Cofactor Complexes of DesD, a Model Enzyme in the Virulence-related NIS Synthetase Family.

abstract：:The crystal structures of the guanosine 5'-monophosphate (GMP) and inosine 5'-monophosphate (IMP) complexes of Toxoplasma gondii hypoxanthine-guanine phosphoribosyltransferase (HGPRT) have been determined at 1.65 and 1.90 A resolution. These complexes, which crystallize in space groups P2(1) (a = 65.45 A, b = 90.84 A,...

journal_title：Biochemistry

authors： Héroux A,White EL,Ross LJ,Borhani DW

更新日期：1999-11-02 00:00:00

abstract：:Escherichia coli cAMP receptor protein (CRP) controls more than 20 genes. There are significant differences in the promoter regions in these genes. Thus, an elucidation of the mechanism of CRP action requires knowledge about the mode of selectivity in these promoters. An earlier study [Heyduk, T., & Lee, J. C. (1990) ...

journal_title：Biochemistry

authors： Pyles EA,Lee JC

更新日期：1996-01-30 00:00:00

abstract：:The hydration of CO2 catalyzed by carbonic anhydrase requires proton transfer from the zinc-bound water at the active site to solution for each cycle of catalysis. In the most efficient of the mammalian carbonic anhydrases, isozyme II, this transfer is facilitated by a proton shuttle residue, His 64. Murine carbonic a...

journal_title：Biochemistry

authors： Earnhardt JN,Qian M,Tu C,Laipis PJ,Silverman DN

更新日期：1998-05-19 00:00:00

abstract：:Oligonucleotide-directed mutagenesis in vivo was used to create destabilizing site specific changes at position 6 in iso-1-cytochrome c in Saccharomyces cerevisiae. These changes were made in combination with the stabilizing N52I substitution. The resulting proteins showed that a variety of forces and interactions are...

journal_title：Biochemistry

authors： Linske-O'Connell LI,Sherman F,McLendon G

更新日期：1995-05-30 00:00:00

abstract：:GTPases are key players during cellular signaling. Phosphorylation of Rab proteins, which belong to the Ras superfamily of small GTPases regulating intracellular transport, has been implicated in the pathogenesis of Parkinson's disease. For Rab8a, it was shown that serine 111 phosphorylation (pS111) is dependent on th...

journal_title：Biochemistry

authors： Pourjafar-Dehkordi D,Vieweg S,Itzen A,Zacharias M

更新日期：2019-08-20 00:00:00

abstract：:Exposure of mammalian cells (transformed mouse fibroblasts or rat hepatoma cells) to S-adenosyl-1,8-diamino-3-thiooctane produced profound changes in the intracellular polyamine content. Putrescine was increased and spermidine was decreased, consistent with the inhibition of spermidine synthase by this compound, which...

journal_title：Biochemistry

authors： Pegg AE,Tang KC,Coward JK

更新日期：1982-09-28 00:00:00

abstract：:Many biological processes are now known to be regulated by Ca2+ via calmodulin (CM). Although a general mechanistic model by which Ca2+ and calmodulin modulate many of these activities has been proposed, an accurate quantitative model is not available. A detailed analysis of skeletal muscle myosin light chain kinase a...

journal_title：Biochemistry

authors： Blumenthal DK,Stull JT

更新日期：1980-11-25 00:00:00

abstract：:Bovine milk galactosyltransferase was examined by ESR and NMR proton relaxation measurements to determine the stoichiometry and nature of manganese and UDP-Gal substrate binding. The ESR and NMR data clearly showed the binding of two (Mn(II) per mol of enzyme in the ternary complex (enzyme-manganese-UDP-Gal). The affi...

journal_title：Biochemistry

authors： Andree PJ,Berliner LJ

更新日期：1980-03-04 00:00:00

abstract：:Mutagenized dockerin domains of endoglucanase CelD (type I) and of the cellulosome-integrating protein CipA (type II) were constructed by swapping residues 10 and 11 of the first or the second duplicated segment between the two polypeptides. These residues have been proposed to determine the specificity of cohesin-doc...

journal_title：Biochemistry

authors： Schaeffer F,Matuschek M,Guglielmi G,Miras I,Alzari PM,Béguin P

更新日期：2002-02-19 00:00:00

abstract：:Numerous studies have been published stressing the importance of finding ligands that can bind specifically to DNA secondary structures. Several have identified ligands that are presented as having specific binding to the G-quadruplex; however, these were not originally tested on the complementary i-motif structure. T...

journal_title：Biochemistry

authors： Abdelhamid MAS,Gates AJ,Waller ZAE

更新日期：2019-01-29 00:00:00

abstract：:myo-Inositol oxygenase (MIOX) uses iron as its cofactor and dioxygen as its cosubstrate to effect the unique, ring-cleaving, four-electron oxidation of its cyclohexan-(1,2,3,4,5,6-hexa)-ol substrate to d-glucuronate. The nature of the iron cofactor and its interaction with the substrate, myo-inositol (MI), have been p...

