Effects of a surfactant-associated protein and calcium ions on the structure and surface activity of lung surfactant lipids.

Abstract:

:Previous studies have demonstrated that lung-specific proteins are associated with surfactant lipids, particularly the highly surface active subfraction known as tubular myelin. We have isolated a surfactant-associated protein complex with molecular weight components of 36 000, 32 000, and 28 000 and reassembled it with protein-free lung surfactant lipids prepared as small unilamellar liposomes. The effects of divalent cations on the structure and surface activity of this protein-lipid mixture were investigated by following (1) the state of lipid dispersion by changes in turbidity and by electron microscopy and (2) the ability of the surfactant lipids to form a surface film from an aqueous subphase at 37 degrees C. The protein complex markedly increased the rate of Ca2+-induced surfactant-lipid aggregation. Electron microscopy demonstrated transformation of the small unilamellar liposomes (median diameter 440 A) into large aggregates. The threshold Ca2+ concentration required for rapid lipid aggregation was reduced from 13 to 0.5 mM by the protein complex. This protein-facilitated lipid aggregation did not occur if Mg2+ was the only divalent cation present. Similarly, 5 mM Ca2+ but not 5 mM Mg2+ improved the ability of the protein-lipid mixture to form a surface film at 37 degrees C. Extensive aggregation of the surfactant lipids without protein by 20 mM Ca2+ or 20 mM Mg2+ did not promote rapid surface film formation. These results add to the growing evidence that specific Ca2+-protein-lipid interactions are important in determining both the structure and function of extracellular lung surfactant fractions.

journal_name

Biochemistry

journal_title

Biochemistry

authors

Hawgood S,Benson BJ,Hamilton RL Jr

doi

10.1021/bi00322a026

subject

Has Abstract

pub_date

1985-01-01 00:00:00

pages

184-90

issue

1

eissn

0006-2960

issn

1520-4995

journal_volume

24

pub_type

杂志文章
  • Structural and spectroscopic studies of the copper site of stellacyanin.

    abstract::The structure of the copper site in oxidized and reduced Rhus vernicifera stellacyanin has been studied by X-ray absorption (XAFS) spectroscopy at different pH values. Data for the oxidized protein are consistent with the fourth ligand being an O- or N-donating ligand rather than a cysteine from the disulfide bridge. ...

    journal_title:Biochemistry

    pub_type: 杂志文章

    doi:10.1021/bi00001a026

    authors: Strange RW,Reinhammar B,Murphy LM,Hasnain SS

    更新日期:1995-01-10 00:00:00

  • High-affinity insulin binding: insulin interacts with two receptor ligand binding sites.

    abstract::The interaction of insulin with its receptor is complex. Kinetic and equilibrium binding studies suggest coexistence of high- and low-affinity binding sites or negative cooperativity. These phenomena and high-affinity interactions are dependent on the dimeric structure of the receptor. Structure-function studies of in...

    journal_title:Biochemistry

    pub_type: 杂志文章

    doi:10.1021/bi801693h

    authors: Whittaker L,Hao C,Fu W,Whittaker J

    更新日期:2008-12-02 00:00:00

  • Hemoglobin of the Antarctic fishes Trematomus bernacchii and Trematomus newnesi: structural basis for the increased stability of the liganded tetramer relative to human hemoglobin.

    abstract::Hemoglobins extracted from fishes that live in temperate waters show little or no dissociation even in the liganded form, unlike human hemoglobin (HbA). To establish whether cold adaptation influences the tendency to dissociate, the dimer-tetramer association constants (L(2,4)) of the carbonmonoxy derivatives of repre...

    journal_title:Biochemistry

    pub_type: 杂志文章

    doi:10.1021/bi002297j

    authors: Giangiacomo L,D'Avino R,di Prisco G,Chiancone E

    更新日期:2001-03-13 00:00:00

  • Primary photoreaction of photoactive yellow protein studied by subpicosecond-nanosecond spectroscopy.

    abstract::The primary photochemical event of photoactive yellow protein (PYP) was studied by laser flash photolysis experiments on a subpicosecond-nanosecond time scale. PYP was excited by a 390-nm pulse, and the transient difference absorption spectra were recorded by a multichannel spectrometer for a more reliable spectral an...

    journal_title:Biochemistry

    pub_type: 杂志文章

    doi:10.1021/bi002437p

    authors: Imamoto Y,Kataoka M,Tokunaga F,Asahi T,Masuhara H

    更新日期:2001-05-22 00:00:00

  • Gal repressor-operator-HU ternary complex: pathway of repressosome formation.

