Abstract:
:The behavior of photosystem II (PSII) reactions was investigated under conditions of decreasing water content by the addition of increasing concentrations of ethylene glycol (EG). The photosynthetic activities were measured for PSII samples either directly in aqueous solutions of EG or in the standard buffer medium following EG treatment. Several effects on PSII arise upon exposure to EG. Below 50% EG there are no significant irreversible changes, although there is a slowing of the QA-reoxidation kinetics in the presence of EG. At concentrations of 50-70% EG, protein structural changes occur that include the release of the 16, 23, and 33 kDa extrinsic proteins and two of the catalytic Mn ions. For these samples, the capacity for O2 evolution is considerably reduced and the formation of donor side H2O2 is enhanced. In 60% EG, the nanosecond components in the rate of P680+ reduction are converted entirely to microsecond kinetics which upon return of the sample to the standard buffer medium are partially restored, indicating that EG has a reversible, solvent effect on the PSII donor side. At concentrations of EG > 70% chlorophyll fluorescence measurements reveal reversible increases in the FO level concomitant with the generation and disappearance of a 5 microseconds decay component in the P680+ reduction kinetics. This result may indicate a solvent-induced uncoupling of the light harvesting pigment bed from the reaction center complex. As the EG concentration is increased to 80-100%, there is an irreversible loss of the primary charge separation. The use of EG as a cryoprotectant and as a water-miscible organic solvent for PSII is discussed.
journal_name
Biochemistryjournal_title
Biochemistryauthors
Hillier W,Lukins P,Seibert M,Wydrzynski Tdoi
10.1021/bi961724jsubject
Has Abstractpub_date
1997-01-07 00:00:00pages
76-85issue
1eissn
0006-2960issn
1520-4995pii
bi961724jjournal_volume
36pub_type
杂志文章相关文献
BIOCHEMISTRY文献大全abstract::PDZ (acronym of the synapse-associated protein PSD-95/SAP90, the septate junction protein Discs-large, and the tight junction protein ZO-1) domains are abundant small globular protein interaction domains that mainly recognize the carboxyl termini of their target proteins. Detailed knowledge on PDZ domain binding speci...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi700954e
更新日期:2007-11-27 00:00:00
abstract::Osmoregulatory transporters stimulate bacterial growth by mediating osmoprotectant uptake in response to increasing osmotic pressure. The ProP protein of Escherichia coli transports proline and other osmoprotectants. Like LacY, ProP is a member of the major facilitator superfamily and a H(+)-solute symporter. ProP is ...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi101281f
更新日期:2010-10-19 00:00:00
abstract::Excitation energy transfer and trapping processes in an iron stress-induced supercomplex of photosystem I from the cyanobacterium Synechocystis sp. PCC6803 were studied by time-resolved absorption and fluorescence spectroscopy on femtosecond and picosecond time scales. The data provide evidence that the energy transfe...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi026987u
更新日期:2003-04-08 00:00:00
abstract::Bacimethrin-derived 2'-methoxythiamin pyrophosphate inhibits microbial growth by disrupting metabolic pathways dependent on thiamin-utilizing enzymes. This study describes the discovery of the bacimethrin biosynthetic gene cluster of Clostridium botulinum A ATCC 19397 and in vitro reconstitution of bacimethrin biosynt...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi500281a
更新日期:2014-04-15 00:00:00
abstract::G-Actin is a globular protein (Mr 42 300) known to have three cysteine residues that are at least partially exposed and chemically reactive (Cys-10, -284, and -374). When G-actin was reacted with 3-bromo-1,1,1-trifluoropropanone, three resolvable 19F resonances were observed in the 19F NMR spectrum. This fluorinated G...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00356a050
更新日期:1986-04-22 00:00:00
abstract::A specific, high-affinity binding site for ammodytoxin C in synaptic membranes from bovine cerebral cortex was detected and partially characterized. Equilibrium binding analysis revealed a single population of [125I]ammodytoxin C acceptors with the following binding parameters: Kd = 6.0 nM and Bmax = 5.7 pmol/mg membr...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00250a049
更新日期:1994-11-22 00:00:00
abstract::Tissue plasminogen activator-inhibitor complexes were purified from the conditioned medium of human umbilical vein endothelial cells by affinity chromatography followed by gel filtration. It was found that a single complex was isolated which can exist in two distinct interconvertible conformations. These may be separa...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00397a037
