Photoinactivation of peptide transport in Saccharomyces cerevisiae.

abstract：:The equilibrium binding of hemoglobin to isolated band 3 protein exhibited positive cooperativity [Hill coefficient = 1.65 +/- 0.1; total number of binding sites at pH 6.6 in 5 mM sodium phosphate buffer = 32 500 +/- 940 pmol/mg; Ka = (3.0 +/- 0.5) X 10(5) M-1]. The binding was reversible and ionic in nature as the bo...

journal_title：Biochemistry

authors： Premachandra BR

更新日期：1986-06-03 00:00:00

abstract：:A comparison of the oxidations of diclofenac with microsomes of yeasts expressing various human liver cytochromes P450 showed that P450 2C9 regioselectively led to 4'-hydroxy diclofenac (4'-OHD) whereas P450 3A4 only led to 5-hydroxy diclofenac (5-OHD). P450 2C19, 2C18, and 2C8 led to the simultaneous formation of 4'-...

journal_title：Biochemistry

authors： Mancy A,Antignac M,Minoletti C,Dijols S,Mouries V,Duong NT,Battioni P,Dansette PM,Mansuy D

更新日期：1999-10-26 00:00:00

abstract：:The vitamin D receptor (VDR) binds 1,25-dihydroxyvitamin D(3) and mediates its actions on gene transcription by heterodimerizing with retinoid X receptors (RXRs) on direct repeat (DR+3) vitamin D responsive elements (VDREs) located in target genes. The VDRE binding function of VDR has been primarily ascribed to the zi...

journal_title：Biochemistry

authors： Hsieh JC,Whitfield GK,Oza AK,Dang HT,Price JN,Galligan MA,Jurutka PW,Thompson PD,Haussler CA,Haussler MR

更新日期：1999-12-07 00:00:00

abstract：:Proteorhodopsins are a recently discovered class of microbial rhodopsins, ubiquitous in marine bacteria. Over 4000 variants have thus far been discovered, distributed throughout the oceans of the world. Most variants fall into one of two major groups, green- or blue-absorbing proteorhodopsin (GPR and BPR, respectively...

journal_title：Biochemistry

authors： Kralj JM,Bergo VB,Amsden JJ,Spudich EN,Spudich JL,Rothschild KJ

更新日期：2008-03-18 00:00:00

abstract：:A novel inhibitor of p38 mitogen-activated protein kinase (p38), CMPD1, identified by high-throughput screening, is characterized herein. Unlike the p38 inhibitors described previously, this inhibitor is substrate selective and noncompetitive with ATP. In steady-state kinetics experiments, CMPD1 was observed to preven...

journal_title：Biochemistry

authors： Davidson W,Frego L,Peet GW,Kroe RR,Labadia ME,Lukas SM,Snow RJ,Jakes S,Grygon CA,Pargellis C,Werneburg BG

更新日期：2004-09-21 00:00:00

abstract：:Designing subtype-selective agonists for neuronal nicotinic acetylcholine receptors is a challenging and significant goal aided by intricate knowledge of each subtype's binding patterns. We previously reported that in α6β2 receptors, acetylcholine makes a functional cation-π interaction with Trp149, but nicotine and T...

journal_title：Biochemistry

authors： Post MR,Lester HA,Dougherty DA

更新日期：2017-04-04 00:00:00

abstract：:Thermal pertubation difference spectra of sperm whale myoglobin (Mb) and soybean leghemoglobin a (Lb a) in the near-ultraviolet reveal similarities in the tryptophan environment of the two proteins. Of the two tryptophans in each protein, one has its indolyl NH group fully exposed to aqueous solvent, while the other b...

journal_title：Biochemistry

authors： Nicola NA,Leach SJ

更新日期：1977-01-11 00:00:00

abstract：:The characterization of cod somatolactin (SL), a new pituitary protein belonging to the growth hormone/prolactin family, is described. Cod SL has a molecular weight of 26 kDa and consists of 209 amino acids, of which eight are Cys. The protein has three disulfide bonds between residues Cys5-Cys15, Cys65-Cys181, and Cy...

journal_title：Biochemistry

authors： Rand-Weaver M,Noso T,Muramoto K,Kawauchi H

更新日期：1991-02-12 00:00:00

abstract：:Escherichia coli dihydrofolate reductase contains five tryptophan residues that are spatially distributed throughout the protein and located in different secondary structural elements. When these tryptophans are replaced with [6-19F]tryptophan, distinct native and unfolded resonances can be resolved in the 1-D 19F NMR...

journal_title：Biochemistry

authors： Hoeltzli SD,Frieden C

更新日期：1998-01-06 00:00:00

abstract：:Human P-glycoprotein (Pgp), a plasma membrane protein that confers multidrug resistance, functions as an ATP-dependent drug efflux pump. Pgp contains two ATP binding/utilization sites and exhibits ATPase activity that is stimulated in the presence of substrates and modulating agents. The mechanism of coupling of ATP h...