    abstract::DNA transaction reactions require formation of nucleoprotein complexes that involve multifaceted DNA-protein and protein-protein interactions. Genetic and biochemical studies suggested that the higher order Gal repressosome structure, which governs the transcription of two tandem galpromoters in Escherichia coli, invo...

    journal_title:Biochemistry

    pub_type: 杂志文章

    doi:10.1021/bi047720t

    authors: Roy S,Dimitriadis EK,Kar S,Geanacopoulos M,Lewis MS,Adhya S

    更新日期:2005-04-12 00:00:00

  • Selective inhibition by methoxyamine of the apurinic/apyrimidinic endonuclease activity associated with pyrimidine dimer-DNA glycosylases from Micrococcus luteus and bacteriophage T4.

    abstract::The UV endonucleases [endodeoxyribonuclease (pyrimidine dimer), EC 3.1.25.1] from Micrococcus luteus and bacteriophage T4 possess two catalytic activities specific for the site of cyclobutane pyrimidine dimers in UV-irradiated DNA: a DNA glycosylase that cleaves the 5'-glycosyl bond of the dimerized pyrimidines and an...

    journal_title:Biochemistry

    pub_type: 杂志文章

    doi:10.1021/bi00386a011

    authors: Liuzzi M,Weinfeld M,Paterson MC

    更新日期:1987-06-16 00:00:00

  • Cytochrome P450 from Photobacterium profundum SS9, a piezophilic bacterium, exhibits a tightened control of water access to the active site.

    abstract::We report cloning, expression in Escherichia coli, and purification of cytochrome P450 from a deep-sea bacterium Photobacterium profundum strain SS9 (P450-SS9). The enzyme, which is predominately high spin (86%) in the absence of any added ligand, binds fatty acids and their derivatives and exhibits the highest affini...

    journal_title:Biochemistry

    pub_type: 杂志文章

    doi:10.1021/bi101466y

    authors: Sineva EV,Davydov DR

    更新日期:2010-12-21 00:00:00

  • Ethanol-perturbed amyloidogenic self-assembly of insulin: looking for origins of amyloid strains.

    abstract::A model cosolvent, ethanol, has profound and diversified effects on the amyloidogenic self-assembly of insulin, yielding spectroscopically and morphologically distinguishable forms of beta-aggregates. The alcohol reduces hydrodynamic radii of insulin molecules, decreases enthalpic costs associated with aggregation-pro...

    journal_title:Biochemistry

    pub_type: 杂志文章

    doi:10.1021/bi050281t

    authors: Dzwolak W,Grudzielanek S,Smirnovas V,Ravindra R,Nicolini C,Jansen R,Loksztejn A,Porowski S,Winter R

    更新日期:2005-06-28 00:00:00

  • Membrane interaction of disease-related dynorphin A variants.

    abstract::The membrane interaction properties of two single-residue variants, R6W and L5S, of the 17-amino acid neuropeptide dynorphin A (DynA) were studied by circular dichroism (CD) and nuclear magnetic resonance (NMR) spectroscopy. Corresponding gene mutations have recently been discovered in humans and causatively linked to...

    journal_title:Biochemistry

    pub_type: 杂志文章

    doi:10.1021/bi4004205

    authors: Björnerås J,Gräslund A,Mäler L

    更新日期:2013-06-18 00:00:00

  • Factors participating in the liver-specific expression of the human apolipoprotein A-II gene and their significance for transcription.

    abstract::We have shown previously that the hepatic and intestinal transcription of the human apolipoprotein A-II (apoA-II) gene in cell cultures is controlled by a complex set of regulatory elements A-N [Chambaz, et al. (1991) J. Biol. Chem. 266, 11676-11685; Cardot, et al. (1991) J. Biol. Chem. 266, 24460-24470]. In the prese...

    journal_title:Biochemistry

    pub_type: 杂志文章

    doi:10.1021/bi00086a013

    authors: Cardot P,Chambaz J,Kardassis D,Cladaras C,Zannis VI

    更新日期:1993-09-07 00:00:00

  • The N-terminal 1000 residues of apolipoprotein B associate with microsomal triglyceride transfer protein to create a lipid transfer pocket required for lipoprotein assembly.

    abstract::Apolipoprotein (apo) B, the major protein component of the atherogenic low-density lipoprotein (LDL), has a pentapartite structure, NH2-betaalpha1-beta1-alpha2-beta2-alpha3-COOH, the beta domains containing multiple amphipathic beta strands and the alpha domains containing multiple amphipathic alpha helixes. We recent...

