Identification of a cis-acting negative DNA element which modulates human hepatic triglyceride lipase gene expression.

abstract：:Amyloid fibrils comprising primarily the peptides A beta 40 and A beta 42 are a defining feature of the Alzheimer's disease (AD) brain, and convergent evidence suggests that the process of their formation plays a central role in the AD pathogenic pathway. Elucidation of fibril assembly is critical for the discovery of...

journal_title：Biochemistry

authors： Harper JD,Wong SS,Lieber CM,Lansbury PT Jr

更新日期：1999-07-13 00:00:00

abstract：:1,4-Dihydroxy-2-naphthoyl-coenzyme A (DHNA-CoA) synthase, or MenB, catalyzes an intramolecular Claisen condensation involving two oxyanion intermediates in the biosynthetic pathway of menaquinone, an essential respiration electron transporter in many microorganisms. Here we report the finding that the DHNA-CoA product...

journal_title：Biochemistry

authors： Chen M,Jiang M,Sun Y,Guo ZF,Guo Z

更新日期：2011-07-05 00:00:00

abstract：:Control of protein phosphatases is now understood to depend on binding to a variety of regulatory or targeting subunits to form holoenzymes with restricted localization and substrate specificity. In addition, the catalytic subunits of both type-1 and type-2 phosphatases bind specific inhibitor proteins. Here, we repor...

journal_title：Biochemistry

authors： Eto M,Karginov A,Brautigan DL

更新日期：1999-12-21 00:00:00

abstract：:Voltage-dependent L-type calcium (Ca) channels are heteromultimeric proteins that are regulated through phosphorylation by cAMP-dependent protein kinase (PKA). We demonstrated that the beta 2 subunit was a substrate for PKA in intact cardiac myocytes through back-phosphorylation experiments. In addition, a heterologou...

journal_title：Biochemistry

authors： Gerhardstein BL,Puri TS,Chien AJ,Hosey MM

更新日期：1999-08-10 00:00:00

abstract：:Several fusions between the gene for human insulin-like growth factor I (IGF-I) and the genes for different IgG-binding fragments of staphylococcal protein A were assembled and compared regarding expression, secretion, and purification of the peptide hormone. After IgG affinity purification of the fusion proteins from...

journal_title：Biochemistry

authors： Moks T,Abrahmsén L,Holmgren E,Bilich M,Olsson A,Uhlén M,Pohl G,Sterky C,Hultberg H,Josephson S

更新日期：1987-08-25 00:00:00

abstract：:NADPH-cytochrome P450 oxidoreductase (P450 reductase, EC 1.6.2.4) is an essential component of the P450 monooxygenase complex and binds FMN, FAD, and NADPH cofactors. Residues Tyr140 and Tyr178 are known to be involved in FMN binding. A third aromatic side chain, Phe181, is also located in the proximity of the FMN rin...

journal_title：Biochemistry

authors： Paine MJ,Ayivor S,Munro A,Tsan P,Lian LY,Roberts GC,Wolf CR

更新日期：2001-11-13 00:00:00

abstract：:Oxygen and nitroaromatic compounds are known to enhance the sensitivity of cells to ionizing radiation. Employing calf thymus DNA and oligo(dA)12/poly(dT), we have examined the differences to DNA damage, in particular thymine glycols and the 3'-DNA termini at strand breaks, arising from irradiation under anoxic and ox...

journal_title：Biochemistry

authors： Buchko GW,Weinfeld M

更新日期：1993-03-09 00:00:00

abstract：:The thermal denaturation of caricain (the most alkaline of papain-related proteinases) was studied in acid media. Under all conditions tested, caricain denatured irreversibly following a single first-order reaction that involves simultaneous loss of secondary and tertiary structures. Besides, variation of the rate con...

journal_title：Biochemistry

authors： López-Arenas L,Solís-Mendiola S,Hernández-Arana A

更新日期：1999-11-30 00:00:00

abstract：:RNA helicase DHX33 has been shown to participate in a variety of cellular activities, including ribosome biogenesis, protein translation, and gene transcription. We and others further discovered that DHX33 is strongly expressed in several types of human cancers and plays important roles in promoting cancer cell prolif...

journal_title：Biochemistry

authors： Wang X,Ge W,Zhang Y

更新日期：2019-01-29 00:00:00

abstract：:In iron-deficient conditions, Azomonas macrocytogenes ATCC 12334 excretes a fluorescent siderophore called azoverdin, which is composed of a six-amino-acid peptide chain linked to a chromophore. Azoverdin chelates iron(III) very strongly, solubilizing it and transporting it back into the cells using an outer-membrane ...