journal_title：Biochemistry

authors： Xing G,Hoffart LM,Diao Y,Prabhu KS,Arner RJ,Reddy CC,Krebs C,Bollinger JM Jr

更新日期：2006-05-02 00:00:00

abstract：:A complex derived from chromatin containing one molecule of each of histones H2A, H2B, H3, and H4, termed core protein, was studied by 13C and 1H nuclear magnetic resonance. 13C line widths, when analyzed and compared with those of native and thermally unfolded representative globular proteins, showed that regions of ...

journal_title：Biochemistry

authors： Lilley DM,Pardon JF,Richards BM

更新日期：1977-06-28 00:00:00

abstract：:CYP2C19 is selective for the 4'-hydroxylation of S-mephenytoin while the highly similar CYP2C9 has little activity toward this substrate. To identify critical amino acids determining the specificity of human CYP2C19 for S-mephenytoin 4'-hydroxylation, we constructed chimeras by replacing portions of CYP2C9 containing ...

journal_title：Biochemistry

authors： Tsao CC,Wester MR,Ghanayem B,Coulter SJ,Chanas B,Johnson EF,Goldstein JA

更新日期：2001-02-20 00:00:00

abstract：:Azotobacter vinelandii bacterioferritin (AvBF) containing 800-1500 Co or Mn atoms as Co(III) and Mn(III) oxyhydroxide cores (Co-AvBF, Mn-AvBF) was synthesized by the same procedure used previously for horse spleen ferritin (HoSF). The kinetics of reduction of Co-AvBF and Mn-AvBF by ascorbic acid are first-order in eac...

journal_title：Biochemistry

authors： Zhang B,Harb JN,Davis RC,Choi S,Kim JW,Miller T,Chu SH,Watt GD

更新日期：2006-05-09 00:00:00

abstract：:A high-molecular-weight glycoprotein has been purified from the cervical mucus of the bonnet monkey (Macaca radiata). The glycoprotein was shown to be homogeneous by electrophoresis, sedimentation equilibrium, and N-terminal group determination, and to contain 19% protein, 19% D-galactose, 18% N-acetyl-D-galactosamine...

journal_title：Biochemistry

authors： Hatcher VB,Schwarzmann GO,Jeanloz RW,McArthur JW

更新日期：1977-04-05 00:00:00

abstract：:After borohydride reduction, carboxymethylation, and tryptic digestion of the holoenzyme of pig heart alanine aminotransferase, a single icosapeptide containing the N6-(phosphopyridoxyl)lysine residue was isolated by a combination of gel filtration and ion-exchange chromatogrpahy. Its primary structure was determined ...

journal_title：Biochemistry

authors： Tanase S,Kojima H,Morino Y

更新日期：1979-07-10 00:00:00

abstract：:O-Aryloxycarbonyl hydroxamates have previously been shown to efficiently inactivate class C β-lactamases by cross-linking serine and lysine residues in the active site. A new analogue of these inhibitors, D-(R)-O-(phenoxycarbonyl)-N-[(4-amino-4-carboxy-1-butyl)oxycarbonyl]hydroxylamine, designed to inactivate certain ...

journal_title：Biochemistry

authors： Tilvawala R,Cammarata M,Adediran SA,Brodbelt JS,Pratt RF

更新日期：2015-12-22 00:00:00

abstract：:Ionizing radiation, free radicals, and reactive oxygen species produce hundreds of different DNA lesions. Clustered lesions are typical for ionizing radiation. They compromise the efficiency of the base excision repair (BER) pathway, and as a consequence, they are much more toxic and mutagenic than isolated lesions. D...

journal_title：Biochemistry

authors： Zálešák J,Constant JF,Jourdan M

更新日期：2016-07-19 00:00:00

abstract：:Phosphorylase kinase, a regulatory enzyme of glycogenolysis in skeletal muscle, is a hexadecameric oligomer consisting of four copies each of a catalytic subunit (gamma) and three regulatory subunits (alpha, beta, and delta, the last being endogenous calmodulin). The enzyme is activated by a variety of effectors actin...

journal_title：Biochemistry

authors： Nadeau OW,Traxler KW,Fee LR,Baldwin BA,Carlson GM

更新日期：1999-02-23 00:00:00

abstract：:Type IIS restriction endonuclease Eco31I harbors a single HNH active site and cleaves both DNA strands close to its recognition sequence, 5'-GGTCTC(1/5). A two-domain organization of Eco31I was determined by limited proteolysis. Analysis of proteolytic fragments revealed that the N-terminal domain of Eco31I is respons...