journal_title：Biochemistry

authors： Ramachandra M,Ambudkar SV,Chen D,Hrycyna CA,Dey S,Gottesman MM,Pastan I

更新日期：1998-04-07 00:00:00

abstract：:The N-type voltage-gated Ca(2+) channel CaV2.2 is one of the important targets for pain management. ω-Conotoxins isolated from venoms of cone snails, which specifically inhibit CaV2.2, are promising scaffolds for novel analgesics. The inhibitory action of ω-conotoxins on CaV2.2 has been examined experimentally, but th...

journal_title：Biochemistry

authors： Chen R,Chung SH

更新日期：2013-05-28 00:00:00

abstract：:SecA-lipid interactions are believed to be important for the translocation of precursor proteins across the inner membrane of Escherichia coli [Lill, R., Dowhan, W., & Wickner, W. (1990) Cell 60, 271-280]. SecA insertion into the phospholipid bilayer could a role in this process. We investigated this possibility by st...

journal_title：Biochemistry

authors： Breukink E,Demel RA,de Korte-Kool G,de Kruijff B

更新日期：1992-02-04 00:00:00

abstract：:According to the X-ray crystallographic results from human deoxyhemoglobin, beta 99Asp at the alpha 1 Beta 2 interface forms hydrogen bonds with alpha 42Tyr and alpha 97Asn. To clarify the structural and functional roles of the hydrogen bond between alpha 97Asn and beta 99Asp, we have engineered a recombinant hemoglob...

journal_title：Biochemistry

authors： Kim HW,Shen TJ,Ho NT,Zou M,Tam MF,Ho C

更新日期：1996-05-28 00:00:00

abstract：:Phosphorylase kinase, a regulatory enzyme of glycogenolysis in skeletal muscle, is a hexadecameric oligomer consisting of four copies each of a catalytic subunit (gamma) and three regulatory subunits (alpha, beta, and delta, the last being endogenous calmodulin). The enzyme is activated by a variety of effectors actin...

journal_title：Biochemistry

authors： Nadeau OW,Traxler KW,Fee LR,Baldwin BA,Carlson GM

更新日期：1999-02-23 00:00:00

abstract：:Chorismate-utilizing enzymes catalyze diverse reactions, providing critical physiological functions unique to plants, bacteria, fungi, and some parasites. Their absence in animals makes them excellent targets for antimicrobials and herbicides. 4-Amino-4-deoxychorismate synthase (ADCS) catalyzes the first step in folat...

journal_title：Biochemistry

authors： He Z,Toney MD

更新日期：2006-04-18 00:00:00

abstract：:Large conductance Ca(2+)-activated K(+) channels (BK(Ca)) contain an intracellular binding site for bovine pancreatic trypsin inhibitor (BPTI), a well-known inhibitor of various serine proteinase (SerP) enzymes. To investigate the structural basis of this interaction, we examined the activity of 11 BPTI mutants using ...

journal_title：Biochemistry

authors： Favre I,Moss GW,Goldenberg DP,Otlewski J,Moczydlowski E

更新日期：2000-02-29 00:00:00

abstract：:The isothermal equilibrium folding of the unmodified yeast tRNA(Phe) is studied as a function of Na(+), Mg(2+), and urea concentration with hydroxyl radical protection, circular dichroism, and diethyl pyrocarbonate (DEPC) modification. These assays indicate that this tRNA folds in Na(+) alone. Similar to folding in Mg...

journal_title：Biochemistry

authors： Shelton VM,Sosnick TR,Pan T

更新日期：2001-03-27 00:00:00

abstract：:Stimulation of isolated hepatocytes with epidermal growth factor (EGF) causes rapid tyrosine phosphorylation of the EGF receptor (EGFR) and adapter/target proteins, which was monitored with 1 and 2 s resolution at 37, 20, and 4 degrees C. The temporal responses detected for multiple signaling proteins involve both tra...

journal_title：Biochemistry

authors： Moehren G,Markevich N,Demin O,Kiyatkin A,Goryanin I,Hoek JB,Kholodenko BN

更新日期：2002-01-08 00:00:00

abstract：:The constant (CL) fragment of the immunoglobulin light chain contains only one intrachain disulfide bond buried in the interior of the molecule. The kinetics of reduction with dithiothreitol of the disulfide bond were studied at various concentrations of guanidine hydrochloride at pH 8.0 and 25 degrees C. It was found...

journal_title：Biochemistry

authors： Kikuchi H,Goto Y,Hamaguchi K

更新日期：1986-04-22 00:00:00

abstract：:Various agents are able to stimulate the EGF receptor protein tyrosine kinase in the absence of ligand binding. To characterize their mechanism of action, we investigated their effects on the kinase activity of the intracellular domain of the EGF receptor (EGFR-IC). EGFR-IC (67 kDa) lacking the extracellular domain an...

