Abstract:
:We have determined the nucleotide sequence and the derived amino acid sequence of the phosphoprotein-encoding region of the chicken vitellogenin III gene. The sequence of this minor vitellogenin could be aligned with exon 22 up to exon 27 of the previously sequenced major vitellogenin II gene (van het Schip et al., 1987). The exon 23 and 25 sequences are rich in serine codons (26% and 41%, respectively), and this region encodes at least one of the small egg yolk phosphoproteins. The major egg yolk phosphoprotein, phosvitin, is encoded by the analogous region in vitellogenin II. Comparison of the vitellogenin II and vitellogenin III sequences shows a great reduction in the size of the putative exon 23 of the latter (321 base pairs as opposed to 690). The number of serine codons is also drastically reduced from 124 in exon 23 of the vitellogenin II gene to 28 in vitellogenin III. The grouping of synonymous serine codons, as has hitherto been observed in sequenced vitellogenin phosphoproteins, has been maintained in vitellogenin III. A putative asparagine-linked N-glycosylation site which was conserved in the chicken vitellogenin II and the Xenopus laevis vitellogenin A2 gene, at the beginning of exon 23, is also present in vitellogenin III. The two chicken vitellogenins show a low conservation in the phosphoprotein-encoding region (average 33%, at the protein level) compared to that in the peripheral sequences (58% identity), which indicates that it is a rapidly evolving domain of the vertebrate vitellogenin gene.
journal_name
Biochemistryjournal_title
Biochemistryauthors
Byrne BM,de Jong H,Fouchier RA,Williams DL,Gruber M,Ab Gdoi
10.1021/bi00432a034subject
Has Abstractpub_date
1989-03-21 00:00:00pages
2572-7issue
6eissn
0006-2960issn
1520-4995journal_volume
28pub_type
杂志文章相关文献
BIOCHEMISTRY文献大全abstract::Enzymic catalysis proceeds via intermediates formed in the course of substrate conversion. Here, we directly detect key intermediates in thiamin diphosphate (ThDP)-dependent enzymes during catalysis using (1)H NMR spectroscopy. The quantitative analysis of the relative intermediate concentrations allows the determinat...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi034465o
更新日期:2003-07-08 00:00:00
abstract::The human transferrin receptor (TfR) has three N-linked oligosaccharides. A combination of site-directed mutagenesis and carbohydrate and protein chemistry was used to characterize the structures of the N-linked oligosaccharides and to map their locations. We find that the type of oligosaccharide at each position was ...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi962884y
更新日期:1997-04-29 00:00:00
abstract::Glycophorin A is a sialoglycoprotein isolated from human erythrocyte membranes which seems to exist as stable dimeric complexes in the presence of sodium dodecyl sulfate. When analyzed by dodecyl sulfate acrylamide electrophoresis this molecule forms two PAS-stainable bands (PAS-U and PAS-2) which are reversibly inter...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00650a028
更新日期:1976-03-09 00:00:00
abstract::The addition of 10(-9) M insulin to a suspension of rat liver plasma membranes increases the overall lipid microviscosity, eta, by about 10--20%. The effect is confined to physiological concentrations of the hormone and is highly specific. The specificity was demonstrated in experiments where insulin analogues were ad...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00570a009
更新日期:1979-02-06 00:00:00
abstract::Minor adducts, derived from the covalent binding of anti-benzo[a]pyrene-7,8-dihydroxy-9,10-epoxide to cellular DNA, may play an important role in generating mutations and initiating cancer. We have applied a combined NMR-computational approach including intensity based refinement to determine the solution structure of...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi991212f
更新日期:1999-08-17 00:00:00
abstract::Desosamine is a 3-(dimethylamino)-3,4,6-trideoxyhexose found, for example, in such macrolide antibiotics as erthyromycin, azithromycin, and clarithromycin. The efficacies of these macrolide antibiotics are markedly reduced in the absence of desosamine. In the bacterium Streptomyces venezuelae, six enzymes are required...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi700751d
更新日期:2007-08-07 00:00:00
abstract::Intramolecular excimerization of 1,3-di-1-pyrenylpropane [Py(3)Py] was used to assess the fluidity of sarcoplasmic reticulum membranes (SR); on the basis of the spectral data, the probe incorporates completely inside the membrane probably somewhere close to the polar head groups of phospholipid molecules, however not ...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00315a029
更新日期:1984-09-25 00:00:00
