Spectral properties of environmentally sensitive probes associated with horseradish peroxidase.

abstract：:Factor XIIIa (a2') is a homodimeric transglutaminase that is formed via limited alpha-thrombin-catalyzed proteolysis of the platelet (a2) or plasma (a2b2) factor XIII zymogen in a reaction that results in proteolytic removal of a 37-aminoacyl residue peptide from the N-terminus of the a chains and exposure of the acti...

journal_title：Biochemistry

authors： Hornyak TJ,Shafer JA

更新日期：1992-01-21 00:00:00

abstract：:Hydroxyl radical induced formation of a DNA-protein cross-link involving thymine and tyrosine in nucleohistone is described. Hydroxyl radicals were generated in N2O-saturated aqueous solution by ionizing radiation. Samples of nucleohistone were hydrolyzed with HCl and trimethylsilylated. Analysis of irradiated samples...

journal_title：Biochemistry

authors： Dizdaroglu M,Gajewski E,Reddy P,Margolis SA

更新日期：1989-04-18 00:00:00

abstract：:Monoclonal antibodies directed against epitopes on each of the five subunits (alpha, beta, gamma, delta, and epsilon) of the Escherichia coli F1 ATPase (ECF1) have been prepared and used to localize the subunits in the enzyme complex. Fab' fragments, prepared by pepsin digestion of the antibodies, were bound to ECF1 a...

journal_title：Biochemistry

authors： Gogol EP,Aggeler R,Sagermann M,Capaldi RA

更新日期：1989-05-30 00:00:00

abstract：:Treatment with cholesterol oxidases has shown that cholesterol is heterogeneously distributed in brush border membranes isolated from the apical domain of the renal and intestinal epithelial cells [Bloj, B., & Zilversmit, D. B. (1982) J. Biol. Chem. 257, 7608-7614; El Yandouzi, E. H., & Le Grimellec, C. (1992) Biochem...

journal_title：Biochemistry

authors： el Yandouzi EH,Zlatkine P,Moll G,Le Grimellec C

更新日期：1994-03-01 00:00:00

abstract：:A series of truncated forms of His(6)-tagged gp91phox were expressed, solubilized, and purified in the presence of 30 microM FAD. The truncated gp91phox with the longest sequence in the C-terminal region (221-570) (gp91C) showed the highest activity (turnover rate, 0.92) for NADPH diaphorase in the presence of either ...

journal_title：Biochemistry

authors： Nisimoto Y,Ogawa H,Miyano K,Tamura M

更新日期：2004-07-27 00:00:00

abstract：:Fibronectin (FN) is an extracellular matrix (ECM) protein found soluble in corporal fluids or as an insoluble fibrillar component incorporated in the ECM. This phenomenon implicates structural changes that expose FN binding sites and activate the protein to promote intermolecular interactions with other FN. We have in...

journal_title：Biochemistry

authors： Patel S,Chaffotte AF,Goubard F,Pauthe E

更新日期：2004-02-17 00:00:00

abstract：:Pseudomonas aeruginosa PA4872 was identified by sequence analysis as a structurally and functionally novel member of the PEP mutase/isocitrate lyase superfamily and therefore targeted for investigation. Substrate screens ruled out overlap with known catalytic functions of superfamily members. The crystal structure of ...

journal_title：Biochemistry

authors： Narayanan BC,Niu W,Han Y,Zou J,Mariano PS,Dunaway-Mariano D,Herzberg O

更新日期：2008-01-08 00:00:00

abstract：:Lysophosphatidic acid (1-acyl-sn-glycero-3-phosphate or LPA) is a phospholipid mediator displaying numerous and widespread biological activities and thought to act via G-protein-coupled receptors. Here we have studied the effects on human platelets of a number of LPA analogues, including two enantiomers of both N-palm...

journal_title：Biochemistry

authors： Gueguen G,Gaigé B,Grévy JM,Rogalle P,Bellan J,Wilson M,Klaébé A,Pont F,Simon MF,Chap H

更新日期：1999-06-29 00:00:00

abstract：:A strategy to detect P-glycoprotein (P-gp) on cell membrane and quantify the cell number using electrochemical immunoassay was developed by effective surface immunoreactions and immobilization of cells on a highly hydrophilic interface, which was constructed by adsorption of colloidal gold nanoparticles on a methoxysi...

journal_title：Biochemistry

authors： Du D,Ju H,Zhang X,Chen J,Cai J,Chen H

更新日期：2005-08-30 00:00:00

abstract：:Pantothenate synthetase (PS, EC 6.3.2.1) is the last enzyme in the pantothenate biosynthesis pathway, a metabolic pathway identified as a potential target for new antimicrobials. PS catalyzes the ATP-dependent condensation of pantoate and beta-alanine to form pantothenate. Here we report the overexpression, purificati...

