Orientation of Human Microprocessor on Primary MicroRNAs.


:Single-stranded microRNAs (miRNAs) regulate gene expression by triggering mRNA degradation and/or inhibiting mRNA translation. miRNAs play important roles in various critical cellular processes and are associated with numerous human diseases, including cancer and neurodegenerative diseases. miRNA sequences are embedded in the primary miRNA transcripts (pri-miRNAs) that are initially processed by the Microprocessor complex in the nucleus. Microprocessor can orient itself on pri-miRNAs in two ways: one orientation results in subsequent miRNA production, and the other leads to cleavage of the miRNA sequence. Therefore, orienting Microprocessor on pri-miRNAs is a fundamental mechanism for determining the accuracy and efficiency of pri-miRNA processing and, in turn, miRNA production. Multiple mechanisms controlling Microprocessor orientation on pri-miRNAs, involving both cis-acting RNA elements and trans-acting factors, have recently been revealed. In this review, we discuss these exciting mechanisms and consider potential unknown mechanisms that might regulate Microprocessor orientation on pri-miRNAs.






Nguyen HM,Nguyen TD,Nguyen TL,Nguyen TA




Has Abstract


2019-01-29 00:00:00












  • Identification and metabolic transformations of carotenoids in ocular tissues of the Japanese quail Coturnix japonica.

    abstract::As in humans and monkeys, lutein [(3R,3'R,6'R)-beta,epsilon-carotene-3,3'-diol] and zeaxanthin [a mixture of (3R,3'R)-beta,beta-carotene-3,3'diol and (3R,3'S-meso)-beta,beta-carotene-3,3'-diol] are found in substantial amounts in the retina of the Japanese quail Coturnix japonica. This makes the quail retina an excell...


    pub_type: 杂志文章


    authors: Bhosale P,Serban B,Zhao DY,Bernstein PS

    更新日期:2007-08-07 00:00:00

  • Ionization equilibria for side-chain carboxyl groups in oxidized and reduced human thioredoxin and in the complex with its target peptide from the transcription factor NF kappa B.

    abstract::The pH dependence of the 13C chemical shifts of the side-chain carboxyl carbons of all Asp and Glu residues in the reduced and oxidized states of human thioredoxin and in a mixed disulfide complex of human thioredoxin with a target peptide from the transcription factor NF kappa B has been investigated by multidimensio...


    pub_type: 杂志文章


    authors: Qin J,Clore GM,Gronenborn AM

    更新日期:1996-01-09 00:00:00

  • Inhibition of eukaryotic translation by analogues of messenger RNA 5'-cap: chemical and biological consequences of 5'-phosphate modifications of 7-methylguanosine 5'-monophosphate.

    abstract::New analogues of 7-methylguanosine 5'-monophosphate (m7GMP) were synthesized with modified 5'-phosphate moieties by replacement of -O with -H, -CH3, or -NH2. Additional analogues were synthesized with 8-methyl- or 8-aminoguanine base substitutions or ring-opened ribose (2',3'-diol). These compounds were analyzed by 1H...


    pub_type: 杂志文章


    authors: Darzynkiewicz E,Ekiel I,Lassota P,Tahara SM

    更新日期:1987-07-14 00:00:00

  • Temperature-induced conformational switch in intestinal fatty acid binding protein (IFABP) revealing an alternative mode for ligand binding.

    abstract::IFABP is a small beta-barrel protein with a short helix-turn-helix motif near the N-terminus that is thought to participate in the regulation of the uptake and delivery of fatty acids. In a previous work, we detected by near UV circular dichroism a reversible conformational transition of this protein occurring between...


    pub_type: 杂志文章


    authors: Arighi CN,Rossi JP,Delfino JM

    更新日期:2003-06-24 00:00:00

  • Involvement of Phenylalanine 297 in the Construction of the Substrate Pocket of Human Aminopeptidase B.