journal_title：Biochemistry

authors： Wasielewski E,Atkinson RA,Abdallah MA,Kieffer B

更新日期：2002-10-15 00:00:00

abstract：:The binding of agonists, antagonists, and the acetylcholinesterase inhibitor, eserine, to the nicotinic acetylcholine receptor from Torpedo californica has been monitored by the fluorescence changes of two extrinsic probes that have been covalently attached to the receptor protein. Although both probes, 5-(iodoacetami...

journal_title：Biochemistry

authors： Dunn SM,Raftery MA

更新日期：1993-08-24 00:00:00

abstract：:The environmentally sensitive fluorescent probes 6-propionyl-2-(N,N-dimethylamino)naphthalene (PRODAN) and 2'-(N,N-dimethylamino)-6-naphthoyl-4-trans-cyclohexanioc acid (DANCA) form complexes with the heme binding site of apohorseradish peroxidase. The dissociation constants of the PRODAN and DANCA complexes were dete...

journal_title：Biochemistry

authors： Lasagna M,Vargas V,Jameson DM,Brunet JE

更新日期：1996-01-23 00:00:00

abstract：:FixL is a sensor histidine kinase having a heme-containing domain as an O(2) sensing site. In the study presented here, Ile209 and Ile210 located near the heme iron of the heme domain of Rhizobium meliloti FixL (RmFixL) were mutated, and the mutational effects on the regulation of the kinase activity and the heme pock...

journal_title：Biochemistry

authors： Mukai M,Nakamura K,Nakamura H,Iizuka T,Shiro Y

更新日期：2000-11-14 00:00:00

abstract：:Gram-negative bacteria, such as Escherichia coli, utilize efflux resistance systems in order to expel toxins from their cells. Heavy-metal resistance is mediated by resistance nodulation cell division (RND)-based efflux pumps composed of a tripartite complex that includes an RND-transporter, an outer-membrane factor (...

journal_title：Biochemistry

authors： Mealman TD,Zhou M,Affandi T,Chacón KN,Aranguren ME,Blackburn NJ,Wysocki VH,McEvoy MM

更新日期：2012-08-28 00:00:00

abstract：:The ligand-binding dynamics and the reaction with O(2) of the fully (five-electron) reduced cytochrome caa(3) from the thermohalophilic bacterium Rhodothermus (R.) marinus were investigated. The enzyme is a proton pump which has all the residues of the proton-transfer pathways found in the mitochondrial-like enzymes c...

journal_title：Biochemistry

authors： Sigurdson H,Namslauer A,Pereira MM,Teixeira M,Brzezinski P

更新日期：2001-09-04 00:00:00

abstract：:Human immunodeficiency virus type 1 (HIV-1) Vpu is an integral membrane protein that belongs to the viroporin family. Viroporins interact with cell membranes, triggering membrane permeabilization and promoting release of viral particles. In vitro electrophysiological methods have revealed changes in membrane ion curre...

journal_title：Biochemistry

authors： Herrero L,Monroy N,González ME

更新日期：2013-01-08 00:00:00

abstract：:Guanylin, an intestinal peptide hormone and endogenous ligand of guanylyl cyclase C, is produced as the corresponding prohormone proguanylin. The mature hormone consists of 15 amino acid residues, representing the COOH-terminal part of the prohormone comprised of 94 amino acid residues. Here we report the recombinant ...

journal_title：Biochemistry

authors： Lauber T,Nourse A,Schulz A,Marx UC

更新日期：2002-12-10 00:00:00

abstract：:Several different monoclonal antibodies (mAbs) have been actively developed in the field of Alzheimer's disease (AD) for basic science and clinical applications; however, the binding kinetics of many of the mAbs with the beta-amyloid peptides (Abeta) are poorly understood. A panel of mAbs with different Abeta recognit...

journal_title：Biochemistry

authors： Ramakrishnan M,Kandimalla KK,Wengenack TM,Howell KG,Poduslo JF

更新日期：2009-11-03 00:00:00

abstract：:NADP(H) binding is essential for fast electron transfer through the flavoprotein domain of the fusion protein P450BM3. Here we characterize the interaction of NADP(H) with the oxidized and partially reduced enzyme and the effect of this interaction on the redox properties of flavin cofactors and electron transfer. Mea...

journal_title：Biochemistry

authors： Murataliev MB,Feyereisen R

更新日期：2000-10-17 00:00:00

abstract：:Apolipoprotein A-I (apoA-I) is the main protein of plasma high-density lipoproteins (HDL, or good cholesterol) that remove excess cell cholesterol and protect against atherosclerosis. In hereditary amyloidosis, mutations in apoA-I promote its proteolysis and the deposition of the 9-11 kDa N-terminal fragments as fibri...

