Abstract：
:Two heat-shock proteins that show high identity with the Escherichia coli chaperonin 60 (groEL) and chaperonin 10 (groES) chaperonin proteins were purified and characterized from photolithoautotrophically grown Rhodobacter sphaeroides. The proteins were purified by using sucrose density gradient centrifugation and Mono-Q anion-exchange chromatography. In the presence of 1 mM ATP, the chaperonin 10 and chaperonin 60 proteins bound to each other and comigrated as a large complex during sucrose density gradient centrifugation. The native molecular weights of each protein as determined by gel filtration chromatography were 889,200 for chaperonin 60 and 60,000 for chaperonin 10. Chaperonin 60 is comprised of monomers with a molecular weight of 61,000 and chaperonin 10 is comprised of monomers with a molecular weight of 12,700 as determined by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. Chaperonin 60 was 9.3% of the total soluble cell protein during photolithoautotrophic growth which increased to 28.5% following heat-shock treatment. When cells were grown photoheterotrophically or chemoheterotrophically, chaperonin 60 was reduced to 6.7% and 3.5%, respectively, of the total soluble protein. The N-terminal amino acid sequence of each protein was determined; chaperonin 60 of R. sphaeroides showed 72% identity to E. coli chaperonin 60 protein, and R. sphaeroides chaperonin 10 showed 45% identity with E. coli chaperonin 10. R. sphaeroides chaperonin 60 catalyzed ATP hydrolysis with a specific activity of 134 nmol min-1 mg-1 (kcat = 0.13 s-1) and was inhibited by R. sphaeroides chaperonin 10, but not E. coli chaperonin 10. The E. coli chaperonin 60 ATPase activity was inhibited by chaperonin 10 from both R. sphaeroides and E. coli.
journal_name
Biochemistryjournal_title
Biochemistryauthors
Terlesky KC,Tabita FRdoi
10.1021/bi00247a013subject
Has Abstractpub_date
1991-08-20 00:00:00pages
8181-6issue
33eissn
0006-2960issn
1520-4995journal_volume
30pub_type
杂志文章相关文献
BIOCHEMISTRY文献大全abstract：:The human pathogen Staphylococcus aureus acquires heme iron from hemoglobin (Hb) via the action of a series of iron-regulated surface determinant (Isd) proteins. The cell wall anchored IsdB protein is recognized as the predominant Hb receptor, and is comprised of two NEAr transporter (NEAT) domains that act in concert...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi5005188
更新日期：2014-06-24 00:00:00
abstract：:Antifreeze proteins lower the freezing point of their solution by binding to ice and inhibiting its growth. One of several structurally different antifreeze proteins in fishes (type II) is homologous to the carbohydrate-recognition domain of Ca2+-dependent lectins and adopts the same three-dimensional fold. Type II an...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi9820513
更新日期：1998-12-22 00:00:00
abstract：:Raman spectroscopy was used to study the phase behavior of several semisynthetic species of the acidic glycosphingolipid cerebroside sulfate (CBS) which occur in myelin. The C-H stretching mode region at 2800-3100 cm-1 of C18:0-CBS, C24:0-CBS, and C26:0-CBS, and the alpha-hydroxy fatty acid species C18:0h-CBS, was stu...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00121a041
更新日期：1992-02-18 00:00:00
abstract：:Ornithine decarboxylase (ODC) is a pyridoxal 5'-phosphate (PLP) dependent homodimeric enzyme. It is a recognized drug target against African sleeping sickness, caused by Trypanosoma brucei. One of the currently used drugs, alpha-difluoromethylornithine (DFMO), is a suicide inhibitor of ODC. The structure of the T. bru...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi9915115
更新日期：1999-11-16 00:00:00
abstract：:Drosophila Rrp1 includes a carboxy-terminal region homologous to Escherichia coli exonuclease III which is sufficient to repair both oxidative and alkylation damage to DNA. An apurinic/apyrimidinic endonuclease activity intrinsic to Rrp1 was characterized previously. In this work, the 3'-phosphodiesterase and 3'-phosp...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00004a021
更新日期：1995-01-31 00:00:00
abstract：:The mechanisms of MRP1-drug binding and transport are not clear. In this study, we have characterized the interaction between MRP1 and rhodamine 123 (Rh123) using the photoreactive-iodinated analogue, [(125)I]iodoaryl azido-rhodamine 123 (or IAARh123). Photoaffinity labeling of plasma membranes from HeLa cells transfe...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi0020574
更新日期：2000-12-19 00:00:00
abstract：:The (1)H NMR resonances of the heme substituents of the low-spin Fe(III) form of nitrophorin 2, as its complexes with N-methylimidazole (NP2-NMeIm) and imidazole (NP2-ImH), have been assigned by a combination of (1)H homonuclear two-dimensional NMR techniques and (1)H-(13)C HMQC. Complete assignment of the proton and ...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi026765w
