Abstract:
:Guanylin, an intestinal peptide hormone and endogenous ligand of guanylyl cyclase C, is produced as the corresponding prohormone proguanylin. The mature hormone consists of 15 amino acid residues, representing the COOH-terminal part of the prohormone comprised of 94 amino acid residues. Here we report the recombinant expression and purification of proguanylin with its native disulfide connectivity, as well as the biophysical characterization of the recombinant and native protein. The comparison of recombinant and native proguanylin revealed identical biophysical and structural properties, as deduced from CZE, HPLC, and mass spectrometry, as well as NMR spectroscopy and CD spectroscopy at various temperatures and pH values. Exhaustive analytical ultracentrifugation studies were employed for protein concentrations up to the millimolar range to determine the association state of recombinant as well as native proguanylin, revealing both proteins to be monomeric at the applied solution conditions. As a result, a former identified close proximity between the termini of proguanylin is due to intramolecular interactions.
journal_name
Biochemistryjournal_title
Biochemistryauthors
Lauber T,Nourse A,Schulz A,Marx UCdoi
10.1021/bi026434jsubject
Has Abstractpub_date
2002-12-10 00:00:00pages
14602-12issue
49eissn
0006-2960issn
1520-4995pii
bi026434jjournal_volume
41pub_type
杂志文章相关文献
BIOCHEMISTRY文献大全abstract::Discoidal high-density lipoprotein (HDL) particles are known to be fractionalized into several discrete populations in plasma and to differ in behavior according to size; however, their structural differences and the factors regulating their size are less understood. In this study, we prepared several reconstituted HD...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi900785x
更新日期:2009-08-18 00:00:00
abstract::Spectroscopic characterization of the newly discovered heme-PAS domain sensor protein BxRcoM-2 reveals that this protein undergoes redox-dependent ligand switching and CO- and NO-induced ligand displacement. The aerobic bacterium Burkholderia xenovorans expresses two homologous heme-containing proteins that promote CO...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi800486x
更新日期:2008-08-26 00:00:00
abstract::The EphA2 receptor plays key roles in many physiological and pathological events, including cancer. The process of receptor endocytosis and the consequent degradation have attracted attention as possible means of overcoming the negative outcomes of EphA2 in cancer cells and decreasing tumor malignancy. A recent study ...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi300141h
更新日期:2012-03-13 00:00:00
abstract::Membranes derived from free floating granulosa cells in porcine ovarian follicular fluid were used as a starting material for structural characterization of both LH/hCG and FSH receptors. The receptors were highly hormone-specific and showed single classes of high-affinity binding sites (Kd = 19-74 pM). Their molecula...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00467a022
更新日期:1990-04-17 00:00:00
abstract::The present study was undertaken for the purpose of evaluating the solvent perturbation technique as a method to identify enzyme catalytic residues. For establishment of expected directions and sizes of pKa perturbations for different types of acids in different classes of solvents, a study of the pKa of a series of a...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00287a013
更新日期:1983-08-30 00:00:00
abstract::Blood coagulation is initiated by Ca(2+)-dependent binding of coagulation factor VIIa (FVIIa) to its cofactor, tissue factor (TF). The TF:FVIIa complex activates factors IX and X, ultimately leading to the formation of thrombin and the coagulation of blood. FVII consists of an N-terminal gamma-carboxyglutamic-acid-con...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi980522f
更新日期:1998-07-28 00:00:00
abstract::The two key structural features of alpha 2-macroglobulin (alpha 2M) involved in inhibitory caging of proteases are the thiol ester and the bait region. This paper examines the environment of the hydrolyzed thiol ester in methylamine-treated human alpha 2M and the separation between the bait region and the thiol ester ...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00408a036
更新日期:1988-04-19 00:00:00
abstract::Structurally diverse small molecules, including 5-(2-benzofuryl)-4-phenyl-1,2,4-triazole-3-thiol (BETT), have been identified via high-throughput screening as activators of caspase-3 in HeLa cell extracts. However, little is known about their mechanism of action. In this study, we investigate how BETT regulates prothy...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi9022329
