Structures of gangliosides from bovine adrenal medulla.

abstract：:The catalytic activity of protein-tyrosine phosphatases (PTPs) is mediated by a cysteine side chain which carries out a nucleophilic attack initiating the phosphate cleavage. Experimentally, it has been observed that the active site cysteine has a remarkably low pKa. In the present study, we have investigated the orig...

journal_title：Biochemistry

authors： Peters GH,Frimurer TM,Olsen OH

更新日期：1998-04-21 00:00:00

abstract：:Primase is an essential DNA replication enzyme in Escherichia coli and responsible for primer synthesis during lagging strand DNA replication. Although the interaction of primase with single-stranded DNA plays an important role in primer RNA and Okazaki fragment synthesis, the mechanism of DNA binding and site selecti...

journal_title：Biochemistry

authors： Khopde S,Biswas EE,Biswas SB

更新日期：2002-12-17 00:00:00

abstract：:We have developed a simple method for reconstituting pure, integral membrane proteins into phospholipid-protein vesicles. The method does not depend on use of detergents or sonication. It has been used successfully with three different types of integral membrane proteins: UDPglucuronosyltransferase (EC 2.4.1.17) from ...

journal_title：Biochemistry

authors： Scotto AW,Zakim D

更新日期：1985-07-16 00:00:00

abstract：:We have characterized a membrane protein containing residues P688-T762 of the integrin beta3 subunit, encompassing its transmembrane and cytoplasmic domains, by nuclear magnetic resonance spectroscopy. Under conditions in which it is monomeric in dodecylphosphocholine micelles, the protein consists mainly of alpha-hel...

journal_title：Biochemistry

authors： Li R,Babu CR,Valentine K,Lear JD,Wand AJ,Bennett JS,DeGrado WF

更新日期：2002-12-31 00:00:00

abstract：:The electron spin echo decay envelope for the blue copper protein, stellacyanin, and for a number of other Cu(II) complexes has been studied. Particular attention was given to the form of the "nuclear modulation" patterns, which show the effects of coupling between the electron spin and the neighboring nuclei. The env...

journal_title：Biochemistry

authors： Mims WB,Peisach J

更新日期：1976-08-24 00:00:00

abstract：:The fibrillation of misfolded neurodegenerative disease-related proteins has been extensively studied over the past few decades, but the initial oligomerization has been rarely examined even though some recent evidence indicated that small protein oligomers are more neurotoxic than long protein fibers. It is rather di...

journal_title：Biochemistry

authors： Wang Y,Wu C

更新日期：2017-12-19 00:00:00

abstract：:The human cytosolic sulfotransferases (SULTs) regulate hundreds, perhaps thousands, of small molecule metabolites and xenobiotics via transfer of a sulfuryl moiety (-SO3) from PAPS (3'-phosphoadenosine 5'-phosphosulfate) to the hydroxyls and primary amines of the recipients. In liver, where it is abundant, SULT1A1 eng...

journal_title：Biochemistry

authors： Cook I,Wang T,Leyh TS

更新日期：2015-10-06 00:00:00

abstract：:We report on the effect of the viscogenic agents glycerol and ficoll on the RNase T1 catalyzed turnover of GpA, GpC, GpU, and Torula yeast RNA. For wild-type enzyme, we find that the kcat/Km values for the transesterification of GpC and GpA as well as for the cleavage of RNA are inversely proportional to the relative ...

journal_title：Biochemistry

authors： Steyaert J,Wyns L,Stanssens P

更新日期：1991-09-03 00:00:00

abstract：:Design of a partially cysteine-depleted C98S/C239S/C377S/C468A cytochrome P450 3A4 mutant designated CYP3A4(C58,C64) allowed site-directed incorporation of thiol-reactive fluorescent probes into alpha-helix A. The site of modification was identified as Cys-64 with the help of CYP3A4(C58) and CYP3A4(C64), each bearing ...

journal_title：Biochemistry

authors： Tsalkova TN,Davydova NY,Halpert JR,Davydov DR

更新日期：2007-01-09 00:00:00

abstract：:Bovine liver rhodanese, which catalyzes the transfer of sulfur atoms between a variety of sulfur donor and sulfur acceptor substrates, is inhibited by metal cyanide complexes [Volini, M., Van Sweringen, B., & Chen, F.-Sh. (1978) Arch. Biochem. Biophys. 191, 205-215]. Crystallographic studies are described which reveal...

journal_title：Biochemistry

authors： Lijk LJ,Kalk KH,Brandenburg NP,Hol WG

更新日期：1983-06-07 00:00:00

abstract：:The mature form of subtilisin is an unusual example of a monomeric protein with a high kinetic barrier to folding and unfolding. Using site-directed mutagenesis of subtilisin BPN', we are attempting to determine the physical and energetic nature of the kinetic barrier. The high-affinity calcium-binding site A has been...

