Excessive Reactive Oxygen Species and Exotic DNA Lesions as an Exploitable Liability.

abstract：:The binding modes of four active site-directed, acylating inhibitors of human alpha-thrombin have been determined using X-ray crystallography. These inhibitors (GR157368, GR166081, GR167088, and GR179849) are representatives of a series utilizing a novel 5, 5-trans-lactone template to specifically acylate Ser195 of th...

journal_title：Biochemistry

authors： Jhoti H,Cleasby A,Reid S,Thomas PJ,Weir M,Wonacott A

更新日期：1999-06-22 00:00:00

abstract：:The nonconvertible substrate analogues di-, tri-, and tetramethylammonium are bound with fairly high affinity to oxidized methylamine dehydrogenase (MADHox) from Thiobacillus versutus and induce the same red-shift in the optical absorbance spectrum of MADHox as do the monovalent cations Cs+, Rb+, and NH4+. Like the mo...

journal_title：Biochemistry

authors： Gorren AC,Moenne-Loccoz P,Backes G,de Vries S,Sanders-Loehr J,Duine JA

更新日期：1995-10-10 00:00:00

abstract：:With knowledge of individual transfer free energies of chemical groups that become newly exposed on protein denaturation and assuming the group transfer free energy contributions are additive, it should be possible to predict the stability of a protein in the presence of denaturant. Unfortunately, several unresolved i...

journal_title：Biochemistry

authors： Auton M,Bolen DW

更新日期：2004-02-10 00:00:00

abstract：:We have used DNase I footprinting to examine the formation of antiparallel DNA triple helices on DNA fragments containing the homopurine target sites (GGA)2GGX(GGA)2GG.(CCT)2CCZ(CCT)2CC (where X.Z is each base pair in turn), with the GA- and GT-rich oligonucleotides, (GGA)2GGN(GGA)2GG and (GGT)2GGN(GGT)2GG (N = each b...

journal_title：Biochemistry

authors： Chandler SP,Fox KR

更新日期：1996-11-26 00:00:00

abstract：:We have studied the binding nature of an aromatic aldehyde to the catalytic site of liver alcohol dehydrogenase from horse (LADH) using preresonance Raman spectroscopy. The compound p-(dimethylamino)benzaldehyde (DABA) is converted to the corresponding alcohol in the presence of nicotinamide adenine dinucleotide (NADH...

journal_title：Biochemistry

authors： Callender R,Chen D,Lugtenburg J,Martin C,Rhee KW,Sloan D,Vandersteen R,Yue KT

更新日期：1988-05-17 00:00:00

abstract：:The interactions of the ligand binding domain (LBD) of androgen receptor (AR) and the AR T877A mutant, found in prostate cancer, with peptides from coactivator and corepressor proteins or random phage display peptides were investigated using in vitro time-resolved fluorescence resonance energy transfer (TR-FRET). Inte...

journal_title：Biochemistry

authors： Ozers MS,Marks BD,Gowda K,Kupcho KR,Ervin KM,De Rosier T,Qadir N,Eliason HC,Riddle SM,Shekhani MS

更新日期：2007-01-23 00:00:00

abstract：:Site-directed mutants were prepared of four consecutive and highly conserved residues (His-411, Asp-412, Thr-413, Tyr-414) of an extramembrane loop that connects putative transmembrane helices IX and X of subunit I of Rhodobacter sphaeroides cytochrome c oxidase. The modified enzymes were purified and analyzed by opti...

journal_title：Biochemistry

authors： Hosler JP,Shapleigh JP,Tecklenburg MJ,Thomas JW,Kim Y,Espe M,Fetter J,Babcock GT,Alben JO,Gennis RB

更新日期：1994-02-08 00:00:00

abstract：:Members of the ATP-binding cassette (ABC) transporters share the same basic architecture, with a four-core domain made of two transmembrane plus two nucleotide-binding domains. However, a supramolecular organization has been detected in some ABC transporters, which might be relevant to physiological regulation of subs...

journal_title：Biochemistry

authors： Dalmas O,Do Cao MA,Lugo MR,Sharom FJ,Di Pietro A,Jault JM

更新日期：2005-03-22 00:00:00

abstract：:Factors determining conformations of imidazole axially coordinated to heme in heme proteins were investigated by analyzing 693 hemes in 432 different crystal structures of heme proteins from the Protein Data Bank (PDB), where at least one histidine is ligated to heme. The results from a search of the PDB for protein s...

journal_title：Biochemistry

authors： Zarić SD,Popović DM,Knapp EW

更新日期：2001-07-03 00:00:00

abstract：:The hydrogen exchange behavior of the N-terminal helical segment in horse heart cytochrome c was studied in both the reduced and the oxidized forms by use of two-dimensional nuclear magnetic resonance methods. The amide protons of the first six residues are not H bonded and exchange rapidly with solvent protons. The m...