    abstract::Aminopeptidase B (APB, EC preferentially hydrolyzes the N-terminal basic amino acids of synthetic and peptide substrates and requires a physiological concentration of NaCl for optimal activity. In this study, we used site-directed mutagenesis and molecular modeling to search for an amino acid residue that is...


    pub_type: 杂志文章


    authors: Ohnishi A,Watanabe J,Ogawa Y,Goto Y,Hattori A,Tsujimoto M

    更新日期:2015-10-06 00:00:00

  • Characterization of recombinant Saccharomyces cerevisiae manganese-containing superoxide dismutase and its H30A and K170R mutants expressed in Escherichia coli.

    abstract::All known Mn-containing superoxide dismutases (MnSODs) have a highly conserved histidine (His-30 in Escherichia coli FeSOD) in the active-site channel, and nearly all have an active-site arginine (Arg-170) that has been proposed to play a combined structural and functional role [Chan et al., Arch. Biochem. Biophys. 27...


    pub_type: 杂志文章


    authors: Borders CL Jr,Bjerrum MJ,Schirmer MA,Oliver SG

    更新日期:1998-08-11 00:00:00

  • Absolute action spectrum of E-FADH2 and E-FADH2-MTHF forms of Escherichia coli DNA photolyase.

    abstract::Escherichia coli DNA photolyase mediates photorepair of pyrimidine dimers occurring in UV-damaged DNA. The enzyme contains two chromophores, 1,5-dihydroflavin adenine dinucleotide (FADH2) and 5,10-methenyltetrahydrofolylpolyglutamate (MTHF). To define the roles of the two chromophores in the photochemical reaction(s) ...


    pub_type: 杂志文章


    authors: Payne G,Sancar A

    更新日期:1990-08-21 00:00:00

  • Structural homology discloses a bifunctional structural motif at the N-termini of G alpha proteins.

    abstract::In a family of proteins, often the three-dimensional structure has been experimentally determined only for one member or a few members of the family. Homology modeling can be used to model the structures of all other members of the family and thus allow comparison of these structures. This approach was applied to hete...


    pub_type: 杂志文章


    authors: Kosloff M,Elia N,Selinger Z

    更新日期:2002-12-10 00:00:00

  • The NBDY Microprotein Regulates Cellular RNA Decapping.

    abstract::Proteogenomic identification of translated small open reading frames in humans has revealed thousands of microproteins, or polypeptides of fewer than 100 amino acids, that were previously invisible to geneticists. Hundreds of microproteins have been shown to be essential for cell growth and proliferation, and many reg...


    pub_type: 杂志文章


    authors: Na Z,Luo Y,Schofield JA,Smelyansky S,Khitun A,Muthukumar S,Valkov E,Simon MD,Slavoff SA

    更新日期:2020-10-27 00:00:00

  • Dissection of events in the resistance to β-lactam antibiotics mediated by the protein BlaR1 from Staphylococcus aureus.

    abstract::A heterologous expression system was used to evaluate activation of BlaR1, a sensor/signal transducer protein of Staphylococcus aureus with a central role in resistance to β-lactam antibiotics. In the absence of other S. aureus proteins that might respond to antibiotics and participate in signal transduction events, w...


    pub_type: 杂志文章


    authors: Llarrull LI,Mobashery S

    更新日期:2012-06-12 00:00:00

  • Distribution of disulfide bonds in the two-disulfide intermediates in the regeneration of bovine pancreatic ribonuclease A: further insights into the folding process.

    abstract::The distribution of one-disulfide bonds in the two-disulfide intermediates in the oxidative refolding of bovine pancreatic ribonuclease A has been characterized. These two-disulfide intermediates were formed from the fully reduced denatured protein by oxidation with dithiothreitol, then blocked with AEMTS, purified by...


    pub_type: 杂志文章


    authors: Volles MJ,Xu X,Scheraga HA

    更新日期:1999-06-01 00:00:00

  • A novel DNA topoisomerase I inhibitor with different mechanism from camptothecin induces G2/M phase cell cycle arrest to K562 cells.

