Abstract：
:Single-stranded microRNAs (miRNAs) regulate gene expression by triggering mRNA degradation and/or inhibiting mRNA translation. miRNAs play important roles in various critical cellular processes and are associated with numerous human diseases, including cancer and neurodegenerative diseases. miRNA sequences are embedded in the primary miRNA transcripts (pri-miRNAs) that are initially processed by the Microprocessor complex in the nucleus. Microprocessor can orient itself on pri-miRNAs in two ways: one orientation results in subsequent miRNA production, and the other leads to cleavage of the miRNA sequence. Therefore, orienting Microprocessor on pri-miRNAs is a fundamental mechanism for determining the accuracy and efficiency of pri-miRNA processing and, in turn, miRNA production. Multiple mechanisms controlling Microprocessor orientation on pri-miRNAs, involving both cis-acting RNA elements and trans-acting factors, have recently been revealed. In this review, we discuss these exciting mechanisms and consider potential unknown mechanisms that might regulate Microprocessor orientation on pri-miRNAs.
journal_name
Biochemistryjournal_title
Biochemistryauthors
Nguyen HM,Nguyen TD,Nguyen TL,Nguyen TAdoi
10.1021/acs.biochem.8b00944subject
Has Abstractpub_date
2019-01-29 00:00:00pages
189-198issue
4eissn
0006-2960issn
1520-4995journal_volume
58pub_type
杂志文章,评审相关文献
BIOCHEMISTRY文献大全abstract：:A description of the efficient high-level expression of the monomer hemoglobin (GMG4) from Glycera dibranchiata is presented. The cDNA described by Simons and Satterlee [Simons, P.C., & Satterlee, J.D. (1989) Biochemistry 28, 8525-8530] was subcloned into an expression system, and conditions were found that led to the...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00200a014
更新日期：1994-08-30 00:00:00
abstract：:The bidirectional transformation of a protein between its native water-soluble and integral transmembrane conformations is demonstrated for FraC, a hemolytic protein of the family of pore-forming toxins. In the presence of biological membranes, the water-soluble conformation of FraC undergoes a remarkable structural r...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/acs.biochem.5b01112
更新日期：2015-11-24 00:00:00
abstract：:Parathyroid hormone (PTH) regulates calcium and phosphate metabolism through a G-protein-coupled receptor which is shared with PTH-related protein (PTHrP). Therefore, structure-activity studies of PTH and PTHrP with their common receptor provide an unusual opportunity to examine the structural elements in the two horm...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00033a029
更新日期：1995-08-22 00:00:00
abstract：:Casein kinase II is a widely distributed protein serine/threonine kinase. The holoenzyme appears to be a tetramer, containing two alpha or alpha' subunits (or one of each) and two beta subunits. Complementary DNA clones encoding the subunits of casein kinase II were isolated from a human T-cell lambda gt10 library usi...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00488a034
更新日期：1990-09-11 00:00:00
abstract：:Lon is a homo-oligomeric ATP-dependent serine protease that functions in the degradation of damaged and certain regulatory proteins. This enzyme has emerged as a novel target in the development of antibiotics because of its importance in conferring bacterial virulence. In this study, we explored the mechanism by which...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi7002789
更新日期：2007-06-05 00:00:00
abstract：:Several strains of Sphingobium chlorophenolicum have been isolated from soil that was heavily contaminated with pentachlorophenol (PCP), a toxic pesticide introduced in the 1930s. S. chlorophenolicum appears to have assembled a poorly functioning pathway for degradation of PCP by patching enzymes recruited via two ind...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi300261p
更新日期：2012-05-08 00:00:00
abstract：:Bacimethrin-derived 2'-methoxythiamin pyrophosphate inhibits microbial growth by disrupting metabolic pathways dependent on thiamin-utilizing enzymes. This study describes the discovery of the bacimethrin biosynthetic gene cluster of Clostridium botulinum A ATCC 19397 and in vitro reconstitution of bacimethrin biosynt...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi500281a
更新日期：2014-04-15 00:00:00
abstract：:Rabbit muscle lactate dehydrogenase (LDH) was coupled to Sepharose in such a way that each molecule is expected to be attached via only one subunit. Dissociation of the bound active enzyme by several methods all yielded immobilized subunit derivatives which were inactive. These derivatives were capable of regenerating...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00664a013