journal_title：Biochemistry

authors： Gursky O,Mei X,Atkinson D

更新日期：2012-01-10 00:00:00

abstract：:Phospholipase A2 from Naja naja kaouthia venom was covalently coupled onto agarose beads using two different chemistries. The effect of micellar competitive inhibitors in the coupling media was evaluated. Enzyme bound to N-hydroxysuccinimide-activated agarose, which is reactive primarily toward epsilon-amino groups, h...

journal_title：Biochemistry

authors： Ferreira JP,Sasisekharan R,Louie O,Langer R

更新日期：1993-08-17 00:00:00

abstract：:Proteins have many distinct tertiary folds (Richardson, J. S. (1981) Adv. Prot. Chem. 34, 167-339). The term tertiary fold refers to the spatial organization of secondary structure elements (alpha-helices and beta-strands). It is not known when, in the process of protein folding, a native tertiary fold emerges. Here, ...

journal_title：Biochemistry

authors： Peng ZY,Kim PS

更新日期：1994-03-01 00:00:00

abstract：:The zinc-dependent metallo-beta-lactamases are a group of bacterial enzymes that pose a threat to the future efficacy of present-day antibiotics. Their mechanism is poorly understood, and there are no clinically useful inhibitors. While most members of the group contain two tightly bound zinc ions in their active site...

journal_title：Biochemistry

authors： Davies AM,Rasia RM,Vila AJ,Sutton BJ,Fabiane SM

更新日期：2005-03-29 00:00:00

abstract：:An assay was developed to study the spontaneous transfer and transbilayer movement (flip-flop) of lipid analogs labeled with the fluorescent fatty acid, 5-(5,7-dimethyl BODIPY)-1-pentanoic acid (C5-DMB-) in large unilamellar lipid vesicles comprised of 1-palmitoyl-2-oleoyl phosphatidylcholine (POPC). The assay is base...

journal_title：Biochemistry

authors： Bai J,Pagano RE

更新日期：1997-07-22 00:00:00

abstract：:Ca2+-activated Z-disk-removing activity in the P0-40 crude muscle extracts described by Busch et al. (Busch, W. A., Stromer, M. H., Goll, D. E., and Suzuki, A. (1972), J. Cell Biol. 52, 367) was purified from porcine skeletal muscle extracts by using five column chromatographic procedures in succession: (1) 6% agarose...

journal_title：Biochemistry

authors： Dayton WR,Goll DE,Zeece MG,Robson RM,Reville WJ

更新日期：1976-05-18 00:00:00

abstract：:We have determined the nucleotide sequence and the derived amino acid sequence of the phosphoprotein-encoding region of the chicken vitellogenin III gene. The sequence of this minor vitellogenin could be aligned with exon 22 up to exon 27 of the previously sequenced major vitellogenin II gene (van het Schip et al., 19...

journal_title：Biochemistry

authors： Byrne BM,de Jong H,Fouchier RA,Williams DL,Gruber M,Ab G

更新日期：1989-03-21 00:00:00

abstract：:Weak or nonexistent smallpox immunity in today's human population raises concerns about the possibility of natural or provoked genetic modifications leading to re-emergence of variola virus and other poxviruses. Thus, the development of new antiviral strategies aimed at poxvirus infections in humans is a high priority...

journal_title：Biochemistry

authors： Duraffour S,Ishchenko AA,Saparbaev M,Crance JM,Garin D

更新日期：2007-10-23 00:00:00

abstract：:Two glutathione S-transferase (GST) activities have been identified and purified from etiolated corn tissue. The first, designated GST I enzyme, is constitutively present in corn tissue, and the second, designated GST II enzyme, is present only in tissue which has been treated with chemical antidotes which protect cor...

journal_title：Biochemistry

authors： Mozer TJ,Tiemeier DC,Jaworski EG

更新日期：1983-03-01 00:00:00

abstract：:Cystathionine β-synthase (CBS) catalyzes the first step in the transsulfuration pathway in mammals, i.e., the condensation of serine and homocysteine to produce cystathionine and water. Recently, we have reported a steady-state kinetic analysis of the three hydrogen sulfide (H(2)S)-generating reactions that are cataly...

journal_title：Biochemistry

authors： Singh S,Ballou DP,Banerjee R

更新日期：2011-01-25 00:00:00

abstract：:Enolase is a glycolytic metalloenzyme involved in carbon metabolism. The advantage of targeting enolase lies in its essentiality in many biological processes such as cell wall formation and RNA turnover and as a plasminogen receptor. We initially used a DARTS assay to identify enolase as a target in Escherichia coli. ...

journal_title：Biochemistry

authors： Krucinska J,Falcone E,Erlandsen H,Hazeen A,Lombardo MN,Estrada A,Robinson VL,Anderson AC,Wright DL

更新日期：2019-03-05 00:00:00