    abstract::DNA topoisomerase I (Top1) is an essential nuclear enzyme and a validated target for anticancer agent screening. In a previous study, we found that indolizinoquinoline-5,12-dione derivatives show significant biological activity against several human cancer cell lines. To understand their mechanism of inhibition of can...


    pub_type: 杂志文章


    authors: Wu N,Wu XW,Agama K,Pommier Y,Du J,Li D,Gu LQ,Huang ZS,An LK

    更新日期:2010-11-30 00:00:00

  • Computational Prediction and Design for Creating Iteratively Larger Heterospecific Coiled Coil Sets.

    abstract::A major biochemical goal is the ability to mimic nature in engineering highly specific protein-protein interactions (PPIs). We previously devised a computational interactome screen to identify eight peptides that form four heterospecific dimers despite 32 potential off-targets. To expand the speed and utility of our a...


    pub_type: 杂志文章


    authors: Crooks RO,Lathbridge A,Panek AS,Mason JM

    更新日期:2017-03-21 00:00:00

  • Probing structural differences between PrP(C) and PrP(Sc) by surface nitration and acetylation: evidence of conformational change in the C-terminus.

    abstract::We used two chemical modifiers, tetranitromethane (TNM) and acetic anhydride (Ac(2)O), which specifically target accessible tyrosine and lysine residues, respectively, to modify recombinant Syrian hamster PrP(90-231) [rSHaPrP(90-231)] and SHaPrP 27-30, the proteinase K-resistant core of PrP(Sc) isolated from brain of ...


    pub_type: 杂志文章


    authors: Gong B,Ramos A,Vázquez-Fernández E,Silva CJ,Alonso J,Liu Z,Requena JR

    更新日期:2011-06-07 00:00:00

  • Effect of membrane potential on band 3 conformation in the human erythrocyte membrane detected by triplet state quenching experiments.

    abstract::The triplet lifetime and absorption anisotropy decay of eosin-labeled band 3 was measured in resealed erythrocyte ghosts. Membrane potentials were generated by the addition of valinomycin in the presence of a K+ gradient. Neither negative nor positive membrane potentials had any detectable effect on the rotational dif...


    pub_type: 杂志文章


    authors: Wyatt K,Cherry RJ

    更新日期:1992-05-19 00:00:00

  • X-ray structures of Torpedo californica acetylcholinesterase complexed with (+)-huperzine A and (-)-huperzine B: structural evidence for an active site rearrangement.

    abstract::Kinetic and structural data are presented on the interaction with Torpedo californica acetylcholinesterase (TcAChE) of (+)-huperzine A, a synthetic enantiomer of the anti-Alzheimer drug, (-)-huperzine A, and of its natural homologue (-)-huperzine B. (+)-Huperzine A and (-)-huperzine B bind to the enzyme with dissociat...


    pub_type: 杂志文章


    authors: Dvir H,Jiang HL,Wong DM,Harel M,Chetrit M,He XC,Jin GY,Yu GL,Tang XC,Silman I,Bai DL,Sussman JL

    更新日期:2002-09-03 00:00:00

  • Effects of a surfactant-associated protein and calcium ions on the structure and surface activity of lung surfactant lipids.

    abstract::Previous studies have demonstrated that lung-specific proteins are associated with surfactant lipids, particularly the highly surface active subfraction known as tubular myelin. We have isolated a surfactant-associated protein complex with molecular weight components of 36 000, 32 000, and 28 000 and reassembled it wi...


    pub_type: 杂志文章


    authors: Hawgood S,Benson BJ,Hamilton RL Jr

    更新日期:1985-01-01 00:00:00

  • Dissecting cytochrome P450 3A4-ligand interactions using ritonavir analogues.

    abstract::Cytochrome P450 3A4 (CYP3A4) inhibitors ritonavir and cobicistat, currently administered to HIV patients as pharmacoenhancers, were designed on the basis of the chemical structure/activity relationships rather than the CYP3A4 crystal structure. To better understand the structural basis for CYP3A4 inhibition and the li...