更新日期：1976-09-21 00:00:00
abstract：:To probe the structure and stability of the central region of lipid-free apolipoprotein (apo) A-I (residues 123-165), we studied the effects of four mutations made in this region on the conformation, stability, dimyristoylphosphatidylcholine (DMPC) binding kinetics, and size of discoidal reconstituted high-density lip...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi025807d
更新日期：2002-08-20 00:00:00
abstract：:Dog serum and colostral immunoglobulin A (IgA) and free secretory component from colostrum were isolated using affinity chromatography. Both serum and colostral IgA showed similar susceptibility to reduction with dithiothreitol, but only colostral IgA released the additional subunit, bound secretory component. This re...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00684a010
更新日期：1975-07-01 00:00:00
abstract：:NAD(P) aldehyde dehydrogenases (EC 1.2.1.3) are a family of enzymes that oxidize a wide variety of aldehydes into acid or activated acid compounds. Using site-directed mutagenesis, the essential nucleophilic Cys 149 in the NAD-dependent phosphorylating glyceraldehyde-3-phosphate dehydrogenase from Escherichia coli has...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00164a033
更新日期：1992-12-15 00:00:00
abstract：:Near-infrared FT-Raman spectroscopy can be used to measure the vibrations of the bacteriorhodopsin (bR) chromophore without the disadvantage of conventional visible resonance Raman spectroscopy, where the visible excitation drives the bR photoreactions. We utilized this technique to investigate the light-dark adaptati...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00060a020
更新日期：1993-03-09 00:00:00
abstract：:The X-ray crystal structures of Escherichia coli gamma-aminobutyrate aminotransferase unbound and bound to the inhibitor aminooxyacetate are reported. The enzyme crystallizes from ammonium sulfate solutions in the P3(2)21 space group with a tetramer in the asymmetric unit. Diffraction data were collected to 2.4 A reso...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi049218e
更新日期：2004-08-31 00:00:00
abstract：:The RING or Really Interesting New Gene represents a family of eukaryotic sequences that bind Zn (II) ions and participate in intracellular processes involving protein-protein interaction. Although found in over 400 different proteins, very little is known regarding the structure-function properties of these domains b...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi701949p
更新日期：2008-03-25 00:00:00
abstract：:Imidazole glycerol phosphate synthase (ImGPS) from Thermotoga maritima is a model enzyme for studying allostery. The ImGPS complex consists of the cyclase subunit HisF and the glutaminase subunit HisH whose activity is stimulated by substrate binding to HisF in a V-type manner. To investigate the significance of a put...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/acs.biochem.0c00332
更新日期：2020-07-28 00:00:00
abstract：:A galactoside-binding lectin (hL-31) containing a collagen-like sequence was identified in human tumor cells. It was found to be the homologue of the IgE-binding protein, the macrophage cell-surface Mac-2 antigen, and the murine CBP35, RL-29, and mL-34 lectins. Here we report on the expression in Escherichia coli and ...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00067a038
更新日期：1993-04-27 00:00:00
abstract：:High-resolution nitrite soaked oxidized and reduced crystal structures of two active site mutants, D98N and H255N, of nitrite reductase (NIR) from Alcaligenes faecalis S-6 were determined to better than 2.0 A resolution. In the oxidized D98N nitrite-soaked structures, nitrite is coordinated to the type II copper via i...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi0107400
更新日期：2001-08-07 00:00:00
abstract：:We have determined the formal potential values for each electron transfer to electron transfer flavoprotein-ubiquinone oxidoreductase (ETF-QO), in order to further characterize the thermodynamics of electron transport from various acyl-CoA thioesters to the mitochondrial ubiquinone pool. ETF-QO contains one [4Fe-4S]2+...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00162a012
更新日期：1992-12-01 00:00:00
abstract：:The three-dimensional structure of amyloid beta peptide (25-35), which has neurotoxic activity, in lithium dodecyl sulfate micelles was determined by two-dimensional 1H NMR spectroscopy with simulated annealing calculations. A total of 20 converged amyloid beta peptide structures were obtained on the basis of 110 expe...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi961598j