journal_title：Biochemistry

authors： Wand AJ,Roder H,Englander SW

更新日期：1986-03-11 00:00:00

abstract：:Human hemoglobin produced in the Escherichia coli coexpression system of Hernan et al. [(1992) Biochemistry 31, 8619-8628] has been transformed into a functionally homogeneous protein whose properties closely approximate those of normal hemoglobin A. Both of the alpha and beta chains of this hemoglobin contain a valin...

journal_title：Biochemistry

authors： Hui HL,Kavanaugh JS,Doyle ML,Wierzba A,Rogers PH,Arnone A,Holt JM,Ackers GK,Noble RW

更新日期：1999-01-19 00:00:00

abstract：:Platelet receptor recognition domains are located on the gamma and alpha chains of human fibrinogen. The former encompasses residues 400-411 [Kloczewiak, M., Timmons, S., Lukas, T. J., & Hawiger, J. (1984) Biochemistry 23, 1767], and the latter is present in two loci on the alpha chain (alpha 95-97 and alpha 572-574) ...

journal_title：Biochemistry

authors： Timmons S,Bednarek MA,Kloczewiak M,Hawiger J

更新日期：1989-04-04 00:00:00

abstract：:Biochemical and immunological properties of biosynthetically radiolabeled phosphatidylcholine-(PC-) binding proteins were investigated. The PC-binding proteins were extracted from the detergent lysate of biosynthetically radiolabeled P388D1 cells by affinity chromatography on PC-Sepharose and filtered through a Sephad...

journal_title：Biochemistry

authors： Fernandez-Botran R,Suzuki T

更新日期：1985-04-09 00:00:00

abstract：:A metastable intermediate was found in the course of the denaturation of purified cytochrome P450 2B1 by increasing concentrations of guanidine hydrochloride (GuHCl). The metastable intermediate has no or low absorbance at 450 nm in the reduced carbon monoxide difference spectrum and has no absorbance at 420 nm. The i...

journal_title：Biochemistry

authors： Yu XC,Shen S,Strobel HW

更新日期：1995-04-25 00:00:00

abstract：:The self-association of rabbit muscle phosphofructokinase at pH 7.0 was investigated by velocity sedimentation. The process was demonstrated to be in a rapid, dynamic equilibrium. The concentration dependence of the weight-average sedimentation coefficient was monitored within the range of 10-750 microgram/mL. The sed...

journal_title：Biochemistry

authors： Hesterberg LK,Lee JC

更新日期：1981-05-12 00:00:00

abstract：:Extracellular matrix (ECM) proteins, such as collagen and fibronectin, play vital roles in development and maintenance of hard tissue (bone or tooth) and are, consequently, thoroughly investigated for construction of biomimetic scaffolds in combination with calcium phosphate (CaP) material (the major component of hard...

journal_title：Biochemistry

authors： Chowdhury EH,Nagaoka M,Ogiwara K,Zohra FT,Kutsuzawa K,Tada S,Kitamura C,Akaike T

更新日期：2005-09-20 00:00:00

abstract：:Type II topoisomerases are responsible for DNA unlinking during DNA replication and chromosome segregation. Although eukaryotic enzymes are homodimers and prokaryotic enzymes are heterotetramers, both prokaryotic and eukaryotic type II topoisomerases belong to a single protein family. The amino- and carboxyl-terminal ...

journal_title：Biochemistry

authors： Lavasani LS,Hiasa H

更新日期：2001-07-24 00:00:00

abstract：:Protein folding in vivo takes place in a highly crowded environment. The resulting excluded volume forces are thought to stabilize folded forms of proteins. In agreement, many in vitro studies have shown that the presence of macromolecular crowding agents increases the stability of folded proteins but often by only a ...

journal_title：Biochemistry

authors： Adén J,Wittung-Stafshede P

更新日期：2014-04-15 00:00:00

abstract：:PACT is a stress-modulated activator of interferon (IFN)-induced double-stranded (ds) RNA-activated protein kinase (PKR) and is an important regulator of PKR-dependent signaling pathways. Stress-induced phosphorylation of PACT is essential for PACT's association with PKR leading to PKR activation. PKR activation by PA...

journal_title：Biochemistry

authors： Singh M,Castillo D,Patel CV,Patel RC

更新日期：2011-05-31 00:00:00

abstract：:Combined quantum mechanical/molecular mechanical (QM/MM) simulations are carried out to analyze factors that dictate the proton transfer in carbonic anhydrase II (CAII), an enzyme that has been used as a prototypical example of long-range proton transfers in biomolecules. In contrast to the long-held conjecture in the...