更新日期：2003-01-28 00:00:00
abstract：:Photosensitized oxidation reactions damage tissue by catalyzing the formation of oxyradicals and singlet oxygen. beta-Carotene is hypothesized to exert photoprotective effects by quenching singlet oxygen formed by Type II reactions and by scavenging free radicals formed by Type I reactions. beta-Carotene antioxidant m...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi9708646
更新日期：1997-10-21 00:00:00
abstract：:Recently, it has been shown that the activation of the Drosophila EGF receptor (DER) by its natural ligand Spitz is inhibited by Argos [Schweitzer, R., et al. (1995) Nature 376, 699-702]. Argos and Spitz both have an EGF-like domain which in the case of Argos differs from that of Spitz and other EGF receptor agonists ...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi970227f
更新日期：1997-06-17 00:00:00
abstract：:alpha-Glucosidase (EC 3.2.1.20) was purified to homogeneity from logarithmically growing cells of Saccharomyces carlsbergensis. The purification involved the following steps: (a) ammonium sulfate fractionation; (b) Sephadex G-100 chromatography; (c) DEAE-cellulose chromatography; and (d) hydroxylapatite chromatography...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00615a011
更新日期：1978-10-31 00:00:00
abstract：:Kinetic models are presented that allow the Na(+),K(+)-ATPase steady-state turnover number to be estimated at given intra- and extracellular concentrations of Na(+), K(+), and ATP. Based on experimental transient kinetic data, the models utilize either three or four steps of the Albers-Post scheme, that is, E(2) --> E...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi0355443
更新日期：2004-03-02 00:00:00
abstract：:A chromatin-bound protease, active in 2 M NaCl-5 M urea or 5 M urea alone, was demonstrated in rat liver, kidney, testes, brain, rabbit bone marrow, chicken reticulocyte, and Ehrlich ascites chromatin. Chicken erythrocyte chromatin did not possess any detectable proteolytic activity in salt and urea. The proteolytic a...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00646a028
更新日期：1976-01-13 00:00:00
abstract：:Single-stranded microRNAs (miRNAs) regulate gene expression by triggering mRNA degradation and/or inhibiting mRNA translation. miRNAs play important roles in various critical cellular processes and are associated with numerous human diseases, including cancer and neurodegenerative diseases. miRNA sequences are embedde...
journal_title：Biochemistry
pub_type： 杂志文章,评审
doi：10.1021/acs.biochem.8b00944
更新日期：2019-01-29 00:00:00
abstract：:Polyphosphate glucokinase from Mycobacterium tuberculosis catalyzes the phosphorylation of glucose using inorganic polyphosphates [poly(P)] or ATP. The steady-state kinetic mechanisms of the poly(P)- and ATP-dependent glucokinase reactions were investigated using initial velocity, product inhibition, and dead-end inhi...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi9528659
更新日期：1996-07-30 00:00:00
abstract：:Polyclonal antibody to mitochondrial P-450c27/25 reacted with two proteins of apparent molecular masses of 52 kilodaltons (kDa) and 50 kDa from the female rat liver mitochondrial proteins bound to an omega-octylaminoagarose column. The two proteins were purified to greater than 85% homogeneity by DEAE-Sephacel and hyd...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00098a007
更新日期：1991-08-27 00:00:00
abstract：:The FMN prosthetic group of flavocytochrome b2 or L-lactate dehydrogenase oxidizes lactate to pyruvate. The reducing equivalents are then transferred one by one, intramolecularly, to heme b2 and then to external acceptors. Substrate oxidation is thought to begin with abstraction of the substrate alpha-hydrogen as a pr...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi9529519
更新日期：1996-07-02 00:00:00
abstract：:The formation of closed icosahedral capsids from a single species of coat protein subunit requires that the subunits assume different conformations at different lattice positions. In the double-stranded DNA bacteriophage P22, formation of correctly dimensioned capsids is mediated by interaction between coat protein su...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00475a030
更新日期：1990-06-12 00:00:00
abstract：:Proteorhodopsins are a recently discovered class of microbial rhodopsins, ubiquitous in marine bacteria. Over 4000 variants have thus far been discovered, distributed throughout the oceans of the world. Most variants fall into one of two major groups, green- or blue-absorbing proteorhodopsin (GPR and BPR, respectively...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi7018964
更新日期：2008-03-18 00:00:00