更新日期:2010-03-09 00:00:00
abstract::A new orally active oxidosqualene:lanosterol cyclase (OSLC) inhibitor (Ro48-8071; Morand, O. H. et al. (1997) J. Lipid Res. 38, 373-390) showed potent noncompetitive inhibition of bacterial squalene:hopene cyclase (SHC) from Alicyclobacillus acidocaldarius (IC50 = 9.0 nM, KI = 6.6 nM) and OSLC (IC50 = 40 nM, KI = 22 n...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi980366c
更新日期:1998-04-28 00:00:00
abstract::Smooth muscle cells maintain filaments of actin and myosin in the presence of ATP, although dephosphorylated myosin filaments and actin-myosin interactions are unstable under those conditions in vitro. Several proteins that stabilize myosin filaments and that stabilize actin-myosin interactions have been identified. F...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi401013g
更新日期:2013-10-29 00:00:00
abstract::The sensor kinase CitA and the response regulator CitB of Klebsiella pneumoniae form the paradigm of a subfamily of bacterial two-component regulatory systems that are capable of sensing tri- or dicarboxylates in the environment and then induce transporters for the uptake of these compounds. We recently showed that th...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi0340595
更新日期:2003-05-20 00:00:00
abstract::Formylglycinamide ribonucleotide amidotransferase (FGAR-AT) catalyzes the ATP- and glutamine-dependent formation of formylglycinamidine ribonucleotide, ADP, P(i), and glutamate in the fourth step of de novo purine biosynthesis. Like all amidotransferases (ATs), FGAR-AT is proposed to channel ammonia between a glutamin...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi049127h
更新日期:2004-08-17 00:00:00
abstract::In the HLH-LZ protein family, the helix-loop-helix DNA-binding dimerization domain is followed in the sequence by a leucine zipper motif. The precise function of this second dimerization domain is still unclear, since the HLH motif of a subset of this family has been shown to be necessary and sufficient for dimerizati...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00203a027
更新日期:1994-09-20 00:00:00
abstract::Drug efflux and enzymatic drug degradation are two cellular mechanisms that contribute to drug resistance in many cancers. Herein, we report the synthesis and in vitro activity of a pro-immunostimulant that exploits both processes in tandem to selectively confer cancer-mediated immunogenicity. We demonstrate that an i...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/acs.biochem.8b00095
更新日期:2018-04-17 00:00:00
abstract::Hydrophobic interactions are responsible for stabilizing leucine zippers in peptides containing heptad repeats. The effects of substituting leucine by phenylalanine and alanine by glycine on the self-assembly of coiled-coils were examined in minimalist antimicrobial peptides designed to form amphipathic alpha-helices....
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi961644f
更新日期:1997-08-05 00:00:00
abstract::The structure of an active mutant of (S)-mandelate dehydrogenase (MDH-GOX2) from Pseudomonas putida has been determined at 2.15 A resolution. The membrane-associated flavoenzyme (S)-mandelate dehydrogenase (MDH) catalyzes the oxidation of (S)-mandelate to give a flavin hydroquinone intermediate which is subsequently r...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi010938k
更新日期:2001-08-21 00:00:00
abstract::To identify the specific component(s) in the target membrane involved in fusion of vesicular stomatitis virus (VSV), we examined the interaction of the virus with human erythrocyte membranes with asymmetric and symmetric bilayer distributions of phospholipids. Fusion was monitored spectrofluorometrically by the octade...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00469a005
更新日期:1990-05-01 00:00:00
abstract::Neuroglobin (Ngb) is a recently discovered vertebrate heme protein that is expressed in the brain and can reversibly bind oxygen. Mammalian Ngb is involved in neuroprotection under oxidative stress conditions, such as ischemia and reperfusion. We previously demonstrated that human ferric Ngb binds to the alpha subunit...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi800286m
更新日期:2008-05-13 00:00:00
abstract::A series of antisense oligonucleotides (ASOs) were synthesized and tested to define the best target sites within an RNA transcript of collagen for effective inhibition of expression. The test system consisted of mouse NIH 3T3 fibroblasts that were stably transfected with a human minigene for procollagen I so that the ...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00202a024
