating copolymer), poly(A) . poly(U) (double stranded), and poly(U) . poly(A) . poly(U) (triple stranded) were reacted with HMT at differe...

journal_title：Biochemistry

authors： Thompson JF,Bachellerie JP,Hall K,Hearst JE

更新日期：1982-03-16 00:00:00

abstract：:Prion diseases are associated with the conversion of the normal prion protein, PrP(C), to the infectious disease form PrP(Sc). Discrimination between these isoforms would significantly enhance diagnosis of these diseases, and it has recently been reported that PrP(Sc) is specifically recognized by the serine protease ...

journal_title：Biochemistry

authors： Ellis V,Daniels M,Misra R,Brown DR

更新日期：2002-06-04 00:00:00

abstract：:4-Oxalocrotonate tautomerase (EC 5.3.2-; 4-OT), a hexamer consisting of 62 residues per subunit, catalyzes the isomerization of unsaturated alpha-keto acids, converting unconjugated ketones to the conjugated isomers via a dienolic intermediate. The recently solved crystal structure of an isozyme of 4-OT suggests that ...

journal_title：Biochemistry

authors： Stivers JT,Abeygunawardana C,Mildvan AS,Hajipour G,Whitman CP,Chen LH

更新日期：1996-01-23 00:00:00

abstract：:The aggregation of the microtubule-associated protein, tau, into amyloid fibrils is a hallmark of neurodegenerative diseases such as the tauopathies and Alzheimer's disease. Since monomeric tau is an intrinsically disordered protein and the polymeric fibrils possess an ordered cross-β core, the aggregation process is ...

journal_title：Biochemistry

authors： Ramachandran G,Milán-Garcés EA,Udgaonkar JB,Puranik M

更新日期：2014-10-21 00:00:00

abstract：:Hypochlorous acid (HOCl) is a powerful oxidant generated from H(2)O(2) and Cl(-) by the heme enzyme myeloperoxidase, which is released from activated leukocytes. HOCl possesses potent antibacterial properties, but excessive production can lead to host tissue damage that is implicated in a wide range of human diseases ...

journal_title：Biochemistry

authors： Pattison DI,Davies MJ

更新日期：2006-07-04 00:00:00

abstract：:The amidotransferase family of enzymes utilizes the ammonia derived from the hydrolysis of glutamine for a subsequent chemical reaction catalyzed by the same enzyme. The ammonia intermediate does not dissociate into solution during the chemical transformations. A well-characterized example of the structure and mechani...

journal_title：Biochemistry

authors： Raushel FM,Thoden JB,Holden HM

更新日期：1999-06-22 00:00:00

abstract：:The effect of opportunistic infections (OI) on immune-compromised populations has been known for decades, but the recent AIDS epidemic has sparked renewed interest in the development of new anti-OI agents. The mechanism of action of a series of cationic unfused-aromatic anti-OI drugs is believed to involve binding of ...

journal_title：Biochemistry

authors： Tanious FA,Ding D,Patrick DA,Tidwell RR,Wilson WD

更新日期：1997-12-09 00:00:00

abstract：:Differential expression of subunit genes from the nicotinic acetylcholine receptor (AChR) superfamily yields distinct receptor subtypes. As each AChR subtype has a specific subunit composition and many subunit combinations appear not to be expressed, each subunit must contain some information leading to proper assembl...

journal_title：Biochemistry

authors： García-Guzmán M,Sala F,Sala S,Campos-Caro A,Criado M

更新日期：1994-12-20 00:00:00

abstract：:The three complete human LDL receptor homology regions of the LDL receptor-related protein (sLRP2, sLRP3, and sLRP4) have been expressed in Pichia pastoris SMD1168 with constitutive coexpression of the receptor-associated protein (RAP). Each sLRP was purified to homogeneity after deglycosylation using a combination of...

journal_title：Biochemistry

authors： Croy JE,Shin WD,Knauer MF,Knauer DJ,Komives EA

更新日期：2003-11-11 00:00:00

abstract：:In aqueous glycerol solutions, the rate constant for the formation of compound I of horseradish peroxidase from hydrogen peroxide is independent of the viscosity. However, whem m-chloroperbenzoic acid is used to form compound I, the rate is viscosity dependent, indicating that this reaction is diffusion controlled. Th...

journal_title：Biochemistry

authors： Dunford HB,Hewson WD

更新日期：1977-06-28 00:00:00

abstract：:Recent studies of proteins with reversed charged residues have demonstrated that electrostatic interactions on the surface can contribute significantly to protein stability. We have used the approach of reversing negatively charged residues using Arg to evaluate the effect of the electrostatics context on the transiti...

journal_title：Biochemistry

authors： Mancusso R,Cruz E,Cataldi M,Mendoza C,Fuchs J,Wang H,Yang X,Tasayco ML

更新日期：2004-04-06 00:00:00

abstract：:Peptides and peptide-like molecules as a class have very poor permeability through biological membranes, which severely compromises their potential effectiveness as therapeutic agents. In order to gain insight into the problem of delivering peptide and protein drugs and to establish a model in which the effects of sys...

