Reduction of the buried intrachain disulfide bond of the constant fragment of the immunoglobulin light chain: global unfolding under physiological conditions.

abstract：:The first committed step of fatty acid and polyketides biosynthesis, the biotin-dependent carboxylation of an acyl-CoA, is catalyzed by acyl-CoA carboxylases (ACCases) such as acetyl-CoA carboxylase (ACC) and propionyl-CoA carboxylase (PCC). ACC and PCC in Streptomyces coelicolor are homologue multisubunit complexes t...

journal_title：Biochemistry

authors： Arabolaza A,Shillito ME,Lin TW,Diacovich L,Melgar M,Pham H,Amick D,Gramajo H,Tsai SC

更新日期：2010-08-31 00:00:00

abstract：:Chemical-modification studies on submitochondrial particle pyridine dinucleotide transhydrogenase (EC 1.6.1.1) demonstrate the presence of one class of sulfhydryl group in the nicotinamide adenine dinucleotide phosphate (NADP) site and another peripheral to the active site. Reaction of the peripheral sulfhydryl group ...

journal_title：Biochemistry

authors： Earle SR,O'Neal SG,Fisher RR

更新日期：1978-10-31 00:00:00

abstract：:Human frataxin has a vital role in the biosynthesis of iron-sulfur (Fe-S) clusters in mitochondria, and its deficiency causes the neurodegenerative disease Friedreich's ataxia. Proposed functions for frataxin in the Fe-S pathway include iron donation to the Fe-S cluster machinery and regulation of cysteine desulfurase...

journal_title：Biochemistry

authors： Gentry LE,Thacker MA,Doughty R,Timkovich R,Busenlehner LS

更新日期：2013-09-03 00:00:00

abstract：:Two scorpion neurotoxins active only on insects, the insect toxins AaH IT1 and AaH IT2, were purified from the venom of scorpions Androctonus australis Hector collected in Tozeur (Tunisia) and characterized. AaH IT2 was sequenced and found to differ in four amino acid positions from AaH IT, the single previously seque...

journal_title：Biochemistry

authors： Loret EP,Mansuelle P,Rochat H,Granier C

更新日期：1990-02-13 00:00:00

abstract：:Potent inhibitors of human cysteine proteases of the papain family have been made and assayed versus a number of relevant family members. We describe the synthesis of peptide alpha-ketoheterocyclic inhibitors that occupy binding subsites S1'-S3 of the cysteine protease substrate recognition cleft and that form a rever...

journal_title：Biochemistry

authors： McGrath ME,Sprengeler PA,Hill CM,Martichonok V,Cheung H,Somoza JR,Palmer JT,Janc JW

更新日期：2003-12-30 00:00:00

abstract：:The increase of the proton motive force mediated by bacteriorhodopsin is compared to the intracellular ATP concentration under steady-state illumination. The membrane potential was measured via the accumulation of the lipophilic ion [14C]triphenylmethylphosphonium and the pH gradient via the accumulation of the weak a...

journal_title：Biochemistry

authors： Michel H,Oesterhelt D

更新日期：1980-09-30 00:00:00

abstract：:Pairing rules have been developed to predict the sequence specificity of minor groove binding polyamides containing pyrrole (Py) and imidazole (Im) amino acids. An Im/Py pair distinguishes G.C from C.G and both of these from A.T/T.A base pairs. A Py/Py pair appears not to distinguish A.T from T.A base pairs. To test t...

journal_title：Biochemistry

authors： White S,Baird EE,Dervan PB

更新日期：1996-09-24 00:00:00

abstract：:The structure of the ca. 250 amino acid hormone binding domain of the human estrogen receptor (hER-LBD), expressed in E. coli and purified as a complex with estradiol, has been probed by selective proteolysis, with analysis of the protein fragments both by classical methods (SDS-PAGE and Edman N-terminal sequencing) a...

journal_title：Biochemistry

authors： Seielstad DA,Carlson KE,Kushner PJ,Greene GL,Katzenellenbogen JA

更新日期：1995-10-03 00:00:00

abstract：:alpha-Glucosidase (EC 3.2.1.20) was purified to homogeneity from logarithmically growing cells of Saccharomyces carlsbergensis. The purification involved the following steps: (a) ammonium sulfate fractionation; (b) Sephadex G-100 chromatography; (c) DEAE-cellulose chromatography; and (d) hydroxylapatite chromatography...

journal_title：Biochemistry

authors： Needleman RB,Federoff HJ,Eccleshall TR,Buchferer B,Marmur J

更新日期：1978-10-31 00:00:00

abstract：:The activity of peptidyl-tRNALys-CpCp2'dA was measured in an in vitro poly(A)-dependent polypeptide synthesizing system derived from Escherichia coli. It has already been shown that Lys-tRNALys-CpCp2'dA is active as an acceptor and Ac2-Lys-tRNALys-Cp2'dA can donate its peptidyl residue but that the overall poly(A)-dep...