journal_title：Biochemistry

authors： Strausberg S,Alexander P,Wang L,Gallagher T,Gilliland G,Bryan P

更新日期：1993-10-05 00:00:00

abstract：:Nephrocalcin (NC), a calcium-binding glycoprotein of 14,000 molecular weight as a monomer, is known to inhibit the growth of calcium oxalate monohydrate (COM) crystals in renal tubules. We have isolated NC from bovine kidney tissue and purified into four isoforms, fractions A-D. NC-A and NC-B strongly inhibit the grow...

journal_title：Biochemistry

authors： Mustafi D,Nakagawa Y

更新日期：1996-11-26 00:00:00

abstract：:A cDNA coding for a P450 expressed in human lung was isolated from a lambda gt11 library constructed from human lung mRNA using a cDNA probe to rat P450 IVA1. The cDNA-deduced amino acid sequence of this P450, designated IVB1, consisted of 511 amino acids and had a calculated molecular weight of 59,558. The IVB1 amino...

journal_title：Biochemistry

authors： Nhamburo PT,Gonzalez FJ,McBride OW,Gelboin HV,Kimura S

更新日期：1989-10-03 00:00:00

abstract：:Glutamate mutase is one of a group of adenosylcobalamin-dependent enzymes that catalyze unusual isomerizations that proceed through organic radical intermediates generated by homolytic fission of the coenzyme's unique cobalt-carbon bond. These enzymes are part of a larger family of enzymes that catalyze radical chemis...

journal_title：Biochemistry

authors： Cheng MC,Marsh EN

更新日期：2007-01-23 00:00:00

abstract：:To avoid nonspecific and/or undesirable binding and reactivity of metal ions with cellular components, organisms have evolved metal-specific systems for trafficking proteins. Although systems differ, those handling soft metal ions such as Hg(2+), Cu(+), Zn(2+), etc., all utilize heavy metal-associated (HMA) proteins a...

journal_title：Biochemistry

authors： Ledwidge R,Hong B,Dötsch V,Miller SM

更新日期：2010-10-19 00:00:00

abstract：:Holliday junctions are central intermediates in the process of genetic recombination. These DNA molecules contain four double helices that flank a central branch point, so that each of the four strands that constitute the junction is associated with two different helices. Previously, we have shown that such branched j...

journal_title：Biochemistry

authors： Wang H,Seeman NC

更新日期：1995-01-24 00:00:00

abstract：:S-Glutathionylation is a redox-regulated modification that uncouples endothelial nitric oxide synthase (eNOS), switching its function from nitric oxide (NO) synthesis to (•)O2(-) generation, and serves to regulate vascular function. While in vitro or in vivo eNOS S-glutathionylation with modification of Cys689 and Cys...

journal_title：Biochemistry

authors： Chen CA,De Pascali F,Basye A,Hemann C,Zweier JL

更新日期：2013-09-24 00:00:00

abstract：:The complexity of rat liver poly (A)+ messenger RNA (mRNA) has been measured by analysis of the kinetics of hydridization with both complementary DNA (cDNA) and single copy DNA. The complementary DNA-poly(A)+ mRNA hybridization reaction demonstrates the existence of three abundance classes representing 18, 37, and 45%...

journal_title：Biochemistry

authors： Savage MJ,Sala-Trepat JM,Bonner J

更新日期：1978-02-07 00:00:00

abstract：:Helicases unwind double-stranded nucleic acids, remove secondary structures from single-stranded nucleic acids, and remove proteins bound to nucleic acids. For many helicases, the mechanisms for these different functions share the ability to translocate with a directional bias as a result of ATP binding and hydrolysis...

journal_title：Biochemistry

authors： Reynolds KA,Cameron CE,Raney KD

更新日期：2015-07-14 00:00:00

abstract：:The cystic fibrosis transmembrane conductance regulator forms an anion-selective channel. We previously showed that charge selectivity, the ability to discriminate between anions and cations, occurs near the cytoplasmic end of the channel. The molecular determinants of charge selectivity, however, are unknown. We inve...

journal_title：Biochemistry

authors： Guinamard R,Akabas MH

更新日期：1999-04-27 00:00:00

abstract：:Acyl carrier protein (ACP) is the central player in fatty acid (FA) biosynthesis. It covalently binds all FA intermediates and presents them to the enzymes needed for elongation. Bacterial ACP must interact with a large number of proteins, which raises the question of how different acyl-ACPs are recognized and disting...