journal_title：Biochemistry

authors： Riccardi D,König P,Guo H,Cui Q

更新日期：2008-02-26 00:00:00

abstract：:Protein methylation is a common post-translational modification with diverse biological functions. Methyllysine reader proteins are increasingly a focus of epigenetics research and play important roles in regulating many cellular processes. These reader proteins are vital players in development, cell cycle regulation,...

journal_title：Biochemistry

authors： Milosevich N,Hof F

更新日期：2016-03-22 00:00:00

abstract：:Human transcriptional coactivator P/CAF (p300/CBP-associating factor) is a histone acetyltransferase (HAT) and is a member of the GNAT (GCN5 related N-acetyltransferases) superfamily. P/CAF was originally identified by its ability to activate transcription of a variety of genes through its interaction with p300/CBP. T...

journal_title：Biochemistry

authors： Tanner KG,Langer MR,Denu JM

更新日期：2000-10-03 00:00:00

abstract：:The importance of DNA polymerase-DNA minor groove interactions on translesion synthesis (TLS) was examined in vitro using variants of exonuclease-deficient Klenow polymerase and site-specifically modified DNA oligonucleotides. Polymerase variant R668A lacks primer strand interactions, while variant Q849A lacks templat...

journal_title：Biochemistry

authors： Gestl EE,Eckert KA

更新日期：2005-05-10 00:00:00

abstract：:The WSXWS motif is a highly conserved structural feature of the type I cytokine receptor family. It has previously been demonstrated that mutations in the (232)WSAWS(236) motif in the erythropoietin receptor (EPOR) can result in strongly inhibited surface expression, due to defective intracellular transport [Hilton, D...

journal_title：Biochemistry

authors： Furmanek A,Hess D,Rogniaux H,Hofsteenge J

更新日期：2003-07-22 00:00:00

abstract：:The hinge domain encompasses amino acids 51-60 of lactose repressor (LacI) and plays an important role in its regulatory interaction with operator DNA. This segment makes both hinge-DNA and hinge-hinge' contacts that are critical to DNA binding. Furthermore, this small region serves as a central element in communicati...

journal_title：Biochemistry

authors： Falcon CM,Matthews KS

更新日期：2001-12-25 00:00:00

abstract：:Chymopapain, a cysteine protease of papaya latex, has been purified with the use of fast protein liquid chromatography. Two homogeneous fractions were analyzed for thiol content and thiol reactivity. It was found that peak 1 and peak 2 contained two and three thiol groups, respectively, per mole of enzyme. This result...

journal_title：Biochemistry

authors： Kóródi I,Asbóth B,Polgár L

更新日期：1986-11-04 00:00:00

abstract：:The equilibrium binding of hemoglobin to isolated band 3 protein exhibited positive cooperativity [Hill coefficient = 1.65 +/- 0.1; total number of binding sites at pH 6.6 in 5 mM sodium phosphate buffer = 32 500 +/- 940 pmol/mg; Ka = (3.0 +/- 0.5) X 10(5) M-1]. The binding was reversible and ionic in nature as the bo...

journal_title：Biochemistry

authors： Premachandra BR

更新日期：1986-06-03 00:00:00

abstract：:Isolation and characterization of a novel radiation-induced product, i.e., the 8-hydroxyguanine residue, produced in deoxyribonucleic acid (DNA), 2'-deoxyguanosine, and 2'-deoxyguanosine 5'-monophosphate by gamma-irradiation in aqueous solution, are described. For this purpose, gamma-irradiated DNA was first hydrolyze...

journal_title：Biochemistry

authors： Dizdaroglu M

更新日期：1985-07-30 00:00:00

abstract：:Cuprizone intoxication is a common animal model used to test myelin regenerative therapies for the treatment of diseases such as multiple sclerosis. Mice fed this copper chelator develop reversible, region-specific oligodendrocyte loss and demyelination. While the cellular changes influencing the demyelinating process...

journal_title：Biochemistry

authors： Taraboletti A,Walker T,Avila R,Huang H,Caporoso J,Manandhar E,Leeper TC,Modarelli DA,Medicetty S,Shriver LP

更新日期：2017-03-14 00:00:00

abstract：:The iron-sulfur flavoenzyme adenosine-5'-phosphosulfate (APS) reductase catalyzes a key reaction of the global sulfur cycle by reversibly transforming APS to sulfite and AMP. The structures of the dissimilatory enzyme from Archaeoglobus fulgidus in the reduced state (FAD(red)) and in the sulfite adduct state (FAD-sulf...

journal_title：Biochemistry

authors： Schiffer A,Fritz G,Kroneck PM,Ermler U

更新日期：2006-03-07 00:00:00