journal_title：Biochemistry

authors： Wagner T,Sprinzl M

更新日期：1983-01-04 00:00:00

abstract：:The light-induced QA-/QA FTIR difference spectrum of the photoreduction of the primary quinone (QA) in reaction centers (RCs) from Rhodobacter sphaeroides exhibits a set of complex differential bands between 1750 and 1715 cm(-1). Several of these features correspond in frequency to bands that bleach in the HA-/HA FTIR...

journal_title：Biochemistry

authors： Breton J,Nabedryk E,Allen JP,Williams JC

更新日期：1997-04-15 00:00:00

abstract：:Two heat-shock proteins that show high identity with the Escherichia coli chaperonin 60 (groEL) and chaperonin 10 (groES) chaperonin proteins were purified and characterized from photolithoautotrophically grown Rhodobacter sphaeroides. The proteins were purified by using sucrose density gradient centrifugation and Mon...

journal_title：Biochemistry

authors： Terlesky KC,Tabita FR

更新日期：1991-08-20 00:00:00

abstract：:The binding of Sp1 transcription factor to DNA is considered a potential target for small ligands designed to interfere with gene transcription. We attempted to distinguish the direct inhibition of the Sp1-binding to DNA in vivo (cell culture) from more indirect effects due to the network of pathways that modulate cel...

journal_title：Biochemistry

authors： Mansilla S,Priebe W,Portugal J

更新日期：2004-06-15 00:00:00

abstract：:The structural and motional properties of membrane lipids in various physical states and macroscopic organizations are elucidated by 1H NMR dipolar echo decay spectroscopy (DECODE). Multilamellar lipid dispersions in the gel (L beta') and liquid-crystalline (L alpha) states and a nonbilayer, hexagonal (HII) phase are ...

journal_title：Biochemistry

authors： Janes N,Rubin E,Taraschi TF

更新日期：1990-09-11 00:00:00

abstract：:The catalytic utilization of dimethylallyl, geranyl, farnesyl, and geranylgeranyl diphosphates in the reaction catalyzed by recombinant human farnesyl:protein transferase (hFPTase) has been examined in the presence of three different protein substrates, Ras-CVLS, Ras-CVIM, and Ras-CAIL. hFPTase catalyzed both farnesyl...

journal_title：Biochemistry

authors： Pompliano DL,Schaber MD,Mosser SD,Omer CA,Shafer JA,Gibbs JB

更新日期：1993-08-17 00:00:00

abstract：:The microscopic interactions and mechanisms leading to nascent protein folding events are generally unknown. While such short time-scale events are difficult to study experimentally, molecular dynamics simulations of peptides can provide a useful model for studying events related to protein folding initiation. Recentl...

journal_title：Biochemistry

authors： Karpen ME,Tobias DJ,Brooks CL 3rd

更新日期：1993-01-19 00:00:00

abstract：:The fungal neurotoxin alpha-cyclopiazonic acid (CPA), a nanomolar inhibitor of Ca(2+)-ATPase with a unique pentacyclic indole tetramic acid scaffold, is assembled by a three enzyme pathway CpaS, CpaD, and CpaO in Aspergillus sp. We recently characterized the first pathway-specific enzyme CpaS, a hybrid two module poly...

journal_title：Biochemistry

authors： Liu X,Walsh CT

更新日期：2009-11-24 00:00:00

abstract：:Electrostatic interactions play important roles in the catalysis of chorismate to prephenate by chorismate mutase. Mutation of Gln88 to glutamate in the monofunctional chorismate mutase from Escherichia coli results in an enzyme with a pH profile of activity significantly different from that of the wild type protein. ...

journal_title：Biochemistry

authors： Lee AY,Zhang S,Kongsaeree P,Clardy J,Ganem B,Erickson JW,Xie D

更新日期：1998-06-23 00:00:00

abstract：:Binding of substrate to ornithine 4,5-aminomutase (OAM) and methylmalonyl-CoA mutase (MCM) leads to the formation of an electrostatic interaction between a conserved glutamate side chain and the adenosyl ribose of the adenosylcobalamin (AdoCbl) cofactor. The contribution of this residue (Glu338 in OAM from Clostridium...

journal_title：Biochemistry

authors： Makins C,Pickering AV,Mariani C,Wolthers KR

更新日期：2013-02-05 00:00:00

abstract：:Studies of intragenomic homologues in bacterial genomes can provide valuable insights into functional divergence. Three GTP cyclohydrolase II homologues in the Streptomyces coelicolor genome have been shown to catalyze two related but distinct reactions [Spoonamore, J. E., Dahlgran, A. L., Jacobsen, N. E., and Bandari...