更新日期：1996-12-17 00:00:00
abstract：:Using cysteine mutagenesis and chemical modification by methanethiosulfonate derivatives, it was demonstrated that the external putative loop, joining transmembrane segments (TM's) IV-V of rabbit Na+/glucose cotransporter, rSGLT1, forms part of a Na+ binding and voltage sensing domain. Within this region, exposure to ...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi052267m
更新日期：2006-04-11 00:00:00
abstract：:Sequential 1H NMR assignments of a metallothionein from Neurospora crassa have been accomplished by the combined use of COSY, 2QF-COSY, HOHAHA, and rotating-frame NOE experiments. All potentially observable resonances were assigned except for the epsilon-NH3 group of the C-terminal lysine. 1H NOEs, when observed in th...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00433a038
更新日期：1989-04-04 00:00:00
abstract：:The properties of the nucleotide binding sites in the isolated beta and alpha subunits of H(+)-ATPase from Bacillus PS3 (TF1) have been examined by studying the EPR properties of bound VO(2+), which is a paramagnetic probe for the native Mg2+ cation cofactor. The amino acid ligands of the VO2+ complexes with the isola...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi9910732
更新日期：1999-11-16 00:00:00
abstract：:Steroid-receptor complexes formed at low temperature and ionic strength are unable to bind to target cell nuclei. After a temporary exposure to high ionic strength and/or temperature they become activated (i.e., able to bind to nuclei). However, there exists an equilibrium between activated and nonactivated complexes;...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00664a025
更新日期：1976-09-21 00:00:00
abstract：:Thermal unfolding of discoidal complexes of apolipoprotein (apo) C-1 with dimyristoyl phosphatidylcholine (DMPC) reveals a novel mechanism of lipoprotein stabilization that is based on kinetics rather than thermodynamics. Far-UV CD melting curves recorded at several heating/cooling rates from 0.047 to 1.34 K/min show ...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi025588w
更新日期：2002-06-11 00:00:00
abstract：:The nicotinic acetylcholine receptor (nAChR) from Torpedo marmorata carries two nonequivalent agonist binding sites at the alphadelta and alphagamma subunit interfaces. These sites have been characterized by time-resolved fluorescence with the partial nicotinic agonist dansyl-C(6)-choline (Dnscho). When bound to the d...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi992811p
更新日期：2000-06-13 00:00:00
abstract：:NLRP4 is a member of the nucleotide-binding and leucine-rich repeat receptor (NLR) family of cytosolic receptors and a member of an inflammation signaling cascade. Here, we present the crystal structure of the NLRP4 pyrin domain (PYD) at 2.3 Å resolution. The NLRP4 PYD is a member of the death domain (DD) superfamily ...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi3007059
更新日期：2012-09-18 00:00:00
abstract：:Five gangliosides, accounting for over 95% of the total ganglioside fraction, were isolated from bovine adrenal medulla by preparative thin-layer chromatography and the carbohydrate structures determined by a combination of periodate oxidation and permethylation techniques. Partially methylated alditol acetates were g...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00678a026
更新日期：1975-04-08 00:00:00
abstract：:Isothiouronium compounds [Hoving, S., Bar-Shimon, M., Tijmes, J. J., Goldshleger, R., Tal, D. M., and Karlish, S. J. (1995) J. Biol. Chem. 270, 29788-29793] act as high-affinity competitive antagonists for Na(+) and K(+) (Rb(+)) on the renal Na(+)/K(+)-ATPase where they favor the E1 conformation. We have now character...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi026071n
更新日期：2003-04-01 00:00:00
abstract：:We have investigated the loss of histone H1 from chromatin [D'Anna, J. A., Gurley, L. R., & Tobey, R. A. (1982) Biochemistry 21, 3991-4001] and the structure of chromatin from Chinese hamster (line CHO) cells blocked in early S phase by sequential use of isoleucine deprivation G1 block and 1 mM hydroxyurea (HU) blocka...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00293a027
更新日期：1983-11-22 00:00:00
abstract：:The role of the interaction between actin and the secondary actin binding site of myosin (segment 565-579 of rabbit skeletal muscle myosin, referred to as loop 3 in this work) has been studied with proteolytically generated smooth and skeletal muscle myosin subfragment 1 and recombinant Dictyostelium discoideum myosin...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi991595h
更新日期：1999-11-16 00:00:00
