Abstract:
:The WSXWS motif is a highly conserved structural feature of the type I cytokine receptor family. It has previously been demonstrated that mutations in the (232)WSAWS(236) motif in the erythropoietin receptor (EPOR) can result in strongly inhibited surface expression, due to defective intracellular transport [Hilton, D. J., et al. (1996) J. Biol. Chem. 271, 4699-4708]. Here we report that the first tryptophan in the motif of the recombinant extracellular domain of EPOR (sEPOR) expressed in HEK-EBNA cells carries a C-linked hexosyl residue. The S233A mutation completely abolished secretion of sEPOR, whereas the A234E mutation resulted in enhanced secretion. Comparison of the level of C-hexosylation in the wild-type protein and in the mutant proteins isolated from the conditioned medium and/or the cells suggested that C-hexosylation of the motif did not play a role in the correct intracellular transport of sEPOR.
journal_name
Biochemistryjournal_title
Biochemistryauthors
Furmanek A,Hess D,Rogniaux H,Hofsteenge Jdoi
10.1021/bi034112psubject
Has Abstractpub_date
2003-07-22 00:00:00pages
8452-8issue
28eissn
0006-2960issn
1520-4995journal_volume
42pub_type
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