abstract::Thermodynamic equilibrium transition models in DSC are only applicable to reversible processes, but reversibility of the thermal transitions of proteins is comparatively rare because of intermolecular aggregation of denatured proteins and the degradation that occurs at high temperatures. The cupredoxin azurin from Pse...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi0157621
更新日期:2002-01-22 00:00:00
abstract::We have described earlier a chromatin-bound protease with unique specificity for histone H2A [Eickbush, T. H., Watson, D. K., & Moudrianakis, E. N. (1976) Cell (Cambridge, Mass.) 9, 785--792]. In the present study, we explore the nature of interactions that form and stabilize the enzyme-chromatin system by using the a...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00531a008
更新日期:1982-01-19 00:00:00
abstract::Illumination of envelope vesicles prepared from Halobacterium halobium cells causes translocation of protons from inside to outside, due to the light-induced cycling of bacteriorhodopsin. This process results in a pH gradient across the membranes, an electrical potential, and the movements of K+ and Na+. The electrica...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00655a017
更新日期:1976-05-18 00:00:00
abstract::Double homeodomain (DUX) proteins are encoded by a family of 3.3-kilobase repeated elements dispersed in the human genome. One of these elements named D4Z4 is found in a tandem repeat array on chromosome 4 that is partially deleted in facioscapulohumeral muscular dystrophy. We have evaluated the trafficking and mobili...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi047992w
更新日期:2005-02-22 00:00:00
abstract::A combination of mutagenesis, calorimetry, kinetics, and small-angle X-ray scattering (SAXS) has been used to study the mechanism of ligand binding energy propagation through human cytochrome P450 reductase (CPR). Remarkably, the energetics of 2',5'-ADP binding to R597 at the FAD-binding domain are affected by mutatio...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi700596s
更新日期:2007-07-17 00:00:00
abstract::The crystal structure of a cytochrome P450 from the thermoacidophile Picrophilus torridus, CYP231A2 (PTO1399), has been solved. This structure reveals a wide open substrate access channel. To better understand ligand-induced structural transitions in CYP231A2, protein-ligand interactions were investigated using 4-phen...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi702240k
更新日期:2008-02-19 00:00:00
abstract::Site-directed mutagenesis of the aspartate receptor of Salmonella typhimurium (Tars) at serine 68, a residue located within the aspartate binding pocket and at the subunit interface, identified this residue as an allosteric switch in this receptor. Substitutions at this position can affect both the type and degree of ...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi961481v
更新日期:1996-11-26 00:00:00
abstract::The PLL(PTE-like lactonase)-group of enzymes within the amidohydrolase superfamily hydrolyze N-acyl-homoserine lactones (AHLs) that are involved in bacterial quorum-sensing pathways. These enzymes possess the (beta/alpha)(8)-barrel fold and serve as attractive templates for in vitro evolution and engineering of quorum...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi9004045
更新日期:2009-05-26 00:00:00
abstract::The 70-kDa heat-shock protein (Hsp70) has been cloned and sequenced from bovine cardiac muscle. On the basis of sequence features, the gene corresponds to the cytoplasmic form of Hsp70. This cardiac Hsp70 cDNA clone has an open reading frame of 1926 bp coding for 641 amino acids and a predicted molecular mass of 70.25...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi049036k
更新日期:2004-10-26 00:00:00
abstract::We have purified and characterized a novel 30-kDa glycoprotein (gp30) with TGF alpha-like properties secreted from the estrogen receptor negative breast cancer cell line MDA-MB-231. This factor was immunoprecipitated by an anti-TGF alpha polyclonal antibody and also had TGF alpha-like biological activity, as assayed b...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00147a018
更新日期:1992-08-18 00:00:00
abstract::Activated receptors with a tyrosine kinase activity induce a variety of responses like changes in the differentiation and mitogenic status of cells. These responses are mediated in part by p21ras. Some of these activated receptors induce in certain cell types a pronounced, but transient, increase in Ras-GTP. We have s...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00189a053
更新日期:1994-06-14 00:00:00
abstract::Anatoxin-a and homoanatoxin-a are two potent cyanobacterial neurotoxins. We recently reported the identification of the gene cluster responsible for the biosynthesis of these toxins as well as the in-vitro reconstitution of the first steps of this biosynthesis. We now report experimental evidence supporting the propos...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi200892a
