Abstract：
:Human immunodeficiency virus type 1 (HIV-1) Vpu is an integral membrane protein that belongs to the viroporin family. Viroporins interact with cell membranes, triggering membrane permeabilization and promoting release of viral particles. In vitro electrophysiological methods have revealed changes in membrane ion currents when Vpu is present; however, in vivo the molecular mechanism of Vpu at the plasma membrane is still uncertain. We used the yeast Saccharomyces cerevisiae as a genetic model system to analyze how Vpu ion channel impacts cellular homeostasis. Inducible expression of Vpu impaired cell growth, suggesting that this viral protein is toxic to yeast cultures. This toxicity decreased with extracellular acidic pH. Also, Vpu toxicity diminished as the extracellular K(+) concentration was increased. However, expression of the Vpu protein suppresses the growth defect of K(+) uptake-deficient yeast (Δtrk1,2). The phenotype rescue of these highly hyperpolarized cells was almost total when they were grown in medium supplemented with high concentrations of KCl (100 mM) at pH 7.0 but was significantly reduced when the extracellular K(+) concentration or pH was decreased. These results indicate that Vpu has the ability to modify K(+) transport in both yeast strains. Here, we show also that Vpu confers tolerance to the aminoglycoside antibiotic hygromycin B in Δtrk1,2 yeast. Our results suggest that Vpu interferes with cell growth of wild-type yeast but improves proliferation of the hyperpolarized trk1,2 mutant by inducing plasma membrane depolarization. Furthermore, evaluation of the ion channel activity of the Vpu protein in Δtrk1,2 yeast could aid in the development of a high-throughput screening assay for molecules that target the retroviral protein.
journal_name
Biochemistryjournal_title
Biochemistryauthors
Herrero L,Monroy N,González MEdoi
10.1021/bi3011175subject
Has Abstractpub_date
2013-01-08 00:00:00pages
171-7issue
1eissn
0006-2960issn
1520-4995journal_volume
52pub_type
杂志文章相关文献
BIOCHEMISTRY文献大全abstract：:Although the terms "excessive reactive oxygen species (ROS)" and "oxidative stress" are widely used, the implications of oxidative stress are often misunderstood. ROS are not a single species but a variety of compounds, each with unique biochemical properties and abilities to react with biomolecules. ROS cause activat...
journal_title：Biochemistry
pub_type： 杂志文章,评审
doi：10.1021/acs.biochem.6b00703
更新日期：2016-09-27 00:00:00
abstract：:DNA topoisomerase II is believed to be the enzyme that produces the protein-associated DNA strand breaks observed in mammalian cell nuclei treated with various intercalating agents. Two intercalators--4'-(9-acridinylamino)methanesulfon-m-anisidide (m-AMSA, amsacrine) and 2-methyl-9-hydroxyellipticinium (2-Me-9-OH-E+)-...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00344a015
更新日期：1985-11-05 00:00:00
abstract：:The subtilisins have an extended substrate binding cleft comprising at least 8 subsites. Two pockets at the S1 and S4 sites are particularly conspicuous, and the interactions between substrate and these two pockets are very important for the substrate specificity. Phe residues have mutationally been introduced at one ...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00062a016
更新日期：1993-03-23 00:00:00
abstract：:Although the mechanism by which the steroidogenic acute regulatory protein (StAR) promotes steroidogenesis has been studied extensively, it remains incompletely characterized. Because structural analysis has revealed a hydrophobic sterol-binding pocket (SBP) within StAR, this study sought to examine the regulatory rol...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi401125v
更新日期：2013-10-15 00:00:00
abstract：:Isotope effects have been measured with Mg2+ as the activator and L-malate labeled with deuterium or tritium at carbon 2 as the substrate over the pH range 4-10. Comparison of the nearly pH-independent deuterium-isotope effect on V/Kmalate of 1.5 with the tritium effect of 2.0 by the method of Northrop (Northrop, D.B....