journal_title：Biochemistry

authors： Jakubauskas A,Sasnauskas G,Giedriene J,Janulaitis A

更新日期：2008-08-19 00:00:00

abstract：:The sequence of two overlapping cDNA clones for the zinc metalloproteinase hemorrhagic toxin e (also known as atrolysin e, EC 3.4.24.44) from the venom gland of Crotalus atrox, the Western diamondback rattlesnake, is presented. The assembled cDNA sequence is 1975 nucleotides in length and encodes an open reading frame...

journal_title：Biochemistry

authors： Hite LA,Shannon JD,Bjarnason JB,Fox JW

更新日期：1992-07-14 00:00:00

abstract：:The binding to rat brain synaptosomes of a beta-scorpion toxin, i.e., toxin II of Centruroides suffusus suffusus (Css II), was studied as a function of pH, temperature, and concentration of some monovalent and divalent cations. At 10 degrees C and pH 6.0, the specific binding of 125I-labeled Css II corresponds to a si...

journal_title：Biochemistry

authors： Jover E,Bablito J,Couraud F

更新日期：1984-03-13 00:00:00

abstract：:Translational initiation factor IF-2 is involved in a multistep pathway leading to the synthesis of the first peptide bond. IF-2 is a guanine nucleotide binding protein (G-protein) and catalyzes GTP hydrolysis in the presence of ribosomes. According to sequence homologies with other G-proteins, particularly EF-Tu, a t...

journal_title：Biochemistry

authors： Vachon G,Laalami S,Grunberg-Manago M,Julien R,Cenatiempo Y

更新日期：1990-10-16 00:00:00

abstract：:Opossum (Didelphis virginiana) serum was fractionated with (NH4)2SO4 and then chromatographed on DEAE-Sepharose and phenyl-Sepharose. Affinity chromatography on a protein A-Sepharose-antibody column removed traces of opossum serum metalloproteinase inhibitors, and resulted in a homogeneous preparation of opossum alpha...

journal_title：Biochemistry

authors： Catanese JJ,Kress LF

更新日期：1993-01-19 00:00:00

abstract：:The trans-aconitate methyltransferase from the bacterium Escherichia coli catalyzes the monomethyl esterification of trans-aconitate and related compounds. Using two-dimensional (1)H/(13)C nuclear magnetic resonance spectroscopy, we show that the methylation is specific to one of the three carboxyl groups and further ...

journal_title：Biochemistry

authors： Cai H,Strouse J,Dumlao D,Jung ME,Clarke S

更新日期：2001-02-20 00:00:00

abstract：:Mouse epidermal growth factor (mEGF), a protein hormone effector molecule that regulates cellular development and division, has been investigated by using proton nuclear magnetic resonance techniques at 500 MHz. Well-resolved downfield aromatic and alpha-CH proton resonances (5.0-8.0 ppm) and an upfield ring current s...

journal_title：Biochemistry

authors： Mayo KH

更新日期：1984-08-14 00:00:00

abstract：:The AT-rich interaction domain (ARID) containing BAF250a is a subunit of the BAF-A class of SWI/SNF chromatin remodeling complexes. The ARID belongs to a family of conserved DNA binding domains found in several eukaryotic proteins; however, its exact contribution to BAF250a function and the mechanism of its DNA bindin...

journal_title：Biochemistry

authors： Giri M,Maulik A,Singh M

更新日期：2020-01-14 00:00:00

abstract：:Pulsed electron paramagnetic resonance spectroscopy has been used to detect Mn(II)-ligand superhyperfine couplings in complexes with creatine kinase and in the Mn(II) metalloprotein concanavalin A. Electron spin-echo envelopes from Mn(II), bound in these complexes, are modulated by superhyperfine interactions between ...

journal_title：Biochemistry

authors： LoBrutto R,Smithers GW,Reed GH,Orme-Johnson WH,Tan SL,Leigh JS Jr

更新日期：1986-09-23 00:00:00

abstract：:The properties of the homogeneous cutinase I, cutinase II, and the nonspecific esterase isolated from the extracellular fluid of cutin-grown Fusarium solani F. pisi (R.E. Purdy and P.E. Kolattukudy (1975), Biochemistry, preceding paper in this issue) were investigated. Using tritiated apple cutin as substrate, the two...

journal_title：Biochemistry

authors： Purdy RE,Kolattukudy PE

更新日期：1975-07-01 00:00:00

abstract：:The binding of horse ferricytochrome c to yeast cytochrome c peroxidase at pH 6.0 in 8.7 mM phosphate buffer (0.0100 M ionic strength) is characterized by a small, unfavorable enthalpy change (+1.91 +/- 0.16 kcal mol-1) and a large, positive entropy change (+37 +/- 1 eu). The free energy of binding depends strongly up...

journal_title：Biochemistry

authors： Kresheck GC,Vitello LB,Erman JE

更新日期：1995-07-04 00:00:00