journal_title：Biochemistry

authors： Spoonamore JE,Bandarian V

更新日期：2008-02-26 00:00:00

abstract：:Amyloid beta (Abeta) peptide amyloidogenesis, involving the formation of numerous distinct quaternary structures, appears to cause Alzheimer's disease. However, the precise identification of the toxic structure(s) and the neurotoxicity mechanism(s) remains elusive. Mutating the Abeta 1-40 Phe19-Phe20 backbone amide bo...

journal_title：Biochemistry

authors： Bieschke J,Siegel SJ,Fu Y,Kelly JW

更新日期：2008-01-08 00:00:00

abstract：:The bacterial photosynthetic reaction center is the evolutionary ancestor of the Photosystem II reaction center. These proteins share the same fold and perform the same biological function. Nevertheless, the details of their molecular reaction mechanism differ. It is of significant biological and biochemical interest ...

journal_title：Biochemistry

authors： Krammer EM,Sebban P,Ullmann GM

更新日期：2009-02-17 00:00:00

abstract：:Dihydroorotates deuteriated at both C5 and C6 have been prepared and used to probe the mechanism of the bovine liver mitochondrial dihydroorotate dehydrogenase. Primary deuterium isotope effects on kcat are observed with both (6RS)-[5(S)-2H]- and (6RS)-[6-2H] dihydroorotates (3 and 6, respectively); these effects are ...

journal_title：Biochemistry

authors： Hines V,Johnston M

更新日期：1989-02-07 00:00:00

abstract：:The tau microtubule-associated proteins are axonal proteins that have been implicated in axonal outgrowth, microtubule spacing, and microtubule bundling. Moreover, tau is the major structural component of the paired helical filaments present in the brains of Alzheimer's disease patients. The Caenorhabditis elegans Gen...

journal_title：Biochemistry

authors： McDermott JB,Aamodt S,Aamodt E

更新日期：1996-07-23 00:00:00

abstract：:The X-ray structures of the ligand free (apo) and the Mg(2+)*o-succinylbenzoate (OSB) product complex of o-succinylbenzoate synthase (OSBS) from Escherichia coli have been solved to 1.65 and 1.77 A resolution, respectively. The structure of apo OSBS was solved by multiple isomorphous replacement in space group P2(1)2(...

journal_title：Biochemistry

authors： Thompson TB,Garrett JB,Taylor EA,Meganathan R,Gerlt JA,Rayment I

更新日期：2000-09-05 00:00:00

abstract：:Esterification of retinol (vitamin A alcohol) with long-chain fatty acids by lecithin-retinol acyltransferase (LRAT) is an important step in both the absorption and storage of vitamin A. Retinol in cells is bound by either cellular retinol binding protein (CRBP), present in most tissues including liver, or cellular re...

journal_title：Biochemistry

authors： Herr FM,Ong DE

更新日期：1992-07-28 00:00:00

abstract：:We reacted uracil-containing M13mp2 DNA with N-hydroxy-2-aminofluorene to produce a template with N-(deoxyguanosin-8-yl)-2-aminofluorene adducts. This template was hybridized to a non-uracil-containing linear fragment from which the lac z complementing insert had been removed to produce a gapped substrate. DNA synthes...

journal_title：Biochemistry

authors： Sahm J,Turkington E,LaPointe D,Strauss B

更新日期：1989-04-04 00:00:00

abstract：:It is well-known that caldesmon (CaD) is a substrate for casein kinase II (CKII), and the phosphorylation of CaD by CKII regulates the interaction of CaD with myosin. However, the functionally relevant CKII phosphorylation site(s) on CaD and the precise role of CaD phosphorylation by CKII in mediating CaD's function h...

journal_title：Biochemistry

authors： Wang Z,Yang ZQ

更新日期：2000-09-12 00:00:00

abstract：:Multidrug resistance protein (MRP) and P-glycoprotein (Pgp) are both members of the superfamily of ATP binding cassette plasma membrane drug transport proteins, which may be partly responsible for multidrug resistance of tumor cells. Although MRP has been identified as an organic anion transporter and Pgp as a transpo...

journal_title：Biochemistry

authors： Essodaigui M,Broxterman HJ,Garnier-Suillerot A

更新日期：1998-02-24 00:00:00

abstract：:The HAMP domain is a conserved motif widely distributed in prokaryotic and lower eukaryotic organisms, where it is often found in transmembrane receptors that regulate two-component signaling pathways. The motif links receptor input and output modules and is essential to receptor structure and signal transduction. Rec...

journal_title：Biochemistry

authors： Swain KE,Falke JJ

更新日期：2007-12-04 00:00:00