journal_title：Biochemistry

authors： Gray RA,Vander Velde DG,Burke CJ,Manning MC,Middaugh CR,Borchardt RT

更新日期：1994-02-15 00:00:00

abstract：:The interaction between the beta 2 subunit of tryptophan synthase and the coenzyme pyridoxal 5'-phosphate (PLP) is characterized by induced circular dichroism (CD) in the near-UV (260-285 nm) and in the visible region (320-480 nm, extrinsic Cotton effect). Because of its high mean residue ellipticity ([theta] = 56 deg...

journal_title：Biochemistry

authors： Balk H,Merkl I,Bartholmes P

更新日期：1981-10-27 00:00:00

abstract：:Calcium trapped within sonicated and resealed bovine rod outer segment disks is released upon light exposure with a stoichiometry of 0.75 +/- 0.05 calcium for each rhodopsin bleached. The amount of calcium liberated is proportional to the amount of bleaching in the range of 20 to 100% bleaching and is relatively insen...

journal_title：Biochemistry

authors： Smith HG Jr,Fager RS,Litman RJ

更新日期：1977-04-05 00:00:00

abstract：:Heme oxygenase (HO) catalyzes the regiospecific cleavage of the porphyrin ring of heme using reducing equivalents and O2 to produce biliverdin, iron, and CO. Because CO has a cytoprotective effect through the p38-MAPK pathway, HO is a potential therapeutic target in cancer. In fact, inhibition of the HO isoform HO-1 r...

journal_title：Biochemistry

authors： Sugishima M,Higashimoto Y,Oishi T,Takahashi H,Sakamoto H,Noguchi M,Fukuyama K

更新日期：2007-02-20 00:00:00

abstract：:Galactolipids are characteristic lipids of the photosynthetic membranes. They are highly enriched in the chloroplast and are present in photosystem structures. There are two major types of galactolipids, i.e., monogalactosyldiacylglycerol and digalactosyldiacylglycerol (DGDG) in chloroplastic membranes, which amount t...

journal_title：Biochemistry

authors： Fu B,Brown C,Mäler L

更新日期：2020-03-03 00:00:00

abstract：:An overexpression system for spinach apocytochrome b(6) as a fusion protein to a maltose-binding protein in Escherichia coli was established using the expression vector pMalp2. The fusion of the cytochrome b(6) to the periplasmic maltose-binding protein directs the cytochrome on the Sec-dependent pathway. The cytochro...

journal_title：Biochemistry

authors： Króliczewski J,Hombek-Urban K,Szczepaniak A

更新日期：2005-05-24 00:00:00

abstract：:The fatty acid synthase (FAS) of animal tissue is a dimer of two identical subunits, each with a Mr of 260,000. The subunit is a single multifunctional protein having seven catalytic activities and a site for binding of the prosthetic group 4'-phosphopantetheine. The mRNA coding for the subunit has an estimated size o...

journal_title：Biochemistry

authors： Kasturi R,Chirala S,Pazirandeh M,Wakil SJ

更新日期：1988-10-04 00:00:00

abstract：:Treatment of murine leukemia virus reverse transcriptase (MuLV RT) with potassium ferrate, an oxidizing agent known to oxidize amino acids involved in phosphate binding domains of proteins, results in the irreversible inactivation of both the DNA polymerase and the RNase H activities. Significant protection from ferra...

journal_title：Biochemistry

authors： Reddy G,Nanduri VB,Basu A,Modak MJ

更新日期：1991-08-20 00:00:00

abstract：:The effects of an inhibitory monoclonal antibody (mAb) raised against the beta subunit of the Escherichia coli RNA polymerase were determined on the kinetics and structural interactions during formation of the open promoter complex (RPo). Analysis of the kinetics of abortive initiation on linear and supercoiled templa...

journal_title：Biochemistry

authors： Rockwell P,Krakow JS

更新日期：1988-05-03 00:00:00

abstract：:Two glutathione S-transferase (GST) activities have been identified and purified from etiolated corn tissue. The first, designated GST I enzyme, is constitutively present in corn tissue, and the second, designated GST II enzyme, is present only in tissue which has been treated with chemical antidotes which protect cor...

journal_title：Biochemistry

authors： Mozer TJ,Tiemeier DC,Jaworski EG

更新日期：1983-03-01 00:00:00

abstract：:Limited cleavages of human C1r by extrinsic proteases of various specificity (plasmin, elastase, chymotrypsin, thermolysin) yield dimeric associations of two globular domains, each comprised of the intact B chain disulfide linked to gamma, the C-terminal fragment of the A chain. These (gamma-B)2 domains, which are hom...

journal_title：Biochemistry

authors： Arlaud GJ,Gagnon J,Villiers CL,Colomb MG

更新日期：1986-09-09 00:00:00