    journal_title:Biochemistry

    pub_type: 杂志文章

    doi:10.1021/bi011757l

    authors: Dashti N,Gandhi M,Liu X,Lin X,Segrest JP

    更新日期:2002-06-04 00:00:00

  • Modulating Integrin αIIbβ3 Activity through Mutagenesis of Allosterically Regulated Intersubunit Contacts.

    abstract::Integrin αIIbβ3, a transmembrane heterodimer, mediates platelet aggregation when it switches from an inactive to an active ligand-binding conformation following platelet stimulation. Central to regulating αIIbβ3 activity is the interaction between the αIIb and β3 extracellular stalks, which form a tight heterodimer in...

    journal_title:Biochemistry

    pub_type: 杂志文章

    doi:10.1021/acs.biochem.9b00430

    authors: Tan SK,Fong KP,Polizzi NF,Sternisha A,Slusky JSG,Yoon K,DeGrado WF,Bennett JS

    更新日期:2019-07-30 00:00:00

  • Understanding functional divergence in proteins by studying intragenomic homologues.

    abstract::Studies of intragenomic homologues in bacterial genomes can provide valuable insights into functional divergence. Three GTP cyclohydrolase II homologues in the Streptomyces coelicolor genome have been shown to catalyze two related but distinct reactions [Spoonamore, J. E., Dahlgran, A. L., Jacobsen, N. E., and Bandari...

    journal_title:Biochemistry

    pub_type: 杂志文章

    doi:10.1021/bi702263z

    authors: Spoonamore JE,Bandarian V

    更新日期:2008-02-26 00:00:00

  • Conformation-sensitive steroid and fatty acid sites in the transmembrane domain of the nicotinic acetylcholine receptor.

    abstract::The mechanism by which some hydrophobic molecules such as steroids and free fatty acids (FFA) act as noncompetitive inhibitors of the nicotinic acetylcholine receptor (AChR) is still not known. In the present work, we employ Förster resonance energy transfer (FRET) between the intrinsic fluorescence of membrane-bound ...

    journal_title:Biochemistry

    pub_type: 杂志文章

    doi:10.1021/bi061388z

    authors: Nievas GA,Barrantes FJ,Antollini SS

    更新日期:2007-03-20 00:00:00

  • A novel pentasaccharide sequence GlcA(3-sulfate)(beta1-3)GalNAc(4-sulfate)(beta1-4)(Fuc alpha1-3)GlcA(beta1-3)GalNAc(4-sulfate) in the oligosaccharides isolated from king crab cartilage chondroitin sulfate K and its differential susceptibility to chondroi

    abstract::Novel sulfated tetrasaccharide structures containing 3-O-sulfated GlcA were isolated recently from king crab cartilage chondroitin sulfate K [Sugahara, K., Tanaka, Y., Yamada, S., Seno, N., Kitagawa, H., Haslam, S. M., Morris, H. R., & Dell, A. (1996) J. Biol. Chem. 271, 26745-26754]. In this study, we prepared a seri...

    journal_title:Biochemistry

    pub_type: 杂志文章

    doi:10.1021/bi962740j

    authors: Kitagawa H,Tanaka Y,Yamada S,Seno N,Haslam SM,Morris HR,Dell A,Sugahara K

    更新日期:1997-04-01 00:00:00

  • Identification of the C=O stretching vibrations of FMN and peptide backbone by 13C-labeling of the LOV2 domain of Adiantum phytochrome3.

    abstract::Phototropin, a blue-light photoreceptor in plants, has two FMN-binding domains named LOV1 and LOV2. We previously observed temperature-dependent FTIR spectral changes in the C=O stretching region (amide-I vibrational region of the peptide backbone) for the LOV2 domain of Adiantum phytochrome3 (phy3-LOV2), suggesting p...

    journal_title:Biochemistry

    pub_type: 杂志文章

    doi:10.1021/bi061837v

    authors: Iwata T,Nozaki D,Sato Y,Sato K,Nishina Y,Shiga K,Tokutomi S,Kandori H

    更新日期:2006-12-26 00:00:00

  • Conformational states of N-acylalanine dithio esters: correlation of resonance Raman spectra with structures.

    abstract::The conformational states of N-acylalanine dithio esters, involving rotational isomers about the RC(=O)NH--CH(CH3) and NHCH(CH3)--C(=S) bonds, are defined and compared to those of N-acylglycine dithio esters. The structure of N-(p-nitrobenzoyl)-DL-alanine ethyl dithio ester has been determined by X-ray crystallographi...

    journal_title:Biochemistry

    pub_type: 杂志文章

    doi:10.1021/bi00401a038

    authors: Lee H,Angus RH,Storer AC,Varughese KI,Carey PR

    更新日期:1988-01-12 00:00:00

  • Conformational heterogeneity at position U37 of an all-RNA hairpin ribozyme with implications for metal binding and the catalytic structure of the S-turn.