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00623a002
更新日期：1977-02-22 00:00:00
abstract：:Pulmonary surfactant protein D (SP-D), a member of the collectin group of innate immune proteins, plays important roles in lipopolysaccharide (LPS) recognition. We have previously shown that surfactant protein A (SP-A), a homologous collectin, interacts with Toll-like receptor (TLR) 2, resulting in alteration of TLR2-...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi060176z
更新日期：2006-07-18 00:00:00
abstract：:Protein-protein interactions such as those between small G proteins and their effector proteins control most cell signaling pathways and thereby govern many cellular processes in both normal and disease states. Each small G protein interacts with several effectors, some shared between similar G proteins and others uni...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi701539x
更新日期：2007-12-11 00:00:00
abstract：:d-2-Hydroxyglutarate dehydrogenase from Pseudomonas aeruginosa PAO1 (PaD2HGDH) catalyzes the oxidation of d-2-hydroxyglutarate to 2-ketoglutarate, which is a necessary step in the serine biosynthetic pathway. The dependence of P. aeruginosa on PaD2HGDH makes the enzyme a potential therapeutic target against P. aerugin...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/acs.biochem.0c00832
更新日期：2020-12-29 00:00:00
abstract：:Protein phosphatase 1 (PP1) is an essential and ubiquitous serine/threonine protein phosphatase that is regulated by more than 100 known inhibitor and targeting proteins. It is currently unclear how protein inhibitors distinctly and specifically regulate PP1 to enable rapid responses to cellular alterations. We demons...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi801308y
更新日期：2008-11-25 00:00:00
abstract：:R67 dihydrofolate reductase (DHFR), which catalyzes the NADPH dependent reduction of dihydrofolate to tetrahydrofolate, belongs to a type II family of R-plasmid encoded DHFRs that confer resistance to the antibacterial drug trimethoprim. Crystal structure data reveals this enzyme is a homotetramer that possesses a sin...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi034643d
更新日期：2003-09-16 00:00:00
abstract：:The Bacillus subtilis DNA-dependent RNA polymerase holoenzyme and core enzyme each contain approximately two atoms of zinc per molecule. When the dissociated subunits of the enzyme are passed through a blue dextran-Sepharose affinity column, only the beta subunit binds to the column. The total zinc content of the enzy...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00632a012
更新日期：1977-06-28 00:00:00
abstract：:Zinc finger nucleases (ZFNs) have been used to direct precise modifications of the genetic information in living cells at high efficiency. An important consideration in the design of ZFNs is the number of zinc fingers that are required for efficient and specific cleavage. We examined dimeric ZFNs composed of [1]+[1], ...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi200393g
更新日期：2011-06-07 00:00:00
abstract：:High-resolution phosphorus-31 nuclear magnetic resonance (31P NMR) spectra of wild-type and mutant strains of Saccharomyces cerevisiae were observed at a frequency of 145.7 MHz. Levels of various phosphorus metabolites were investigated upon addition of glucose under both aerobic and anaerobic conditions. Three mutant...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00588a006
更新日期：1979-10-16 00:00:00
abstract：:Isopenicillin N synthase (IPNS) catalyzes the oxidative cyclization of delta-(L-alpha-aminoadipoyl)-L-cysteinyl-D-valine to isopenicillin N. It is proposed that the multiple products produced from certain substrate analogues result from pathway branching after formation of a ferryl oxene intermediate. We have been int...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00022a031
更新日期：1995-06-06 00:00:00
abstract：:The binding of bleomycin to DNA in the presence and absence of ferric iron was measured by fluorescence spectroscopy. In millimolar concentrations of tris(hydroxymethyl)aminomethane, pH 7.5, approximately 80% of the bleomycin binds to DNA. Ferric iron seems to have no significant effect on the binding of DNA to bleomy...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00531a021
更新日期：1982-01-19 00:00:00
abstract：:A steady-state kinetic analysis was conducted of the overall aminoacylation reaction catalyzed by isoleucyl-tRNA synthetase. The patterns of Lineweaver-Burk plots obtained indicated that tRNA adds to the enzyme only after isoleucyl adenylate formation and pyrophosphate release. These kinetic patterns were consistent w...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00580a018
更新日期：1979-06-26 00:00:00
abstract：:The alpha-helix formed by the amino acid residues 9-19 of the B-chain of insulin is involved in the stabilization of its three-dimensional structure. We have shown that modification at positions B9, B10, B12, and B16 results in analogues possessing biological activities ranging from ca. 0.2% to ca. 500% relative to th...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00061a022
更新日期：1993-03-16 00:00:00