journal_title：Biochemistry

authors： Chan DI,Tieleman DP,Vogel HJ

更新日期：2010-04-06 00:00:00

abstract：:The insulin hexamer is an allosteric protein which displays positive and negative cooperativity and half-site reactivity that is modulated by strong homotropic and heterotropic ligand binding interactions at two different loci. These loci consist of phenolic pockets situated on the dimer-dimer interfaces of T-R and R-...

journal_title：Biochemistry

authors： Huang ST,Choi WE,Bloom C,Leuenberger M,Dunn MF

更新日期：1997-08-12 00:00:00

abstract：:The structure of HIV protease (HIV Pr) bound to JE-2147 (also named AG1776 or KNI-764) is determined here to 1.09 A resolution. This highest-resolution structure for HIV Pr allows refinement of anisotropic displacement parameters (ADPs) for all atoms. Clustering based on the directional information in ADPs defines two...

journal_title：Biochemistry

authors： Reiling KK,Endres NF,Dauber DS,Craik CS,Stroud RM

更新日期：2002-04-09 00:00:00

abstract：:The structural determinants of substrate specificity of human liver cytochrome P450 2C8 (CYP2C8) were investigated using site-directed mutants chosen on the basis of a preliminary substrate pharmacophore and a three-dimensional (3D) model. Analysis of the structural features common to CYP2C8 substrates exhibiting a mi...

journal_title：Biochemistry

authors： Melet A,Marques-Soares C,Schoch GA,Macherey AC,Jaouen M,Dansette PM,Sari MA,Johnson EF,Mansuy D

更新日期：2004-12-14 00:00:00

abstract：:PhnZ utilizes a mixed valence diiron(II/III) cofactor and O2 to oxidatively cleave the carbon-phosphorus bond of (R)-2-amino-1-hydroxyethylphosphonic acid to form glycine and orthophosphate. The active site residues Y24 and E27 are proposed to mediate induced-fit recognition of the substrate and access of O2 to one of...

journal_title：Biochemistry

authors： Gama SR,Lo BSY,Séguin J,Pallitsch K,Hammerschmidt F,Zechel DL

更新日期：2019-12-31 00:00:00

abstract：:The solvent accessibility of thrombin in its substrate-free and substrate-bound forms has been compared by amide hydrogen/deuterium (H/(2)H) exchange. The optimized inhibitor peptide dPhe-Pro-Arg chloromethyl ketone (PPACK) was used to simulate the substrate-bound form of thrombin. These studies were motivated by the ...

journal_title：Biochemistry

authors： Croy CH,Koeppe JR,Bergqvist S,Komives EA

更新日期：2004-05-11 00:00:00

abstract：:Indolicidin is an antimicrobial cationic peptide with broad-spectrum activity isolated from bovine neutrophils. An indolicidin analogue CP-11, ILKKWPWWPWRRK-NH(2), with improved activity against Gram-negative bacteria had increased positive charge and amphipathicity while maintaining the short length of the parent mol...

journal_title：Biochemistry

authors： Rozek A,Powers JP,Friedrich CL,Hancock RE

更新日期：2003-12-09 00:00:00

abstract：:The Staphylococcus aureus sortase transpeptidase SrtA isoform is responsible for the covalent attachment of virulence and colonization-associated proteins to the bacterial peptidoglycan. SrtA utilizes two substrates, undecaprenol-pyrophosphoryl-MurNAc(GlcNAc)-Ala-D-isoGlu-Lys(epsilon-Gly(5))-D-Ala-D-Ala (branched Lipi...

journal_title：Biochemistry

authors： Kruger RG,Otvos B,Frankel BA,Bentley M,Dostal P,McCafferty DG

更新日期：2004-02-17 00:00:00

abstract：:The presence of high titers of anti-cardiolipin antibodies (ACA's) of autoimmune origin, which are known to bind to plasma beta2-glycoprotein I (aka apolipoprotein H), correlates clinically with autoimmune recurrent thrombosis. Soluble beta2-glycoprotein I binds to solid-phase ACA (immobilized on a surface plasmon res...

journal_title：Biochemistry

authors： Sem DS,Baker BL,Victoria EJ,Jones DS,Marquis D,Yu L,Parks J,Coutts SM

更新日期：1998-11-17 00:00:00

abstract：:Firefly luciferase is 80-90% inactivated within 3 h upon incubation with the adenine nucleotide analogue p-fluorosulfonylbenzoyl-5'-adenosine (FSBA). Although 4 mol of 14C-FSBA/mol of enzyme is irreversibly bound during inactivation, only 1 mol of 14C-FSBA appears to be specifically directed to an adenine nucleotide b...

journal_title：Biochemistry

authors： Lee Y,Esch FS,DeLuca MA

更新日期：1981-03-03 00:00:00