    abstract::The hairpin ribozyme is an RNA enzyme that performs site-specific phosphodiester bond cleavage between nucleotides A-1 and G+1 within its cognate substrate. Previous functional studies revealed that the minimal hairpin ribozyme exhibited "gain-of-function" cleavage properties resulting from U39C or U39 to propyl linke...

    journal_title:Biochemistry

    pub_type: 杂志文章

    doi:10.1021/bi051550i

    authors: Alam S,Grum-Tokars V,Krucinska J,Kundracik ML,Wedekind JE

    更新日期:2005-11-08 00:00:00

  • Phosphorylation of Ser111 in Rab8a Modulates Rabin8-Dependent Activation by Perturbation of Side Chain Interaction Networks.

    abstract::GTPases are key players during cellular signaling. Phosphorylation of Rab proteins, which belong to the Ras superfamily of small GTPases regulating intracellular transport, has been implicated in the pathogenesis of Parkinson's disease. For Rab8a, it was shown that serine 111 phosphorylation (pS111) is dependent on th...

    journal_title:Biochemistry

    pub_type: 杂志文章

    doi:10.1021/acs.biochem.9b00516

    authors: Pourjafar-Dehkordi D,Vieweg S,Itzen A,Zacharias M

    更新日期:2019-08-20 00:00:00

  • Molecular structure of WlbB, a bacterial N-acetyltransferase involved in the biosynthesis of 2,3-diacetamido-2,3-dideoxy-D-mannuronic acid .

    abstract::The pathogenic bacteria Pseudomonas aeruginosa and Bordetella pertussis contain in their outer membranes the rare sugar 2,3-diacetamido-2,3-dideoxy-d-mannuronic acid. Five enzymes are required for the biosynthesis of this sugar starting from UDP-N-acetylglucosamine. One of these, referred to as WlbB, is an N-acetyltra...

    journal_title:Biochemistry

    pub_type: 杂志文章

    doi:10.1021/bi1005738

    authors: Thoden JB,Holden HM

    更新日期:2010-06-08 00:00:00

  • Site-directed mutagenesis of kappa-bungarotoxin: implications for neuronal receptor specificity.

    abstract::Postsynaptic polypeptide neurotoxins isolated from the venoms of elapid and hydrophid snakes exhibit the ability to bind selectively to and inhibit different types of receptors that function in nerve signal transmission. On the basis of their amino acid sequences and three-dimensional structures, these neurotoxins are...

    journal_title:Biochemistry

    pub_type: 杂志文章

    doi:10.1021/bi00179a011

    authors: Fiordalisi JJ,al-Rabiee R,Chiappinelli VA,Grant GA

    更新日期:1994-04-05 00:00:00

  • Peroxynitrite induces long-lived tyrosyl radical(s) in oxyhemoglobin of red blood cells through a reaction involving CO2 and a ferryl species.

    abstract::Peroxynitrite-mediated oxidative chemistry is currently the subject of intense investigation owing to the toxic side effects associated with nitric oxide overproduction. Using direct electron spin resonance spectroscopy (ESR) at 37 degrees C, we observed that in human erythrocytes peroxynitrite induced a long-lived si...

    journal_title:Biochemistry

    pub_type: 杂志文章

    doi:10.1021/bi982311g

    authors: Minetti M,Scorza G,Pietraforte D

    更新日期:1999-02-16 00:00:00

  • L30A Mutation of Phospholemman Mimics Effects of Cardiac Glycosides in Isolated Cardiomyocytes.

    abstract::To determine if mutations introduced into phospholemman (PLM) could increase the level of PLM-Na,K-ATPase (NKA) binding, we performed scanning mutagenesis of the transmembrane domain of PLM and measured Förster resonance energy transfer (FRET) between each mutant and NKA. We observed an increased level of binding to N...

    journal_title:Biochemistry

    pub_type: 杂志文章

    doi:10.1021/acs.biochem.6b00633

    authors: Himes RD,Smolin N,Kukol A,Bossuyt J,Bers DM,Robia SL

    更新日期:2016-11-08 00:00:00

  • Cavitation as a mechanism of substrate discrimination by adenylosuccinate synthetases.

    abstract::Adenylosuccinate synthetase catalyzes the first committed step in the de novo biosynthesis of AMP, coupling L-aspartate and IMP to form adenylosuccinate. Km values of IMP and 2'-deoxy-IMP are nearly identical with each substrate supporting comparable maximal velocities. Nonetheless, the Km value for L-aspartate and th...