journal_title：Biochemistry

authors： Satoh A,Konishi S,Tamura H,Stickland HG,Whitney HM,Smith AG,Matsumura H,Inoue T

更新日期：2010-08-03 00:00:00

abstract：:15N NMR spectroscopy was used to examine the active-site histidyl residue of alpha-lytic protease in peptide boronic acid inhibitor complexes. Two distinct types of complexes were observed: (1) Boronic acids that are analogues of substrates form complexes in which the active-site imidazole ring is protonated and both ...

journal_title：Biochemistry

authors： Bachovchin WW,Wong WY,Farr-Jones S,Shenvi AB,Kettner CA

更新日期：1988-10-04 00:00:00

abstract：:HcbA1 is a unique flavoenzyme that catalyzes the first step in the bacterial hexachlorobenzene catabolic pathway. Here we report in vitro reconstitution of the HcbA1-catalyzed reaction. Detailed mechanistic studies provide evidence for nucleophilic aromatic substitution and flavin-N5-oxide formation. ...

journal_title：Biochemistry

authors： Adak S,Begley TP

更新日期：2019-03-05 00:00:00

abstract：:Both the male and the female of Mastomys natalensis, an African rat, have high levels of nerve growth factor (NGF) in their submaxillary glands. Mastomys high molecular weight (HMW) NGF was purified by gel filtration and ion-exchange chromatography and was compared with HMW NGF from the male mouse submaxillary gland. ...

journal_title：Biochemistry

authors： Darling TL,Fahnestock M

更新日期：1988-09-06 00:00:00

abstract：:The chemical mechanism of the phosphoribosyltransferases (PRTases), although largely unknown, may proceed either via a concerted direct-transfer mechanism or with a two-step mechanism involving a carboxonium-like intermediate. To study this question, we have cloned the Salmonella typhimurium pyrE gene, coding for the ...

journal_title：Biochemistry

authors： Bhatia MB,Vinitsky A,Grubmeyer C

更新日期：1990-11-20 00:00:00

abstract：:The behavior of photosystem II (PSII) reactions was investigated under conditions of decreasing water content by the addition of increasing concentrations of ethylene glycol (EG). The photosynthetic activities were measured for PSII samples either directly in aqueous solutions of EG or in the standard buffer medium fo...

journal_title：Biochemistry

authors： Hillier W,Lukins P,Seibert M,Wydrzynski T

更新日期：1997-01-07 00:00:00

abstract：:The 35000-Mr beta-adrenergic receptor mRNA binding protein (beta ARB) is induced by beta-adrenergic agonists and binds to G-protein-linked receptor mRNAs that exhibit agonist-induced destabilization. Recently, we identified a 20-nucleotide, AU-rich region in the 3'-untranslated region of the hamster beta 2-adrenergic ...

journal_title：Biochemistry

authors： Tholanikunnel BG,Raymond JR,Malbon CC

更新日期：1999-11-23 00:00:00

abstract：:The organometallic anticancer agent titanocene dichloride, Cp(2)TiCl(2), is now in phase II clinical trials as an anticancer drug, but its mechanism of action is poorly understood. We show here that the interactions of Cp(2)TiCl(2) with human serum transferrin (hTF) and that of Ti(2)-hTF with adenosine triphosphate (A...

journal_title：Biochemistry

authors： Guo M,Sun H,McArdle HJ,Gambling L,Sadler PJ

更新日期：2000-08-22 00:00:00

abstract：:If cytochrome c oxidase is subjected to pressure during the aerobic steady state, large spectral changes are apparent. These seem to be associated with the inhibition of electron transport within the oxidase. The volume change for the transition is about 80 mL/mol. When the oxidase in the aerobic steady state, with po...

journal_title：Biochemistry

authors： Kornblatt JA,Hui Bon Hoa G,Heremans K

更新日期：1988-07-12 00:00:00

abstract：:Understanding the mechanisms of free energy transfer in metabolism is fundamental to understanding how the chemical forces that sustain the molecular organization of the cell are distributed. Recent studies of molecular motors (1-3) and ATP-driven proton transport (4-6) describe how chemical potential is transferred a...

journal_title：Biochemistry

authors： Wei J,Tang QX,Varlamova O,Roche C,Lee R,Leyh TS

更新日期：2002-07-02 00:00:00

abstract：:Membrane-bound phosphodiesterase 6 (PDE6) plays an important role in visual signal transduction by regulating cGMP levels in rod photoreceptor cells. Our understanding of PDE6 catalysis and structure suffers from inadequate characterization of the α and β subunit catalytic core, interactions of the core with two intri...