    pub_type: 杂志文章


    authors: Sevrioukova IF,Poulos TL

    更新日期:2013-07-02 00:00:00

  • Structural analysis of N-linked oligosaccharides of equine chorionic gonadotropin and lutropin beta-subunits.

    abstract::Equine chorionic gonadotropin (eCG) and lutropin (eLH) are composed of alpha- and beta-subunits with an identical amino acid sequence but show different biological activities. To elucidate the molecular difference between these gonadotropins, the structure of the N-linked oligosaccharides of each beta-subunit was dete...


    pub_type: 杂志文章


    authors: Matsui T,Mizuochi T,Titani K,Okinaga T,Hoshi M,Bousfield GR,Sugino H,Ward DN

    更新日期:1994-11-29 00:00:00

  • The Protein-tyrosine-phosphatase SHP2 is phosphorylated on serine residues 576 and 591 by protein kinase C isoforms alpha, beta 1, beta 2, and eta.

    abstract::To study whether protein kinase C (PKC) isoforms can interact with protein-tyrosine-phosphatases (PTPs) which are connected to the insulin signaling pathway, we co-overexpressed PKC isoforms together with insulin receptor, docking proteins, and the PTPs SHP1 and SHP2 in human embryonic kidney (HEK) 293 cells. After ph...


    pub_type: 杂志文章


    authors: Strack V,Krützfeldt J,Kellerer M,Ullrich A,Lammers R,Häring HU

    更新日期:2002-01-15 00:00:00

  • Importance of tetrahedral intermediate formation in the catalytic mechanism of the serine proteases chymotrypsin and subtilisin.

    abstract::Two new inhibitors in which the terminal α-carboxyl groups of Z-Ala-Ala-Phe-COOH and Z-Ala-Pro-Phe-COOH have been replaced with a proton to give Z-Ala-Ala-Phe-H and Z-Ala-Pro-Phe-H, respectively, have been synthesized. Using these inhibitors, we estimate that for α-chymotrypsin and subtilisin Carlsberg the terminal ca...


    pub_type: 杂志文章


    authors: Petrillo T,O'Donohoe CA,Howe N,Malthouse JP

    更新日期:2012-08-07 00:00:00

  • Effects of 3 beta-[2-(diethylamino)ethoxy]androst-5-en-17-one on the synthesis of cholesterol and ubiquinone in rat intestinal epithelial cell cultures.

    abstract::The relationship between cholesterol and ubiquinone synthesis in rat intestinal epithelial cell cultures was examined by using 3 beta-[2-(diethylamino)ethoxy]androst-5-en-17-one hydrochloride (U18666A). Addition of U18666A to cells caused a greater than 90% inhibition of incorporation of [3H]acetate into cholesterol a...


    pub_type: 杂志文章


    authors: Sexton RC,Panini SR,Azran F,Rudney H

    更新日期:1983-12-06 00:00:00

  • Changes in protein conformation and stability accompany complex formation between human C1 inhibitor and C1-s.

    abstract::The fluorescence spectrum of C1 inhibitor (C1-Inh) in aqueous buffer has a maximum at 324 nm which shifts to 358 nm in 6.0 M guanidinium chloride (GdmC1), indicating that fluorescent tryptophans are buried in the native protein. When titrated with GdmC1, the fluorescence intensity, polarization, and emission maximum o...


    pub_type: 杂志文章


    authors: Lennick M,Brew SA,Ingham KC

    更新日期:1985-05-07 00:00:00

  • Detection, sizing, and quantitation of polyadenylated ribonucleic acid in the nanogram-picogram range.

    abstract::A method is described for using very high specific activity [3H]poly(deoxythymidylate) [[3H]poly(dT)] to detect, size, and quantiate subnanogram amounts of nonradioactive polyadenylated RNA. Short (approximately 100 nucleotides long) [3H]poly(dT) is hybridized to the poly(adenylate) [poly(A)] tracts in polyadenylated ...