更新日期:1994-09-13 00:00:00
abstract::The sensitivity of the fluorescence energy of 2-p-toluidinylnaphthalene-6-sulfonate (TNS) to apparent solvent polarity is considerably greater in methanol-water mixtures and lower primary alcohols than in higher primary alcohols or solvents containing no hydroxyl groups. It is suggested that the behavior of TNS in low...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00675a016
更新日期:1975-02-25 00:00:00
abstract::The mechanism of DNA polymerase beta-catalyzed nucleotidyl transfer consists of chemical steps involving primer 3' OH deprotonation, nucleophilic attack, and pyrophosphate leaving-group elimination, preceded by dNTP binding which induces a large-amplitude conformational change for Watson-Crick nascent base pairs. Ambi...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi7014162
更新日期:2008-01-22 00:00:00
abstract::Cytochrome c6 from Monoraphidium braunii, an 89-amino acid electron transfer protein, has been investigated by NMR in solution, in its oxidized form, at pH 7 and 300 K. By using a combination of COSY, TOCSY, and NOESY experiments, 84% of the proton resonances have been assigned. A total of 1668 experimental NOE constr...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi972765y
更新日期:1998-04-07 00:00:00
abstract::The actinomycin biosynthetic gene cluster of Streptomyces chrysomallus harbors two paralogous genes, acmI and acmL, encoding methyltransferases. To unveil their suspected role in the formation of 3-hydroxy-4-methyl-anthranilic acid (4-MHA), the building block of the actinomycin chromophore, each gene was expressed in ...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi101422r
更新日期:2010-11-16 00:00:00
abstract::The affinity of various carbohydrates for the galactose/N-acetylgalactosamine-specific lectin of the rabbit liver was assessed by determining the effect of these carbohydrates on the binding of [125I]asialoorosomucoid (125I-ASOR) by the lectin. To obtain the concentration of the inhibitor that causes 50% reduction in ...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00267a039
更新日期:1982-11-23 00:00:00
abstract::The kinetic mechanism of NADPH-cytochrome P450 oxidoreductase (P450R) has been determined for the model reaction with cytochrome c3+. Although initial velocity studies show parallel patterns, consistent with a classical (one-site) ping-pong mechanism that precludes the formation of a ternary NADPH-P450R-cytochrome c3+...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00206a002
更新日期:1994-10-11 00:00:00
abstract::The conformational states of N-acylalanine dithio esters, involving rotational isomers about the RC(=O)NH--CH(CH3) and NHCH(CH3)--C(=S) bonds, are defined and compared to those of N-acylglycine dithio esters. The structure of N-(p-nitrobenzoyl)-DL-alanine ethyl dithio ester has been determined by X-ray crystallographi...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00401a038
更新日期:1988-01-12 00:00:00
abstract::DNA-DNA interstrand cross-links are thought to be important for the cytotoxicity of many chemotherapeutic agents. To study this more definitively, adduct site-specific methods are used to construct a plasmid with a single nitrogen mustard interstrand cross-link (inter-HN2-pTZSV28). Replication efficiency (RE = [coloni...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi962778w
更新日期:1997-03-25 00:00:00
abstract::Glucose oxidation to CO2 was investigated in isolated perifused rat epididymal fat cells. Insulin stimulated rates of oxidation up to 30-fold. Multiple pulses of insulin or prolonged perifusion with the hormone led to a time-dependent desensitization of the cells. The action of insulin could be mimicked by H2O2. Rever...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00273a013
更新日期:1983-02-15 00:00:00
abstract::Transient kinetic data for D-xylose reduction with NADPH and NADPD and for xylitol oxidation with NADP+ catalyzed by recombinant C298A mutant human aldose reductase at pH 8 have been used to obtain estimates for each of the rate constants in the complete reaction mechanism as outlined for the wild-type enzyme in the p...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00044a013
更新日期:1995-11-07 00:00:00
abstract::The carbohydrate binding specificity of the seed lectin from Artocarpus integrifolia, artocarpin, has been elucidated by the enzyme-linked lectin absorbent assay [Misquith, S., et al (1994) J. Biol. Chem. 269, 30393-30401], wherein it was demonstrated to be a Man/Glc specific lectin with high affinity for the trisacch...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi000744p
更新日期:2000-09-05 00:00:00