journal_title：Biochemistry

authors： Mohammadi M,Honegger A,Sorokin A,Ullrich A,Schlessinger J,Hurwitz DR

更新日期：1993-08-31 00:00:00

abstract：:Azurin from Pseudomonas aeruginosa and two mutants where the methionine ligand has been mutated have been studied in order to directly investigate the functional and structural significance of this ligand in the blue copper proteins. Reduction potentials, X-ray absorption fine structure (XAFS), electron paramagnetic r...

journal_title：Biochemistry

authors： Murphy LM,Strange RW,Karlsson BG,Lundberg LG,Pascher T,Reinhammar B,Hasnain SS

更新日期：1993-03-02 00:00:00

abstract：:A high molecular weight acyl coenzyme A (acyl-CoA) thioesterase, designated thioesterase II, has been purified 5300-fold from photoheterotrophically grown cells of Rhodopseudomonas sphaeroides. In contrast to R. sphaeroides acyl-CoA thioesterase I [Boyce, S.G., & Lueking, D.R. (1984) Biochemistry 23, 141-147], thioest...

journal_title：Biochemistry

authors： Seay T,Lueking DR

更新日期：1986-05-06 00:00:00

abstract：:Substitution of Co(II) for the catalytic site Zn(II) of horse liver alcohol dehydrogenase (LADH) yields an active enzyme derivative, CoIIE, with characteristic Co(II) charge-transfer and d-d electronic transitions that are sensitive to the events which take place during catalysis [Koerber, S. C., MacGibbon, A. K. H., ...

journal_title：Biochemistry

authors： Sartorius C,Gerber M,Zeppezauer M,Dunn MF

更新日期：1987-02-10 00:00:00

abstract：:Oxidized cytochrome c oxidase exists in two reasonably well-defined conformations, a high-spin conformation with maximal absorption at 418 nm and a low-spin conformation with maximal absorption at 428 nm. The equilibrium between these two conformations has been studied as a function of pH, pressure, and temperature. p...

journal_title：Biochemistry

authors： Kornblatt JA,Hui Bon Hoa G

更新日期：1982-10-26 00:00:00

abstract：:VSTx1 is a voltage sensor toxin from the spider Grammostola spatulata that inhibits KvAP, an archeabacterial voltage-activated K(+) channel whose X-ray structure has been reported. Although the receptor for VSTx1 and the mechanism of inhibition are unknown, the sequence of the toxin is related to hanatoxin (HaTx) and ...

journal_title：Biochemistry

authors： Jung HJ,Lee JY,Kim SH,Eu YJ,Shin SY,Milescu M,Swartz KJ,Kim JI

更新日期：2005-04-26 00:00:00

abstract：:Glycophorin A is a sialoglycoprotein isolated from human erythrocyte membranes which seems to exist as stable dimeric complexes in the presence of sodium dodecyl sulfate. When analyzed by dodecyl sulfate acrylamide electrophoresis this molecule forms two PAS-stainable bands (PAS-U and PAS-2) which are reversibly inter...

journal_title：Biochemistry

authors： Furthmayr H,Marchesi VT

更新日期：1976-03-09 00:00:00

abstract：:Oxindolealanine-62 lysozyme, formed by reaction with N-bromosuccinimide and purified by using affinity chromatography, was examined in its binding of homologous oligosaccharides of N-acetylglucosamine and in its catalysis of hydrolysis and of transglycosylation of the hexasaccharide of N-acetylglucosamine. Equilibrium...

journal_title：Biochemistry

authors： Shrake A,Rupley JA

更新日期：1980-08-19 00:00:00

abstract：:Although the synthesis of RNA from a DNA template is (and must be) a generally very stable process to enable transcription of kilobase transcripts, it has long been known that during initial transcription of the first 8-10 bases of RNA complexes are relatively unstable, leading to the release of short abortive RNA tra...

journal_title：Biochemistry

authors： Vahia AV,Martin CT

更新日期：2011-08-16 00:00:00

abstract：:Fourier transform infrared (FTIR) spectroscopy was employed to examine the thermal denaturation of the Fe(III), Fe(II), and Fe(II)-CO forms of cytochrome c peroxidase and horseradish peroxidase in phosphate buffer at pD 7.0. The amide I' regions of the deconvolved spectra are consistent with predominantly alpha-helica...

journal_title：Biochemistry

authors： Holzbaur IE,English AM,Ismail AA

更新日期：1996-04-30 00:00:00

abstract：:The heat production for the reaction steps of ATP hydrolysis by heavy meromyosin has been reexamined by using a reaction-type calorimeter. So far, the values reported for the heat production have varied substantially among research groups. The present results obtained in 0.3 M KCl, 10 mM MgCl2, and 20 mM Tris-HCl at p...

journal_title：Biochemistry

authors： Yamada T,Shimizu H,Suga H

更新日期：1981-07-21 00:00:00