    journal_title:Biochemistry

    pub_type: 杂志文章

    doi:10.1021/bi0607498

    authors: Iancu CV,Zhou Y,Borza T,Fromm HJ,Honzatko RB

    更新日期:2006-09-26 00:00:00

  • Induced alpha-helix structure in the aryl hydrocarbon receptor transactivation domain modulates protein-protein interactions.

    abstract::The aryl hydrocarbon receptor (AhR) is an intracellular receptor protein that regulates gene transcription in response to both man-made and natural ligands. A modular transactivaton domain (TAD) has been mapped to the 304 C-terminal amino acids and consists of acidic, Q-rich, and P/S/T-rich subdomains. We have used st...

    journal_title:Biochemistry

    pub_type: 杂志文章

    doi:10.1021/bi0487701

    authors: Watt K,Jess TJ,Kelly SM,Price NC,McEwan IJ

    更新日期:2005-01-18 00:00:00

  • Further characterization of the subunits of the receptor with high affinity for immunoglobulin E.

    abstract::The alpha, beta, and gamma subunits of the receptor with high affinity for immunoglobulin E were isolated and their compositions assessed by direct amino acid analysis and by incorporation of radioactive precursors. The compositions show no unusual features other than a rather high content of tryptophan in the alpha c...

    journal_title:Biochemistry

    pub_type: 杂志文章

    doi:10.1021/bi00383a024

    authors: Alcaraz G,Kinet JP,Liu TY,Metzger H

    更新日期:1987-05-05 00:00:00

  • Nematode Sgt1-homologue D1054.3 binds open and closed conformations of Hsp90 via distinct binding sites.

    abstract::Heat shock protein 90 (Hsp90) is a highly conserved ATP-driven machine involved in client protein maturation, folding, and activation. The chaperone is supported by a set of cochaperones that confer client specificities. One of those proteins is the suppressor of G2 allele of skp1 (Sgt1), which participates together w...

    journal_title:Biochemistry

    pub_type: 杂志文章

    doi:10.1021/bi5000542

    authors: Eckl JM,Drazic A,Rutz DA,Richter K

    更新日期:2014-04-22 00:00:00

  • Bleaching kinetics of artificial visual pigments with modifications near the ring-polyene chain connection.

    abstract::Absorbance difference spectra were recorded at 20 degrees C from 30 ns to milliseconds after photolysis of lauryl maltoside suspensions of artificial visual pigments derived from 9-cis isomers of 5-ethylretinal, 8,16-methanoretinal (a 6-s-trans-bicyclic analogue), or 5-demethyl-8-methylretinal. In all three pigments, ...

    journal_title:Biochemistry

    pub_type: 杂志文章

    doi:10.1021/bi011461f

    authors: Szundi I,de Lera AR,Pazos Y,Alvarez R,Oliana M,Sheves M,Lewis JW,Kliger DS

    更新日期:2002-02-12 00:00:00

  • Isolation and identification of cysteinyl peptide labeled by 6- [( 4-bromo-2,3-dioxobutyl)thio]-6-deaminoadenosine 5'-diphosphate in the reduced diphosphopyridine nucleotide inhibitory site of glutamate dehydrogenase.

    abstract::6-[(4-Bromo-2,3-dioxobutyl)thio]-6-deaminoadenosine 5'-diphosphate (6-BDB-TADP) has been shown to react at the reduced diphosphopyridine nucleotide (DPNH) inhibitory site of bovine liver glutamate dehydrogenase with incorporation of 1 mol of reagent/mol of enzyme subunit [Batra, S. P., & Colman, R. F. (1984) Biochemis...

    journal_title:Biochemistry

    pub_type: 杂志文章

    doi:10.1021/bi00360a005

    authors: Batra SP,Colman RF

    更新日期:1986-06-17 00:00:00

  • Structural evidence that the methionyl aminopeptidase from Escherichia coli is a mononuclear metalloprotease.

    abstract::The Co and Fe K-edge extended X-ray absorption fine structure (EXAFS) spectra of the methionyl aminopeptidase from Escherichia coli (EcMetAP) have been recorded in the presence of 1 and 2 equiv of either Co(II) or Fe(II) (i.e., [Co(II)_(EcMetAP)], [Co(II)Co(II)(EcMetAP)], [Fe(II)_(EcMetAP)], and [Fe(II)Fe(II)(EcMetAP)...

    journal_title:Biochemistry

    pub_type: 杂志文章

    doi:10.1021/bi010837m

    authors: Cosper NJ,D'souza VM,Scott RA,Holz RC

    更新日期:2001-11-06 00:00:00