更新日期:1987-11-17 00:00:00
abstract::Notwithstanding the relevance of their biological function, slow motions in proteins, beyond the microsecond range, are still poorly understood and often elusive. We propose that acrylamide quenching of Trp phosphorescence of deeply buried residues, when extended over the entire accessible range of lifetime measuremen...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi9009659
更新日期:2009-08-11 00:00:00
abstract::Folding experiments have suggested that some proteins have kinetic intermediates with a non-native structure. A simple G ̅o model does not explain such non-native intermediates. Therefore, the folding energy landscape of proteins with non-native intermediates should have characteristic properties. To identify such pro...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi200241a
更新日期:2011-07-26 00:00:00
abstract::Cytochrome P-450(M-1) [P-450(M-1)] is specifically expressed in adult male rat liver [Yoshioka, H., Morohashi, K., Sogawa, K., Miyata, T., Kawajiri, K., Hirose, T., Inayama, S., Fujii-Kuriyama, Y., & Omura, T. (1987) J. Biol. Chem. 262, 1706-1711]. Isolation and analysis of the gene for P-450(M-1) revealed that the co...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00399a039
更新日期:1987-12-15 00:00:00
abstract::Recent experiments [Wells, J., & Yount, R. (1979) Proc. Natl. Acad. Sci. U.S.A. 76, 4966] have shown it is possible to trap MgADP and other nucleotides stably at the active site of myosin by cross-linking two thiol groups. A variety of cross-linking reagents including chelation of the two thiols by cobalt (III) phenan...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00271a036
更新日期:1983-01-18 00:00:00
abstract::Treatment with cholesterol oxidases has shown that cholesterol is heterogeneously distributed in brush border membranes isolated from the apical domain of the renal and intestinal epithelial cells [Bloj, B., & Zilversmit, D. B. (1982) J. Biol. Chem. 257, 7608-7614; El Yandouzi, E. H., & Le Grimellec, C. (1992) Biochem...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00174a046
更新日期:1994-03-01 00:00:00
abstract::Light-driven outward sodium-pumping rhodopsin (NaR) was recently found in marine bacteria. Krokinobacter eikastus rhodopsin 2 (KR2) actively transports sodium and lithium ions in NaCl and LiCl, respectively, while it pumps protons in KCl. NaR has a conserved NDQ (N112, D116, and Q123 in KR2) motif, and previous studie...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/acs.biochem.6b00741
更新日期:2016-10-18 00:00:00
abstract::Alpha-synuclein plays a key role in the pathogenesis of many neurodegenerative diseases. To date, its cellular role has yet to be determined, although it has been proposed to be connected to calcium and G protein-mediated dopamine signaling. Alpha-synuclein is known to bind strongly to model membrane surfaces where it...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi0487140
更新日期:2005-01-18 00:00:00
abstract::Glycine oxidase gene from a strain of Bacillus subtilis was cloned and expressed in Escherichia coli. The purified enzyme was found, by mass spectrometry, to have a protein M(r) of 40763 (value of 40761.6 predicted from DNA sequence) and a FAD prosthetic group M(r) of 785.1 (theoretical value of 785.5). Glycine oxidas...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi100553n
更新日期:2010-08-31 00:00:00
abstract::Purine repressor (PurR) binding to specific DNA is enhanced by complexing with purines, whereas lactose repressor (LacI) binding is diminished by interaction with inducer sugars despite 30% identity in their protein sequences and highly homologous tertiary structures. Nonetheless, in switching from low- to high-affini...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi0028643
更新日期:2001-07-10 00:00:00
abstract::Product distributions for the reaction of glycolaldehyde labeled with carbon-13 at the carbonyl carbon ([1-(13)C]-GA) catalyzed by triosephosphate isomerase (TIM) in D(2)O at pD 7.0 in the presence of phosphite dianion and in its absence were determined by (1)H NMR spectroscopy. We observe three products for the relat...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi900636c
更新日期:2009-06-23 00:00:00
abstract::ATP, its nonhydrolyzable analogue, AMP-PNP, and albumin were found to promote the dissociation of rhodopsin kinase from rod outer segments (ROS) containing photoactivated-rhodopsin (Rho*). These features were embodied in a protocol for the recovery of rhodopsin kinase from incubations containing ROS which had been sub...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi952480q
更新日期:1996-05-14 00:00:00
abstract::To better elucidate RNA structure-function relationships and to improve the design of pharmaceutical agents that target specific RNA motifs, an understanding of RNA primary, secondary, and tertiary structure is necessary. The prediction of RNA secondary structure from sequence is an intermediate step in predicting RNA...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi3001227