abstract：:Gemcitabine 5'-diphosphate (F(2)CDP) is a potent inhibitor of ribonucleotide reductases (RNRs), enzymes that convert nucleotides (NDPs) to deoxynucleotides and are essential for DNA replication and repair. The Escherichia coli RNR, an alpha2beta2 complex, when incubated with 1 equiv of F(2)CDP catalyzes the release of...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi901590q
更新日期：2009-12-15 00:00:00
abstract：:Six active site mutants of Escherichia coli phosphofructokinase have been constructed and characterized using steady-state kinetics. All but one of the mutants (ES222) have significantly lower maximal activity, implicating these residues in the catalytic process. Replacement of Asp127, the key catalytic residue in the...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00153a017
更新日期：1992-09-29 00:00:00
abstract：:The solution structure of the actinorhodin acyl carrier protein (act apo-ACP) from the polyketide synthase (PKS) of Streptomyces coelicolor A3(2) has been determined using 1H NMR spectroscopy, representing the first polyketide synthase component for which detailed structural information has been obtained. Twenty-four ...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi970006+
更新日期：1997-05-20 00:00:00
abstract：:Horseradish peroxidase C binds a wide variety of small H-donor compounds such as benzohydroxamic acid (BHA) and 2-naphthohydroxamic acid (NHA). In this work, we use the Mg(II)-mesoporphyrin prosthetic group derivative as a spectroscopic probe of the active site and of the interaction with the substrates. We report on ...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi002938a
更新日期：2001-08-07 00:00:00
abstract：:Five gangliosides, accounting for over 95% of the total ganglioside fraction, were isolated from bovine adrenal medulla by preparative thin-layer chromatography and the carbohydrate structures determined by a combination of periodate oxidation and permethylation techniques. Partially methylated alditol acetates were g...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00678a026
更新日期：1975-04-08 00:00:00
abstract：:Although plasma membrane domains, such as caveolae, provide an organizing principle for signaling pathways and cholesterol homeostasis in the cell, relatively little is known regarding specific mechanisms, whereby intracellular lipid-binding proteins are targeted to caveolae. Therefore, the interaction between caveoli...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi7002636
更新日期：2007-07-17 00:00:00
abstract：:The Na+-translocating NADH:ubiquinone oxidoreductase (Na+-NQR) from Vibrio harveyi was purified and studied by EPR and visible spectroscopy. Two EPR signals in the NADH-reduced enzyme were detected: one, a radical signal, and the other a line around g = 1.94, which is typical for a [2Fe-2S] cluster. An E(m) of -267 mV...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi002545b
更新日期：2001-06-19 00:00:00
abstract：:We assigned l-talarate dehydratase (TalrD) and galactarate dehydratase (GalrD) functions to a group of orthologous proteins in the mechanistically diverse enolase superfamily, focusing our characterization on the protein encoded by the Salmonella typhimurium LT2 genome (GI:16766982; STM3697). Like the homologous mande...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi7008882
更新日期：2007-08-21 00:00:00
abstract：:A statistical mechanical partition function for phospholipid bilayers is constructed to obtain a theoretical description of the chain melting phase transition in lipid bilayer membranes and of the phase diagrams for two-component bilayers. In addition to providing an accurate representation of the transition temperatu...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00639a004
更新日期：1977-10-04 00:00:00
abstract：:Lytic polysaccharide monooxygenases (LPMOs) are ubiquitous oxidoreductases, facilitating the degradation of polymeric carbohydrates in biomass. Cellobiose dehydrogenase (CDH) is a biologically relevant electron donor in this process, with the electrons resulting from cellobiose oxidation being shuttled from the CDH de...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/acs.biochem.8b01178
更新日期：2019-03-05 00:00:00
abstract：:Type I repeat sequences are evolutionarily conserved sequence elements found in the replication origin and transcriptional promoter region of the rRNA genes (rDNA) in Tetrahymena thermophila. An abundant single-stranded DNA binding protein, ssA-TIBF, specifically interacts with the A-rich strand of the Type I repeat s...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00014a011
更新日期：1995-04-11 00:00:00
abstract：:Smooth muscle cells maintain filaments of actin and myosin in the presence of ATP, although dephosphorylated myosin filaments and actin-myosin interactions are unstable under those conditions in vitro. Several proteins that stabilize myosin filaments and that stabilize actin-myosin interactions have been identified. F...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi401013g
更新日期：2013-10-29 00:00:00
abstract：:The stereochemical problem involving a pro-pro-prochiral phosphorus center, the hydrolysis of adenosine 5'-monophosphate to adenosine and inorganic phosphate catalyzed by the venom 5'-nucleotidase, has been studied by use of chiral [16O, 17O, 18O]thiophosphates (Psi). (Rp)- and (Sp)-[alpha-18O1]Adenosine 5'-thiophosph...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00564a025
更新日期：1980-11-11 00:00:00