journal_title：Biochemistry

authors： Baker BY,Palczewski K

更新日期：2011-11-08 00:00:00

abstract：:Taurine/alpha-ketoglutarate (alphaKG) dioxygenase (TauD), an archetype alphaKG-dependent hydroxylase, is a non-heme mononuclear Fe(II) enzyme that couples the oxidative decarboxylation of alphaKG with the conversion of taurine to aminoacetaldehyde and sulfite. The crystal structure of taurine-alphaKG-Fe(II)TauD is kno...

journal_title：Biochemistry

authors： Grzyska PK,Ryle MJ,Monterosso GR,Liu J,Ballou DP,Hausinger RP

更新日期：2005-03-15 00:00:00

abstract：:S100A1, a member of the S100 protein family, is an EF-hand containing Ca(2+)-binding protein (93 residues per subunit) with noncovalent interactions at its dimer interface. Each subunit of S100A1 has four alpha-helices and a small antiparallel beta-sheet consistent with two helix-loop-helix calcium-binding domains [Ba...

journal_title：Biochemistry

authors： Rustandi RR,Baldisseri DM,Inman KG,Nizner P,Hamilton SM,Landar A,Landar A,Zimmer DB,Weber DJ

更新日期：2002-01-22 00:00:00

abstract：:Site-directed mutants of human carbonic anhydrase III were used to examine the role of Thr-199 and its interaction with Phe-198 in the catalyzed hydration of CO2. Threonine-199 is a hydrogen bond acceptor for the zinc-bound water, and Phe-198 forms part of the hydrophobic side of the active-site cavity of carbonic anh...

journal_title：Biochemistry

authors： Chen X,Tu C,LoGrasso PV,Laipis PJ,Silverman DN

更新日期：1993-08-10 00:00:00

abstract：:Chloramphenicol acetyltransferase (CAT) catalyzes the acetyl-CoA-dependent acetylation of chloramphenicol by a ternary complex mechanism with a rapid equilibrium and essentially random order of addition of substrates. Such a kinetic mechanism for a two-substrate reaction provides an opportunity to compare the affinity...

journal_title：Biochemistry

authors： Ellis J,Bagshaw CR,Shaw WV

更新日期：1991-11-05 00:00:00

abstract：:Flavin-dependent histone demethylases catalyze the posttranslational oxidative demethylation of mono- and dimethylated lysine residues, producing formaldehyde and hydrogen peroxide in addition to the corresponding demethylated protein. In vivo, histone demethylase LSD1 (KDM1; BCH110) is a component of the multiprotein...

journal_title：Biochemistry

authors： Hwang S,Schmitt AA,Luteran AE,Toone EJ,McCafferty DG

更新日期：2011-02-01 00:00:00

abstract：:Deuterium magnetic resonance spectra (55.26 MHz) of cholesterol-3 alpha-d1 and epicholesterol-3 beta-d1 in dipalmitoylglycerophosphocholine (DPPC) liposomes were measured as a function of sterol-to-phospholipid ratio below (24 degrees C) and above (60 degrees C) the phase transition temperature of DPPC. From the quadr...

journal_title：Biochemistry

authors： Murari R,Murari MP,Baumann WJ

更新日期：1986-03-11 00:00:00

abstract：:The understudied nonribosomal-peptide-synthetase-independent siderophore (NIS) synthetase family has been increasingly associated with virulence in bacterial species due to its key role in the synthesis of hydroxamate and carboxylate "stealth" siderophores. We have identified a model family member, DesD, from Streptom...

journal_title：Biochemistry

authors： Hoffmann KM,Goncuian ES,Karimi KL,Amendola CR,Mojab Y,Wood KM,Prussia GA,Nix J,Yamamoto M,Lathan K,Orion IW

更新日期：2020-09-22 00:00:00

abstract：:Monomeric sarcosine oxidase (MSOX) is a prototypical member of a recently recognized family of amine-oxidizing enzymes that all contain covalently bound flavin. Mutation of the covalent flavin attachment site in MSOX produces a catalytically inactive apoprotein (apoCys315Ala) that forms an unstable complex with FAD (K...

journal_title：Biochemistry

authors： Hassan-Abdallah A,Zhao G,Jorns MS

更新日期：2006-08-08 00:00:00

abstract：:Previous studies have demonstrated that lung-specific proteins are associated with surfactant lipids, particularly the highly surface active subfraction known as tubular myelin. We have isolated a surfactant-associated protein complex with molecular weight components of 36 000, 32 000, and 28 000 and reassembled it wi...

journal_title：Biochemistry

authors： Hawgood S,Benson BJ,Hamilton RL Jr

更新日期：1985-01-01 00:00:00

abstract：:While the insertion of a hydrophobic peptide or membrane protein segment into the bilayer can be spontaneous and driven mainly by the hydrophobic effect, anionic lipids, which comprise ca. 20% of biological membranes, provide a source of electrostatic attractions for binding of proteins/peptides into membranes. To unr...

journal_title：Biochemistry

authors： Liu LP,Deber CM

更新日期：1997-05-06 00:00:00