abstract：:Treatment of cells with the enediyne C-1027 is highly efficient at inducing single- and double-strand DNA breaks. This agent is highly cytotoxic when used at picomolar levels over a period of days. For this study, C-1027 has been used at higher levels for a much shorter time period to look at early cellular responses ...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi015680c
更新日期：2001-12-04 00:00:00
abstract：:The question of the possible identity of catalytic and regulatory proton pathways in the chloroplast FoF1 ATPase has been studied using different energy-transfer inhibitors. Venturicidin, a reversible inhibitor of Fo, affects neither the delta mu H(+)-dependent thiol reduction of the membrane-bound chloroplast ATPase ...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00132a013
更新日期：1992-05-05 00:00:00
abstract：:The homeodomain proteins a1 and alpha 2 act cooperatively to regulate cell type specific genes in yeast. The basis of the cooperativity is a weak interaction between the two proteins which forms heterodimers that bind DNA tightly and specifically. In this paper, we examine the mechanism of heterodimerization. We show ...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00197a033
更新日期：1994-08-09 00:00:00
abstract：:The monoamine transporter of the chromaffin granule membranes can be specifically labeled by the photoaffinity reagent 7-azido-8-[125I]iodoketanserin. The characteristics of the labeled protein have been investigated. Two-dimensional gel electrophoresis of the labeled membranes indicated a MW of about 70,000 and an is...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00122a012
更新日期：1992-02-25 00:00:00
abstract：:We have studied the tridecadeoxynucleotide CGCGAATTACGCG (I), which contains an additional A at position 9 compared to the dodecanucleotide of which the crystal structure has been determined. Sequence I exhibits no distinct melting curve and also has a concentration-dependent pattern of peaks on reverse-phase chromato...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00371a025
更新日期：1986-11-18 00:00:00
abstract：:NMR spectroscopy has been used to study the interaction of aminoglycoside antibiotics with an aminoglycoside antibiotic 3'-phosphotransferase [APH(3')-IIIa]. APH(3')-IIIa is an enterococcal enzyme that is responsible for the ATP-dependent O-phosphorylation of a broad range of aminoglycoside antibiotics. The NMR method...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi9626822
更新日期：1997-03-04 00:00:00
abstract：:A new method of calculation, based on a direct fitting of the protein fluorescence intensity observed upon coenzyme binding (H.-P. Lutz, unpublished results), is used to study the negative cooperative behavior of glyceraldehyde-3-phosphate dehydrogenase from sturgeon muscle. The calculation procedure simultaneously el...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00288a014
更新日期：1983-09-13 00:00:00
abstract：:Substrates homoprotocatechuate (HPCA) and O(2) bind to the Fe(II) of homoprotocatechuate 2,3-dioxygenase (FeHPCD) in adjacent coordination sites. Transfer of an electron(s) from HPCA to O(2) via the iron is proposed to activate the substrates for reaction with each other to initiate aromatic ring cleavage. Here, rapid...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi201436n
更新日期：2011-11-29 00:00:00
abstract：:Homoserine kinase, an enzyme in the aspartate pathway of amino acid biosynthesis in Escherichia coli, catalyzes the conversion of L-homoserine to L-homoserine phosphate. This enzyme has been found to have broad substrate specificity, including the phosphorylation of L-homoserine analogs where the carboxyl functional g...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi962203z
更新日期：1996-12-17 00:00:00
abstract：:Human NFU (also known as HIRIP5) has been implicated in cellular iron-sulfur cluster biosynthesis. Bacterial and yeast forms are smaller than the human protein and are homologous to the C-terminal domain of the latter. This C-terminal domain contains a pair of redox active cysteines and demonstrates thioredoxin-like a...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi801645z
更新日期：2009-02-10 00:00:00
abstract：:We have determined the structures of three novel oligosaccharides isolated from human milk using the monoclonal antibody MSW 113. These oligosaccharides were purified by affinity chromatography on a column of the immobilized monoclonal antibody and by high-performance liquid chromatography. From the results of 500-MHz...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00448a031
更新日期：1989-10-31 00:00:00
abstract：:The mechanism of the slow refolding reactions of four different pancreatic ribonucleases from ox, sheep, red deer, and roe deer has been investigated. Refolding kinetics of these proteins were very similar. In particular, a native-like intermediate, IN, was shown to be populated on the slow refolding pathway of all ri...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00336a005
更新日期：1985-07-16 00:00:00