更新日期:2011-08-23 00:00:00
abstract::5-HT(2A) serotonin receptors represent the principal molecular targets for LSD-like hallucinogens and atypical antipsychotic drugs. It has been proposed that a dysregulation of 5-HT(2A) receptor-mediated signaling may contribute to the pathogenesis of schizophrenia and related diseases. A major mechanism for the atten...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi035061z
更新日期:2003-09-16 00:00:00
abstract::The mitogen-activated protein kinases (MAPKs) are key components of cellular signal transduction pathways, which are down-regulated by the MAPK phosphatases (MKPs). Catalytic activity of the MKPs is controlled both by their ability to recognize selective MAPKs and by allosteric activation upon binding to MAPK substrat...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/acs.biochem.7b00827
更新日期:2017-11-21 00:00:00
abstract::The chemical mechanism of phosphodiester bond hydrolysis catalyzed by a junction-resolving enzyme has been investigated. Endonuclease I of phage T7 is a member of the nuclease superfamily of proteins that include many restriction enzymes, and the structure of the active site is very similar to that of BglI in particul...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi0523254
更新日期:2006-03-28 00:00:00
abstract::Retinal rod and cone cGMP phosphodiesterases (PDE6 family) function as the effector enzyme in the vertebrate visual transduction cascade. The activity of PDE6 catalytic subunits is controlled by the Pgamma-subunits. In addition to the inhibition of cGMP hydrolysis at the catalytic sites, Pgamma is known to stimulate a...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi015935m
更新日期:2002-03-26 00:00:00
abstract::Human serum albumin (HSA) is the most abundant protein in the blood plasma and is involved in the transport of metal ions. Four metal-binding sites with different specificities have been described in HSA: (i) the N-terminal site provided by Asp1, Ala2, and His3, (ii) the site at the reduced Cys34, (iii) site A, includ...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi061783p
更新日期:2007-02-27 00:00:00
abstract::The interaction of bee venom melittin with dimyristolphosphatidylcholine (DMPC) selectively deuteriated in the choline head group has been studied by deuterium and phosphorus-31 nuclear magnetic resonance (NMR) spectroscopy. The action of residual phospholipase A2 in melittin samples resulted in mixtures of DMPC and i...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00392a033
更新日期:1987-09-08 00:00:00
abstract::In order to clarify the functional and structural role of intra- and intersubunit hydrogen bonds in human hemoglobin (Hb A), we prepared two artificial beta chain mutant hemoglobins by site-directed mutagenesis. The mutant Hb Phe-37 beta, in which Trp-37 beta is replaced by Phe to remove the intersubunit hydrogen bond...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00127a030
更新日期:1992-03-31 00:00:00
abstract::Nitric oxide synthases (NOSs) are proposed to generate NO and citrulline from L-arginine in two steps: initial N-hydroxylation to generate Nomega-hydroxyarginine (NOHA) followed by a three-electron oxidation of the hydroxylated nitrogen to form products. Both steps consume NADPH and may involve heme iron-based activat...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi971414g
更新日期:1997-09-09 00:00:00
abstract::Cholesterol (Chol) in phosphatidylcholine large unilamellar vesicles (PC LUV) modulated interaction of the bilayers with a class A amphipathic peptide, Ac-18A-NH2: Chol increased the peptide binding capacity and reduced the affinity together with the peptide-induced leakage of calcein from LUV. Similar effects of Chol...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi011885+
更新日期:2002-03-26 00:00:00
abstract::X-ray diffraction measurements show that in contrast to the purple membrane, the bacteriorhodopsin molecules are not organized in a hexagonal lattice in the deionized blue membrane. Addition of Ca2+ restores both the purple color and the normal (63 A) hexagonal protein lattice. In the blue state, the circular dichrois...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00449a031
更新日期:1989-11-14 00:00:00
abstract::The periplasmic nitrate reductase (NapAB), a member of the DMSO reductase superfamily, catalyzes the first step of the denitrification process in bacteria. In this heterodimer, a di-heme NapB subunit is associated to the catalytic NapA subunit that binds a [4Fe-4S] cluster and a bis(molybdopterin guanine dinucleotide)...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi700928m
更新日期:2007-08-28 00:00:00