    pub_type: 杂志文章


    authors: Tracy S,Kohne DE

    更新日期:1980-08-05 00:00:00

  • Photoreactions of the Histidine Kinase Rhodopsin Ot-HKR from the Marine Picoalga Ostreococcus tauri.

    abstract::The tiny picoalga, Ostreococcus tauri, originating from the Thau Lagoon is a member of the marine phytoplankton. Because of its highly reduced genome and small cell size, while retaining the fundamental requirements of a eukaryotic photosynthetic cell, it became a popular model organism for studying photosynthesis or ...


    pub_type: 杂志文章


    authors: Luck M,Velázquez Escobar F,Glass K,Sabotke MI,Hagedorn R,Corellou F,Siebert F,Hildebrandt P,Hegemann P

    更新日期:2019-04-09 00:00:00

  • Hydrolysis of plant cuticle by plant pathogens. Properties of cutinase I, cutinase II, and a nonspecific esterase isolated from Fusarium solani pisi.

    abstract::The properties of the homogeneous cutinase I, cutinase II, and the nonspecific esterase isolated from the extracellular fluid of cutin-grown Fusarium solani F. pisi (R.E. Purdy and P.E. Kolattukudy (1975), Biochemistry, preceding paper in this issue) were investigated. Using tritiated apple cutin as substrate, the two...


    pub_type: 杂志文章


    authors: Purdy RE,Kolattukudy PE

    更新日期:1975-07-01 00:00:00

  • Folding of the SAM aptamer is determined by the formation of a K-turn-dependent pseudoknot.

    abstract::The S-adenosylmethionine (SAM) riboswitch is one of the most recurrent riboswitches found in bacteria and has three known different natural aptamers. The Bacillus subtilis yitJ SAM riboswitch aptamer is organized around a four-way junction which is characterized by the presence of a pseudoknot and a K-turn motif. By r...


    pub_type: 杂志文章


    authors: Heppell B,Lafontaine DA

    更新日期:2008-02-12 00:00:00

  • A concerted structural transition in the plasminogen activator inhibitor-1 mechanism of inhibition.

    abstract::The inhibition mechanism of serpins requires a change in structure to entrap the target proteinase as a stable acyl-enzyme complex. Although it has generally been assumed that reactive center loop insertion and associated conformational change proceeds in a concerted manner, this has not been demonstrated directly. Th...


    pub_type: 杂志文章


    authors: Blouse GE,Perron MJ,Thompson JH,Day DE,Link CA,Shore JD

    更新日期:2002-10-08 00:00:00

  • A gene cluster for biosynthesis of the sesquiterpenoid antibiotic pentalenolactone in Streptomyces avermitilis.

    abstract::Streptomyces avermitilis, an industrial organism responsible for the production of the anthelminthic avermectins, harbors a 13.4 kb gene cluster containing 13 unidirectionally transcribed open reading frames corresponding to the apparent biosynthetic operon for the sesquiterpene antibiotic pentalenolactone. The advanc...


    pub_type: 杂志文章


    authors: Tetzlaff CN,You Z,Cane DE,Takamatsu S,Omura S,Ikeda H

    更新日期:2006-05-16 00:00:00

  • Interactions of pig liver methylenetetrahydrofolate reductase with methylenetetrahydropteroylpolyglutamate substrates and with dihydropteroylpolyglutamate inhibitors.

    abstract::Dihydrofolate and dihydropteroylpolyglutamates inhibit pig liver methylenetetrahydrofolate reductase. In all cases the inhibition is linearly competitive with respect to methylenetetrahydrofolate. The Ki values decrease with each additional glutamyl residue from one to six, from a value of 6.5 microM for dihydrofolate...


    pub_type: 杂志文章


    authors: Matthews RG,Baugh CM

    更新日期:1980-05-13 00:00:00