更新日期:2012-07-03 00:00:00
abstract::Factor IX activation by factor XIa is thought to proceed through the singly-cleaved free intermediate, factor IX alpha. However, we observed no intermediate development during factor IX activation by factor XIa when using a low substrate to enzyme ratio (44:1 mol/mol). This result can be explained by one of two mechan...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi962274y
更新日期:1997-04-08 00:00:00
abstract::Proteins from the enhanced intracellular survival (Eis) family are versatile acetyltransferases that acetylate amines at multiple positions of several aminoglycosides (AGs). Their upregulation confers drug resistance. Homologues of Eis are present in diverse bacteria, including many pathogens. Eis from Mycobacterium t...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/acs.biochem.5b00244
更新日期:2015-05-26 00:00:00
abstract::Nramp (natural resistance-associated macrophage protein) family members have been characterized in mammals, yeast, and bacteria as divalent metal ion/H(+) symporters. In previous work, a bioinformatic approach was used for the identification of residues that are conserved within the Nramp family [Haemig, H. A., and Br...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi100320y
更新日期:2010-06-08 00:00:00
abstract::The divalent cation requirement for partial reactions of the catalytic turnover cycle during interfacial catalysis by pig pancreatic phospholipase A2 (PLA2) is investigated. Results show that the specific role of calcium in all the events of the catalytic cycle at the active site is not shared by other divalent cation...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00076a024
更新日期:1993-06-29 00:00:00
abstract::The dynamic formation of stress granules (SGs), processing bodies (PBs), and related RNA organelles regulates diverse cellular processes, including the coordination of functionally connected messengers, the translational regulation at the synapse, and the control of viruses and retrotransposons. Recent studies have sh...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/acs.biochem.8b00025
更新日期:2018-05-01 00:00:00
abstract::Rabbit aortic smooth muscle cells were evaluated for their ability to synthesize and accumulate glycosaminoglycans (GAGs). Because of the sensitivity of the microtechniques utilized, it is possible to determine the specific radioactivity of the GAGs obtained after radioactive incorporation of [35S]SO4(2-) and [14C]glu...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00550a026
更新日期:1980-04-29 00:00:00
abstract::In this study, we have mapped the intracellular alkylation sites of adozelesin and bizelesin, two potent analogs of CC-1065, in individual genes at the single-nucleotide level. Human colon carcinoma cells were treated with adozelesin and bizelesin, and the position of adducts were mapped within the PGK-1 and p53 genes...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00185a043
更新日期:1994-05-17 00:00:00
abstract::We propose a rapid chemical strategy for identifying base pairs in structured nucleic acid systems. The approach goes beyond traditional chemical mapping approaches by monitoring perturbations of each residue's chemical accessibility in response to systematic mutagenesis of residues that are distant in sequence but ne...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi101123g
更新日期:2010-09-07 00:00:00
abstract::The specificity of trypsin for Arg- and Lys-containing substrates depends upon the presence of Asp189 at the base of the primary binding pocket. The crystal structure of anionic rat trypsin D189S complexed with BPTI reveals that removal of the aspartate side chain permits the binding of a well-ordered acetate ion in a...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00177a016
更新日期:1994-03-22 00:00:00
abstract::[1-Penicillamine,2-leucine]oxytocin is a conformationally restricted analogue of oxytoxin in which the half-cystine-1 and tyrosine-2 residues of the native hormone are replaced by half-penicillamine (beta, beta-dimethyl-half-cystine) and leucine, respectively. This analogue is a surprisingly potent oxytocin antagonist...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00513a018
更新日期:1981-05-12 00:00:00
abstract::Extensive studies of the physiological protein-protein electron-transfer (ET) complex between yeast cytochrome c peroxidase (CcP) and cytochrome c (Cc) have left unresolved questions about how formation and dissociation of binary and ternary complexes influence ET. We probe this issue through a study of the photocycle...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi500888y
更新日期:2015-02-10 00:00:00